阅读说明：本技术 一种香菇寡肽的制备方法及用途 (Preparation method and application of lentinus edodes oligopeptide ) 是由 伍曾利 于 2020-12-29 设计创作，主要内容包括：本发明涉及生物医药技术领域,尤其涉及一种香菇寡肽的制备方法及用途。本发明提供的方法对香菇水提取物进行CTAB的处理后,依次经过碱性蛋白酶酶解、风味蛋白酶酶解、活性炭脱色后,取分子量为300～1000Da的部分,制得香菇寡肽。研究表明,采用本发明提供方法提取获得的寡肽,具有良好的收率和纯度。更重要的是,相对于其他提取方法获得的香菇肽,本发明所述方法制得的香菇寡肽具有显著的抗肿瘤活性,其效果与阳性药物(环磷酰胺)相当。(The invention relates to the technical field of biomedicine, in particular to a preparation method and application of lentinus edodes oligopeptide. According to the method, water extract of mushroom is subjected to CTAB treatment, and then sequentially subjected to alkaline protease enzymolysis, flavourzyme enzymolysis and activated carbon decoloration, and then the part with the molecular weight of 300-1000 Da is taken to prepare the mushroom oligopeptide. Research shows that the oligopeptide extracted by the method provided by the invention has good yield and purity. More importantly, compared with the lentinus edodes peptides obtained by other extraction methods, the lentinus edodes oligopeptide prepared by the method has obvious anti-tumor activity and the effect is equivalent to that of a positive medicament (cyclophosphamide).)

1. A preparation method of mushroom oligopeptide is characterized by comprising the following steps:

extracting Lentinus Edodes with water to obtain extractive solution;

concentrating the extracting solution until the content of solid matters is 30-50%, treating with CTAB, and centrifuging to obtain supernatant;

and sequentially carrying out alkaline protease enzymolysis, flavourzyme enzymolysis and activated carbon decoloration on the supernatant, and taking the part with the molecular weight of 300-1000 Da to prepare the lentinus edodes oligopeptide.

2. The method according to claim 1, wherein the shiitake mushroom is a stipe of dried shiitake mushroom.

3. The method according to claim 1 or 2, wherein the preparing of the extract liquid includes:

crushing dried lentinus edodes stems, adding water with the weight of 5-15 times, grinding to 200-300 meshes, extracting for 2 hours at 90-100 ℃, and filtering to obtain filtrate I and filter residues;

adding 5-12 times of water into the filter residue, extracting for 1.5 hours at 90-100 ℃, and filtering to obtain filtrate II;

mixing the filtrate I and the filtrate II, centrifuging for 5-30 min at 6000-20000 rpm, and taking the supernatant as an extracting solution.

4. The method of claim 1, wherein the CTAB treatment comprises:

and at room temperature, adding CTAB while stirring until the mass fraction is 0.5-1.0%, continuously stirring for 5min, and standing for 30-180 min.

5. The method according to claim 1, wherein the conditions for the alkaline protease hydrolysis include: adding alkaline protease with international unit specific activity of 5000-40000 per kg of the supernatant; the pH value is 8.5-9.0, and enzymolysis is carried out for 20-120 min at 45-55 ℃.

6. The method according to claim 1, wherein the conditions for enzymatic hydrolysis of the flavourzyme comprise: adding flavourzyme with international unit specific activity of 5000-40000 per kg of the supernate; the pH value is 6.5-7.5, and enzymolysis is carried out for 20-120 min at 45-55 ℃.

7. The preparation method according to claim 1, wherein the activated carbon decoloring conditions include: adding active carbon into the enzymatic hydrolysate after enzymolysis until the mass fraction is 0.1-1%, stirring and decoloring for 10-60min at 70 ℃.

8. The preparation method according to claim 1, wherein the method further comprises the step of concentrating the fraction with the molecular weight of 300-1000 Da until the solid content is 40% -55%, and then drying the fraction under vacuum at 40-80 ℃ under the vacuum degree of-0.08-0.1 MPa.

9. A shiitake oligopeptide produced by the production method according to any one of claims 1 to 8.

10. Use of the lentinus edodes oligopeptide prepared by the preparation method of any one of claims 1-8 in preparing a medicament for treating tumors.

Technical Field

The invention relates to the technical field of biomedicine, in particular to a preparation method and application of lentinus edodes oligopeptide.

Background

Lentinus edodes belongs to Basidiomycetes, Agaricales (Agaricales), Tricholoma, Lentinus edodes (Lentinus), and the academic name Lentinus edodes, which originates from China, is the second largest mushroom in the world, and is a rare edible fungus which is famous for a long time in China. The mushroom is cultivated in China at first, and the history of more than 800 years is available up to now. The mushroom is also a famous medicinal fungus in China. The medical scientists in the past have written on the property and function of Lentinus edodes. The daily herbal medicine is used as a medicine, and the medicine is as follows: the purple mushroom is named as Lentinus edodes on the mushroom raw tung tree, willow and hovenia dulcis thunb. Sweet in nature and taste and mild in nature. Has the effects of strengthening body resistance, tonifying deficiency, invigorating spleen, stimulating appetite, dispelling pathogenic wind, promoting eruption, eliminating phlegm, regulating qi-flowing, removing toxic substances, and resisting cancer. Can be used for treating asthenia, listlessness, anorexia, dyspepsia, anemia, rickets, hypertension, hyperlipidemia, chronic hepatitis, night sweat, urinary incontinence, edema, measles, urticaria, poisonous mushroom poisoning, and tumor.

In recent years, researches show that the protein content of dried shiitake mushrooms is 19.3%, the amino acid types in the shiitake mushroom proteins are as many as 16, and the essential amino acids are 7. The current research on the active substances of the lentinus edodes mainly focuses on the research on lentinan. A large number of researches show that lentinan has an anti-tumor effect. In fact, besides polysaccharides, a large number of peptide substances exist in lentinus edodes, and these substances also have physiological activities in general, but the physiological activities of lenticin are not studied yet.

Disclosure of Invention

In view of the above, the technical problem to be solved by the present invention is to provide a preparation method and application of lentinus edodes oligopeptide with anti-tumor physiological activity.

The preparation method of the mushroom oligopeptide provided by the invention comprises the following steps:

extracting Lentinus Edodes with water to obtain extractive solution;

concentrating the extracting solution until the content of solid matters is 30-50%, treating with CTAB, and centrifuging to obtain supernatant;

and sequentially carrying out alkaline protease enzymolysis, flavourzyme enzymolysis and activated carbon decoloration on the supernatant, and taking the part with the molecular weight of 300-1000 Da to prepare the lentinus edodes oligopeptide.

According to the oligopeptide preparation method, the protein content of the extracting solution is preliminarily improved by the processes of extracting dry mushrooms and removing polysaccharides; the lentinus edodes oligopeptide is extracted from the extracting solution by the processes of double-enzyme enzymolysis, two-step nanofiltration and the like, the lentinus edodes protein is hydrolyzed into oligopeptide to the maximum extent, and the molecular weight of the lentinus edodes oligopeptide is controlled between 300 and 1000 daltons by the processes of nanofiltration purification and the like.

In the invention, the mushroom is a stipe of dry mushroom.

In the present invention, the preparation of the extract solution comprises: crushing dried lentinus edodes stems, adding water with the weight of 5-15 times, grinding to 200-300 meshes, extracting for 2 hours at 90-100 ℃, and filtering to obtain filtrate I and filter residues; adding 5-12 times of water into the filter residue, extracting for 1.5 hours at 90-100 ℃, and filtering to obtain filtrate II; mixing the filtrate I and the filtrate II, centrifuging for 5-30 min at 6000-20000 rpm, and taking the supernatant as an extracting solution.

In some embodiments, the preparing of the extraction liquid comprises: crushing dried lentinus edodes stems, adding 5 times of water by weight, grinding to 200 meshes, extracting for 2 hours at 90 ℃, and filtering to obtain filtrate I and filter residues; adding 5 times of water into the filter residue, extracting for 1.5 hours at 90 ℃, and filtering to obtain filtrate II; mixing the filtrate I and the filtrate II, centrifuging at 6000 rpm for 5min, and taking the supernatant as an extracting solution.

In some embodiments, the preparing of the extraction liquid comprises: crushing dried lentinus edodes stems, adding 10 times of water by weight, grinding to 250 meshes, extracting for 2 hours at 95 ℃, and filtering to obtain filtrate I and filter residues; adding 10 times of water by weight into the filter residue, extracting for 1.5 hours at 95 ℃, and filtering to obtain filtrate II; mixing the filtrate I and the filtrate II, centrifuging for 10min at 13000 r/min, and taking the supernatant as an extracting solution.

In some embodiments, the preparing of the extraction liquid comprises: crushing dried lentinus edodes stems, adding 15 times of water by weight, grinding to 300 meshes, extracting for 2 hours at 100 ℃, and filtering to obtain filtrate I and filter residues; adding 15 times of water into the filter residue, extracting for 1.5 hours at 100 ℃, and filtering to obtain filtrate II; mixing the filtrate I and the filtrate II, centrifuging for 15min at 20000 rpm, and taking the supernatant as an extracting solution.

In the present invention, the CTAB treatment comprises:

and at room temperature, adding CTAB while stirring until the mass fraction is 0.5-1.0%, continuously stirring for 5min, and standing for 30-180 min.

In the embodiment of the invention, when CTAB (cetyl trimethyl ammonium bromide) is added, an aqueous solution of CTAB is adopted, wherein the mass fraction of CTAB is 5-10%. Namely adding a CTAB aqueous solution with the mass fraction of 5-10% into the extracting solution until the mass fraction of CTAB in the extracting solution is 0.5-1.0%.

In some embodiments, the CTAB processing comprises: adding CTAB at room temperature while stirring to the mass fraction of 0.5%, stirring for 5min, and standing for 30 min.

In some embodiments, the CTAB processing comprises: adding CTAB at room temperature while stirring to the mass fraction of 0.75%, stirring for 5min, and standing for 30 min.

In some embodiments, the CTAB processing comprises: adding CTAB at room temperature while stirring until the mass fraction is 1.0%, continuing stirring for 5min, and standing for 30 min.

And in the step of centrifuging and taking supernatant after CTAB treatment, centrifuging for 5-30 min under the condition of 6000-20000 revolutions. In some embodiments, the centrifugation is performed at 6000/min for 5min, or at 20000/min for 15 min.

In the present invention, the conditions for the alkaline protease enzymolysis include: adding alkaline protease with international unit specific activity of 5000-40000 per kg of the supernatant; the pH value is 8.5-9.0, and enzymolysis is carried out for 20-120 min at 45-55 ℃. After the alkaline protease is subjected to enzymolysis, the method also comprises the step of inactivating the enzyme by the alkaline protease. And inactivating the enzyme for 5-15 min at 90-100 ℃.

In some embodiments, the conditions for alkaline protease enzymatic hydrolysis include: adding 5000 international units of specific activity alkaline protease into each kg of the supernatant; the pH value is 8.5, enzymolysis is carried out for 20min at 45 ℃, and then inactivation is carried out for 5min at 90 ℃.

In some embodiments, the conditions for alkaline protease enzymatic hydrolysis include: adding 21500 international unit specific activity alkaline protease per kg of the supernatant; the pH value is 9.0, enzymolysis is carried out at 50 ℃ for 70min, and then inactivation is carried out at 95 ℃ for 10 min.

In some embodiments, the conditions for alkaline protease enzymatic hydrolysis include: adding 40000 international units of specific activity alkaline protease per kg of the supernatant; performing enzymolysis at 55 deg.C for 120min at pH of 9.5, and inactivating at 100 deg.C for 15 min.

Before the enzymolysis of the flavourzyme, the solution after the enzymolysis and the enzyme deactivation of the alkaline protease is cooled to 20 ℃ to 40 ℃.

In the invention, the conditions of the flavourzyme enzymolysis comprise: adding flavourzyme with international unit specific activity of 5000-40000 per kg of the supernate; the pH value is 6.5-7.5, and enzymolysis is carried out for 20-120 min at 45-55 ℃. After the flavor protease is subjected to enzymolysis, the method also comprises the step of inactivating the flavor protease. And inactivating the enzyme for 5-15 min at 90-100 ℃.

In some embodiments, the conditions for flavourzyme enzymatic hydrolysis include: adding flavourzyme with 5000 international unit specific activity into each kg of the supernatant; the pH value is 6.5, enzymolysis is carried out for 20min at 45 ℃, and then inactivation is carried out for 5min at 90 ℃.

In some embodiments, the conditions for flavourzyme enzymatic hydrolysis include: adding about 21500 international units specific activity of flavourzyme per kg of the supernatant; the pH value is 7.0, enzymolysis is carried out for 70min at 50 ℃, and then inactivation is carried out for 10min at 95 ℃.

In some embodiments, the conditions for flavourzyme enzymatic hydrolysis include: adding 40000 international units of specific activity flavourzyme per kg of the supernatant; the pH value is 7.5, enzymolysis is carried out for 20min at 55 ℃, and then inactivation is carried out for 5min at 90 ℃.

In the present invention, the activated carbon decoloring conditions include: adding active carbon into the enzymatic hydrolysate after enzymolysis until the mass fraction is 0.1-1%, stirring and decoloring for 10-60min at 70 ℃. The decolorization step further comprises the step of filtering the filtrate.

In some embodiments, the conditions for decolorizing the activated carbon comprise: adding active carbon into the enzymolysis liquid until the mass fraction is 0.1%, stirring and decolorizing at 70 deg.C for 10 min.

In some embodiments, the conditions for decolorizing the activated carbon comprise: adding active carbon into the enzymolysis liquid until the mass fraction is 0.55%, stirring and decolorizing at 70 deg.C for 35 min.

In some embodiments, the conditions for decolorizing the activated carbon comprise: adding active carbon into the enzymolysis liquid until the mass fraction is 1%, stirring and decolorizing for 60min at 70 deg.C.

In the invention, the part with the molecular weight of 300-1000 Da comprises:

passing the decolorized liquid through a nanofiltration membrane with the molecular weight of 300 daltons, and taking trapped fluid;

and (4) passing the trapped fluid through a nanofiltration membrane with the molecular weight of 1000 daltons, and taking filtered fluid.

In the invention, after the part with the molecular weight of 300-1000 Da is taken, the step of concentrating until the solid content is 40% -55%, then carrying out vacuum drying at 40-80 ℃ under the vacuum degree of-0.08-0.1 MPa is included.

In some embodiments, the method further comprises concentrating the fraction with the molecular weight of 300-1000 Da until the solid content is 40%, and then vacuum drying at 40 ℃ under the vacuum degree of-0.08 MPa.

In some embodiments, the method further comprises concentrating the fraction with the molecular weight of 300-1000 Da until the solid content is 50%, and then vacuum drying at 60 ℃ under the vacuum degree of-0.09 MPa.

In some embodiments, the method further comprises concentrating the fraction with the molecular weight of 300-1000 Da until the solid content is 55%, and then vacuum drying the fraction at 80 ℃ under the vacuum degree of-0.1 MPa.

And after the vacuum drying, the method further comprises a sieving step, wherein the sieved sieve is 20-120 meshes, specifically 20 meshes, 80 meshes or 120 meshes.

The mushroom oligopeptide prepared by the preparation method is provided.

Experiments prove that the lentinus edodes oligopeptide prepared by the preparation method can obviously inhibit the growth of tumor of mice and prolong the service life of tumor-bearing mice.

The mushroom oligopeptide prepared by the preparation method is applied to preparing the medicine for treating tumors.

The invention also provides a medicine for treating tumors, which comprises the mushroom oligopeptide prepared by the preparation method.

The invention also provides a method for treating tumors, which comprises the step of administering the mushroom oligopeptide prepared by the preparation method. The administration method is oral administration or injection.

In the present invention, the tumor is a malignant tumor. In some embodiments, the tumor is selected from sarcoma, bladder cancer, blood cancer, bone cancer, brain cancer, breast cancer, central nervous system cancer, cervical cancer, colon cancer, endometrial cancer, esophageal cancer, gallbladder cancer, gastrointestinal cancer, genitourinary tract cancer, head cancer, kidney cancer, larynx cancerLiver cancer, lung cancer, cancer of muscle tissue, cancer of the neck, cancer of the oral or nasal mucosa, ovarian cancer, pancreatic cancer, prostate cancer, skin cancer, spleen cancer, small intestine cancer, large intestine cancer, stomach cancer, testicular cancer and/or thyroid cancer. In some embodiments, the sarcoma is leiomyosarcoma, liposarcoma, fibrosarcoma, or malignant mesothelioma. In the embodiment of the invention, S is₁₈₀Tumor cells A are used as experimental objects to construct tumor-bearing S₁₈₀NIH of A₆₁₅Pure mouse.

According to the method, water extract of mushroom is subjected to CTAB treatment, and then sequentially subjected to alkaline protease enzymolysis, flavourzyme enzymolysis and activated carbon decoloration, and then the part with the molecular weight of 300-1000 Da is taken to prepare the mushroom oligopeptide. Research shows that the oligopeptide extracted by the method provided by the invention has good yield and purity. More importantly, compared with the lentinus edodes peptides obtained by other extraction methods, the lentinus edodes oligopeptide prepared by the method has obvious anti-tumor activity and the effect is equivalent to that of a positive medicament (cyclophosphamide).

Detailed Description

The invention provides a preparation method and application of shiitake oligopeptide. The skilled person can use the contents to modify the process parameters appropriately. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.

The preparation method of the mushroom oligopeptide provided by the invention comprises the following steps:

(1) taking dried lentinus edodes stems, crushing, adding water with the mass of 5-15 times, grinding to 200-300 meshes by using a grinder, heating to 90-100 ℃, extracting for 2 hours, and filtering to obtain a filtrate I for later use;

(2) adding 5-12 times of water into filter residue, heating to 90-100 ℃, extracting for 1.5 hours, and filtering to obtain filtrate II for later use;

(3) collecting filtrate I and filtrate II, centrifuging for 5-30 min at 6000-20000 revolutions, and taking clear liquid;

(4) concentrating the solid to 30-50%, adding 5-10% cetyl trimethyl ammonium bromide solution under stirring to a final concentration of 0.5-1.0%, continuing stirring for 5-15 min, and standing at room temperature for 30-180 min;

(5) centrifuging at 6000-20000 rpm for 5-30 min, removing precipitate, and collecting clear liquid;

(6) adjusting the pH value of the clarified liquid to 8.5-9.0 by using NaOH solution, adding 5000-40000 international unit specific activity alkaline protease per kg, and performing enzymolysis at 45-55 ℃ for 20-120 min; after enzymolysis is finished, carrying out enzyme inactivation under the inactivation condition of 90-100 ℃ for 5-15 min, and cooling to 20-40 ℃ for later use;

(7) adjusting the pH value of the enzymolysis liquid to 6.5-7.5 by using NaOH solution, adding flavor protease with international unit specific activity of 5000-40000 per kg of liquid, carrying out enzymolysis for 20-120 min at 45-55 ℃, carrying out enzyme inactivation after the enzymolysis is finished, wherein the enzyme inactivation temperature is 90-100 ℃, the inactivation time is 5-15 min, and cooling to 20-40 ℃ for later use;

(8) adding 0.1-1% of active carbon into the enzymolysis liquid, heating to 70 ℃, stirring and decoloring for 10-60min, filtering, and taking clear liquid for later use;

(9) the clear liquid passes through a nanofiltration membrane with the molecular weight of 300 daltons, the trapped liquid is taken, the trapped liquid passes through a nanofiltration membrane with the molecular weight of 1000 daltons, and the filtered liquid is taken for standby;

(10) concentrating the filtrate until the solid content is 40-55%, the vacuum degree is-0.8-0.1 MPa, the temperature is 40-80 ℃, carrying out vacuum drying, crushing the dried substance, and sieving with a 20-120 mesh sieve to obtain the mushroom oligopeptide.

The test materials adopted by the invention are all common commercial products and can be purchased in the market. Wherein the flavor protease is Flavourzyme 500MG from Novoxil (China) investment limited company. The alkaline protease is Alcalase2.4L and comes from Novoxil (China) investment limited company. The dried mushroom is a product prepared by drying fresh mushroom, and the drying process comprises air drying, airing or baking and the like. The dried mushroom in the embodiment of the invention refers to the stipe of the dried mushroom, namely the stipe of the fruiting body in the center under the pileus of the mushroom. The room temperature in the invention refers to indoor temperature, and is preferably 18-30 ℃.

The invention is further illustrated by the following examples:

example 1:

taking 10kg of dried mushroom, crushing, adding 5 times of water, grinding to 200 meshes by using a grinder, heating to 90 ℃, extracting for 2 hours, and filtering to obtain 30.5kg of filtrate. Adding 5 times of water into the filter residue, heating to 90 deg.C, extracting for 1.5 hr, and filtering to obtain 35.1kg filtrate. Collecting the two filtrates, centrifuging at 6000 rpm for 5min to obtain clear liquid 55.2kg, concentrating the clear liquid to solid content of 30% to obtain concentrated liquid 7.4kg, adding 5% cetyl trimethyl ammonium bromide solution into the concentrated liquid under stirring to final concentration of 0.5%, stirring for 5min, and standing at room temperature for 30 min. Centrifuging at 6000 rpm for 5min, and removing precipitate to obtain clear liquid 6.5 kg.

Adjusting pH of the clarified liquid to 8.5 with NaOH solution, adding 5000 international specific activity alkaline protease per kg, and performing enzymolysis at 45 deg.C for 20 min; after enzymolysis is finished, enzyme inactivation is carried out under the inactivation condition of 90 ℃ for 5min, and the temperature is reduced to 20 ℃. Adjusting the pH value of the enzymolysis liquid to 6.5 by using NaOH solution, adding flavourzyme with 5000 international unit specific activity per kg of liquid, carrying out enzymolysis for 20min at 45 ℃, carrying out enzyme inactivation after the enzymolysis is finished, wherein the enzyme inactivation temperature is 90 ℃, the inactivation time is 5min, and cooling to 20 ℃. Adding 0.1% active carbon into the enzymolysis solution, heating to 70 deg.C, stirring for decolorizing for 10min, filtering, and collecting clear liquid 5.6 kg.

And (3) passing the clear liquid through a nanofiltration membrane with the molecular weight of 300 daltons to obtain 2.2kg of trapped liquid, passing the trapped liquid through a nanofiltration membrane with the molecular weight of 1000 daltons to obtain 1.6kg of filtered liquid, concentrating the filtered liquid until the solid content is 40%, performing vacuum drying at the vacuum degree of-0.08 MPa and the temperature of 40 ℃, crushing the dried substance, and passing the crushed substance through a 20-mesh screen to obtain 416g of lentinus edodes oligopeptide powder.

And (4) testing results:

the extraction yield of the oligopeptide: 4.16% (weight of oligopeptide powder/weight of dried mushroom powder X100)

Oligopeptide purity: 96.28% (reference standard: GB 18186-2000);

molecular weight range: 300-

Example 2:

taking 10kg of dried mushroom, crushing, adding 10 times of water, grinding to 250 meshes by using a grinder, heating to 95 ℃, extracting for 2 hours, and filtering to obtain 80.2kg of filtrate. Adding 10 times of water into the filter residue, heating to 95 deg.C, extracting for 1.5 hr, and filtering to obtain 85.5kg filtrate. Collecting the two filtrates, centrifuging at 13000 rpm for 10min to obtain clear liquid 132.8kg, concentrating the clear liquid to solid content of 35% to obtain concentrated liquid 8.0kg, adding 5% cetyl trimethyl ammonium bromide solution into the concentrated liquid under stirring to final concentration of 0.75%, stirring for 5min, and standing at room temperature for 30 min. Centrifuging at 6000 rpm for 5min, and removing precipitate to obtain 7.8kg of clarified liquid.

Adjusting pH of the clarified liquid to 9.0 with NaOH solution, adding 21500 international specific activity alkaline protease per kg, and performing enzymolysis at 50 deg.C for 70 min; after enzymolysis is finished, enzyme inactivation is carried out under the inactivation condition of 95 ℃ for 10min, and the temperature is reduced to 30 ℃. Adjusting the pH value of the enzymolysis liquid to 7.0 by using NaOH solution, adding flavor protease with specific activity of 21500 international units per kg of liquid, carrying out enzymolysis for 70min at 50 ℃, carrying out enzyme inactivation after the enzymolysis is finished, wherein the enzyme inactivation temperature is 95 ℃, the inactivation time is 10min, and cooling to 30 ℃. Adding 0.55% active carbon into the enzymolysis solution, heating to 70 deg.C, stirring for decolorizing for 35min, filtering, and collecting clear liquid 5.8 kg.

And (3) passing the clear liquid through a nanofiltration membrane with the molecular weight of 300 daltons to obtain 2.4kg of trapped liquid, passing the trapped liquid through a nanofiltration membrane with the molecular weight of 1000 daltons to obtain 1.7kg of filtered liquid, concentrating the filtered liquid until the solid content is 50%, performing vacuum drying at the vacuum degree of-0.09 MPa and the temperature of 60 ℃, crushing the dried substance, and passing the crushed substance through a 80-mesh screen to obtain 462g of lentinus edodes oligopeptide powder.

And (4) testing results:

the extraction yield of the oligopeptide: 4.62% (weight of oligopeptide powder/weight of dried mushroom powder X100)

Oligopeptide purity: 98.02% (reference standard: GB 18186-2000);

molecular weight range: 300-

Example 3:

taking 10kg of dried mushroom, crushing, adding 15 times of water, grinding to 300 meshes by using a grinder, heating to 100 ℃, extracting for 2 hours, and filtering to obtain 112kg of filtrate. Adding 15 times of water into the filter residue, heating to 100 deg.C, extracting for 1.5 hr, and filtering to obtain 116kg filtrate. Collecting the two filtrates, centrifuging at 20000 rpm for 15min to obtain 193kg of clear liquid, concentrating the clear liquid to solid content of 50% to obtain 8.6kg of concentrated liquid, adding 5% cetyl trimethyl ammonium bromide solution into the concentrated liquid under stirring to final concentration of 1.0%, stirring for 5min, and standing at room temperature for 30 min. Centrifuging at 20000 rpm for 15min, and removing precipitate to obtain 8.3kg of clarified liquid.

Adjusting pH of the clarified liquid to 9.5 with NaOH solution, adding 40000 international specific activity alkaline protease per kg, and performing enzymolysis at 55 deg.C for 120 min; after enzymolysis is finished, enzyme inactivation is carried out under the inactivation condition of 100 ℃ for 15min, and the temperature is reduced to 40 ℃. Adjusting the pH value of the enzymolysis liquid to 7.5 by using NaOH solution, adding flavor protease with specific activity of 40000 international units per kg of liquid, carrying out enzymolysis for 120min at 55 ℃, carrying out enzyme inactivation after the enzymolysis is finished, wherein the enzyme inactivation temperature is 100 ℃, the inactivation time is 15min, and cooling to 40 ℃. Adding 1% active carbon into the enzymolysis solution, heating to 70 deg.C, stirring for decolorizing for 60min, filtering, and collecting clear liquid 6.4 kg.

And (3) passing the clear liquid through a nanofiltration membrane with the molecular weight of 300 daltons to obtain 2.5kg of trapped liquid, passing the trapped liquid through a nanofiltration membrane with the molecular weight of 1000 daltons to obtain 1.8kg of filtered liquid, concentrating the filtered liquid until the solid content is 55%, performing vacuum drying at the vacuum degree of-0.1 MPa and the temperature of 80 ℃, crushing the dried substance, and passing the crushed substance through a 120-mesh screen to obtain 505g of lentinus edodes oligopeptide powder.

And (4) testing results:

the extraction yield of the oligopeptide: 5.05% (weight of oligopeptide powder/weight of dried mushroom powder X100)

Oligopeptide purity: 95.19% (reference standard: GB 18186-2000);

molecular weight range: 300-

Comparative example 1:

refer to patent ZL201110155206.8 for preparing Lentinus edodes polypeptide.

Comparative example 2:

taking 10kg of dried mushroom, crushing, adding 20 times of water, grinding to 120 meshes by using a grinder, heating to 80 ℃, extracting for 1 hour, and filtering to obtain 168kg of filtrate. Adding 18 times of water into the filter residue, heating to 80 ℃, extracting for 1 hour, and filtering to obtain 145kg of filtrate. Collecting the two filtrates, centrifuging at 5000 rpm for 31min to obtain 298kg of clarified liquid, concentrating the clarified liquid to 52% of solid to obtain 10.5kg of concentrated liquid, adding 6% cetyl trimethyl ammonium bromide solution into the concentrated liquid under stirring to final concentration of 0.8%, stirring for 16min, and standing at room temperature for 20 min. Centrifuging at 5000 rpm for 32min, and removing precipitate to obtain clear liquid 9.4 kg.

Adjusting pH of the clarified liquid to 8.0 with NaOH solution, adding alkaline protease with specific activity of 4000 International Unit per kg, and performing enzymolysis at 40 deg.C for 130 min; after enzymolysis is finished, enzyme inactivation is carried out under the inactivation condition of 85 ℃ for 20min, and the temperature is reduced to 40 ℃. Adjusting the pH value of the enzymolysis liquid to 6.0 by using NaOH solution, adding 4000 international unit specific activity flavourzyme per kg of liquid, carrying out enzymolysis for 130min at 40 ℃, carrying out enzyme inactivation after the enzymolysis is finished, wherein the enzyme inactivation temperature is 85 ℃, the inactivation time is 20min, and cooling to 40 ℃. Adding 1.5% active carbon into the enzymolysis solution, heating to 75 deg.C, stirring for decolorizing for 65min, filtering, and collecting clear liquid 6.4 kg. And (3) passing the clear liquid through a nanofiltration membrane with the molecular weight of 300 daltons to obtain 2.3kg of trapped liquid, passing the trapped liquid through a nanofiltration membrane with the molecular weight of 1000 daltons to obtain 1.6kg of filtered liquid, concentrating the filtered liquid until the solid content is 56%, performing vacuum drying at the vacuum degree of-0.1 MPa and the temperature of 100 ℃, crushing the dried substance, and passing the crushed substance through a 120-mesh screen to obtain 441g of lentinus edodes oligopeptide powder.

And (4) testing results:

the extraction yield of the oligopeptide: 4.41% (weight of oligopeptide powder/weight of dried mushroom powder X100)

Oligopeptide purity: 65.19% (reference standard: GB 18186-2000);

molecular weight range: 300-

Test examples: anti-tumor function of mushroom oligopeptide

1. Test drugs:

sample preparation: lentinus edodes oligopeptides prepared in embodiments 1, 2 and 3 of the present invention

Comparative example 1 (patent ZL201110155206.8) and comparative example 2

Lentinus edodes peptide with molecular weight greater than 1000 daltons

Lentinus edodes amino acid with molecular weight less than 300 dalton

Comparison products: cyclophosphamide.

2. Animals: NIH₆₁₅Pure mouse.

3. Tumor strain: SI (Standard interface)₁₈₀A. EAC and L₆₁₅Mice were provided by the institute for blood, institute of Chinese medical sciences.

4. Experimental methods and results

(1) Shiitake oligopeptide pair mouse S₁₈₀Influence of (2)

Taking NIH pure line mice with weight of 20 +/-2 g and dual purposes of male and female, inoculating according to the method of the national anti-tumor medicament in-vivo screening regulation, wherein the tumor source is S with good tumor growth after 6-9 days of inoculation₁₈₀A, ascites is taken out, and diluted by sterilized normal saline according to the ratio of l to 4 to prepare tumor cell suspension (5X 10)⁶Cells/ml). The mice were inoculated subcutaneously in the right axilla or intraperitoneally at 0.2ml/R, respectively.

Grouping randomly after 24h, each group containing 10 lentinus edodes oligopeptides, administering the lentinus edodes oligopeptides at 60mg/kg and cyclophosphamide at 60mg/kg by intraperitoneal or subcutaneous injection once a day, continuously administering the lentinus edodes oligopeptides for 10d by normal saline with the same volume as the control group, killing the subcutaneously inoculated mice the next day after stopping administration, taking out tumor masses, weighing, and calculating the tumor inhibition rate. The number of days of life was observed after the mice inoculated with the abdominal cavity had stopped taking the drug, and the life extension rate was calculated. The results are shown in the following table.

Table 3: shiitake oligopeptide pair mouse S₁₈₀Influence of (2)

Note: compared with the blank control group, the composition of the composition,^*P<0.01; compared with the lentinus edodes peptide samples with the molecular weight of more than 1000 daltons and the lentinus edodes amino acid samples with the molecular weight of less than 300 daltons of comparative examples 1 and 2,^#P<0.01。

table 4: effect of lentinus edodes oligopeptide on mouse S180A

Note: compared with the blank control group, the composition of the composition,^*P<0.01; compared with the lentinan with the molecular weight of more than 1000 daltons and the lentinan with the molecular weight of less than 300 daltons of comparative example 1 and comparative example 2,^#P<0.01。

as can be seen from the test results, the shiitake oligopeptide has remarkable effect on resisting mouse S₁₈₀Action of (1) against solid tumors S₁₈₀Or ascites type S₁₈₀(S₁₈₀A) Both have strong antagonistic action, and the effect is better than that of the lentinan peptide prepared in the patent ZL201110155206.8 and the lentinan peptide prepared in the comparative example 2, the lentinan peptide with the molecular weight of more than 1000 daltons and the lentinan amino acid with the molecular weight of less than 300 daltons.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that it is obvious to those skilled in the art that various modifications and improvements can be made without departing from the principle of the present invention, and these modifications and improvements should also be considered as the protection scope of the present invention.