阅读说明：本技术 一种促进水稻生根的微生物制剂及其制备方法和应用 (Microbial preparation for promoting rice rooting and preparation method and application thereof ) 是由 李凤兰 冯哲 张世宏 贺付蒙 冯艳忠 李柱刚 董文军 冯一新 徐永清 冯旭 袁强 于 2021-01-22 设计创作，主要内容包括：本发明公开了一种促进水稻生根的微生物制剂及其制备方法和应用。本发明的促进水稻生根的微生物制剂由复合微生物、木炭粉、葡萄糖和/或蔗糖、硫酸铵、磷酸氢二钾、硫酸镁和硼酸钠组成的原料制备而成。各原料组分按照重量计分别为：复合微生物20-30份、木炭粉30-50份、葡萄糖和/或蔗糖2-6份、硫酸铵1-5份、磷酸氢二钾1.0-2.0份、硫酸镁0.5-1.0份和硼酸钠0.1-0.5份。在应用本发明的微生物制剂时,先将其用清水稀释为悬浊液,然后分别在水稻秧苗的1叶1心期、2叶1心期、3叶1心期和拔节孕穗期,在稻田中均匀喷施一次悬浊液。本发明的微生物制剂能促进水稻秧苗根系生长,提高秧苗的素质,增加稻谷的产量。(The invention discloses a microbial preparation for promoting rice rooting and a preparation method and application thereof. The microbial preparation for promoting the rooting of the rice is prepared from raw materials consisting of compound microorganisms, charcoal powder, glucose and/or sucrose, ammonium sulfate, dipotassium hydrogen phosphate, magnesium sulfate and sodium borate. The raw material components by weight are respectively as follows: 20-30 parts of compound microorganism, 30-50 parts of charcoal powder, 2-6 parts of glucose and/or sucrose, 1-5 parts of ammonium sulfate, 1.0-2.0 parts of dipotassium hydrogen phosphate, 0.5-1.0 part of magnesium sulfate and 0.1-0.5 part of sodium borate. When the microbial preparation is applied, the microbial preparation is diluted into turbid liquid by using clear water, and then the turbid liquid is uniformly sprayed once in the rice field in the 1-leaf 1-heart stage, the 2-leaf 1-heart stage, the 3-leaf 1-heart stage and the jointing-pulling booting stage of rice seedlings respectively. The microbial preparation of the invention can promote the growth of rice seedling roots, improve the quality of rice seedlings and increase the yield of rice.)

1. A microbial preparation for promoting rice root growth is prepared from composite microorganism, charcoal powder, glucose and/or sucrose, ammonium sulfate, dipotassium hydrogen phosphate, magnesium sulfate and sodium borate.

2. The microbial formulation of claim 1, wherein: the raw materials comprise, by weight, 20-30 parts of the compound microorganism, 30-50 parts of the charcoal powder, 2-6 parts of the glucose and/or the sucrose, 1-5 parts of the ammonium sulfate, 1.0-2.0 parts of the dipotassium hydrogen phosphate, 0.5-1.0 part of the magnesium sulfate and 0.1-0.5 part of the sodium borate.

3. The microbial preparation of claim 1 or 2, wherein: the raw materials comprise, by weight, 23-30 parts of the compound microorganism, 35-50 parts of the charcoal powder, 2-5 parts of the glucose and/or the sucrose, 3-5 parts of the ammonium sulfate, 1.0-1.6 parts of the dipotassium hydrogen phosphate, 0.7-1.0 part of the magnesium sulfate and 0.1-0.4 part of the sodium borate.

4. The microbial preparation of claim 1 or 2, wherein: the raw materials comprise 26 parts of the compound microorganism, 42 parts of the charcoal powder, 2 parts of glucose and/or sucrose, 5 parts of ammonium sulfate, 1.4 parts of dipotassium hydrogen phosphate, 0.7 part of magnesium sulfate and 0.1 part of sodium borate by weight.

5. The microbial preparation of claim 1 or 2, wherein: the compound microorganism comprises trichoderma atroviride, bacillus pumilus, bacillus aryabhattai, azotobacter chroococcum and streptomyces rectus violaceus.

6. A process for the preparation of a microbial preparation according to any one of claims 1 to 5, comprising the steps of:

step 1, weighing each single bacterial powder according to a set proportion, and then mixing the single bacterial powders to prepare a composite microorganism;

and 2, weighing the compound microorganism, charcoal powder, glucose and/or sucrose, ammonium sulfate, dipotassium hydrogen phosphate, magnesium sulfate and sodium borate according to a set proportion, adding the raw material components together, stirring and mixing to obtain the microbial preparation.

7. The method of claim 6, wherein: the mesh number of the charcoal powder is more than or equal to 100 meshes.

8. Use of a microbial preparation according to any one of claims 1 to 5, wherein: diluting the microbial preparation into turbid liquid with clear water, and uniformly spraying the turbid liquid once in the rice field in the 1-leaf 1-heart stage, the 2-leaf 1-heart stage, the 3-leaf 1-heart stage and the jointing-removing and booting stage of the rice seedlings respectively.

9. Use according to claim 8, characterized in that: the dilution multiple of the microbial preparation is 30-50 times.

10. Use according to claim 8, characterized in that: the dosage of the microbial preparation is 15-30kg/hm per time²。

Technical Field

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a microbial preparation for promoting rice rooting as well as a preparation method and application thereof.

Background

With the development of society and the improvement of living standard of people, the contradiction between the reduction of cultivated land and the improvement of grain yield is increasingly prominent. The rice is the second major grain crop in China, and the improvement of the yield and the quality of the rice has great significance for the sustainable development of the society and the agriculture in China.

In the production process of rice, the large-area lodging of plants can cause serious yield reduction and reduce the quality of rice. One of the important reasons is the poor development of the root system of the rice seedling and the poor quality of the seedling.

Therefore, aiming at the problems, a preparation containing beneficial microorganisms is developed and is used for planting rice, so that the development and the growth of rice seedling root systems are promoted, and the preparation has great significance for improving the yield and the quality of the rice and promoting the agricultural sustainable development of China.

Disclosure of Invention

The invention aims to provide a microbial preparation for promoting rice rooting and a preparation method and application thereof, which promote the development and growth of rice seedling roots and improve the quality of rice seedlings in the rice planting process.

In one aspect, the invention provides a microbial preparation for promoting the growth of rice root systems, which is prepared from raw materials consisting of compound microorganisms, charcoal powder, glucose and/or sucrose, ammonium sulfate, dipotassium hydrogen phosphate, magnesium sulfate and sodium borate.

Preferably, the raw material consists of 20-30 parts of the compound microorganism, 30-50 parts of the charcoal powder, 2-6 parts of the glucose and/or the sucrose, 1-5 parts of the ammonium sulfate, 1.0-2.0 parts of the dipotassium hydrogen phosphate, 0.5-1.0 part of the magnesium sulfate and 0.1-0.5 part of the sodium borate by weight.

Preferably, the raw material comprises 23-30 parts of the compound microorganism, 35-50 parts of the charcoal powder, 2-5 parts of the glucose and/or the sucrose, 3-5 parts of the ammonium sulfate, 1.0-1.6 parts of the dipotassium hydrogen phosphate, 0.7-1.0 part of the magnesium sulfate and 0.1-0.4 part of the sodium borate by weight.

Preferably, the raw material consists of 26 parts of the complex microorganism, 42 parts of the charcoal powder, 2 parts of the glucose and/or sucrose, 5 parts of the ammonium sulfate, 1.4 parts of the dipotassium hydrogen phosphate, 0.7 part of the magnesium sulfate and 0.1 part of the sodium borate by weight.

Preferably, the complex microorganism comprises trichoderma atroviride, bacillus pumilus, bacillus aryabhattai, azotobacter chroococcum and streptomyces rectus violaceus.

In a second aspect, the present invention provides a method for preparing the microbial preparation, comprising the steps of:

step 1, weighing each single bacterial powder according to a set proportion, and then mixing the single bacterial powders to prepare a composite microorganism;

and 2, weighing the compound microorganism, charcoal powder, glucose and/or sucrose, ammonium sulfate, dipotassium hydrogen phosphate, magnesium sulfate and sodium borate according to a set proportion, adding the raw material components together, stirring and mixing to obtain the microbial preparation.

Preferably, the mesh number of the charcoal powder is more than or equal to 100 meshes.

In a third aspect, the invention also provides application of the microbial preparation, the microbial preparation is diluted into turbid liquid by using clear water, and then the turbid liquid is uniformly sprayed once in the rice field in the 1-leaf 1-heart stage, the 2-leaf 1-heart stage, the 3-leaf 1-heart stage and the jointing-in-booting stage of rice seedlings respectively.

Preferably, the microbial preparation is diluted by a factor of 30 to 50.

Preferably, the dosage of the microbial preparation is 15-30kg/hm each time²。

The microbial preparation of the invention is used for rice planting, can promote the development and growth of rice seedling roots, improve the quality of rice seedlings and increase the yield of rice.

Drawings

FIG. 1 shows the change in dry matter of rice after application of the microbial preparation of the invention;

FIG. 2 shows the SPAD change in rice flag leaf after application of the microbial preparation of the invention;

wherein CK represents a control group and T represents a test group.

Detailed Description

The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.

In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.

The invention prepares the microbial preparation for promoting the rooting of rice by proportioning compound microorganisms, charcoal powder, glucose and/or sucrose, ammonium sulfate, dipotassium hydrogen phosphate, magnesium sulfate and sodium borate according to a certain proportion. The charcoal powder is prepared from crop straw and/or forestry residues, and preferably has a mesh number of more than or equal to 100 meshes. Among the sucrose, brown sugar and soft sugar are preferable. The microbial preparation for promoting the rooting of the rice is used for planting the rice, can promote the development and the growth of rice seedling roots, improve the quality of the rice seedlings and increase the yield of the rice.

In one embodiment of the invention, the microbial preparation for promoting the rooting of the rice is prepared from raw materials consisting of compound microorganisms, charcoal powder, glucose and/or sucrose, ammonium sulfate, dipotassium hydrogen phosphate, magnesium sulfate and sodium borate. The specific preparation process comprises the following steps:

1) selecting Trichoderma atroviride (Trichoderma atroviride) powder and Bacillus pumilus (Bacillus pumilus)s) bacterial powder, Bacillus aryabhattai (Bacillus aryabhattai) bacterial powder, Azotobacter chroococcum (Azotobacter chrococcum) bacterial powder and Streptomyces violaceus (Streptomyces violaceoryces) bacterial powder, and then compounding five bacterial liquids according to a preset proportional relationship to obtain the composite microorganism, wherein the weight proportional relationship among the various microbial powders is preferably as follows: 10-25 parts of trichoderma atroviride powder, 10-20 parts of bacillus pumilus powder, 5-15 parts of bacillus aryabhattai powder, 10-20 parts of azotobacter chroococcum powder and 10-15 parts of streptomyces rectus violaceus powder. The five bacterial powders can be prepared by self, on one hand, trichoderma atroviride is fermented in a corresponding solid culture medium to obtain a solid culture, and then the solid culture is dried and crushed to prepare single-strain solid bacterial powder, wherein the bacterial content of the trichoderma atroviride is more than or equal to 3.0 multiplied by 10¹⁰cfu/g. On the other hand, bacillus pumilus, bacillus aryabhattai, azotobacter chroococcum and streptomyces rectus violaceus are fermented and cultured in corresponding liquid culture media to obtain bacterial liquids, and then the bacterial bodies are separated from the fermented culture and dried and concentrated to prepare single-strain solid bacterial powder. The bacterial contents of various single bacterial strains are respectively as follows: the bacterium content of the bacillus pumilus is more than or equal to 3.0 multiplied by 10¹⁰cfu/g, the bacterium content of Bacillus aryabhattai is more than or equal to 2.0 multiplied by 10¹⁰cfu/g, the bacterium content of azotobacter chroococcum is more than or equal to 4.0 multiplied by 10¹⁰cfu/g, the bacterial content of the streptomyces rectus violaceus is more than or equal to 2.5 multiplied by 10¹⁰cfu/g。

2) Weighing the compound microorganism, charcoal powder, glucose and/or sucrose, ammonium sulfate, dipotassium hydrogen phosphate, magnesium sulfate and sodium borate according to a preset weight proportion, then adding the raw materials together, stirring and uniformly mixing to obtain the microbial preparation for promoting the rooting of the rice. The weight proportions of the seven raw material components are respectively optimized as follows: 20-30 parts of compound microorganism, 30-50 parts of charcoal powder, 2-6 parts of glucose and/or sucrose, 1-5 parts of ammonium sulfate, 1.0-2.0 parts of dipotassium hydrogen phosphate, 0.5-1.0 part of magnesium sulfate and 0.1-0.5 part of sodium borate.

When rice is planted in a rice field, the microbial preparation is diluted into turbid liquid by clear water, and then the turbid liquid is uniformly sprayed once in the rice field in the 1-leaf 1-heart stage, the 2-leaf 1-heart stage, the 3-leaf 1-heart stage and the jointing-pulling booting stage of rice seedlings respectively. The microorganism isThe dilution factor of the preparation is preferably 30-50 times, and the dosage is preferably 15-30kg/hm each time²。

To help better understand the technical scheme of the present invention, the following examples are provided to illustrate the preparation process and application method of the microbial preparation for promoting rice rooting according to the present invention.

Example one

The raw materials of the microbial preparation for promoting the rooting of rice comprise, by weight, 20 parts of compound microorganisms, 30 parts of charcoal powder, 6 parts of glucose, 1 part of ammonium sulfate, 2.0 parts of dipotassium hydrogen phosphate, 0.5 part of magnesium sulfate and 0.5 part of sodium borate. Wherein the mesh number of the charcoal powder is 100 meshes. The composite microorganism is prepared by compounding trichoderma atroviride powder, bacillus pumilus powder, bacillus aryabhattai powder, azotobacter chroococcum powder and streptomyces rectus violaceus powder, wherein the five bacteria comprise, by weight, 10 parts of trichoderma atroviride powder, 14 parts of bacillus pumilus powder, 15 parts of bacillus aryabhattai powder, 17 parts of azotobacter chroococcum powder and 10 parts of streptomyces rectus violaceus powder. The inventor prepares five kinds of bacterial powder, and the specific process is as follows: on one hand, trichoderma aureoviride is fermented in a corresponding solid culture medium to obtain a solid culture, and then the solid culture is dried and crushed to prepare single-strain solid bacterial powder, wherein the bacterial content of the trichoderma aureoviride is more than or equal to 3.0 multiplied by 10¹⁰cfu/g. On the other hand, bacillus pumilus, bacillus aryabhattai, azotobacter chroococcum and streptomyces rectus violaceus are fermented and cultured in corresponding liquid culture media to obtain bacterial liquids, and then the bacterial bodies are separated from the fermented culture and dried and concentrated to prepare single-strain solid bacterial powder. The bacterial contents of various single bacterial strains are respectively as follows: the bacterium content of the bacillus pumilus is more than or equal to 3.0 multiplied by 10¹⁰cfu/g, the bacterium content of Bacillus aryabhattai is more than or equal to 2.0 multiplied by 10¹⁰cfu/g, the bacterium content of azotobacter chroococcum is more than or equal to 4.0 multiplied by 10¹⁰cfu/g, the bacterial content of the streptomyces rectus violaceus is more than or equal to 2.5 multiplied by 10¹⁰cfu/g。

The microbial preparation for promoting rice rooting of the embodiment is prepared by the following steps.

Step 1, adding trichoderma atroviride powder, bacillus pumilus powder, bacillus aryabhattai powder, azotobacter chroococcum powder and streptomyces rectus violaceus powder together, and mixing uniformly to obtain the compound microorganism.

And 2, adding the compound microorganism, charcoal powder, glucose, ammonium sulfate, dipotassium hydrogen sulfate, magnesium sulfate and sodium borate into the same mixing container, stirring and uniformly mixing to obtain the microbial preparation 1 for promoting the rooting of the rice.

When the microbial preparation 1 for promoting rice rooting is used for rice planting: according to 15kg/hm each time²The dosage of the microbial preparation 1 is that the microbial preparation 1 is diluted by 30 times with clear water to obtain turbid liquid, and then the turbid liquid is uniformly sprayed once in the rice field in the 1-leaf 1-heart stage, the 2-leaf 1-heart stage, the 3-leaf 1-heart stage and the jointing-pulling booting stage of the rice seedlings respectively.

Example two

The raw materials of the microbial preparation for promoting rice rooting in the embodiment comprise 23 parts of compound microorganisms, 36 parts of charcoal powder, 4 parts of brown sugar, 4 parts of ammonium sulfate, 1.0 part of dipotassium hydrogen phosphate, 0.8 part of magnesium sulfate and 0.3 part of sodium borate by weight. Wherein the mesh number of the charcoal powder is 110 meshes. The composite microorganism is prepared by compounding trichoderma atroviride powder, bacillus pumilus powder, bacillus aryabhattai powder, azotobacter chroococcum powder and streptomyces rectus violaceus powder, wherein the five bacteria are 15 parts of trichoderma atroviride powder, 20 parts of bacillus pumilus powder, 13 parts of bacillus aryabhattai powder, 10 parts of azotobacter chroococcum powder and 15 parts of streptomyces rectus violaceus powder respectively in parts by weight. The inventor prepares five kinds of bacterial powder, and the specific process is as follows: on one hand, trichoderma aureoviride is fermented in a corresponding solid culture medium to obtain a solid culture, and then the solid culture is dried and crushed to prepare single-strain solid bacterial powder, wherein the bacterial content of the trichoderma aureoviride is more than or equal to 3.0 multiplied by 10¹⁰cfu/g. On the other hand, bacillus pumilus, bacillus aryabhattai, azotobacter chroococcum and streptomyces rectus violaceus are fermented and cultured in corresponding liquid culture media to obtain bacterial liquids, and then the bacterial bodies are separated from the fermented culture and dried and concentrated to prepare single-strain solid bacterial powder. The bacterial contents of various single bacterial strains are respectively as follows: the bacterium content of the bacillus pumilus is more than or equal to 3.0 multiplied by 10¹⁰cfu/g, the bacterium content of Bacillus aryabhattai is more than or equal to 2.0 multiplied by 10¹⁰cfu/g, the bacterium content of azotobacter chroococcum is more than or equal to 4.0 multiplied by 10¹⁰cfu/g, the bacterial content of the streptomyces rectus violaceus is more than or equal to 2.5 multiplied by 10¹⁰cfu/g。

The microbial preparation 2 for promoting rooting of rice of this example was prepared in the same manner as in example one.

When the microbial preparation 2 for promoting rice rooting is used for rice planting: according to 20kg/hm each time²The dosage of the microbial preparation 2 is that the microbial preparation 2 is diluted by 40 times with clear water to obtain turbid liquid, and then the turbid liquid is uniformly sprayed once in the rice field in the 1-leaf 1-heart stage, the 2-leaf 1-heart stage, the 3-leaf 1-heart stage and the jointing-pulling booting stage of the rice seedlings respectively.

EXAMPLE III

The raw materials of the microbial preparation for promoting the rooting of rice comprise, by weight, 26 parts of compound microorganisms, 42 parts of charcoal powder, 2 parts of soft white sugar, 5 parts of ammonium sulfate, 1.4 parts of dipotassium hydrogen phosphate, 0.7 part of magnesium sulfate and 0.1 part of sodium borate. Wherein the mesh number of the charcoal powder is 115 meshes. The composite microorganism is prepared by compounding trichoderma atroviride powder, bacillus pumilus powder, bacillus aryabhattai powder, azotobacter chroococcum powder and streptomyces rectus violaceus powder, wherein the five bacteria comprise, by weight, 20 parts of trichoderma atroviride powder, 17 parts of bacillus pumilus powder, 8 parts of bacillus aryabhattai powder, 14 parts of azotobacter chroococcum powder and 12 parts of streptomyces rectus violaceus powder. The inventor prepares five kinds of bacterial powder, and the specific process is as follows: on one hand, trichoderma aureoviride is fermented in a corresponding solid culture medium to obtain a solid culture, and then the solid culture is dried and crushed to prepare single-strain solid bacterial powder, wherein the bacterial content of the trichoderma aureoviride is more than or equal to 3.0 multiplied by 10¹⁰cfu/g. On the other hand, bacillus pumilus, bacillus aryabhattai, azotobacter chroococcum and streptomyces rectus violaceus are fermented and cultured in corresponding liquid culture media to obtain bacterial liquids, and then the bacterial bodies are separated from the fermented culture and dried and concentrated to prepare single-strain solid bacterial powder. The bacterial contents of various single bacterial strains are respectively as follows: the bacterium content of the bacillus pumilus is more than or equal to 3.0 multiplied by 10¹⁰cfu/g, bacterial content of Bacillus aryabhattai≥2.0×10¹⁰cfu/g, the bacterium content of azotobacter chroococcum is more than or equal to 4.0 multiplied by 10¹⁰cfu/g, the bacterial content of the streptomyces rectus violaceus is more than or equal to 2.5 multiplied by 10¹⁰cfu/g。

The microbial preparation 3 for promoting rooting of rice of this example was prepared in the same manner as in example one.

When the microbial preparation 3 for promoting rice rooting is used for rice planting: according to a dosage of 25kg/hm each time²The dosage of the microbial preparation 3 is that the microbial preparation 3 is diluted by 40 times by clear water to obtain turbid liquid, and then the turbid liquid is uniformly sprayed once in the rice field in the 1-leaf 1-heart stage, the 2-leaf 1-heart stage, the 3-leaf 1-heart stage and the jointing-pulling booting stage of the rice seedlings respectively.

Example four

The raw materials of the microbial preparation for promoting the rooting of rice comprise, by weight, 30 parts of compound microorganisms, 50 parts of charcoal powder, 5 parts of a mixture of glucose and brown sugar, 3 parts of ammonium sulfate, 1.6 parts of dipotassium hydrogen phosphate, 1.0 part of magnesium sulfate and 0.4 part of sodium borate. Wherein the mesh number of the charcoal powder is 120 meshes. The composite microorganism is prepared by compounding trichoderma atroviride powder, bacillus pumilus powder, bacillus aryabhattai powder, azotobacter chroococcum powder and streptomyces rectus violaceus powder, wherein the five bacteria comprise, by weight, 25 parts of trichoderma atroviride powder, 10 parts of bacillus pumilus powder, 5 parts of bacillus aryabhattai powder, 20 parts of azotobacter chroococcum powder and 13 parts of streptomyces rectus violaceus powder. The inventor prepares five kinds of bacterial powder, and the specific process is as follows: on one hand, trichoderma aureoviride is fermented in a corresponding solid culture medium to obtain a solid culture, and then the solid culture is dried and crushed to prepare single-strain solid bacterial powder, wherein the bacterial content of the trichoderma aureoviride is more than or equal to 3.0 multiplied by 10¹⁰cfu/g. On the other hand, bacillus pumilus, bacillus aryabhattai, azotobacter chroococcum and streptomyces rectus violaceus are fermented and cultured in corresponding liquid culture media to obtain bacterial liquids, and then the bacterial bodies are separated from the fermented culture and dried and concentrated to prepare single-strain solid bacterial powder. The bacterial contents of various single bacterial strains are respectively as follows: the bacterium content of the bacillus pumilus is more than or equal to 3.0 multiplied by 10¹⁰cfu/g, the bacterium content of Bacillus aryabhattai is more than or equal to 2.0 multiplied by 10¹⁰cfu/g, the bacterium content of azotobacter chroococcum is more than or equal to 4.0 multiplied by 10¹⁰cfu/g, the bacterial content of the streptomyces rectus violaceus is more than or equal to 2.5 multiplied by 10¹⁰cfu/g。

The microbial preparation 4 for promoting rooting of rice of this example was prepared in the same manner as in example one.

When the microbial preparation 4 for promoting rice rooting is used for rice planting: according to 30kg/hm each time²The dosage of the microbial preparation 4 is that the microbial preparation 4 is diluted by 50 times with clear water to obtain turbid liquid, and then the turbid liquid is uniformly sprayed once in the rice field in the 1-leaf 1-heart stage, the 2-leaf 1-heart stage, the 3-leaf 1-heart stage and the jointing-pulling booting stage of the rice seedlings respectively.

In order to help better understand the technical scheme of the invention, the application of the microbial preparation 1 of the invention in rice planting is taken as a test example to illustrate the application effect of the invention.

Test example: application effect of microbial preparation in rice planting

The test field is located in Harbin city of Heilongjiang province, and the basic physical and chemical properties of the soil of the test field are that the pH value is 8.0, the organic matter is 2.6 percent, the total nitrogen is 1.1g/kg, the total phosphorus is 0.5g/kg, the total potassium is 18.6g/kg, the alkaline hydrolysis nitrogen is 85.3mg/kg, the quick-acting phosphorus is 28.4mg/kg, and the quick-acting potassium is 172.1 mg/kg. The experimental design 2 groups comprise 1 experimental group and 1 control group, each group of experimental designs 3 experimental cells, and the area of each experimental cell is 45m²All test cells are randomly distributed.

The microbial preparation 1 for promoting the rooting of rice prepared by the invention is applied to a test group. The specific application mode is as follows: according to 15kg/hm each time²The dosage of the microbial preparation 1 is that the microbial preparation 1 is diluted by 30 times with clear water to obtain turbid liquid, and then the turbid liquid is uniformly sprayed once in the rice field in the 1-leaf 1-heart stage, the 2-leaf 1-heart stage, the 3-leaf 1-heart stage and the jointing-pulling booting stage of the rice seedlings respectively. The control group was prepared by replacing the microbial agents for promoting the rooting of rice with an equal amount of clear water, and the rest of the procedures were the same as those of the test group.

The method selects Longdao No. 21 as a test variety, and adopts artificial rice transplanting in the middle ten days of 5 months, wherein the row spacing is 13.3cm, the row spacing is 30cm, and 5 plants are planted in each hole. The same general management is adopted for each cell, and the cells are harvested in the last 10 months. At the leaf stage of rice seedling 3, the height of seedling, leaf age, leaf stiffness, stem base width, above-ground dry weight, root dry weight, weight-height ratio and root-cap ratio of rice were investigated from each plot, and the results are shown in Table 1.

TABLE 1

As can be seen from the data of table 1, the seedling height, leaf age, leaf stiffness, stem base width, overground part dry weight, root dry weight, weight height ratio and root cap ratio of the test group were increased by 9.1%, 4.4%, 11.3%, 10.4%, 14.7%, 10.0%, 7.3%, 9.3%, respectively, as compared to the control. The microbial preparation of the invention can promote the development and growth of rice seedling roots and improve the quality of rice seedlings.

In addition, the change of dry matter and the change of SPAD of flag leaf of rice were examined from each cell sample during the mature period of rice, and the results are shown in fig. 1 and fig. 2, respectively. The results in figure 1 show that the rice dry matter in the test group is increased by 14.2% compared to the control group; the results in FIG. 2 show that the SPAD of the rice flag leaves in the test group was increased by 5.2% compared to the control group. It is demonstrated that the application of the microbial preparation of the present invention can promote the growth of rice seedlings.

When the rice is harvested, each cell is randomly sampled to carry out indoor test, the effective spike number, the grain number per spike, the setting rate and the thousand-grain weight of each group are investigated, the rice yield of the cell is counted after the rice is harvested, and the result is shown in table 2.

TABLE 2

The results in table 2 show that, compared with the control, the effective ear number of the test group is increased by 2.4%, the grain number per ear is increased by 11.5%, the maturing rate is increased by 5.5%, the thousand kernel weight is increased by 1.3%, and the rice yield is increased by 7.8%. It is demonstrated that the application of the microbial preparation of the present invention can improve the yield of rice.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification, or any other related technical fields directly or indirectly, are included in the scope of the present invention.