阅读说明：本技术 鸡血藤及鸡血藤豆科类似药材的用途及提取方法 (Application and extraction method of caulis Spatholobi and similar medicinal materials of caulis Spatholobi and Leguminosae ) 是由 杨彩艳 王金花 周柳芳 元辉雄 黎飚 潘兴寿 韦宝敏 邓百路 史沛钊 顾建忠 蔡 于 2020-12-31 设计创作，主要内容包括：本发明公开了一种鸡血藤及鸡血藤豆科类似药材的应用,鸡血藤和/或鸡血藤豆科类似药材在制备治疗肌肉减少症或治疗动脉粥样硬化的药物中的用途。可单独采用一种或多种鸡血藤及鸡血藤豆科类似药材提取物或将其一种或多种上述提取物与其它药材配伍,实现多成分、多靶点、协同作用增强降血脂、改善血液流变学、促进肌细胞增殖等防治少肌症的同时兼顾防治心脑血管疾病、月经病、骨质疏松症、咳嗽症等药效。(The invention discloses application of caulis spatholobi and similar medicinal materials of caulis spatholobi leguminosae, and application of caulis spatholobi and/or similar medicinal materials of caulis spatholobi leguminosae in preparing a medicament for treating sarcopenia or atherosclerosis. One or more caulis Spatholobi and similar extract of caulis Spatholobi and Leguminosae family medicinal materials can be independently adopted or one or more of the above extracts can be compatible with other medicinal materials, so that the effects of preventing and treating sarcopenia, cardiovascular and cerebrovascular diseases, menstrual diseases, osteoporosis, cough and the like are realized while the effects of multiple components, multiple target spots and synergistic action are realized, the blood fat is enhanced, the hemorheology is improved, the muscle cell proliferation is promoted and the like are prevented and treated.)

1. An application of caulis Spatholobi and similar medicinal materials of caulis Spatholobi and Leguminosae is characterized in that caulis Spatholobi and/or similar medicinal materials of caulis Spatholobi and Leguminosae are used for preparing medicines for treating sarcopenia; or

Use of caulis Spatholobi and/or similar materials of caulis Spatholobi and Leguminosae in preparing medicine for treating atherosclerosis is provided.

2. The use of claim 1, wherein the caulis Spatholobi is an extract of caulis Spatholobi, and the similar materials of caulis Spatholobi in Leguminosae are extracts of similar materials of caulis Spatholobi in Leguminosae.

3. The use of claim 1 or 2, wherein the caulis Spatholobi and similar leguminous materials thereof comprise: caulis Spatholobi, caulis Sargentodoxae, caulis seu folium Callicarpae Formosanae, Indian Millettia, Kunming caulis Spatholobi, herba Dendrobii, herba Tetrastigmatis cuneatae, herba Tetrastigmatis Hypoglauci, caulis Spatholobi, herba Tetrastigmatis Hypoglauci, caulis et folium Mucunae Longipedunculatae, and caulis et folium Mucunae.

4. The use as claimed in claim 1 or 2, wherein the caulis Spatholobi and/or similar materials of caulis Spatholobi of Leguminosae are fresh or dry whole plants or any part thereof.

5. The use as claimed in claim 2, wherein the extract of caulis Spatholobi and similar materials of the family Leguminosae of caulis Spatholobi is prepared by conventional method.

6. The use as claimed in claim 2, wherein the extract of the similar medicinal materials of the family of the genus spatholobus and the family of the genus spatholobus comprises the following steps:

(1) preparing the extract

Soaking the raw materials in ethanol solutions with different concentrations at 20-60 deg.C for 2-72 hr, filtering, standing the filtrate for precipitation to obtain supernatant, mixing the supernatants with different concentrations; extracting the residue and precipitate with boiling water for 5-30min, filtering, standing the filtrate for precipitation, collecting supernatant, and mixing the supernatants; mixing the supernatant extracted by ethanol and the supernatant extracted by boiling water to obtain an extracting solution;

(2) drying

Concentrating and drying the extracting solution prepared in the step (1) to obtain a dry extract product.

7. The use according to claim 6, wherein the step (1) comprises sequentially impregnating the raw material with 90%, 50% and 10% ethanol solutions by mass; the raw materials and ethanol solution are soaked in the soaking solution at a ratio of 1-3L ethanol solution per kg raw materials, and the soaking solution is stirred once every 0.5-3h for 5-30 min.

8. The use of the method as claimed in claim 6, wherein after the clarified liquid is prepared in the step (1), a decolorizing agent is added into the clarified liquid for impurity removal and decolorization, wherein the decolorizing agent comprises activated carbon and activated clay, and the mass ratio of the activated carbon to the activated clay is 1: 5-15.

9. The use as claimed in claim 6, wherein the concentration and drying in step (2) comprises placing the clarified liquid of step (1) in a vacuum concentration device for vacuum concentration at 50-55 deg.C, and placing the concentrated liquid in a vacuum drying oven for vacuum drying at 30-55 deg.C for 40-60h under a vacuum degree of-0.1-0.05 MPa to obtain dried extract.

10. Use according to claim 9, characterized in that in step (2) the vacuum is between-0.06 and-0.05 MPa, the temperature is maintained between 50 and 55 ℃, and the concentration is 10 to 50 times.

Technical Field

The invention relates to the technical field of traditional Chinese medicine application, and in particular relates to application and an extraction method of similar medicinal materials of spatholobus stem and leguminous spatholobus stem.

Background

The traditional Chinese medicine caulis Spatholobi is the rhizome of Spatholobus triloba (Spatholobus) of Spatholobus genus, is bitter and sweet in taste, slightly warm in nature, enters liver and kidney channels, has the effects of activating blood, replenishing blood, regulating menstruation, relieving pain, relaxing tendons, activating collaterals and the like, and is mainly used for treating diseases such as soreness and pain of waist and knees, numbness, paralysis, irregular menstruation and the like. Similar medicinal materials of caulis Spatholobi in the family of Leguminosae include Spatholobus (Spatholobus) caulis Sargentodoxae (Sinensis), Millettia (Millettia) Hainan Millettia (Millettia), Millettia (Millettia) Hainan Millettia (Millettia) Millettia (Pachylomia), Millettia speciosa (Millettia) Merr (Millettia speciosa (Millettia) pers., Millettia speciosa (D.Mey.) pers.), Millettia speciosa (D.C.Merr., Millettia speciosa (D.C., Millettia speciosa (D.D.) Merr., Millettia (D.) Merr. ex (D.C., Millettia) Merr. and Millettia speciosa (D.C.), and three varieties of Millettia speciosa (D.C., Millettia) Merr.) nakai) Millettia (D.) Merr. in Millettia (Millettia) and Millettia (Millettia) Merr. in Millettia (, The application of the traditional Chinese medicine composition in menstrual diseases such as dysmenorrheal and amenorrhea, cardiovascular and cerebrovascular diseases and anemia is wide, but no report for sarcopenia exists.

Sarcopenia (Sarcopenia), also clinically referred to as "skeletal muscle senescence" or "Sarcopenia", is a syndrome caused by a sustained loss of skeletal muscle mass, a decline in strength and function, a decline in skeletal muscle volume and function associated with aging, is a geriatric syndrome; skeletal muscle is the motive force of a human motion system, muscle aging and atrophy are important signs of human aging, and the problems of fracture, joint injury and the like are very easily caused; elderly suffering from sarcopenia have difficulty standing, slow walking, and are prone to fall and fracture, but sarcopenia also affects organ function and may cause heart and lung failure and even death.

Sarcopenia is closely related to poor prognosis such as reduced mobility, falling, disability and fatality rate, the most common causes are aging, tumor and malnutrition, and aging is the most important factor. At present, the treatment of sarcopenia mainly adopts functional exercise, nutritional intervention, hormone and the like, but the effect is poor and the adverse reaction is large.

The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.

Disclosure of Invention

The invention aims to provide application of caulis spatholobi and similar medicinal materials of caulis spatholobi leguminosae, and application of caulis spatholobi and/or similar medicinal materials of caulis spatholobi leguminosae as medicines for treating sarcopenia. The use of caulis Spatholobi and/or similar materials of caulis Spatholobi of Leguminosae as medicine for treating atherosclerosis is provided.

The invention also aims to provide a method for extracting the similar medicinal materials of the spatholobus stem and the leguminous family.

In order to realize the purpose, the invention provides an application of caulis spatholobi and similar medicinal materials of caulis spatholobi leguminosae, and an application of caulis spatholobi and/or similar medicinal materials of caulis spatholobi leguminosae in preparing a medicament for treating sarcopenia; or caulis Spatholobi and/or similar materials of caulis Spatholobi and Leguminosae in preparing medicine for treating atherosclerosis.

The medicine for treating sarcopenia contains caulis Spatholobi and/or similar materials of caulis Spatholobi and Leguminosae. The medicine for treating atherosclerosis contains caulis Spatholobi and/or similar materials of caulis Spatholobi and Leguminosae.

Preferably, in the above technical scheme, the caulis spatholobi is an extract of caulis spatholobi, and the similar medicinal material of the caulis spatholobi of the family leguminosae is an extract of the similar medicinal material of the family spatholobi of the family leguminosae.

Preferably, in the above technical scheme, the caulis spatholobi and similar medicinal materials of the family spatholobi comprise: caulis Spatholobi (caulis Spatholobi Spatholobus Trifoliata), caulis Sargentodoxae, caulis seu radix Tetrastigmatis Hypoglauci, Millettia championii, Kunming caulis Spatholobi, herba Dendrobii, Millettia pecuroides, Millettia speciosa, Millettia omeiana, Millettia speciosa, and Millettia speciosa.

Preferably, in the above technical scheme, the caulis spatholobi and/or similar medicinal materials of the caulis spatholobi family adopt fresh or dry products of whole plants or any parts thereof.

Preferably, in the above technical scheme, the extract of caulis Spatholobi and similar materials of caulis Spatholobi and Leguminosae is prepared by conventional method.

Preferably, in the above technical scheme, the extraction method of the extracts of caulis spatholobi and similar medicinal materials of the family spatholobus comprises the following steps:

(1) preparing the extract

Sequentially soaking the raw materials with ethanol solutions of different concentrations at 20-60 deg.C for 2-72 hr, filtering, standing the filtrate for precipitation to obtain supernatant, mixing the supernatants; extracting the residue and precipitate with boiling water for 5-30min for several times, filtering, standing the filtrate for precipitation to obtain supernatant, and mixing the supernatants; mixing the supernatant extracted by ethanol and the supernatant extracted by boiling water to obtain an extracting solution;

(2) drying

Concentrating the clear liquid prepared in the step (1), and drying to obtain a dry extract product.

Preferably, in the above technical solution, in the step (1), the raw material is sequentially immersed with 90% by mass, 50% by mass and 10% by mass of ethanol solution for 3 times, that is, the raw material is first immersed in 90% by mass of ethanol solution, the raw material is taken out and then immersed in 50% by mass of ethanol solution, and the raw material is taken out and then immersed in 10% by mass of ethanol solution. The raw materials and ethanol solution are soaked at a ratio of 1kg raw materials to 1-3L ethanol solution, and the raw materials and ethanol solution are stirred once every 0.5-3h in the soaking process for 5-30 min.

Preferably, in the technical scheme, after the clear liquid is prepared in the step (1), a decolorizing agent is added into the clear liquid for impurity removal and decolorization, wherein the decolorizing agent comprises activated carbon and activated clay, and the mixing mass ratio is 1: 5-15.

Preferably, in the above technical scheme, the concentrating and drying in step (2) is to put the clarified liquid in step (2) into a reduced pressure concentration device for reduced pressure concentration at 50-55 ℃, put the concentrated liquid into a reduced pressure drying oven for reduced pressure drying at 30-55 ℃ for 40-60h, and obtain the dry extract product with a vacuum degree of-0.1 to-0.05 MPa.

Preferably, in the technical scheme, the vacuum degree in the step (2) is-0.06 to-0.05 MPa, the temperature is kept at 50 to 55 ℃, and the concentration is 10 to 50 times.

The efficacy and behavior of spatholobus stem and similar leguminous medicinal materials enter meridians:

caulis Spatholobi (spatholobi, Lei Hua Dou, Lei Mai Teng) has bitter taste, slight sweet, warm nature, and liver and kidney meridian tropism, and has effects of promoting blood circulation, nourishing blood, regulating menstruation, relieving pain, relaxing muscles and tendons, activating collaterals, etc.; can be used for treating numbness of hand and foot, paralysis, rheumatalgia, menoxenia, dysmenorrhea, and amenorrhea.

The sargentgloryvine stem is sweet in taste and warm in nature, and has the effects of activating blood and enriching blood; can be used for treating anemia, amenorrhea, menoxenia, and arthralgia and myalgia.

Kunming caulis Spatholobi (Millettia dielsiana Hance) has bitter, astringent and sweet taste, warm nature, and has effects of replenishing blood, stopping bleeding, promoting blood circulation, and dredging channels and collaterals; can be used for treating blood deficiency, asthenia, tendons and bones due to old injury, menoxenia, amenorrhea, puerperal abdominal pain, lochiorrhea, hemorrhage, rheumatalgia, and traumatic injury.

The Millettia dielsiana is bitter in taste and warm in nature, and has the effects of promoting blood circulation, tonifying blood, and relaxing and activating channels and collaterals; can be used for treating anemia, puerperal asthenia, dizziness, menoxenia, rheumatalgia, and numbness of limbs.

Fengcheng caulis Spatholobi has bitter and sweet taste and warm nature, and has effects of promoting blood circulation, replenishing blood, relieving rigidity of muscles and activating collaterals; can be used for treating blood deficiency, asthenia, menoxenia, rheumatalgia, poliomyelitis, and traumatic injury.

The Mucuna birdwoodiana Tutcher (black Sargentodoxa cuneata) is bitter and astringent in taste and cool in nature, and has the effects of activating blood and enriching blood, clearing lung and moistening dryness, and clearing and activating channels and collaterals; can be used for treating anemia, menoxenia, cough due to lung heat, hemoptysis, soreness of waist and knees, rheumatalgia, numbness of limbs, and paralysis.

Caulis et folium Mucunae Indicae (caulis seu radix Mucunae Sempervirentis) has bitter and sweet taste and mild nature, has effects of promoting blood circulation, replenishing blood and dredging meridian passage, and can be used for treating anemia, leukopenia, menoxenia, numbness, paralysis and soreness of waist and legs.

Modern pharmacological research shows that the caulis spatholobi has the efficacies of expanding blood vessels, resisting platelet aggregation, prolonging blood coagulation time and promoting phosphorus metabolism. The sargentgloryvine stem can prolong the blood coagulation time of rabbit serum and inhibit platelet aggregation and erythrocyte aggregation of the rabbit. Fengcheng caulis Spatholobi can inhibit rabbit blood platelet aggregation and erythrocyte aggregation, has obvious inhibiting effect on formaldehyde arthritis, and has sedative and hypnotic effects when rat is injected with the tincture in abdominal cavity. The Millettia dielsiana has the drug effect of inhibiting platelet aggregation. Caulis Spatholobi can inhibit prostaglandin biosynthesis and platelet aggregation.

Compared with the prior art, the invention has the following beneficial effects:

(1) the medicine of the invention takes the caulis spatholobi and/or similar medicinal materials of the caulis spatholobi leguminosae as active ingredients, and other ingredients such as auxiliary materials acceptable in pharmaceutics are added, so that the medicine can be used for preparing the medicine for treating sarcopenia or preparing the medicine for treating atherosclerosis.

(2) Caulis Spatholobi and similar materials of caulis Spatholobi and Leguminosae all have effects of promoting blood circulation, nourishing blood, and relieving dysmenorrhea and activating collaterals etc. for preventing and treating myocardial hypofunction, menopathy, cardiovascular and cerebrovascular diseases, anemia and osteoporosis; and caulis Spatholobi and similar medicinal materials of caulis Spatholobi and Leguminosae have the strongest effect of reducing blood lipid, and caulis Spatholobi and Kunming caulis Spatholobi have stronger blood activating effect than caulis Spatholobi; in addition, caulis Sargentodoxae has effects of nourishing yin and moistening lung. Therefore, according to the compatibility concept that similar medicinal materials in the traditional Chinese medicine and pharmacy are mutually necessary and different medicinal materials are used, one or more caulis spatholobi and similar medicinal material extracts of the caulis spatholobi and the leguminous family can be independently adopted or one or more extracts of the caulis spatholobi and the similar medicinal materials of the caulis spatholobi and the leguminous family are compatible with other medicinal materials according to different clinical symptoms of patients such as cardiovascular and cerebrovascular diseases, anemia, menstrual diseases, osteoporosis, cough and the like, so that the effects of preventing and treating the sarcopenia, treating the cardiovascular and cerebrovascular diseases, the menstrual diseases, the osteoporosis, the cough and the like while realizing multi-component, multi-target point and synergistic action of enhancing the blood fat reduction, improving the hemorheology, promoting the proliferation of muscle cells.

(3) Caulis Spatholobi and similar medicinal materials of caulis Spatholobi and Leguminosae are easy to plant, so each medicinal material is common and easily available in traditional Chinese medicine and pharmacy, and has low cost and simple processing. Can be processed into pill, granule and decoction according to the disease condition and needs of patients, and is easy to take. Meanwhile, the compound preparation can realize prevention and treatment aiming at one or more symptoms of sarcopenia, which often has cardiovascular and cerebrovascular diseases, menstrual diseases, anemia, osteoporosis and the like; the medicinal materials belong to natural medicines, are non-toxic, warm or slightly warm or even or cool in nature, and the similar medicinal materials are mutually reinforced to realize long-term taking by people with body heat and body cold, so that the side effects and drug resistance brought by long-term taking of chemosynthetic medicines are avoided, and the medicinal composition accords with the characteristics of medicines for preventing and treating sarcopenia.

Drawings

FIG. 1 is a pathological section of rabbit aortic arch affected by different drugs;

FIG. 2 is a photograph of pathological sections of rabbit soleus muscle affected by the use of different drugs.

FIG. 3 is a cross-sectional view of pathological sections of rabbit soleus muscle using different drugs.

In fig. 1-3, A is normal group, B is high fat group, C is caulis Spatholobi group, and D is caulis Spatholobi group.

Detailed Description

The following detailed description of the present invention is provided in conjunction with the accompanying drawings, but it should be understood that the scope of the present invention is not limited to the specific embodiments.

Throughout the specification and claims, unless explicitly stated otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element or component but not the exclusion of any other element or component.

The extraction method for preparing the extracts of the caulis spatholobi and similar medicinal materials of the caulis spatholobi family comprises the following steps:

(1) preparing the extract

Taking caulis Spatholobi as raw material, cutting caulis Spatholobi into slices, sun drying or air drying, soaking 5kg of sequentially 90%, 50% and 10% ethanol at 20-60 deg.C for 3 times, filtering for 2-72 hr each time, soaking raw material and ethanol solution at a ratio of 1-3L ethanol solution per kg raw material, stirring once every 0.5-3 hr during soaking process, stirring for 5-30min, standing filtrate for precipitation, collecting supernatant, and mixing; extracting the residue and precipitate with boiling water for 2 times, each for 5-30min, filtering, standing the filtrate for precipitation to obtain supernatant, and mixing the supernatants; mixing the supernatant extracted with ethanol and the supernatant extracted with boiling water to obtain extractive solution.

(2) Removing impurities and decolorizing

Adding carbon with specific mass activity into the clarified liquid obtained in the step (1): 2000g of mixed decolorizer (1: 5-15) for active white clay is used for removing impurities and decolorizing, and the decolorized solution is in a light yellow color.

(3) Drying

And (3) placing the clarified liquid treated in the step (2) into a reduced pressure concentration device, concentrating at 50-55 ℃ under reduced pressure, placing the concentrated liquid into a reduced pressure drying oven, drying at 30-55 ℃ under reduced pressure for about 48 hours, wherein the vacuum degree is-0.08 to-0.1 MPa, and concentrating by 10-50 times to obtain a dried product of the caulis spatholobi extract.

Repeating the above extraction operations, and extracting similar medicinal materials of caulis Spatholobi of Leguminosae, such as caulis Sargentodoxae, caulis Piperis Futokadsurae, Millettia speciosa, Millettia championii, Millettia speciosa, and Millettia speciosa to obtain similar medicinal material extract of caulis Spatholobi of Leguminosae.

Example 1

The extraction method for preparing the extracts of the caulis spatholobi and similar medicinal materials of the caulis spatholobi family comprises the following steps:

(1) preparing the extract

Taking caulis Spatholobi as raw material, cutting caulis Spatholobi into slices, sun drying or air drying, soaking 5kg sequentially with 90%, 50% and 10% ethanol at 30 deg.C for 3 times, filtering for 72 hr each time, adding 1L ethanol solution into each kg raw material, stirring once every 1 hr in the soaking process, stirring for 15min, standing the filtrate for precipitation, collecting supernatant, and mixing; extracting the residue and precipitate with boiling water for 30min for 2 times, collecting supernatant, and mixing the supernatants; mixing the supernatant extracted with ethanol and the supernatant extracted with boiling water to obtain extractive solution.

(2) Removing impurities and decolorizing

Adding carbon with specific mass activity into the clarified liquid obtained in the step (1): 2000g of mixed decolorizer (1: 10) for activated clay is used for removing impurities and decolorizing, and the decolorized solution is in a light yellow color.

(3) Drying

And (3) placing the clarified liquid treated in the step (2) into a reduced pressure concentration device, concentrating at 50-55 ℃ under reduced pressure, placing the concentrated liquid into a reduced pressure drying oven, drying at 45 ℃ under reduced pressure for about 48 hours, wherein the vacuum degree is-0.08 MPa, and concentrating by 30 times to obtain a dried product of the caulis spatholobi extract.

Repeating the above extraction operations, and extracting similar medicinal materials of caulis Spatholobi of Leguminosae, such as caulis Sargentodoxae, caulis Piperis Futokadsurae, Millettia speciosa, Millettia championii, Millettia speciosa, and Millettia speciosa to obtain similar medicinal material extract of caulis Spatholobi of Leguminosae.

Example 2

The extraction method for preparing the extracts of the caulis spatholobi and similar medicinal materials of the caulis spatholobi family comprises the following steps:

(1) preparing the extract

Taking caulis Spatholobi as raw material, cutting caulis Spatholobi into slices, sun drying or air drying, soaking 5kg of the slices with 90%, 50% and 10% ethanol at 50 deg.C for 3 times, each time for 36 hr, filtering, soaking raw materials and ethanol solution at a ratio of adding 1.5L ethanol solution into each kg of raw materials, stirring once every 0.5 hr in the soaking process, stirring for 10min, standing the filtrate for precipitation, collecting supernatant, and mixing; extracting the residue with boiling water for 10min for 2 times, precipitating to obtain supernatant, and mixing the supernatants; mixing the supernatant extracted with ethanol and the supernatant extracted with boiling water to obtain extractive solution.

(2) Removing impurities and decolorizing

Adding carbon with specific mass activity into the clarified liquid obtained in the step (1): 2000g of mixed decolorant (1: 5) for active carclazyte is used for removing impurities and decoloring, and the decolored solution is in a light yellow color.

(3) Drying

And (3) placing the clarified liquid treated in the step (2) into a decompression concentration device, decompressing and concentrating at 50-55 ℃, placing the concentrated liquid into a decompression drying oven, decompressing and drying for about 48 hours at 30 ℃, wherein the vacuum degree is-0.1 MPa, and concentrating by 10 times to obtain a dried product of the caulis spatholobi extract.

Repeating the above extraction operations, and extracting similar medicinal materials of caulis Spatholobi of Leguminosae, such as caulis Sargentodoxae, caulis Piperis Futokadsurae, Millettia speciosa, Millettia championii, Millettia speciosa, and Millettia speciosa to obtain similar medicinal material extract of caulis Spatholobi of Leguminosae.

Example 3

The extraction method for preparing the extracts of the similar medicinal materials of the spatholobus stem and the spatholobus stem in the leguminosae comprises the following steps:

(1) preparing the extract

Taking caulis Spatholobi as raw material, cutting caulis Spatholobi into slices, sun drying or air drying, soaking 1kg sequentially with 90%, 50% and 10% ethanol at 60 deg.C for 3 times, each for 12 hr, filtering, soaking raw material and ethanol solution at a ratio of 3L ethanol solution per kg raw material, stirring once every 3 hr during soaking process, stirring for 30min, precipitating, collecting supernatant, and mixing; extracting the residue and precipitate with boiling water for 2 times (each for 90 min), collecting supernatant, and mixing the supernatants; mixing the supernatant extracted with ethanol and the supernatant extracted with boiling water to obtain extractive solution.

(2) Removing impurities and decolorizing

Adding carbon with specific mass activity into the clarified liquid obtained in the step (1): 2000g of mixed decolorizer (1: 15) for activated clay is used for removing impurities and decolorizing, and the decolorized solution is in a light yellow color.

(3) Drying

And (3) placing the clarified liquid treated in the step (2) into a reduced pressure concentration device, concentrating at 50-55 ℃ under reduced pressure, placing the concentrated liquid into a reduced pressure drying oven, drying at 55 ℃ under reduced pressure for about 48 hours, wherein the vacuum degree is-0.08 MPa, and concentrating by 20 times to obtain a dried product of the caulis spatholobi extract.

Repeating the above extraction operations, and extracting similar medicinal materials of caulis Spatholobi of Leguminosae, such as caulis Sargentodoxae, caulis Piperis Futokadsurae, Millettia speciosa, Millettia championii, Millettia speciosa, and Millettia speciosa to obtain similar medicinal material extract of caulis Spatholobi of Leguminosae.

Example 4

This example differs from example 1 in that in step (4) drying is carried out at 58 ℃ under reduced pressure for about 48h at a vacuum of-0.55 MPa and at 55 ℃ with 13-fold concentration.

Example 5

In vivo pharmacodynamic experiment of extracts of caulis Spatholobi and similar medicinal materials of caulis Spatholobi and Leguminosae

Test animals: 58 clean New Zealand rabbits with half of male and female and weight (2.25 plus or minus 0.25) kg. The experiment comprises a normal group, a caulis Spatholobi group, a caulis Sargentodoxae group, a Kunming caulis Spatholobi group and a model group, wherein each group comprises 8 animals, and the animals are raised in a single cage.

② preparing high-fat feed: the formula of the high-fat feed for the rabbits comprises 1.0% of cholesterol, 8% of lard, 4% of egg yolk powder and 87.0% of basic feed.

③ the test method: respectively mixing the different gradient extracting solutions of the medicinal materials, and concentrating under reduced pressure to obtain concentrated solution of each medicinal material extract, feeding normal feed to a blank group, feeding high-fat feed to an experimental group and orally administering, and feeding high-fat feed to a model group, wherein each feed is 120g per day; the experimental groups were fed continuously for 60 days with 10.4g of the herbal extract per day per dose. Blood is taken from the ear margin vein for 60d of experiment respectively to analyze blood indexes such as five items of blood fat, hemorheology, SOD and the like; after the drug effect experiment is finished, blood is taken from the ear vein, then the animal is dissected under urethane anesthesia, and the heart, the aorta and the soleus muscle are quickly taken out; cutting off aorta from the position where heart and aorta are connected and iliac artery branch, cutting off aorta longitudinally after separation, stripping adventitia to obtain aortic arch, fixing aortic arch and soleus muscle specimen with 10% neutral formalin solution, and treating Sudan IV staining and pathological examination.

Fourthly, test results:

see tables 1-2 and FIGS. 1-3. Table 1 shows the effect of the fractions of spatholobus stem, sargentgloryvine stem and Kunming spatholobus stem on the SOD and lipid metabolism of atherosclerotic rabbits. Table 2 shows the effect of caulis Spatholobi, caulis Spatholobi and Kunming caulis Spatholobi on the hemorheology of atherosclerotic rabbits.

As shown in FIG. 1, A is a normal group, B is a model group, C is a caulis Spatholobi group, and D is a caulis Sargentodoxae group. The aortic arch pathological section result shows that the aorta lumens of the rabbits in the normal group are smooth and complete, have no atheromatous lesion and no lipid plaque formation, the aorta intima of the rabbits in the model group is rough and uneven, has a large number of yellow and white striped lipid streaks and plaques, and is obviously raised towards the intima surface; the caulis Spatholobi group had no lipid plaque but had inferior smooth and intact lumen than normal group rabbit, and the caulis Spatholobi group had little lipid plaque, convex intimal surface but had a clear lesion degree compared with the model group (see FIG. 1).

As shown in FIGS. 2 and 3, A in FIGS. 2 and 3 is the normal group, B is the model group, C is the black blood group, and D is the caulis Spatholobi group. In fig. 3, the ordinate represents the proportion (%) of the muscle cross-sectional area. The pathological section result of the soleus muscle shows that the internal cracks of the soleus muscle in the model group are increased compared with those in the normal group, the muscle area is also obviously reduced compared with that in the normal group, the cracks of the caulis spatholobi group and the caulis spatholobi group are reduced compared with those in the normal group and the model group, the muscle area is increased, and the change of the caulis spatholobi is obvious compared with that of the caulis spatholobi; therefore, high-fat diet can affect the physiological motor function of soleus muscles, cause sarcopenia diseases and accelerate the development of diseases, and the caulis spatholobi extract can slow down or prevent sarcopenia, wherein the effect of preventing sarcopenia by the caulis spatholobi is better than that of caulis spatholobi.

TABLE 1 Effect of caulis Spatholobi, caulis Sargentodoxae and Kunming caulis Spatholobi on the SOD and lipid metabolism of atherosclerotic rabbits

Note: in comparison to the set of models,^★p<0.05，^☆p<0.01; in comparison with the normal group,^▲p<0.05，^△p<0.01 mmol/l, x. + -. s, n ═ 8, "-" not measured

From table 1, it can be seen that although the TG, TC and LDL-C of the spatholobus stem group, kunming spatholobus stem group and kadsura longepedunculata group are higher than the normal group but are significantly lower than the model group at the end of the experiment, the spatholobus stem group is the lowest; therefore, the caulis spatholobi, Kunming caulis spatholobi and caulis kadsurae Longipedunculatae all have the effect of preventing the blood fat from rising but have the best effect; from table 1, it can be seen that the content of SOD (superoxide dismutase) is not different among groups and has no statistical significance, and it is presumed that the three herbs have weak antioxidant activity or no antioxidant activity.

TABLE 2 Effect of caulis Spatholobi, caulis Sargentodoxae and Kunming caulis Spatholobi on the hemorheology of atherosclerotic rabbits

Note: in comparison to the set of models,^★p<0.05，^☆p<0.01; in comparison with the normal group,^▲p<0.05，^△p<0.01

in table 2, most of the indexes of the caulis spatholobi group, the Kunming caulis spatholobi group and the caulis spatholobi group are between the experimental group and the model group, the difference between the other two groups and the model group is more obvious than that of the caulis spatholobi group, but the difference between the two groups is not large, and most of the differences in hemorheology have statistical significance; therefore, the efficacy of the caulis spatholobi and Kunming caulis spatholobi in improving blood rheology is better than that of the caulis spatholobi, and the two groups have little difference in efficacy.

Example 6

In vitro pharmacodynamic experiment of caulis Spatholobi extract

(1) Cell culture

C is to be₂C₁₂Inoculating mouse myoblast into culture solution (containing 10% fetal calf serum), standing at 37 deg.C and 5% CO₂Culturing in an incubator.

(2) Promotion of C in vitro₂C₁₂Mouse myoblast proliferation assay

Collecting logarithmic phase cells, adjusting cell suspension concentration to 1 × 10⁵one/mL, and inoculated in a 96-well plate at 100uL per well. The plates were placed in an incubator (37 ℃, 5% CO)₂) Adding medicine after preculture for 24 h. The administration group (containing cell sap, culture solution, and drug, with drug concentration of 2mg/ml and 1) was set for the experiment5mg/ml, 1mg/ml and 0.5mg/ml) and blank (containing cell broth and culture broth) with 10uL of drug per well placed at 37 ℃ saturated humidity, 5% CO₂Culturing for 24h under the condition of (1). After 24h, 10uLCCK-8 solution was added to each well, and the incubation was continued for 4h, and the absorbance at 450nm was measured with a microplate reader.

(3) Results of the experiment

TABLE 3 caulis Spatholobi group C₂C₁₂Mouse myoblast proliferation effects

As shown in Table 3, the concentration of the extract of Spatholobus suberectus Dunn reaches 1.0mg/ml, which is better for promoting C₂C₁₂The mouse myoblast proliferates, and the effect was the best at a concentration of 2.0mg/ml among the four concentrations in the experiment, but the effect-concentration curve was broken at a concentration of 1.0 mg/ml.

The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.