阅读说明：本技术 一种婴幼儿配方奶粉及其制备方法 (Infant formula milk powder and preparation method thereof ) 是由 齐晓彦 刘振民 徐致远 苏米亚 陈文亮 贾宏信 揭良 于 2020-12-29 设计创作，主要内容包括：本发明公开了一种婴幼儿配方奶粉,该配方奶粉包括以下质量百分比的原料：原料乳15～35％、乳糖5～10％、脱盐乳清粉5～15％、乳清蛋白5～15％、混合植物油5～30％、磷脂1～5％、不饱和脂肪酸15～20％、益生元1～6％、婴儿维生素预混料0.01～0.1％和婴儿矿物质预混料0.01～0.1％。磷脂为大豆卵磷脂、蛋黄卵磷脂中的一种或多种；不饱和脂肪酸包括二十二碳六烯酸和花生四烯酸；益生元为低聚半乳糖和多聚果糖。混合植物油包括植物油和母乳脂替代脂；植物油为椰子油、玉米油、棕榈仁油、棕榈油及分提棕榈油、大豆油、菜籽油、低芥酸菜籽油、亚麻籽油、核桃油、高油酸葵花籽油、葵花籽油、稻米油中的一种或多种。本发明的婴幼儿配方奶粉更接近母乳,且D90值较大。(The invention discloses infant formula milk powder which comprises the following raw materials in percentage by mass: 15-35% of raw milk, 5-10% of lactose, 5-15% of desalted whey powder, 5-15% of whey protein, 5-30% of mixed vegetable oil, 1-5% of phospholipid, 15-20% of unsaturated fatty acid, 1-6% of prebiotics, 0.01-0.1% of infant vitamin premix and 0.01-0.1% of infant mineral premix. The phospholipid is one or more of soybean lecithin and yolk lecithin; unsaturated fatty acids include docosahexaenoic acid and arachidonic acid; the prebiotics are galacto-oligosaccharides and polyfructose. The mixed vegetable oil comprises vegetable oil and breast milk fat substitute fat; the vegetable oil is one or more of coconut oil, corn oil, palm kernel oil, palm oil and fractionated palm oil, soybean oil, rapeseed oil, low erucic acid rapeseed oil, linseed oil, walnut oil, high oleic acid sunflower oil, sunflower oil and rice oil. The infant formula powder disclosed by the invention is closer to breast milk, and the D90 value is larger.)

1. The infant formula milk powder is characterized by comprising the following raw materials in percentage by mass: 15-35% of raw milk, 5-10% of lactose, 5-15% of desalted whey powder, 5-15% of whey protein, 5-30% of mixed vegetable oil, 1-5% of phospholipid, 15-20% of unsaturated fatty acid, 1-6% of prebiotics, 0.01-0.1% of infant vitamin premix and 0.01-0.1% of infant mineral premix.

2. The infant formula of claim 1, wherein the phospholipid is one or more of soy lecithin, egg yolk lecithin;

the unsaturated fatty acids include docosahexaenoic acid and arachidonic acid;

the prebiotics are galacto-oligosaccharides and polyfructose.

3. The infant formula of claim 1, wherein said mixed vegetable oil comprises a vegetable oil and a human milk fat substitute fat;

the vegetable oil is one or more of coconut oil, corn oil, palm kernel oil, palm oil and fractionated palm oil, soybean oil, rapeseed oil, low erucic acid rapeseed oil, linseed oil, walnut oil, high oleic acid sunflower oil, sunflower oil and rice oil.

4. The infant formula milk powder with optimized milk fat formula according to claim 1, wherein the infant vitamin premix is prepared by compounding multiple vitamins of vitamin A, vitamin D, vitamin E, vitamin K1, vitamin B1, vitamin B2, nicotinamide, pantothenic acid, vitamin B6, vitamin B12, folic acid, biotin and vitamin C;

the infant mineral premix is prepared by compounding ferrous gluconate, manganese sulfate, copper gluconate, zinc sulfate, sodium selenite and potassium iodide.

5. A process for the preparation of an infant formula according to any one of claims 1 to 4, characterized in that it comprises the following steps:

(1) checking and accepting the raw milk and standardizing;

(2) mixing part of the mixed vegetable oil, part of the whey protein, part of the lactose and the phospholipid, emulsifying and homogenizing;

(3) mixing and completely dissolving raw milk, residual lactose, desalted whey powder, residual whey protein, residual mixed vegetable oil, prebiotics, infant vitamin premix and infant mineral premix, homogenizing, sterilizing, concentrating, and spray drying to obtain a material B;

(4) and (3) uniformly mixing the material B with unsaturated fatty acid to obtain the product.

6. The method for preparing infant formula according to claim 5, wherein in step (2), the homogenization pressure is 5MPa, and the homogenization is performed for 4 times.

7. The method for preparing the infant formula according to claim 5, wherein in the step (3), the homogenizing pressure is 15-25 MPa and the temperature is 50-65 ℃.

8. The method for preparing the infant formula according to claim 5, wherein in the step (3), the temperature for sterilization is 80-95 ℃ for 30-40 s.

9. The preparation method of the infant formula milk powder according to claim 5, wherein in the step (3), the spray drying is pressure spray drying, the feeding temperature of the pressure spray drying is 65-75 ℃, the air inlet temperature is 150-190 ℃, the air outlet temperature is 68-85 ℃, and the pressure is 150-300 bar.

10. The method for preparing infant formula according to claim 5, wherein in the step (3), the mixing is dry mixing for 2min at 1460 r/min;

the mixing temperature is 25-30 ℃, and the relative humidity of the mixing is 40-50%.

Technical Field

The invention belongs to the technical field of fermented foods, and particularly relates to infant formula milk powder and a preparation method thereof.

Background

Breast milk is the preferred food for healthy infants, providing various nutrients needed for the growth and development of infants. Breast milk contains 3% to 5% fat, often referred to as breast milk fat, which contains triglycerides (TAG, about 98%), phospholipids (about 1%), cholesterol (about 0.5%), and the like. Can provide 40% -50% of energy and essential fatty acids for infants of 0-6 months, and promote absorption of fat soluble vitamins.

Milk fat is a complex mixture of fats and oils, the most abundant triglycerides of which are 1, 3-dioleoyl-2-palmitoyl triglyceride (OPO) and 1-oleic-2-palmitic-3-linoleic triglyceride (OPL).

Human milk substitute fat in the Chinese market better simulates Chinese breast milk fat in terms of fatty acid composition, while great difference exists in the fatty acid three-dimensional structure, and the similarity with the Chinese breast milk fat is improved by adding OPO in some infant formula milk powder, but the Sn-2 site palmitic acid is still low. The human milk substitute fat and Chinese mother milk fat have larger difference in triglyceride composition, and the most obvious difference is that the content of OPL in the human milk substitute fat is lower, so that the human milk substitute fat in the Chinese market is developed based on the composition of foreign breast milk fat, and the difference of the components of the human milk substitute fat and Chinese mother milk fat is larger because the addition of OPL is not noticed. Therefore, the preparation of the high-similarity human milk substitute fat rich in OPL has wide prospect.

In addition, soybean phospholipid is generally added into the commercially available infant formula milk powder ingredients as an emulsifier, and the soybean phospholipid hardly contains protein and has no special function on the component composition, so that the soybean phospholipid is replaced, and the addition of phospholipid simulating human milk fat is more important for absorbing flowers of infants.

By optimizing the milk fat formula, the difference between the infant formula milk powder and the breast milk in terms of components and functions is reduced.

Disclosure of Invention

The invention aims to solve the technical problem of optimizing the fat composition of the infant formula milk powder, which is close to the composition similarity with Chinese mother milk fat. Developing a formula of infant formula milk powder closer to breast milk fat composition and a preparation method thereof.

On one hand, the invention provides infant formula milk powder which comprises the following raw materials in percentage by mass: 15-35% of raw milk, 5-10% of lactose, 5-15% of desalted whey powder, 5-15% of whey protein, 5-30% of mixed vegetable oil, 1-5% of phospholipid, 15-20% of unsaturated fatty acid, 1-6% of prebiotics, 0.01-0.1% of infant vitamin premix and 0.01-0.1% of infant mineral premix.

Further, the phospholipid is one or more of soybean lecithin and egg yolk lecithin;

the unsaturated fatty acids include docosahexaenoic acid and arachidonic acid;

the prebiotics are galacto-oligosaccharides and polyfructose.

Further, the mixed vegetable oil comprises vegetable oil and breast milk fat substitute fat;

the vegetable oil is one or more of coconut oil, corn oil, palm kernel oil, palm oil and fractionated palm oil, soybean oil, rapeseed oil, low erucic acid rapeseed oil, linseed oil, walnut oil, high oleic acid sunflower oil, sunflower oil and rice oil.

Further, the infant vitamin premix is prepared by compounding multiple components of vitamin A, vitamin D, vitamin E, vitamin K1, vitamin B1, vitamin B2, nicotinamide, pantothenic acid, vitamin B6, vitamin B12, folic acid, biotin and vitamin C;

the infant mineral premix is prepared by compounding ferrous gluconate, manganese sulfate, copper gluconate, zinc sulfate, sodium selenite and potassium iodide.

On the other hand, the invention also provides a preparation method of the infant formula milk powder, which comprises the following steps:

(1) checking and accepting the raw milk and standardizing;

(2) mixing part of the mixed vegetable oil, part of the whey protein, part of the lactose and the phospholipid, emulsifying and homogenizing;

(3) mixing and completely dissolving raw milk, residual lactose, desalted whey powder, residual whey protein, residual mixed vegetable oil, prebiotics, infant vitamin premix and infant mineral premix, homogenizing, sterilizing, concentrating, and spray drying to obtain a material B;

(4) and (3) uniformly mixing the material B with unsaturated fatty acid to obtain the product.

Further, in the step (2), the homogenization pressure is 5MPa, and the homogenization is carried out for 4 times.

Further, in the step (3), the homogenizing pressure is 15-25 MPa, and the temperature is 50-65 ℃.

Further, in the step (3), the sterilization temperature is 80-95 ℃ and the sterilization time is 30-40 s.

Further, in the step (3), the spray drying is pressure spray drying, the feeding temperature of the pressure spray drying is 65-75 ℃, the air inlet temperature is 150-190 ℃, the air exhaust temperature is 68-85 ℃, and the pressure is 150-300 bar.

Further, in the step (3), the mixing is dry mixing, the dry mixing time is 2min, and the rotating speed is 1460 r/min;

the mixing temperature is 25-30 ℃, and the relative humidity of the mixing is 40-50%.

The positive progress effects of the invention are as follows:

compared with the prior art, the invention has the beneficial effects that: the application adopts the mixture of mixed vegetable oil, whey protein powder, yolk phospholipid and lactose, according to the proportion of 3: the emulsifying effect is better when the proportion of 1 is used. The average particle size is relatively large; the particle size distribution is relatively wide; the corresponding value of D90 is also relatively large; whereas the zeta potential differs less. This application compares prior art and more approaches breast milk.

Detailed Description

In order to more clearly illustrate the technical solution of the present invention, the technical solution of the present invention will be further illustrated with reference to the following specific embodiments:

the invention provides infant formula milk powder, which adopts one of the technical schemes as follows:

an infant formula comprising as raw materials: 15-35% of raw milk, 5-10% of lactose, 5-15% of desalted whey powder, 5-15% of whey protein concentrate, 5-30% of mixed vegetable oil, 1-5% of phospholipid, 15-20% of unsaturated fatty acid, 1-6% of prebiotics, 0.01-0.1% of infant vitamin premix and 0.01-0.1% of infant mineral premix, wherein the percentages are mass percentages of the components in the raw materials of the infant formula milk powder.

The raw milk is preferably fresh cow's milk or goat's milk, preferably fresh cow's milk. Wherein the fresh milk is classified according to fat content, preferably whole milk or skim milk.

Wherein the raw milk preferably comprises skim milk and whole milk, wherein the total amount of skim milk and whole milk is preferably 15-35%, more preferably 20-27%, and most preferably 25%; the content of lactose is preferably 5 to 10%, more preferably 6 to 8%, and most preferably 8%; the content of the desalted whey powder is preferably 5 to 15%, more preferably 10 to 15%, and most preferably 13%; the content of the whey protein concentrate is preferably 5 to 15%, more preferably 8 to 10%, and most preferably 10%.

The content of the whey protein is preferably 4 to 20%, more preferably 8 to 10%, and most preferably 10%; the content of the mixed oil is preferably 5 to 30%, more preferably 20 to 30%, and most preferably 30%.

The raw materials of the invention also comprise mixed vegetable oil, including vegetable oil and breast milk fat replacing fat (modified vegetable oil).

The vegetable oil comprises coconut oil, corn oil, palm kernel oil, palm oil, fractionated palm oil, soybean oil, rapeseed oil, low erucic acid rapeseed oil, linseed oil, walnut oil, high oleic acid sunflower oil, sunflower seed oil and rice oil. Preferably corn oil, soybean oil, palm oil, coconut oil. Wherein the content of the vegetable oil is preferably 5 to 40%, more preferably 20 to 40%, and most preferably 25%. The content of the corn oil is preferably 1 to 10%, more preferably 2 to 8%, and preferably 5.5%; the content of the soybean oil is preferably 1 to 10%, more preferably 2 to 8%, and most preferably 6.4%; the content of the palm oil is preferably 10 to 50%, more preferably 20 to 40%, and preferably 34.4%; the content of the coconut oil is preferably 5-25%, more preferably 10-20%, and preferably 14.4%, wherein the percentage is the mass percentage of each component in the infant formula raw material.

The breast milk fat substitute fat comprises mixed fat of 1, 3-dioleic acid-2-palmitic acid triglyceride and 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride. Wherein the content of the breast milk fat-substitute fat is preferably 30 to 50%, more preferably 35 to 45%, and most preferably 39%.

The phospholipid comprises soybean lecithin and egg yolk lecithin. Preferably egg yolk lecithin. The content of the egg yolk lecithin is preferably 1 to 5%, more preferably 1 to 3%, and most preferably 2%.

The unsaturated fatty acids of the present invention include docosahexaenoic acid (DHA) and arachidonic acid (ARA). Wherein the content of docosahexaenoic acid (DHA) is preferably 1-10%, more preferably 2-8%, and preferably 7%. The content of arachidonic acid (ARA) is preferably 1 to 10%, more preferably 2 to 8%, and most preferably 7%.

In the present invention, the prebiotic is a prebiotic that is conventional in the art. Prebiotics (probiotics) are dietary supplements that beneficially affect the health of a host by selectively stimulating the growth and activity of one or a small number of bacteria in a colony, thereby improving the health of the host. The prebiotics are preferably galactooligosaccharides and polyfructose;

the infant vitamin premix is a conventional infant vitamin premix in the field, is generally prepared by compounding components such as vitamin A, vitamin D, vitamin E, vitamin K1, vitamin B1, vitamin B2, nicotinamide, pantothenic acid, vitamin B6, vitamin B12, folic acid, biotin, vitamin C and the like, is used in infant formula food type products, and mainly plays a role of a nutrition enhancer. The addition amount of the infant vitamin premix is the conventional addition amount in the field according with the corresponding national regulations. The preparation method of the infant vitamin premix is a conventional preparation method in the field or is commercially available.

The infant mineral premix is a conventional infant mineral premix in the field, is generally compounded by ferrous gluconate, manganese sulfate, copper gluconate, zinc sulfate, sodium selenite, potassium iodide and other components, is used in infant formula type products, mainly plays a role in nutrition fortification, and is in accordance with the national corresponding regulation and conventional in the field. The preparation method of the infant mineral premix is conventional in the art or is commercially available.

In order to solve the above technical problems, the second technical solution adopted by the present invention is: a method of preparing an infant formula as described above, comprising the steps of:

(1) checking and accepting the raw milk and standardizing;

(2) mixing the mixed vegetable oil with a mixture of partial whey protein powder, yolk phospholipid and lactose according to the proportion of 1:3, emulsifying and homogenizing for 4 times, wherein the secondary homogenizing pressure is 5 MPa;

(3) uniformly mixing and completely dissolving raw milk, lactose, desalted whey powder, concentrated whey protein, mixed grease, prebiotics, infant vitamin premix and infant mineral premix to obtain feed liquid B, homogenizing the obtained feed liquid B, sterilizing, concentrating, and spray drying to obtain material B;

(4) and uniformly mixing the material B with docosahexaenoic acid (DHA) and arachidonic acid (ARA) powder at the temperature of 25-30 ℃ and the relative humidity of 40-50% during mixing.

Wherein, after the raw milk in the step (1) is accepted, the protein, fat and milk solid are strictly detected according to the technical requirements, and if the raw milk is not accepted, the raw milk is standardized. The raw milk comprises 2.95-3.10% of protein, 3.10-3.30% of fat and 11.7% of total solid.

Wherein, the oil preparation in the step (2): injecting the emulsified vegetable oil into the oiling cylinder, starting the stirrer, heating to 40-50 ℃, and preparing to add into the batching tank.

Wherein the homogenizing pressure in the step (3) is preferably 15-25 MPa, and the temperature is 50-65 ℃. The sterilization is pasteurization and ultra-high temperature sterilization, preferably, the sterilization condition is that the temperature is 80-95 ℃ and the time is 30-40 seconds. The concentration is a conventional operation in the field, preferably a four-effect concentration process, and the solid concentration of the obtained concentrated milk is 40-50% (wt). The spray drying is a conventional operation in the field, preferably pressure spray drying, wherein the feeding temperature of the pressure spray drying is preferably 65-75 ℃, the air inlet temperature is preferably 150-190 ℃, the air exhaust temperature is preferably 68-85 ℃, and the pressure is preferably 150-300 bar.

The mixing method in step (4) is conventional in the art. The mixing method is preferably a two-stage or three-stage dilution method, i.e., first, docosahexaenoic acid (DHA) and arachidonic acid (ARA) powders are premixed with the materials at a ratio of 1:10 by volume, and then, three-stage dry mixing is performed. Dry mixing and mixing: the dry mixing operation time is 2min, the rotating speed is 1460r/min, and the mixed semi-finished product is stored in a closed temporary storage device. Wherein the temperature of the uniform mixing is preferably 20 to 30 ℃, more preferably 25 to 30 ℃, preferably 30 ℃, and the relative humidity is preferably 40 to 50%, more preferably 40 to 45%, preferably 40%.

The preparation method of the infant formula powder of the present invention preferably further comprises a packaging step, wherein the packaging step is performed by packaging and storing the infant formula powder in a composite plastic container or an iron can according to the conventional method in the field. The packaging step preferably further comprises evacuating the packaging container or filling the packaging container with an inert gas, such as an inert gas conventional in the art, preferably nitrogen or carbon dioxide, in a volume percentage, such that the amount of residual oxygen in the packaging container is less than 5%. The reagents and starting materials used in the present invention are commercially available.

On the basis of the common knowledge in the field, the preferred conditions can be combined randomly to obtain the preferred embodiments of the invention. On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention. On the basis of the common knowledge in the field, the preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.

The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.

The performance indexes of the raw materials meet the requirements of relevant quality standards. Other materials and equipment not specifically described are commercially available.

Example 1

An infant formula milk powder with an optimized milk fat formula is shown in table 1, and the preparation method comprises the following steps:

table 1 ingredient formulation of example 1

The formula of the infant formula as described above is prepared by a method comprising the steps of:

(1) checking and accepting the raw milk and standardizing;

(2) mixing the mixed vegetable oil with the mixture of whey protein powder, yolk phospholipid and lactose according to the proportion of 1:3, emulsifying and homogenizing for 4 times, wherein the secondary homogenizing pressure is 5 MPa;

(3) uniformly mixing and completely dissolving raw milk, lactose, desalted whey powder, concentrated whey protein, mixed oil (29%: 17%: 11%: 4%: 39%) and fat (29%: 17%: 11%: 4%: 39%) respectively), prebiotics, infant vitamin premix and infant mineral premix to obtain feed liquid B, homogenizing the obtained feed liquid B, sterilizing, concentrating and spray-drying to obtain the material B;

(4) uniformly mixing the material B with docosahexaenoic acid (DHA) and arachidonic acid (ARA) powder at 25-30 ℃ and 40-50% of relative humidity during mixing;

(5) and packaging and storing by adopting a composite plastic container or an iron can.

Specifically, after the raw milk in the step (1) is accepted, protein, fat and milk solids are strictly detected according to the technical requirements, and if the raw milk is not accepted, the raw milk is standardized. The raw milk comprises 2.95-3.10% of protein, 3.10-3.30% of fat and 11.7% of total solid.

Step (2), oil preparation: injecting the emulsified vegetable oil into the oiling cylinder, starting the stirrer, heating to 40-50 ℃, and preparing in the batching tank. Adding a small amount of water into the whey protein powder, the yolk phospholipid and the lactose, uniformly mixing, and pouring into a mixing tank to emulsify with the mixed oil. Wherein the ratio of the mixed grease to other raw material mixtures is 1: 3.

The homogenization in the step (3) is a conventional operation in the field, the homogenization pressure is preferably 15-25 MPa, and the temperature is 50-65 ℃. The sterilization is conventional operation in the field, such as pasteurization and ultra-high temperature sterilization, and preferably, the sterilization condition is that the temperature is 80-95 ℃ and the time is 30-40 seconds. The concentration is a conventional operation in the field, preferably a four-effect concentration process, and the solid concentration of the obtained concentrated milk is 40-50% (wt). The spray drying is a conventional operation in the field, preferably pressure spray drying, wherein the feeding temperature of the pressure spray drying is preferably 65-75 ℃, the air inlet temperature is preferably 150-190 ℃, the air exhaust temperature is preferably 68-85 ℃, and the pressure is preferably 150-300 bar.

The mixing method in step (4) is conventional in the art. The mixing method is preferably a two-stage or three-stage dilution method, i.e., first, docosahexaenoic acid (DHA) and arachidonic acid (ARA) powders are premixed with the materials at a ratio of 1:10 by volume, and then, three-stage dry mixing is performed. Dry mixing and mixing: the dry mixing operation time is 2min, the rotating speed is 1460r/min, and the mixed semi-finished product is stored in a closed temporary storage device. Wherein the temperature of the blending is preferably 30 ℃ and the relative humidity is preferably 40%.

Example 2

An infant formula milk powder with optimized milk fat formula is shown in table 2.

Table 2 ingredient formulation of example 2

The preparation method comprises the following steps:

(1) checking and accepting the raw milk and standardizing;

(2) mixing the mixed vegetable oil with the mixture of whey protein powder, yolk phospholipid and lactose according to the proportion of 1:3, emulsifying and homogenizing for 4 times, wherein the secondary homogenizing pressure is 5 MPa;

(3) uniformly mixing and completely dissolving raw milk, lactose, desalted whey powder, concentrated whey protein, mixed grease (soybean oil, coconut oil, palm oil, corn oil, 1.3 dioleate-2-palmitic acid triglyceride and 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride mixture) (the proportion is 6.4%: 14.6%: 34.4%: 5.6%: 39%), prebiotics, infant vitamin premix and infant mineral premix to obtain feed liquid B, homogenizing, sterilizing, concentrating and spray drying the obtained feed liquid B to obtain the material B;

(4) uniformly mixing the material B with docosahexaenoic acid (DHA) and arachidonic acid (ARA) powder at 25-30 ℃ and 40-50% of relative humidity during mixing;

(5) and packaging and storing by adopting a composite plastic container or an iron can.

Wherein, after the raw milk in the step (1) is accepted, the protein, fat and milk solid are strictly detected according to the technical requirements, and if the raw milk is not accepted, the raw milk is standardized. The raw milk comprises 2.95-3.10% of protein, 3.10-3.30% of fat and 11.7% of total solid.

Step (2), oil preparation: injecting the emulsified vegetable oil into the oiling cylinder, starting the stirrer, heating to 40-50 ℃, and preparing in the batching tank. Adding a small amount of water into the whey protein powder, the yolk phospholipid and the lactose, uniformly mixing, and pouring into a mixing tank to emulsify with the mixed oil. Wherein the ratio of the mixed grease to other raw material mixtures is 1: 3.

The homogenization in the step (3) is a conventional operation in the field, the homogenization pressure is preferably 15-25 MPa, and the temperature is 50-65 ℃. The sterilization is conventional operation in the field, such as pasteurization and ultra-high temperature sterilization, and preferably, the sterilization condition is that the temperature is 80-95 ℃ and the time is 30-40 seconds. The concentration is a conventional operation in the field, preferably a four-effect concentration process, and the solid concentration of the obtained concentrated milk is 40-50% (wt). The spray drying is a conventional operation in the field, preferably pressure spray drying, wherein the feeding temperature of the pressure spray drying is preferably 65-75 ℃, the air inlet temperature is preferably 150-190 ℃, the air exhaust temperature is preferably 68-85 ℃, and the pressure is preferably 150-300 bar.

Wherein the mixing method in step (4) is a conventional operation in the field. The mixing method is preferably a two-stage or three-stage dilution method, i.e., first, docosahexaenoic acid (DHA) and arachidonic acid (ARA) powders are premixed with the materials at a ratio of 1:10 by volume, and then, three-stage dry mixing is performed. Dry mixing and mixing: the dry mixing operation time is 2min, the rotating speed is 1460r/min, and the mixed semi-finished product is stored in a closed temporary storage device. Wherein the temperature of the blending is preferably 30 ℃ and the relative humidity is preferably 40%.

Comparative example 1

The raw material formulation (per 1000 g of milk powder) is shown in table 3.

Table 3 ingredient formulation of comparative example 1

The preparation method comprises the following steps:

(1) checking and accepting the raw milk and standardizing;

(2) mixing the mixed vegetable oil with the mixture of whey protein powder, yolk phospholipid and lactose according to the proportion of 1:3, emulsifying and homogenizing for 4 times, wherein the secondary homogenizing pressure is 5 MPa;

(3) uniformly mixing and completely dissolving raw milk, lactose, desalted whey powder, concentrated whey protein, mixed oil (soybean oil, peanut oil, corn oil, palm oil, high-oleic acid sunflower seed oil and coconut oil) (the proportion is respectively 24.2%: 6.39%: 0.63%: 39.59%: 1.7%: 27.49%), prebiotics, infant vitamin premix and infant mineral premix to obtain feed liquid B, homogenizing the obtained feed liquid B, sterilizing, concentrating and spray-drying to obtain the material B;

(4) uniformly mixing the material B with docosahexaenoic acid (DHA) and arachidonic acid (ARA) powder at 25-30 ℃ and 40-50% of relative humidity during mixing;

(5) and packaging and storing by adopting a composite plastic container or an iron can.

Wherein, after the raw milk in the step (1) is accepted, the protein, fat and milk solid are strictly detected according to the technical requirements, and if the raw milk is not accepted, the raw milk is standardized. The raw milk comprises 2.95-3.10% of protein, 3.10-3.30% of fat and 11.7% of total solid.

Wherein, the oil preparation in the step (2): injecting emulsified vegetable oil into oil tank, starting stirrer and raising

And (4) heating to 40-50 ℃, and preparing in a dosing tank. Adding a small amount of water into the whey protein powder, the yolk phospholipid and the lactose, uniformly mixing, and pouring into a mixing tank to emulsify with the mixed oil. Wherein the ratio of the mixed grease to other raw material mixtures is 1: 3.

Wherein the homogenization in the step (3) is a conventional operation in the field, the homogenization pressure is preferably 15-25 MPa, and the temperature is 50-65 ℃. The sterilization is conventional operation in the field, such as pasteurization and ultra-high temperature sterilization, and preferably, the sterilization condition is that the temperature is 80-95 ℃ and the time is 30-40 seconds. The concentration is a conventional operation in the field, preferably a four-effect concentration process, and the solid concentration of the obtained concentrated milk is 40-50% (wt). The spray drying is a conventional operation in the field, preferably pressure spray drying, wherein the feeding temperature of the pressure spray drying is preferably 65-75 ℃, the air inlet temperature is preferably 150-190 ℃, the air exhaust temperature is preferably 68-85 ℃, and the pressure is preferably 150-300 bar.

Wherein the mixing method in step (4) is a conventional operation in the field. The mixing method is preferably a two-stage or three-stage dilution method, i.e., first, docosahexaenoic acid (DHA) and arachidonic acid (ARA) powders are premixed with the materials at a ratio of 1:10 by volume, and then, three-stage dry mixing is performed. Dry mixing and mixing: the dry mixing operation time is 2min, the rotating speed is 1460r/min, and the mixed semi-finished product is stored in a closed temporary storage device. Wherein the temperature of the blending is preferably 30 ℃ and the relative humidity is preferably 40%.

Comparative example 2

The raw material formulation (per 1000 g of milk powder) is shown in table 4.

Table 4 ingredient formulation of comparative example 2

(1) The raw milk is accepted and standardized.

(2) Mixing the mixed vegetable oil with the mixture of whey protein powder, yolk phospholipid and lactose according to the proportion of 1:3, emulsifying and homogenizing, wherein the secondary homogenizing pressure is 5MPa, and homogenizing for 4 times.

(3) Uniformly mixing and completely dissolving raw milk, lactose, desalted whey powder, concentrated whey protein, mixed oil (soybean oil, corn oil, palm oil and coconut oil) in a ratio of 13.06%: 6.98%: 63.31%: 16.65%), prebiotics, infant vitamin premix and infant mineral premix to obtain feed liquid B, homogenizing the obtained feed liquid B, sterilizing, concentrating and spray drying to obtain the material B;

(4) uniformly mixing the material B with docosahexaenoic acid (DHA) and arachidonic acid (ARA) powder at 25-30 ℃ and 40-50% of relative humidity during mixing;

(5) and packaging and storing by adopting a composite plastic container or an iron can.

Wherein, after the raw milk in the step (1) is accepted, the protein, fat and milk solid are strictly detected according to the technical requirements, and if the raw milk is not accepted, the raw milk is standardized. The raw milk comprises 2.95-3.10% of protein, 3.10-3.30% of fat and 11.7% of total solid.

Wherein, the oil preparation in the step (2): injecting emulsified vegetable oil into oil tank, starting stirrer and raising

And (4) heating to 40-50 ℃, and preparing in a dosing tank. Adding a small amount of water into the whey protein powder, the yolk phospholipid and the lactose, uniformly mixing, and pouring into a mixing tank to emulsify with the mixed oil. Wherein the mixture ratio of the mixed grease to other raw material mixtures is 1: 3.

wherein the homogenization in the step (3) is a conventional operation in the field, the homogenization pressure is preferably 15-25 MPa, and the temperature is 50-65 ℃. The sterilization is conventional operation in the field, such as pasteurization and ultra-high temperature sterilization, and preferably, the sterilization condition is that the temperature is 80-95 ℃ and the time is 30-40 seconds. The concentration is a conventional operation in the field, preferably a four-effect concentration process, and the solid concentration of the obtained concentrated milk is 40-50% (wt). The spray drying is a conventional operation in the field, preferably pressure spray drying, wherein the feeding temperature of the pressure spray drying is preferably 65-75 ℃, the air inlet temperature is preferably 150-190 ℃, the air exhaust temperature is preferably 68-85 ℃, and the pressure is preferably 150-300 bar.

Wherein the mixing method in step (4) is a conventional operation in the field. The mixing method is preferably a two-stage or three-stage dilution method, i.e., first, docosahexaenoic acid (DHA) and arachidonic acid (ARA) powders are premixed with the materials at a ratio of 1:10 by volume, and then, three-stage dry mixing is performed. Dry mixing and mixing: the dry mixing operation time is 2min, the rotating speed is 1460r/min, and the mixed semi-finished product is stored in a closed temporary storage device. Wherein the temperature of the blending is preferably 30 ℃ and the relative humidity is preferably 40%.

Comparative example 3

The raw material formulation (per 1000 g of milk powder) is shown in table 5.

TABLE 5 ingredient formulation for comparative example 3

The formula of the infant formula as described above is prepared by a method comprising the steps of:

(1) checking and accepting the raw milk and standardizing;

(2) mixing the mixed vegetable oil with the mixture of whey protein powder, yolk phospholipid and lactose according to the proportion of 1:1, emulsifying and homogenizing, wherein the secondary homogenizing pressure is 5MPa, and homogenizing for 4 times.

(3) Uniformly mixing and completely dissolving raw milk, lactose, desalted whey powder, concentrated whey protein, mixed oil (29%: 17%: 11%: 4%: 39%) and fat (29%: 17%: 11%: 4%: 39%) respectively), prebiotics, infant vitamin premix and infant mineral premix to obtain feed liquid B, homogenizing the obtained feed liquid B, sterilizing, concentrating and spray-drying to obtain the material B;

(4) uniformly mixing the material B with docosahexaenoic acid (DHA) and arachidonic acid (ARA) powder at 25-30 ℃ and 40-50% of relative humidity during mixing;

(5) and packaging and storing by adopting a composite plastic container or an iron can.

Wherein, after the raw milk in the step (1) is accepted, the protein, fat and milk solid are strictly detected according to the technical requirements, and if the raw milk is not accepted, the raw milk is standardized. The raw milk comprises 2.95-3.10% of protein, 3.10-3.30% of fat and 11.7% of total solid.

Wherein, the oil preparation in the step (2): injecting emulsified vegetable oil into oil tank, starting stirrer and raising

And (4) heating to 40-50 ℃, and preparing in a dosing tank. Adding a small amount of water into the whey protein powder, the yolk phospholipid and the lactose, uniformly mixing, and pouring into a mixing tank to emulsify with the mixed oil. Wherein the ratio of the mixed grease to other raw material mixtures is 1: 1.

Wherein the homogenizing pressure in the step (3) is preferably 15-25 MPa, and the temperature is 50-65 ℃. The sterilization is conventional operation in the field, such as pasteurization and ultra-high temperature sterilization, and preferably, the sterilization condition is that the temperature is 80-95 ℃ and the time is 30-40 seconds. The concentration is a conventional operation in the field, preferably a four-effect concentration process, and the solid concentration of the obtained concentrated milk is 40-50% (wt). The spray drying is a conventional operation in the field, preferably pressure spray drying, wherein the feeding temperature of the pressure spray drying is preferably 65-75 ℃, the air inlet temperature is preferably 150-190 ℃, the air exhaust temperature is preferably 68-85 ℃, and the pressure is preferably 150-300 bar.

Wherein the mixing method in step (4) is a conventional operation in the field. The mixing method is preferably a two-stage or three-stage dilution method, i.e., first, docosahexaenoic acid (DHA) and arachidonic acid (ARA) powders are premixed with the materials at a ratio of 1:10 by volume, and then, three-stage dry mixing is performed. Dry mixing and mixing: the dry mixing operation time is 2min, the rotating speed is 1460r/min, and the mixed semi-finished product is stored in a closed temporary storage device. Wherein the temperature of the blending is preferably 30 ℃ and the relative humidity is preferably 40%.

Comparative example 4

The raw material formulation (per 1000 g of milk powder) is shown in table 6.

TABLE 6 ingredient formulation for comparative example 4

(1) Checking and accepting the raw milk and standardizing;

(2) mixing the mixed vegetable oil with the mixture of whey protein powder, yolk phospholipid and lactose according to the proportion of 1:1, emulsifying and homogenizing for 4 times, wherein the secondary homogenizing pressure is 5 MPa;

(3) uniformly mixing and completely dissolving raw milk, lactose, desalted whey powder, concentrated whey protein, mixed grease (soybean oil, coconut oil, palm oil, corn oil, 1.3 dioleate-2-palmitic acid triglyceride and 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride mixture) (the proportion is 6.4%: 14.6%: 34.4%: 5.6%: 39%), prebiotics, infant vitamin premix and infant mineral premix to obtain feed liquid B, homogenizing, sterilizing, concentrating and spray drying the obtained feed liquid B to obtain the material B;

(4) uniformly mixing the material B with docosahexaenoic acid (DHA) and arachidonic acid (ARA) powder at 25-30 ℃ and 40-50% of relative humidity during mixing;

(5) and packaging and storing by adopting a composite plastic container or an iron can.

Wherein, after the raw milk in the step (1) is accepted, the protein, fat and milk solid are strictly detected according to the technical requirements, and if the raw milk is not accepted, the raw milk is standardized. The raw milk comprises 2.95-3.10% of protein, 3.10-3.30% of fat and 11.7% of total solid.

Wherein, the oil preparation in the step (2): injecting the emulsified vegetable oil into the oiling cylinder, starting the stirrer, heating to 40-50 ℃, and preparing in the batching tank. Adding a small amount of water into the whey protein powder, the yolk phospholipid and the lactose, uniformly mixing, and pouring into a mixing tank to emulsify with the mixed oil. Wherein the ratio of the mixed grease to other raw material mixtures is 1: 1.

Wherein the homogenization in the step (3) is a conventional operation in the field, the homogenization pressure is preferably 15-25 MPa, and the temperature is 50-65 ℃. The sterilization is conventional operation in the field, such as pasteurization and ultra-high temperature sterilization, and preferably, the sterilization condition is that the temperature is 80-95 ℃ and the time is 30-40 seconds. The concentration is a conventional operation in the field, preferably a four-effect concentration process, and the solid concentration of the obtained concentrated milk is 40-50% (wt). The spray drying is a conventional operation in the field, preferably pressure spray drying, wherein the feeding temperature of the pressure spray drying is preferably 65-75 ℃, the air inlet temperature is preferably 150-190 ℃, the air exhaust temperature is preferably 68-85 ℃, and the pressure is preferably 150-300 bar.

Wherein the mixing method in step (4) is a conventional operation in the field. The mixing method is preferably a two-stage or three-stage dilution method, i.e., first, docosahexaenoic acid (DHA) and arachidonic acid (ARA) powders are premixed with the materials at a ratio of 1:10 by volume, and then, three-stage dry mixing is performed. Dry mixing and mixing: the dry mixing operation time is 2min, the rotating speed is 1460r/min, and the mixed semi-finished product is stored in a closed temporary storage device. Wherein the temperature of the blending is preferably 30 ℃ and the relative humidity is preferably 40%.

Effects of the embodiment

The product evaluations were conducted for examples 1 to 2 and comparative examples 1 to 4.

The evaluation method is to measure and determine the main total fatty acid composition (%) of the substitute fat of the mixed vegetable oil and to perform detection analysis by a gas chromatograph Agilent 7820A. The main triglyceride composition (%) of the substitute fat of the mixed vegetable oil was subjected to reverse phase high performance liquid chromatography (RP-HPLC). The evaluation results are shown in tables 7 and 8.

Table 7 main triglyceride composition of alternative fats of mixed vegetable oils (%)

Fatty acids	Example 1	Example 2	Comparative example 1	Comparative example 2	Breast milk
						C10：0	1.21	1.58	0.83	0.96	1.13
C12：0	7.25	6.75	12.88	7.8	4.26
						C14：0	4.10	4.08	5.51	5.44	4.02
C16：0	23.78	22.49	25.01	26.63	22.4
						C16：1	1.27	1.47	0.03	0.77	1.87
C18：0	4.68	4.87	3.67	6.42	5.96
						C18：1	38.71	31.23	26.04	31.65	32.16
C18：2	17.52	21.35	21.23	17.61	23.44
						C10：0	1.21	1.58	0.83	0.96	1.13

Table 8 main triglyceride composition of alternative fats of mixed vegetable oils (%)

Physical and chemical properties of 9 prepared fat milk samples of example 1, example 2, comparative example 1, comparative example 2, comparative example 3 and comparative example 4 were measured by a PSS particle size analyzer. The measurement indexes include an average particle diameter, a particle diameter distribution, a D90 value, and a zeta potential. The measurement results are shown in Table 9.

TABLE 9 comparison of results of different proportions of emulsifiability

The determination result shows that the mixed vegetable oil, the whey protein powder, the egg yolk phospholipid and the lactose are mixed according to the proportion of 3: the emulsifying effect is better when the proportion of 1 is used. 1: the ratio of 1 is poor in emulsifying effect. The average particle size is relatively large; the particle size distribution is relatively wide; the corresponding value of D90 is also relatively large; whereas the zeta potential differs less.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.