阅读说明：本技术 一种金黄色海水珍珠的培育方法 (Golden yellow seawater pearl cultivation method ) 是由 何德边 于 2020-12-31 设计创作，主要内容包括：本发明公开一种金黄色海水珍珠的培育方法,包括步骤：S1、挑选贝壳内侧层为黄色的珍珠贝并获取外套膜细胞小片；S2、挑选贝壳内侧层为黄色的珠母贝并手术植入珠核,再将前述获得的细胞小片贴附在珠核上；S3、将手术后的珠母贝放入海水池塘中休养25-35天；去除死贝并清理活贝的附着物后,继续养殖；S4、养殖2-3个月后,去除死贝并清理附着物后继续养殖；重复4-5次,完成培育过程。本发明通过挑选贝壳内侧表层为黄色的珍珠贝上获取外套膜细胞小片,进行手术通过细胞移植连同珠核一同植入到贝壳内侧层为黄色的珠母贝的核位内,在养殖过程中,通过珠母贝内分泌分解融化形成珍珠囊,形成金黄色的珍珠质层,从而产出金黄色珍珠。(The invention discloses a cultivation method of golden yellow seawater pearls, which comprises the following steps: s1, selecting yellow pearl oyster on the inner layer of the shell and obtaining a mantle cell patch; s2, selecting yellow nacre as the inner layer of the shell, implanting the nacre into the nacre through an operation, and attaching the obtained cell sheet to the nacre; s3, putting the operated nacre into a seawater pond for resting for 25-35 days; removing dead shellfish and cleaning attachments of live shellfish, and continuously culturing; s4, after 2-3 months of cultivation, removing dead shellfish and cleaning attachments for continuous cultivation; repeating for 4-5 times to complete the cultivation process. The method comprises the steps of selecting yellow pearl shells on the inner surface layers of shells to obtain mantle cell pieces, carrying out operation, transplanting the pearl cell pieces and pearl nuclei into nucleus positions of the yellow pearl shells on the inner surface layers of the shells together through cell transplantation, and carrying out endocrine decomposition and melting on the pearl shells to form pearl sacs and form golden pearl shells in the culture process, so that golden pearls are produced.)

1. A cultivation method of golden yellow seawater pearls is characterized in that: the method comprises the following steps:

s1, selecting yellow pearl oyster with the shell inner layer, obtaining connective tissues in the center of the mantle of the pearl oyster, and cutting to obtain small cell pieces;

s2, selecting a healthy mature nacre with yellow inner layer, implanting a pearl nucleus into the pearl nucleus site through surgery, and sending the cell slice obtained in the step S1 into the nucleus site and attaching the cell slice to the pearl nucleus;

s3, putting a plurality of operated nacre into a group, and putting the nacre into a seawater pond for resting for 25-35 days; after the recuperation is finished, removing dead shellfish and cleaning attachments on the nacre, and continuously culturing according to a conventional method;

s4, after 2-3 months of cultivation, removing dead shellfish, cleaning attachments on the nacre, and continuing cultivation;

s5, executing the step S4, repeating for 4-5 times, and finishing the cultivation process.

2. The cultivation method of golden yellow seawater pearls as claimed in claim 1, wherein: in the step S3, after the pearl oyster is completely incubated, the pearl oyster with the attachments cleaned is firstly identified and screened by an X-ray machine, and then the pearl oyster with the nuclei is continuously incubated.

3. A cultivation method of golden yellow seawater pearls according to claim 1 or 2, characterized in that: the method is characterized in that the nacre is recuperated before a nucleus implanting operation, and the method comprises the following specific operations: and (5) hanging and culturing the nacre selected in the step (S2), doubling the number of the nacre in each hanging and culturing cage compared with the number of the nacre in the normal hanging and culturing, and continuing culturing for 8-12 days.

4. The cultivation method of golden yellow seawater pearls as claimed in claim 3, wherein: the water temperature of the culture seawater which is recuperated before the operation is kept at 16-27 ℃, and the dissolved oxygen is 6-10 mg/L.

Technical Field

The invention belongs to the technical field of seawater pearl cultivation, and particularly relates to a cultivation method of golden yellow seawater pearls.

Background

Pearls are precious jewelry that is biomineralized in molluscs and generally classified into seawater pearls and freshwater pearls. Compared with fresh water pearls, most seawater pearls have high roundness, strong luster and more delicate hand feeling, and have more appreciation and medicinal values. In the prior art, seawater pearl cultivation is mainly performed by nucleus insertion cultivation, pearl nuclei are implanted into shells such as Pinctada martensii dunker through an operation and then placed into a suitable cultivation environment, and pearl nuclei are wrapped by a pearl layer secreted by the Pinctada martensii dunker through natural cultivation to form pearls. At present, because the color of the pearl is closely related to the color of the shell of the pearl donor, the pearl produced by the Margaritifera pearl oyster through mariculture has single color, only one color is on a single bead, and the appearance is monotonous. In order to obtain multicolor effect commercially, the pearl nucleus dyeing or finished pearl dyeing can only be carried out, and the artificial color-adding effect is always achieved, so that the pursuit of people cannot be met.

Disclosure of Invention

The invention aims to provide a method for cultivating golden seawater pearls, which can cultivate golden seawater pearls.

In order to achieve the purpose, the invention provides the following technical scheme: a cultivation method of golden yellow seawater pearls is characterized in that: the method comprises the following steps: s1, selecting yellow pearl oyster with the shell inner layer, obtaining connective tissues in the center of the mantle of the pearl oyster, and cutting to obtain small cell pieces; s2, selecting a healthy mature nacre with yellow inner layer, implanting a pearl nucleus into the pearl nucleus site through surgery, and sending the cell slice obtained in the step S1 into the nucleus site and attaching the cell slice to the pearl nucleus; s3, placing a plurality of Pinctada martensii shells implanted with pearl nuclei and cell slices into a seawater pond for resting for 25-35 days; after the recuperation is finished, removing dead shellfish and cleaning attachments on the nacre, and continuously culturing according to a conventional method; s4, after 2-3 months of cultivation, removing dead shellfish and cleaning attachments on the nacre; s5, executing the step S4, repeating for 4-5 times, and finishing the cultivation process.

Further, in the step S3, after the pearl oyster is completely incubated, the pearl oyster with the attachments cleaned is firstly identified and screened by an X-ray machine, and then the pearl oyster with the nucleus is continuously cultured.

Further, the nacre is recuperated before the nucleus implanting operation, and the specific operation is as follows: and (5) hanging and culturing the nacre selected in the step (S2), doubling the number of the nacre in each hanging and culturing cage compared with the number of the nacre in the normal hanging and culturing, and continuing culturing for 8-12 days. Specifically, the water temperature of the culture seawater which is recuperated before the operation is kept at 16-27 ℃, and the dissolved oxygen is 6-10 mg/L.

Compared with the prior art, the invention has the following beneficial effects: by adopting the cultivation method, mantle cell pieces are obtained from the pearl shells with yellow inner surface layers of the shells, the pearl shells are transplanted into nucleus positions of the yellow pearl shells with yellow inner surface layers of the shells through cell transplantation and pearl nucleus transplantation, and during cultivation, the pearl shells are subjected to endocrine decomposition and melting to form pearl sacs and form golden pearl shells, so that golden pearls are produced.

Whether the nucleuses are left after the recuperation is further distinguished through an X-ray machine, the seedless nacre can be re-seeded and then cultured continuously, the pearl production rate is improved, and the culture cost is reduced.

Drawings

FIG. 1 is a flow chart of the cultivation method of the present invention.

Detailed Description

In order to make those skilled in the art understand the technical solutions of the present invention well, the following description of the present invention is provided with reference to the embodiments and the accompanying drawings, but the embodiments of the present invention are not limited thereto.

As shown in figure 1, the cultivation method of the golden yellow seawater pearl comprises the following steps.

S1, selecting yellow pearl oyster with the shell inner layer, obtaining connective tissues in the center of the mantle of the pearl oyster, and cutting to obtain small cell pieces; and subjecting the cell pellet to a sterilization treatment.

S2, selecting a healthy mature nacre with yellow inner layer, implanting a pearl nucleus into the pearl nucleus site through surgery, and sending the cell slice obtained in the step S1 into the nucleus site and attaching the cell slice to the pearl nucleus; ensuring that the cell sheet and the pearl nucleus are together resident in the nuclear site.

S3, putting a plurality of operated nacre into a group, and putting the nacre into a seawater pond for resting for 25-35 days; the specific rest time can be set according to the actual situation as long as the rest time is within the interval, such as 28 days and 30 days. And after the rest cultivation is finished, removing dead shellfish, cleaning attachments on the nacre, and continuously cultivating according to a conventional method.

And S4, after 2-3 months of cultivation, removing dead shellfish, cleaning attachments on the nacre, and continuing cultivation.

And S5, executing the step S4, repeating the step 4 or 5 times, and finishing the cultivation process.

Preferably, some pearl nuclei are spitted out in the cultivation process after the nucleus planting, and if the cultivation is carried out again, pearls are not produced, so that the cultivation cost is increased indirectly. In contrast, after the nacre is retired, the nacre with the attachments cleaned is firstly screened out by an X-ray machine, and then the nacre with the cores is continuously cultured. Through the steps, whether the nucleuses are reserved in the nucleuses of the nacre can be identified through an X-ray machine, and the nacre-producing body without the nucleuses can be found in time. Thus, the pearl producing shell bodies without the preserved pearl nuclei can be removed after the rest period, and the subsequent hanging-cultured pearl producing shell bodies are ensured to contain nuclei; the pearl yield of the cultivation is improved; the pearl producing shell body without the pearl nucleus can be cultured and planted with the pearl nucleus again; effectively reducing the breeding cost.

In addition, the nacre (pearl producing shell) can be recuperated before the nucleus planting operation, so that the success rate of the nucleus planting operation can be increased. The specific operation is as follows: and (5) putting the nacre selected in the step (S2) into a seawater pond for suspension culture, doubling the number of the nacre in each suspension culture cage compared with the number of the nacre in the normal suspension culture, and continuously culturing for 8-12 days. Wherein the water temperature of the culture seawater which is recuperated before the operation is kept at 16-27 ℃, and the dissolved oxygen is 6-10 mg/L. After the pearl oyster is recuperated before an operation, the physiological growth of the pearl oyster is inhibited, the gonad of the pearl oyster is not too full, the nucleus planting effect is good, the pearl oyster can be quickly recovered after the nucleus planting, the death rate after the nucleus planting is reduced, and the nucleus planting success rate is improved.

The present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents and are included in the scope of the present invention.