阅读说明：本技术 一种无核海水珍珠的培育方法 (Cultivation method of seedless seawater pearls ) 是由 何德边 于 2020-12-31 设计创作，主要内容包括：本发明公开一种无核海水珍珠的培育方法,包括如下步骤：S1、挑选健康成熟的马氏珍珠贝,获取外套膜中央的结缔组织,切割获得多个外套膜细胞小片,每个细胞小片的长为2-3mm,宽为1-2mm；S2、另外挑选健康成熟的马氏珍珠贝作为产珠贝体,往产珠贝体的核位中手术植入多个步骤S1中获得的细胞小片；S3、将手术后的产珠贝体放入海水池塘中休养25-35天；去除死贝并清理珠母贝上的附着物,按常规方法继续养殖；S4、养殖2-3个月后,去除死贝并清理附着物后继续养殖；重复4-5次,完成培育过程。采用本发明的培育方法,通过将处理后的多片外套膜细胞小片植入产珠马氏珍珠贝的核位内,经过养殖后获得无核的海水珍珠。(The invention discloses a cultivation method of a seedless seawater pearl, which comprises the following steps: s1, selecting healthy and mature Pinctada martensii, obtaining connective tissues in the center of a mantle, cutting to obtain a plurality of mantle cell slices, wherein each cell slice is 2-3mm long and 1-2mm wide; s2, selecting healthy and mature Pinctada martensii as a Pinctada martensii body, and surgically implanting a plurality of cell fragments obtained in the step S1 into the nucleus site of the Pinctada martensii body; s3, putting the operated pearl producing shell bodies into a seawater pond for resting for 25-35 days; removing dead shellfish and cleaning attachments on the nacre, and continuously culturing according to a conventional method; s4, after 2-3 months of cultivation, removing dead shellfish and cleaning attachments for continuous cultivation; repeating for 4-5 times to complete the cultivation process. By adopting the cultivation method, the treated plurality of mantle cell pieces are implanted into the nucleus positions of the pearl producing Pinctada martensii dunker, and the seedless seawater pearls are obtained after cultivation.)

1. A cultivation method of a non-nucleus seawater pearl is characterized in that: the method comprises the following steps:

s1, selecting healthy and mature Pinctada martensii, obtaining connective tissues in the center of a mantle, cutting to obtain a plurality of mantle cell slices, wherein each cell slice is 2-3mm long and 1-2mm wide;

s2, selecting healthy and mature Pinctada martensii as a Pinctada martensii body, and surgically implanting a plurality of cell fragments obtained in the step S1 into the nucleus site of the Pinctada martensii body;

s3, putting a plurality of post-operation pearl producing shells into a group, and putting the groups into a seawater pond for recuperation for 25-35 days; removing dead shellfish after rest, cleaning attachments on the shells of the live shellfish, regrouping and putting into seawater for continuous culture according to a conventional method;

s4, culturing for 2-3 months, removing dead shellfish, cleaning attachments on the shells of live shellfish, and continuously culturing;

s5, executing the step S4, repeating for 5-7 times, and finishing the cultivation process.

2. The cultivation method of the nucleus-free seawater pearl as claimed in claim 1, wherein: in step S4, after the attachments of the live shellfish are cleaned for the first time, the live shellfish is passed through an X-ray machine to identify the pearl producing bodies that have produced the pearl sacs, and then the cultivation is continued.

3. The cultivation method of the nucleus-free seawater pearl as claimed in claim 1, wherein: the method is characterized in that the pearl producing shell body is firstly recuperated before a nucleus implanting operation, and the specific operation is as follows: hanging culturing the second Pinctada martensii dunker selected in the step S2, doubling the number of Pinctada martensii dunker in each hanging culturing cage compared with the number of Pinctada martensii dunker in normal hanging culturing, and continuously culturing for 8-12 days; wherein the water temperature of the culture seawater in the seawater pond is kept at 16-27 ℃, and the dissolved oxygen is 6-10 mg/L.

4. A cultivation method of a nucleus-free seawater pearl according to any one of claims 1 to 3, characterized in that: the number of implanted cell pieces in the step 2 is 3-5.

Technical Field

The invention belongs to the technical field of seawater pearl cultivation, and particularly relates to a cultivation method of a seedless seawater pearl.

Background

Pearls are precious jewelry that is biomineralized in molluscs and generally classified into seawater pearls and freshwater pearls. Compared with fresh water pearls, most seawater pearls have high roundness, strong luster and more delicate hand feeling, and have more appreciation and medicinal values. In the prior art, seawater pearl cultivation is mainly performed by nucleus planting cultivation, pearl nuclei are planted in shells such as Pinctada martensii dunker through an operation, then the shells are placed in a proper cultivation environment, and pearls are formed by wrapping the pearl nuclei by a pearl layer secreted by the Pinctada martensii dunker through natural cultivation. Most of pearls cultured by nucleus planting are pearl nuclei, only the outer pearl substance layer has important value, and in the separation process of nacre and the pearl nuclei, the nacre part with medicinal value can be mixed with a plurality of material components of the pearl nuclei, so that the medicinal value is greatly reduced. In the prior art, a lot of researches are carried out on the cultivation of the seedless seawater pearls, and some researches can also produce the seedless seawater pearls, but the operation process is very complicated and the cultivation cost is high.

Disclosure of Invention

The invention aims to provide a cultivation method of the seedless seawater pearls, which is simple to operate and low in cultivation cost.

In order to achieve the purpose, the invention provides the following technical scheme: a cultivation method of a non-nucleus seawater pearl is characterized in that: the method comprises the following steps: s1, selecting healthy and mature Pinctada martensii, obtaining connective tissues in the center of a mantle, cutting to obtain a plurality of mantle cell slices, wherein each cell slice is 2-3mm long and 1-2mm wide; s2, selecting healthy and mature Pinctada martensii as a Pinctada martensii body, and surgically implanting a plurality of cell fragments obtained in the step S1 into the nucleus site of the Pinctada martensii body; s3, putting a plurality of post-operation pearl producing shells into a group, and putting the groups into a seawater pond for recuperation for 25-35 days; removing dead shellfish after rest, cleaning attachments on the shells of the live shellfish, regrouping and putting into seawater for continuous culture according to a conventional method; s4, culturing for 2-3 months, removing dead shellfish, cleaning attachments on the shells of live shellfish, and continuously culturing; s5, executing the step S4, repeating for 5-7 times, and finishing the cultivation process.

Furthermore, after the pearl producing shell is retired, the pearl producing shell with pearl sacs produced can be distinguished by the pearl producing shell with the attachments cleaned and cultured continuously through an X-ray machine.

Further, the pearl producing shell is recuperated before the operation of nuclear implantation, and the specific operation is as follows: and (5) hanging and culturing the second Pinctada martensii dunker selected in the step (S2), doubling the number of Pinctada martensii dunker in each hanging and culturing cage compared with the number of Pinctada martensii dunker in normal hanging and culturing for 8-12 days. Wherein the water temperature of the culture seawater in the seawater pond is kept at 16-27 ℃, and the dissolved oxygen is 6-10 mg/L.

Further, the number of the implanted cell pieces in the step 2 is 3-5.

Compared with the prior art, the invention has the following beneficial effects: by adopting the cultivation method, the treated plurality of mantle cell pieces are implanted into the nucleus positions of the pearl producing Pinctada martensii dunker, in the cultivation process, pearl sacs are formed in the nucleus positions of the Pinctada martensii dunker, the nacre is secreted, and finally, the seawater pearls without nuclei are obtained; can be used as raw material of medicine or cosmetics.

Drawings

FIG. 1 is a flow chart of the cultivation method of the present invention.

Detailed Description

In order to make those skilled in the art understand the technical solutions of the present invention well, the following description of the present invention is provided with reference to the embodiments and the accompanying drawings, but the embodiments of the present invention are not limited thereto.

As shown in figure 1, the cultivation method of the non-nucleated seawater pearl of the invention comprises the following steps:

s1, selecting healthy and mature Pinctada martensii, obtaining connective tissues in the center of the mantle, cutting to obtain a plurality of mantle cell slices, and sterilizing the obtained cell slices, wherein the length of each cell slice is 2-3mm, and the width of each cell slice is 1-2 mm.

S2, selecting healthy and mature Pinctada martensii as a Pinctada martensii body, and surgically implanting 3-5 cell slices obtained in the step S1 into the nucleus site of the Pinctada martensii body.

S3, putting a plurality of post-operation pearl producing shells into a group, and putting the groups into a seawater pond for recuperation for 25-35 days; removing dead shellfish after rest, cleaning attachments on the shells of live shellfish, grouping again, and putting into seawater for continuous culture according to conventional method.

S4, culturing for 2-3 months, removing dead shellfish, cleaning attachments on the shells of live shellfish, and continuing culturing.

S5, executing the step S4, repeating for 5-7 times, and finishing the cultivation process.

Preferably, since the mantle cell pieces may be spitted out or decomposed during the cultivation process after implantation, pearls cannot be produced any more, and cultivation cost is increased if the cultivation is continued according to the conventional cultivation method. In contrast, after the pearl shell of the Pinctada martensii is cultured for 2-3 months, the attachments on the shell of the Pinctada martensii are cleaned, the pearl shell producing bodies are firstly made to pass through an X-ray machine, so that the Pinctada martensii with the formed pearl sacs are distinguished, and then the Pinctada martensii is cultured. By this procedure, a pearl producing shell body in which a pearl sac is not formed can be found. Thus, the pearl producing shell bodies without pearl sacs can be removed after the conventional culture in the first stage, and the pearl producing shell bodies cultured in a subsequent hanging way can produce pearls; the pearl yield of the cultivation is improved; the pearl-producing shell body without the pearl sac can be reused; effectively reducing the breeding cost.

In addition, the pearl shell producing body (Pinctada martensii) can be recuperated before the cell patch implantation operation, so that the success rate of the nucleus implantation operation can be increased. The specific operation is as follows: putting the second Pinctada martensii dunker selected in the step S2 into a seawater pond for suspension culture, doubling the number of Pinctada martensii dunker in each suspension culture cage compared with the number of Pinctada martensii dunker in normal suspension culture, and continuously culturing for 8-12 days; wherein the water temperature of the culture seawater in the seawater pond is kept at 16-27 ℃, and the dissolved oxygen is 6-10 mg/L. After preoperative recuperation, the physical growth of the Pinctada martensii dunker is inhibited, the gonad of the shellfish is not excessively full, the nucleus planting effect is good, the shellfish can be quickly recovered after nucleus planting, the death rate after nucleus planting is reduced, and the nucleus planting success rate is improved.

The present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents and are included in the scope of the present invention.