阅读说明：本技术 一种应用茶翅蝽诱剂协同剂的引诱剂组合物及其应用和诱芯 (Attractant composition applying tea-wing stinkbug attractant synergist and application and lure thereof ) 是由 唐睿 钟永志 韩日畴 曹莉 陈浩梁 于 2020-12-11 设计创作，主要内容包括：本发明公开了一种应用茶翅蝽诱剂协同剂的引诱剂组合物及其应用和诱芯。所述的茶翅蝽诱剂组合物包括茶翅蝽聚集信息素和协同剂,所述的茶翅蝽聚集信息素和协同剂的重量比为0.25～4:1。所述的协同剂选自苯甲醛、芳樟醇、氧化芳樟醇中的三种做不同配比。本发明提供的诱剂组合物可以制成诱芯,以配合已有的茶翅蝽聚集信息素使用。本发明提供含有茶翅蝽诱剂组合物成分的诱芯,用以防治和检测茶翅蝽的发生。本发明解决了现有技术中进口和国产茶翅蝽聚集信息素诱芯成本高、难普及的缺点,成本低廉、使用灵活,而且诱捕效率更高。(The invention discloses an attractant composition applying a tea bug attractant synergist, and application and a lure thereof. The tea bug attractant composition comprises a tea bug gathering pheromone and a synergist, wherein the weight ratio of the tea bug gathering pheromone to the synergist is 0.25-4: 1. The synergist is selected from three of benzaldehyde, linalool and linalool oxide in different proportions. The attractant composition provided by the invention can be prepared into an attracting core to be matched with the existing gathering pheromone of the tea bug. The invention provides a lure containing components of a tea bug attractant composition, which is used for preventing and detecting the occurrence of tea bug. The invention overcomes the defects of high cost and difficult popularization of imported and domestic tea bug gathering pheromone lure in the prior art, and has the advantages of low cost, flexible use and higher trapping efficiency.)

1. An attractant composition applying a tea bug attractant synergist is characterized by comprising the synergist of the tea bug attractant and tea bug aggregation pheromone, wherein the weight ratio of the synergist to the tea bug aggregation pheromone is 1: 0.25-4; the synergist comprises the following components in parts by weight: 5-8 parts of linalool oxide, 2-5 parts of linalool, 1-2 parts of benzaldehyde and 0.5-2 parts of ethyl lactate.

2. The attractant composition applying the tea bug attractant synergist according to claim 1, wherein the weight ratio of the synergist to the tea bug aggregation pheromone is 1: 1-4.

3. The attractant composition applying the tea bug attractant synergist according to claim 1, wherein the synergist comprises the following components in parts by weight: linalool oxide 6.5 parts, linalool 2.5 parts, benzaldehyde 1 part and ethyl lactate 0.5 part.

4. The synergist of the tea bug attractant is characterized by comprising the following components in parts by weight: 5-8 parts of linalool oxide, 2-5 parts of linalool, 1-2 parts of benzaldehyde and 0.5-2 parts of ethyl lactate.

5. The synergist of the tea wing bug attractant as claimed in claim 4, wherein the synergist comprises the following components in parts by weight: linalool oxide 6.5 parts, linalool 2.5 parts, benzaldehyde 1 part and ethyl lactate 0.5 part.

6. Use of an attractant composition for use of a tea bug attractant synergist as claimed in any one of claims 1 to 3 in the control and/or monitoring of tea bug.

7. The use of claim 6, comprising applying the synergist to a lure containing a tea bug aggregation pheromone; or the application comprises that the synergist is mixed with the tea bug aggregation pheromone and then is applied to a blank lure core; or the application comprises applying the synergist to a blank lure and matching the lure containing the synergist with the gathering pheromone of the tea bug.

8. A lure of a tea bug attractant, which is characterized by containing the attractant composition applying the tea bug attractant synergist in any one of claims 1-3.

9. A method of controlling and/or monitoring a tea bug, comprising applying an attractant composition for applying a tea bug attractant synergist according to any one of claims 1 to 3 to the habitat of a tea bug.

Technical Field

The invention relates to the technical field of pest monitoring and trapping, in particular to an attractant composition applying a tea bug attractant synergist, application thereof and a lure.

Background

Tea-wing bugs (halomorpha haiys) are a europaous pest of origin in east asia and have become an important worldwide agricultural and forestry pest due to their extensive host plants and their invasion into several countries in north america, europe and south america, and africa. The adult and nymph tea-wing bugs are mainly fed in a manner that the piercing-sucking mouthparts are pierced into plants and fruits to suck nutrient substances, the pierced fruits can generate insect spots and can cause lignification inside the fruits to be abnormal fruits which cannot be eaten, the fruit grade is seriously reduced, the economic benefit of the fruits is greatly reduced, and the tender tips of the pierced plants cannot be continuously developed. The tea-wing bugs did not become major pests in the 60-70 s, but by the 80 s their population number increased dramatically due to changes in planting and farming conditions and were listed as one of the major pests on fruit trees.

The occurrence of the tea bug can be timely and accurately forecasted by utilizing an aggregation pheromone monitoring means, and effective measures can be timely taken for prevention and treatment; the increase of the number of the tea plant bugs can be controlled by utilizing a large-scale trapping and killing technology of the gathering pheromone, and the pollution to the environment is avoided.

The existing imported lure taking aggregation pheromone as a raw material has the defects of high cost and difficulty in popularization, mainly comprises two components, and the two components of the imported lure of the tea bug are as follows: (3S,6S,7R,10S) -10,11-epoxy-1-bisabolen-3-ol and (3R,6S,7R,10S) -10, 11-epoxy-1-bisabolen-3-ol. For example, the imported tea-wing stink bugs gather pheromone lure of China agricultural academy of sciences and northwest agriculture and forestry university is used for monitoring the occurrence of the tea-wing stink bugs in Yangling, Yanyang and surrounding areas, and the lure has certain trapping activity, but is expensive and difficult to popularize and use on a large scale. The domestic 4-component self-made lure is relatively low in cost, but the trapping effect is not ideal, and due to low persistence, the use and popularization cost is increased due to frequent replacement.

Therefore, the lure containing the tea bug aggregation pheromone and the synergist composition thereof needs to be provided to relieve the problems of high cost and difficult popularization, and meanwhile, diversified choices are provided for the monitoring technology of the tea bug.

Disclosure of Invention

The invention aims to overcome the defects of high cost and difficult popularization of the gathering pheromone of the tea bug in the prior art, and provides a synergistic agent and a composition of the luring agent of the tea bug gathering pheromone, application and a lure of the luring agent composition so as to reduce the using amount of the gathering pheromone and improve the gathering activity, thereby reducing the cost. The invention also aims to provide application of the attractant composition in controlling and/or monitoring tea plant bugs and an attractant core containing the attractant composition.

The first purpose of the invention is to provide an attractant composition applying a tea bug attractant synergist, the attractant composition comprises the synergist of the tea bug attractant and a tea bug aggregation pheromone, and the weight ratio of the synergist to the tea bug aggregation pheromone is 1: 0.25-4; the synergist comprises the following components in parts by weight: 5-8 parts of linalool oxide, 2-5 parts of linalool, 1-2 parts of benzaldehyde and 0.5-2 parts of ethyl lactate.

Preferably, the weight ratio of the synergist to the tea bug aggregation pheromone is 1: 1-4.

The invention aims to provide a synergist of a tea bug attractant, which comprises the following components in parts by weight: 5-8 parts of linalool oxide, 2-5 parts of linalool, 1-2 parts of benzaldehyde and 0.5-2 parts of ethyl lactate.

Preferably, the synergist comprises the following components in parts by weight: linalool oxide 6.5 parts, linalool 2.5 parts, benzaldehyde 1 part and ethyl lactate 0.5 part.

The third purpose of the invention is to provide the application of the attractant composition applying the tea bug attractant synergist in controlling and/or monitoring the tea bug.

Preferably, the application comprises applying the synergist to a lure containing an orius ferus gathering pheromone; or the application comprises that the synergist is mixed with the tea bug aggregation pheromone and then is applied to a blank lure core; or the application comprises applying the synergist to a blank lure and matching the lure containing the synergist with the gathering pheromone of the tea bug.

The fourth purpose of the invention is to provide a lure of the tea bug attractant, which contains the attractant composition applying the tea bug attractant synergist.

A fifth object of the present invention is to provide a method of controlling and/or monitoring a tea bug comprising applying an attractant composition as described above applying a tea bug attractant synergist to the habitat of a tea bug.

Preferably, the use amount of the attractant composition applying the tea bug attractant synergist is 0.0015-0.15 mg/square meter.

Compared with the prior art, the invention has the advantages that:

(1) the use mode is flexible. The tea-wing stinkbug attractant composition provided by the invention comprises tea-wing stinkbug gathering pheromone and a synergist thereof, can be prepared into a synergist attracting core to be matched with the tea-wing stinkbug gathering pheromone attracting core for use, and can also be prepared into an attractant composition attracting core for use by mixing a tea-wing stinkbug gathering pheromone solution and the synergist solution; the multiple components are used together according to the proportion, and the autonomy is stronger.

(2) Compared with the lure containing only the tea bug aggregation pheromone without the synergist thereof, the lure containing the tea bug aggregation pheromone and the synergist thereof shows stronger trapping activity in a tea bug trapping test in the field.

(3) The synergist provided by the invention has low component price, can reduce the cost for manufacturing the lure and is suitable for popularization and promotion.

Detailed Description

The present invention is described in further detail below with reference to specific embodiments, which are given for the purpose of illustration only and are not intended to limit the scope of the invention.

Example 1

And performing a trapping effect test of the synergist and the gathering pheromone attracting core of the tea bug bugs in Tokyo village of corridor city of Hebei province from 7 months to 14 days in 2019.

The components of the synergist are as follows: the purities of linalool oxide, linalool, benzaldehyde and ethyl lactate are all more than 99%.

Benzaldehyde, linalool oxide and ethyl lactate are respectively dissolved in n-hexane at the concentrations of 5 mg/ml, 10 mg/ml and 20 mg/ml, 200 microliters of the solution is dripped on a septum of a rubber plug cleaned by the n-hexane, 200 microliters of dichloromethane is dripped on the septum of the rubber plug twice after the n-hexane is volatilized, and the synergist lure is prepared after the dichloromethane is volatilized. Packaging the obtained single component synergist core in aluminum foil, sealing, and storing at-20 deg.C.

Mixing the components according to the composition in the table 1, dissolving the components in n-hexane to prepare n-hexane solution of the multi-component synergist, wherein the concentration of the solution is 5 mg/ml, 10 mg/ml and 20 mg/ml respectively, dropwise adding 200 microliters of dichloromethane to a septum of a rubber plug cleaned by n-hexane respectively, dropwise adding 200 microliters of dichloromethane to the septum of the rubber plug twice after the n-hexane is volatilized, and preparing the synergist lure after the dichloromethane is volatilized. Packaging the obtained multiple-component synergist core in aluminum foil, sealing, and storing at-20 deg.C.

When a trapping test is carried out in the field, a synergist attracting core is fixed on an inverted leak type trap by a fine iron wire, the trap is hung on a pear branch with the height of 3-5 m, and meanwhile, an imported tea stink bug gathering pheromone attracting core (AgBio, DEAD-INN brand, made in America) or a self-prepared tea stink bug gathering pheromone attracting core ((3S,6S,7R,10S) -10,11-epoxy-1-bisabolen-3-ol and (3R,6S,7R,10S) -10,11-epoxy-1-bisabolen-3-ol weight ratio is 3.5:1) are used together. The reference substance of the series of comparative experiments is an imported tea-bug gathering pheromone lure (AgBio, DEAD-INN brand, made in America) or an auto-made tea-bug gathering pheromone lure. One tea bug gathering pheromone attracting core (a control group) or one tea bug gathering pheromone attracting core plus one synergist attracting core (a test group) is placed in each trap, and the weight ratio of the usage amount of the synergist to the usage amount of the tea bug gathering pheromone in the traps of the test group is 1:1, 1:2 or 1: 4. The trap spacing was about 50 meters, the traps were changed position every day, the number of caught tea bug bugs was recorded and cleared in time, three replicates per lure. The test results are shown in Table 2.

In the test data of table 2, groups a1-1 to E3-1 are the effect of using the synergist to lure the core in combination with the imported tea bug aggregation pheromone lure; groups A1-2 to E3-2 are the effects of using the synergist to lure the core in combination with the gathering pheromone lure core of the tea plant bug; the control group had the effect of attracting the core without the synergist. As can be seen from the table, after the lure containing the synergist of the tea bug aggregation pheromone of the present example was used, the trapping efficiency of the tea bug aggregation pheromone lure was significantly enhanced.

TABLE 1 composition of multicomponent synergists

Composition (I)	Molecular weight	Molecular formula	Standard sample concentration,%	Parts by weight
					Lactic acid ethyl ester	118.13	C5H10O3	100	0.5
Benzaldehyde	106.12	C7H6O	100	1.0
					Linalool	154.25	C10H18O	100	2.5
Linalool oxide	170.25	C10H18O2	100	6.5

TABLE 2 test data

Example 2

The tea wing bug attractant synergist comprises the following components in parts by weight: 5 parts of linalool oxide, 2 parts of linalool, 2 parts of benzaldehyde and 2 parts of ethyl lactate. And mixing the components according to the composition of the tea bug attractant synergist to prepare n-hexane solution of the multi-component synergist, wherein the concentration of the solution is 10 mg/ml, dropwise adding 200 microliters of the n-hexane solution onto a spacer of a rubber plug cleaned by n-hexane, dropwise adding 200 microliters of dichloromethane onto the spacer of the rubber plug twice after the n-hexane is volatilized, and preparing the synergist attractant core after the dichloromethane is volatilized. Packaging the obtained multiple-component synergist core in aluminum foil, sealing, and storing at-20 deg.C.

When the synergistic agent lure is subjected to a trapping test in the field according to the method in the example 1, the trapping efficiency of the aggregated pheromone lure is obviously enhanced after the lure containing the synergistic agent of the tea bug attractant is used.

Example 3

The tea wing bug attractant synergist comprises the following components in parts by weight: 8 parts of linalool oxide, 5 parts of linalool, 1.5 parts of benzaldehyde and 1 part of ethyl lactate. And mixing the components according to the composition of the tea bug attractant synergist to prepare n-hexane solution of the multi-component synergist, wherein the concentration of the solution is 10 mg/ml, dropwise adding 200 microliters of the n-hexane solution onto a spacer of a rubber plug cleaned by n-hexane, dropwise adding 200 microliters of dichloromethane onto the spacer of the rubber plug twice after the n-hexane is volatilized, and preparing the synergist attractant core after the dichloromethane is volatilized. Packaging the obtained multiple-component synergist core in aluminum foil, sealing, and storing at-20 deg.C.

When the synergistic agent lure is subjected to a trapping test in the field according to the method in the example 1, the trapping efficiency of the aggregated pheromone lure is obviously enhanced after the lure containing the synergistic agent of the tea bug attractant is used.

The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.