阅读说明：本技术 促进智力发育和肠道健康的母乳化婴幼儿配方奶粉及其制备方法和应用 (Maternal emulsified infant formula milk powder for promoting intelligence development and intestinal health and preparation method and application thereof ) 是由 袁庆彬 魏立华 荀一萍 王世杰 贾晓江 封肖颖 冯丽莉 张栋 薛玉玲 刘海荣 朱 于 2020-12-08 设计创作，主要内容包括：本发明属于婴幼儿食品领域,公开了一种促进智力发育和肠道健康的母乳化婴幼儿配方奶粉及其制备方法和应用,该配方奶粉是将牛乳/羊乳、脱盐乳清粉、复合植物油、复合母乳低聚糖、复合维生素和复合矿物质等混匀,溶解,均质,浓缩,喷雾干燥,再与乳铁蛋白、唾液酸、复合果粉和复合益生菌粉等混合均匀制得。本发明添加合理配比的多种复合成分,利用复合组分间协同增效,使本发明的配方奶粉活性成分更接近于母乳；本发明的配方奶粉用于代替母乳单独食用,或配合母乳食用,能促进智力发育和肠道健康。(The invention belongs to the field of infant food, and discloses a breast-emulsified infant formula milk powder for promoting intelligence development and intestinal health and a preparation method and application thereof. The active ingredients of the formula milk powder are closer to breast milk by adding a plurality of composite ingredients in reasonable proportion and utilizing the synergistic interaction among the composite ingredients; the formula milk powder of the invention is used for replacing breast milk to eat alone or matching with breast milk, and can promote intelligence development and intestinal health.)

1. The breast milk infant formula milk powder for promoting intelligence development and intestinal health is characterized in that raw materials for preparing active ingredients of the breast milk infant formula milk powder comprise the following components in parts by weight:

wet mixing: 35-55 parts of cow milk/goat milk, 20-35 parts of desalted whey powder, 0.5-8 parts of concentrated whey protein powder, 3-5 parts of lactose, 2-5 parts of anhydrous cream, 2-5 parts of composite vegetable oil, 3-5 parts of 1, 3-dioleate-2-palmitic acid triglyceride powder, 0.5-3 parts of composite breast milk oligosaccharide, 0.1-0.5 part of yeast beta-glucan, 0.1-1 part of phospholipid, 0.01-0.06 part of nucleotide, 0.1-0.5 part of composite vitamin and 0.1-0.3 part of composite mineral;

dry mixing: 0.5-3 parts of lactoferrin, 0.1-1 part of sialic acid, 0.1-0.5 part of DHA algae oil powder, 0.1-0.3 part of arachidonic acid powder, 0.1-1 part of compound fruit powder and 0.1-2 parts of compound probiotic powder.

2. The maternal emulsion infant formula for promoting intelligence development and intestinal health according to claim 1, wherein the composite vegetable oil is mixed by corn oil, grape seed oil, linseed oil and coconut oil in a weight ratio of 3-4:1-3:1-2: 2.

3. The maternal emulsion infant formula for promoting intelligence development and intestinal health according to claim 1 or 2, wherein the complex breast milk oligosaccharides are mixed from 2 ' -fucosyllactose, lacto-N-neotetraose, lacto-N-tetraose, 3 ' -sialyllactose and 6 ' -sialyllactose in a weight ratio of 4-9:2:2-4:1: 1-2.

4. The maternal emulsion infant formula for promoting intelligence development and intestinal health according to claim 1 or 2, wherein the vitamin complex comprises, by weight, 0.01-0.3 parts of vitamin a, 0.01-0.3 parts of vitamin D, 0.01-3 parts of vitamin E, 0.01-0.1 parts of vitamin K, and vitamin B₁0.1 to 1 portion of vitamin B₂0.1 to 1 portion of vitamin B₆0.01-0.3 part of vitamin B₁₂0.01-0.1 part, 0.08-24 parts of vitamin C, 0.01-0.3 part of pantothenic acid, 0.01-3 parts of nicotinic acid, 0.24-72 parts of choline and 0.01-0.1 part of biotin;

the compound mineral substance comprises 1-25 parts of calcium carbonate, 1-40 parts of potassium citrate, 1-20 parts of sodium chloride, 1-3 parts of magnesium sulfate, 0.01-0.1 part of ferrous sulfate, 0.01-0.1 part of potassium iodide, 0.01-0.1 part of zinc sulfate, 0.01-0.1 part of sodium selenite and 0.01-0.1 part of copper sulfate.

5. The maternal emulsion infant formula for promoting intelligence development and intestinal health according to claim 1 or 2, wherein the compound fruit powder is prepared by mixing grape powder, pineapple powder and kiwi powder in a weight ratio of 1-2:1: 1-2.

6. The maternal emulsion infant formula for promoting intelligence development and intestinal health according to claim 1 or 2, wherein the composite probiotic powder is active freeze-dried powder, and is prepared by mixing bifidobacterium animalis subsp lactis i797 powder, lactobacillus rhamnosus x253 powder, lactobacillus paracasei N1115 powder and lactobacillus plantarum N3117 powder in a weight ratio of 2-3:1:1-2: 1.

7. A process for preparing a maternal emulsion infant formula for promoting intelligence development and gut health according to any one of claims 1 to 6, wherein the maternal emulsion infant formula for promoting intelligence development and gut health is prepared by mixing wet mix, dissolving, homogenizing, concentrating, spray drying, and mixing with dry mix.

8. The method for preparing a maternal emulsion infant formula for promoting intelligence development and gut health according to claim 7, wherein prior to homogenization, it is preheated to 60-65 ℃; the pressure for homogenizing is 190-210 bar; sterilizing at 92-95 deg.C for 4-6s after homogenizing and before concentrating.

9. Method for preparing a maternal emulsion infant formula for promoting intelligence development and gut health according to claim 7 or 8, characterized in that said concentration conditions are:

the primary effect pressure is 300-320bar, and the primary effect temperature is 71-73 ℃;

the secondary pressure is 240-260bar, and the secondary temperature is 65-67 ℃;

the triple effect temperature is 61-63 ℃;

the condenser pressure is 167-.

10. Use of an intellectual development and gut health promoting maternal emulsion infant formula according to any one of claims 1 to 6, wherein said intellectual development and gut health promoting maternal emulsion infant formula is consumed alone or in combination with breast milk.

Technical Field

The invention belongs to the field of infant food, and relates to infant milk powder, in particular to maternal emulsified infant formula milk powder for promoting intelligence development and intestinal health, and a preparation method and application thereof.

Background

For various reasons, less than 35% of infants are fed with pure breast milk to 4 months of age worldwide, and infant formula has become an ideal substitute for infants without or with insufficient breast milk. The World Health Organization (WHO) advocates breast milk feeding of infants for at least 6 months after birth to ensure the growth and development of infants, improve immunity and prevent infectious diseases, and the development of infant formula milk powder always takes breast milk as the gold standard.

However, the formula milk powder still depends on cow milk, goat milk and the like, and due to species differences, besides increasing the food-borne allergy risk of infants, the animal-derived milk and the breast milk have great differences in amino acid composition, lipid structure, fatty acid content, content of other bioactive components and the like, and the differences are also key factors influencing the growth and development of the infants; because the infants are in a special physiological stage, the infants have incomplete digestive function, immature metabolic process and the like, which not only hinder the infants from absorbing and transporting non-breast milk nutrients, but also easily cause constipation, other gastrointestinal tract problems and even influence the intelligence development; at present, the nutrient components of the existing formula milk powder and breast milk still have certain differences, and particularly, a large amount of plant source components are added, so that the bioactive components are insufficient.

Therefore, the formula milk powder which is closer to breast milk and is beneficial to the intestinal health and intelligence development of infants is prepared by screening the added bioactive substances according to the nutritional composition of the breast milk and the growth and development requirements of infants and has important significance.

Disclosure of Invention

The invention aims to provide the maternal emulsified infant formula milk powder for promoting intelligence development and intestinal health, which supplements breast milk oligosaccharides and adds probiotics by simulating the protein composition and lipid ratio in Chinese breast milk so as to achieve the purposes of simulating bioactive substances contained in the breast milk to a greater extent, meeting the nutritional requirements of infant growth and development, ensuring the absorption and utilization of nutrient substances and promoting the intestinal health and intelligence development;

the invention also aims to provide a preparation method of the maternal emulsified infant formula milk powder for promoting intelligence development and intestinal health;

still another object of the present invention is to provide the use of the above-mentioned maternal emulsified infant formula for promoting intelligence development and intestinal health.

In order to achieve the purpose, the invention adopts the technical scheme that:

the mother emulsified infant formula milk powder for promoting intelligence development and intestinal health comprises the following raw materials of active ingredients in parts by weight:

wet mixing: 35-55 parts of cow milk/goat milk, 20-35 parts of desalted whey powder, 0.5-8 parts of concentrated whey protein powder, 3-5 parts of lactose, 2-5 parts of anhydrous cream, 2-5 parts of composite vegetable oil, 3-5 parts of 1, 3-dioleate-2-palmitic acid triglyceride powder, 0.5-3 parts of composite breast milk oligosaccharide, 0.1-0.5 part of yeast beta-glucan, 0.1-1 part of phospholipid, 0.01-0.06 part of nucleotide, 0.1-0.5 part of composite vitamin and 0.1-0.3 part of composite mineral;

dry mixing: 0.5-3 parts of lactoferrin, 0.1-1 part of sialic acid, 0.1-0.5 part of DHA algae oil powder, 0.1-0.3 part of arachidonic acid powder, 0.1-1 part of compound fruit powder and 0.1-2 parts of compound probiotic powder.

The cow/goat milk was accepted and pasteurized.

As a limitation, the composite vegetable oil is prepared by mixing corn oil, grape seed oil, linseed oil and coconut oil in a weight ratio of 3-4:1-3:1-2: 2.

As another limitation, the composite breast milk oligosaccharide is prepared by mixing 2 ' -fucose lactose, lactose-N-neotetraose, lactose-N-tetraose, 3 ' -sialyllactose and 6 ' -sialyllactose in a weight ratio of 4-9:2:2-4:1: 1-2.

As the third limitation, the compound vitamin comprises 0.01 to 0.3 portion of vitamin A, 0.01 to 0.3 portion of vitamin D, 0.01 to 3 portions of vitamin E, 0.01 to 0.1 portion of vitamin K and vitamin B in portion by weight₁0.1 to 1 portion of vitamin B₂0.1 to 1 portion of vitamin B₆0.01-0.3 part of vitamin B₁₂0.01-0.1 part, 0.08-24 parts of vitamin C, 0.01-0.3 part of pantothenic acid, 0.01-3 parts of nicotinic acid, 0.24-72 parts of choline and 0.01-0.1 part of biotin;

the compound mineral substance comprises 1-25 parts of calcium carbonate, 1-40 parts of potassium citrate, 1-20 parts of sodium chloride, 1-3 parts of magnesium sulfate, 0.01-0.1 part of ferrous sulfate, 0.01-0.1 part of potassium iodide, 0.01-0.1 part of zinc sulfate, 0.01-0.1 part of sodium selenite and 0.01-0.1 part of copper sulfate.

As a fourth limitation, the composite fruit powder is prepared by mixing grape powder, pineapple powder and kiwi powder in a weight ratio of 1-2:1: 1-2.

As a fifth limitation, the composite probiotic powder is active freeze-dried powder, and the number of viable bacteria is more than or equal to 10¹⁰The CFU/g is formed by the weight ratio of 2-3:1:1-2:1, namely mixing I797 bacterial powder of bifidobacterium animalis subspecies lactis, X253 bacterial powder of lactobacillus rhamnosus, N1115 bacterial powder of lactobacillus paracasei and N3117 bacterial powder of lactobacillus plantarum;

the Bifidobacterium animalis subsp lactis i797 used in the invention is preserved in the China general microbiological culture collection center (the national patent office appoints the patent microbiological collection center) of China microbiological culture collection management committee general microbiological collection center No. 3 of Beijing market Shangyang Beijing province Beijing Suzuo No.1, 8 and 20 days in 2019, and the preservation numbers are as follows: CGMCC No. 18403;

lactobacillus paracasei N1115(Lactobacillus paracasei N1115), which has been deposited in 17.3.2011 in Beijing, China general microbiological culture Collection center (national patent office assigned patent microbiological Collection center) of Sai Lu No. 3 located in the sunward area of Beijing, with the deposition numbers: CGMCC No.4691, which is disclosed in Chinese invention patent with the patent number of 201110357058.8 for the first time;

lactobacillus rhamnosus x253(Lactobacillus rhamnosus x253) has been deposited in the general microbiological culture collection center of the china microbiological culture collection administration (national patent office assigned patent microbiological collection center) of north west way No.1 hospital No. 3, north township, sunny region, 2019, with the deposition numbers: CGMCC No.18404, which has been disclosed in the Chinese invention patent with patent number 202010258772.0;

lactobacillus plantarum N3117(Lactobacillus plantarum N3117), which has been deposited at 5 days 12/2014 at the China general microbiological culture Collection center (national institute of patent and microorganism Collection) of Sai Lu No.1 Hospital 3, North Cheng, sunny, Beijing, with the deposit numbers: CGMCC No.10133, which is disclosed in the Chinese invention patent with the patent number of 201510936243.0 for the first time.

The invention also provides a preparation method of the maternal emulsified infant formula milk powder for promoting intelligence development and intestinal health, which comprises the steps of uniformly mixing wet mixed materials, dissolving, homogenizing, concentrating, spray drying, and uniformly mixing with dry mixed materials to obtain the maternal emulsified infant formula milk powder for promoting intelligence development and intestinal health.

As a limitation, preheating to 60-65 ℃ before homogenizing; the pressure for homogenizing is 190-210 bar; sterilizing at 92-95 deg.C for 4-6s after homogenizing and before concentrating.

As a second limitation, the conditions of the concentration are:

the primary effect pressure is 300-320bar, and the primary effect temperature is 71-73 ℃;

the secondary pressure is 240-260bar, and the secondary temperature is 65-67 ℃;

the triple effect temperature is 61-63 ℃;

the condenser pressure is 167-.

The invention also provides an application of the breast-emulsified infant formula milk powder for promoting intelligence development and intestinal health, and the breast-emulsified infant formula milk powder for promoting intelligence development and intestinal health is used for replacing breast milk to eat alone or used as supplementary food to be matched with the breast milk.

Due to the adoption of the technical scheme, compared with the prior art, the invention has the technical progress that:

(1) the invention adjusts the lipid structure in the milk powder by adding the compound vegetable oil with reasonable proportion, so that the fatty acid composition is closer to breast milk, the absorption of infants is facilitated, the oxidative decomposition of the fatty acid is utilized, the growth and development of the infant skeleton are promoted, and the brain nervous tissue development and the vision development are promoted;

(2) the compound breast milk oligosaccharide, the compound probiotic powder and the compound fruit powder which are reasonable in proportion are added at the same time, the three components are synergistic, the effect is better than that of any one compound which is used independently, nutrient substances and active ingredients in milk powder are closer to breast milk, brain development is promoted, in-vivo chronic inflammation is improved, intestinal health is maintained, the compound breast milk oligosaccharide and the compound probiotic powder are more suitable for a digestive system with incomplete infant development, and the physique of infants is enhanced;

(3) compared with plant source oligosaccharide in traditional milk powder, the breast milk oligosaccharide added in the invention enables the milk powder to be closer to breast milk components and can promote the proliferation of bifidobacterium in intestinal tracts of infants and maintain the stability of intestinal microecology, wherein 3 '-sialyllactose and 6' -sialyllactose are degraded by metabolism of probiotics to obtain sialic acid, thereby promoting the brain development of infants;

(4) the compound probiotic powder with reasonable proportion is added, so that the infant is helped to maintain intestinal steady state, the field planting of probiotics such as intestinal bifidobacteria and the like is promoted, the gastrointestinal tract problems of constipation and the like of the infant caused by incomplete development of the gastrointestinal tract and single dietary structure are relieved, the digestion and absorption of nutrient components are promoted, and meanwhile, the brain nerve development of the infant is promoted through the intestinal-brain axis connection;

(5) the compound fruit powder with reasonable proportion is added, the fruit powder has unique fruit flavor, can promote appetite of infants, has high nutritive value, is rich in anthocyanin, mineral substances, vitamins and the like, has the functions of resisting oxidation, promoting brain development and improving memory, and has rich nutrient components;

the breast milk powder for promoting intelligence development and intestinal health is used for replacing breast milk to eat alone or used as supplementary food to eat together with the breast milk; the preparation method is simple, the raw materials are wide in source and low in cost, and the preparation method is suitable for industrial production and is used for preparing the breast milk infant formula milk powder for promoting intelligence development and intestinal health.

Drawings

The invention will be described in further detail with reference to the accompanying drawings and specific embodiments;

FIG. 1 is a graph showing the results of probiotic proliferation experiments in example 8, in which the infant formula M1-M3 prepared in examples 1-3 according to the present invention is combined with conventional formula milk powder for promoting intelligence development and intestinal health;

FIG. 2 is a graph showing the results of culturing of embryonic mouse cerebral cortical neural stem cells OD450 in the control group and the experimental groups 1 to 4 in example 9;

FIG. 3 is a graph showing the results of weight gain of mice fed with the corresponding milk powder/milk in the conventional formula, breast milk and MY1-4 groups in example 10;

FIG. 4 is a graph showing the results of fecal flora abundance of mice fed the corresponding milk powder/milk in the conventional formula, breast milk and MY1-4 group in example 10;

FIG. 5 is a graph of the results of fecal IgA content for mice fed the corresponding milk powder/milk in the conventional formula, breast milk and MY1-4 groups in example 10;

FIG. 6 is a graph showing the results of serum IgA content in mice fed the corresponding milk powder/milk in the conventional formula, milk and MY1-4 groups in example 10.

Detailed Description

The present invention is further illustrated by the following specific examples, which are to be construed as merely illustrative, and not limitative of the remainder of the disclosure.

EXAMPLES 1-6A method for preparing a maternal emulsion infant formula for promoting intelligence development and intestinal health

Examples 1-6 a breast-emulsified infant formula for promoting intelligence development and intestinal health was prepared, respectively, comprising the following steps in sequence:

preparing composite components

Respectively preparing the composite components in the embodiments 1-6 in advance according to a composite component batching table shown in a table 1, and uniformly mixing the composite component batching in corresponding proportion to obtain corresponding composite components;

table 1 examples 1-6 compounding ingredient tables

In table 1, the proportional relationship 1 is the proportional relationship between corn oil, grape seed oil, linseed oil and coconut oil in the composite vegetable oil;

the proportional relation 2 is the proportional relation between 2 ' -fucose lactose, lactose-N-neotetraose, lactose-N-tetraose, 3 ' -sialyllactose and 6 ' -sialyllactose in the composite breast milk oligosaccharide;

the proportion relation 3 is vitamin A, vitamin D, vitamin E, vitamin K and vitamin B in the compound vitamin₁Vitamin B₂Vitamin B₆Vitamin B₁₂Vitamin C, pantothenic acid, nicotinic acid, choline and biotin;

the proportional relation 4 is the proportional relation among calcium carbonate, potassium citrate, sodium chloride, magnesium sulfate, ferrous sulfate, potassium iodide, zinc sulfate, sodium selenite and copper sulfate in the composite mineral substance;

the proportional relation 5 is the proportional relation among the grape powder, the pineapple powder and the kiwi fruit powder in the composite fruit powder;

the proportional relation 6 is the proportional relation among bifidobacterium animalis subspecies lactis i797 bacterial powder, lactobacillus rhamnosus x253 bacterial powder, lactobacillus paracasei N1115 bacterial powder and lactobacillus plantarum N3117 bacterial powder in the composite probiotic powder;

(II) preparing formula milk powder

The milk or goat milk, the desalted whey powder, the concentrated whey protein powder, the lactose, the anhydrous cream, the composite vegetable oil, the 1, 3-dioleate-2-palmitic acid triglyceride powder, the composite breast milk oligosaccharide, the yeast beta-glucan, the phospholipid, the nucleotide, the composite vitamin and the composite mineral are taken according to the formula raw material table of the examples 1-6 in the table 2, mixed uniformly, dissolved, homogenized, sterilized, concentrated, spray-dried and then mixed uniformly with the lactoferrin, the DHA algal oil powder, the arachidonic acid powder, the sialic acid, the composite fruit powder and the composite probiotic powder, and the specific process parameters are shown in the table 3, so that the breast-emulsified infant formula milk powder (correspondingly marked as M1-M6) for promoting intelligence development and intestinal health of the examples 1-6 is prepared.

Wherein cow milk or goat milk is standardized, subjected to impurity separation and pasteurized, and the obtained mother emulsified infant formula milk powder for promoting intelligence development and intestinal health is used as infant food; can be used for replacing breast milk to eat alone or as supplementary food to eat together with breast milk.

Table 2 examples 1-6 formula milk powder raw material table

Table 3 table of process parameters for examples 1-6

Example 7 fatty acid content of maternal emulsified infant formula for promoting mental development and gut health

Contents of palmitic acid, oleic acid, linoleic acid, α -linolenic acid, arachidonic acid, and DHA in nine groups of the breast milk-emulsified infant formula (labeled as MX1-MX 6), breast milk (labeled as MX 7), traditional milk formula (labeled as MX 8), and traditional goat milk powder (labeled as MX 9) prepared in examples 1-6 were measured using the method of GB 5009.168-2016 "determination of fatty acids in food", and the specific results are as follows:

table 4 fatty acid test results table

The results show that compared with the traditional cow/goat milk powder, the content and the proportion of the fatty acid in the breast milk-emulsified infant formula milk powder for promoting intelligence development and intestinal health are closer to breast milk, and the milk powder can be absorbed and utilized by infants more easily, so that the effects of supplementing fatty acid, promoting bone growth and development and being beneficial to brain nervous tissue and vision development are achieved.

Example 8 maternal emulsion infant formula to promote mental development and gut health to aid probiotic proliferation

28 newborn C57BL/6 mice, half male and half female, were selected and randomly divided into 4 groups:

a control group is filled with 0.2mL of traditional formula milk powder (the milk powder is not added with breast milk oligosaccharide, is added with galacto-oligosaccharide and fructo-oligosaccharide; the preparation method is according to the eating instruction);

dried group 1-3, intragastrically administered 0.2mL each day of the breast-emulsified infant formula milk powder M1-M3 (1 g milk powder dissolved in 10mL purified water, 50 deg.C warm water) for 3 weeks, prepared in examples 1-3, and used for promoting intelligence development and intestinal health, and the content of Bifidobacterium in feces of mice was measured at 0, 3, 5, 7, 9, 11, 13, 15, 21, and 28 days, and the results are shown in FIG. 1;

the results show that the content of bifidobacteria in the feces of the mice in the intervention group 1-3 is obviously higher than that of the control group, namely compared with the control group, the breast-emulsified infant formula milk powder for promoting intelligence development and intestinal health can better promote the proliferation of bifidobacteria in intestinal tracts of the mice and is beneficial to the establishment of intestinal flora of infants; among them, the breast milk formula M2 for promoting intelligence development and intestinal health prepared in example 2 has the best effect of promoting proliferation.

Example 9 synergistic Effect of Complex Breast milk oligosaccharides, Complex fruit powder and Complex probiotic powder on brain neural cells

Separating cerebral cortex tissue of fetal rat under aseptic condition to obtain single cell suspension, centrifuging to remove supernatant, and adding DMEM/F12 complete culture medium containing 2% B27 for culture; the fetal rat cerebral cortical neural stem cells with good growth state are randomly divided into five groups and inoculated into five 96-well plates, and the conditions of complete culture media used by each group are as follows:

control group: no composite breast milk oligosaccharide, composite fruit powder or composite probiotic powder is added;

experimental group 1: meanwhile, the compound breast milk oligosaccharide, the compound fruit powder and the compound probiotic powder which have the same proportion relation with the embodiment 2 are added;

experimental group 2: adding the same amount of composite breast milk oligosaccharide as the experimental group 1;

experimental group 3: adding the compound fruit powder with the same dosage as the experimental group 1;

experimental group 4: adding the composite probiotic powder with the same dosage as the experimental group 1;

after culturing for 48h under the same condition, measuring the OD450 of the cells by using CCK-8 kits respectively to show the cell proliferation condition;

the results are shown in fig. 2, compared with experimental groups 2-4, the experimental group 1 fetal rat cerebral cortical neural stem cell OD450 value is significantly larger, which indicates that the culture medium promotes the proliferation of cortical neural stem cells; namely, the compound breast milk oligosaccharide, the compound fruit powder and the compound probiotic powder are added simultaneously, so that the proliferation of cerebral cortical neural stem cells of a fetal rat can be better promoted, and the compound breast milk oligosaccharide, the compound fruit powder and the compound probiotic powder can be synergistic and can promote the growth of cerebral cortical tissues.

Example 10 synergistic Effect of Complex Breast milk oligosaccharides, Complex fruit powder and Complex probiotic powder on brain neural cells

48 newborn C57BL/6 mice, half male and half female, were selected and randomly divided into 6 groups of 8 mice each, each group was fed with the following formula milk powder/breast milk:

feeding traditional formula milk powder to a control group;

feeding breast milk by a breast milk group;

feeding MY1 group with the maternal emulsified infant formula milk powder M2 containing compound breast milk oligosaccharides, compound fruit powder and compound probiotic powder and prepared in example 2 for promoting intelligence development and intestinal health;

feeding formula milk powder without compound fruit powder and compound probiotic powder by MY2 group, wherein other components and preparation method of the formula milk powder are the same as those of the formula milk powder in example 2;

MY3 group is fed with formula milk powder without compound breast milk oligosaccharide and compound probiotic powder, and other components and preparation method of the formula milk powder are the same as those of the example 2;

sixthly, feeding formula milk powder without compound breast milk oligosaccharide and compound fruit powder by MY4 group, wherein other components and preparation method of the formula milk powder are the same as those of the embodiment 2;

continuously feeding for 3 weeks, and recording body weight every 2 days; collecting the excrement and tail vein blood of the mouse once every 7 days;

randomly selecting 4 mice in each group, and taking intestinal tissues of the mice in each group at 21 days to carry out ileum tissue T-AOC, MDA and CAT detection;

feeding the rest 24 weaning animals with common feed for 2 months, and performing water maze test, brain hippocampal tissue morphology observation and staining analysis;

the experimental results are as follows:

1) in the experimental process, the relationship between the multiple of the initial weight of the mouse and the feeding days is obtained according to the weight record, as shown in figure 3, the result shows that compared with the traditional formula milk powder, the maternal emulsified infant formula milk powder M2 for promoting intelligence development and intestinal health has the advantages that the growth rate of the mouse is accelerated after being fed, is similar to that of a breast milk group, and is beneficial to the growth and development of the mouse.

2) The results of the ileal tissue T-AOC, MDA and CAT assays are shown in Table 5:

TABLE 5 ileal tissue T-AOC, MDA and CAT test results Table

The results show that the ileum tissues T-AOC and CAT of the MY1 group mice are obviously higher than those of a control group, and the antioxidant stress capacity is strong; the feeding of the maternal emulsified infant formula milk powder M2 for promoting intelligence development and intestinal health is superior to the feeding of the traditional formula milk powder; T-AOC and CAT of mice fed with MY2-4 group were also higher than those of the control group, but lower than those of MY1 group; the compound breast milk oligosaccharide, the compound fruit powder and the compound probiotic powder are synergistically enhanced, so that the oxidative stress of the intestinal tract can be improved, and the compound breast milk oligosaccharide, the compound fruit powder and the compound probiotic powder have a better promotion effect on the intestinal tract health of mice.

3) The fecal flora abundance of the mice in the third week is detected, the result is shown in fig. 4, and the result shows that the fecal flora of the breast milk group and the MY1 group has higher content of two types of probiotics, namely bifidobacterium and lactobacillus, compared with the other groups; while the non-probiotic content of the genera clostridium, enterobacter and enterococcus is lower; compared with other groups, the composition of the intestinal flora of the mice in the MY1 group is more similar to that of the breast milk group, and the result shows that the mice in the MY1 group and the breast milk group have the same dietary structure, namely the breast emulsion infant formula milk powder M2 for promoting intelligence development and intestinal health is similar to the components of the breast milk.

4) The contents of fecal IgA and serum IgA of mice are detected, and the results are shown in figures 5-6, and the results show that the fecal IgA and serum IgA levels of mice in MY1 group are higher than those of a control group and MY2-4 group, the content of IgA immunoglobulin is higher, and high-level immunoglobulin shows that the immunity of the organism is better, namely the three composite nutrients of composite oligosaccharide breast milk, composite fruit powder and composite probiotic powder added in the invention have synergistic gain effect and can enhance the immunity of the mice.

5) The water maze test was performed on mice with the results shown in table 6:

as can be seen from Table 6, the time and the average swimming speed for the MY1 group to feed mice to find the safety island are higher than those of the control group, the index indicates the physique level of the mice, and the result indicates that the three compound nutrients of the compound breast milk oligosaccharide, the compound fruit powder and the compound probiotic powder added in the invention have the synergistic effect and can enhance the physique of the mice.

6) The result of observation and staining analysis of mouse brain hippocampal tissue morphology shows that the distribution density of cerebral hippocampal pyramidal cells of MY1 group mice is higher than that of other groups, the brain and intelligence development of the MY1 group mice are more similar to those of breast milk group, and the formula milk powder provided by the invention can enable the growth and development of the mice to be closer to those of breast milk-fed mice.

It should be noted that the above-mentioned embodiments are only preferred embodiments of the present invention, and are not intended to limit the present invention in other forms, and any person skilled in the art may use the above-mentioned technical content as a teaching to make changes or modifications to the equivalent embodiments with equivalent changes, but all those simple changes, equivalent changes and modifications made to the above-mentioned embodiments without departing from the technical spirit of the present invention, and still all those embodiments are within the scope of the present invention as claimed in the claims.