阅读说明：本技术 可降解微球及其应用 (Degradable microspheres and application thereof ) 是由 王建斌 王宏伟 尹俊龙 田梦 于 2019-05-31 设计创作，主要内容包括：一种可降解微球,其包括通过交联剂交联的聚合物分子。聚合物分子和/或交联剂含有敏感化学键,敏感化学键能够经化学和/或光处理而断裂从而导致可降解微球的降解。一种从样品中分离目标蛋白的方法,该方法利用可降解微球的降解替代蛋白纯化中的洗脱步骤,从而能够选用亲和力更强的目标蛋白和亲和配体组合以提高蛋白纯化效率。该方法适合于多个蛋白样品的高通量制备,例如为电镜观察或质谱测量提供蛋白样品。(A degradable microsphere comprising polymer molecules crosslinked by a crosslinking agent. The polymer molecules and/or the cross-linking agent contain sensitive chemical bonds that can be broken by chemical and/or light treatment resulting in degradation of the degradable microspheres. A method for separating target protein from a sample utilizes degradation of degradable microspheres to replace an elution step in protein purification, so that a combination of the target protein with stronger affinity and an affinity ligand can be selected to improve the protein purification efficiency. The method is suitable for high-throughput preparation of a plurality of protein samples, for example, protein samples are provided for electron microscope observation or mass spectrometry.)