阅读说明：本技术 酶法工业化生产nad的方法 (Method for industrially producing NAD (nicotinamide adenine dinucleotide) by enzyme method ) 是由 张章 张琦 于 2019-06-27 设计创作，主要内容包括：酶法工业化生产NAD的方法,该方法以烟酰胺核糖和ATP为原料,在NAD合成酶存在的条件下进行生物催化反应制备NAD,其中,NAD合成酶包含来源于流感嗜血杆菌的烟酰胺单核苷酸腺苷转移酶结构域以及来源于人类、酿酒酵母、大肠杆菌和鼠伤寒沙门氏菌中的任意一种的烟酰胺核糖激酶结构域。该方法可实现以NR和ATP为原料一步催化生产NAD。(The method for industrially producing NAD by enzyme method takes nicotinamide riboside and ATP as raw materials, and prepares NAD by biocatalytic reaction in the presence of NAD synthetase, wherein the NAD synthetase comprises nicotinamide mononucleotide adenyl transferase structural domain derived from haemophilus influenzae and nicotinamide ribokinase structural domain derived from any one of human, saccharomyces cerevisiae, escherichia coli and salmonella typhimurium. The method can realize one-step catalytic production of NAD by using NR and ATP as raw materials.)