阅读说明：本技术 一种防治谷子白发病的杀菌剂组合物 (Bactericide composition for preventing and treating white disease of foxtail millet ) 是由 张霞 张瑞 李�瑞 于 2020-12-04 设计创作，主要内容包括：本发明涉及农药技术领域,具体涉及一种防治谷子白发病的杀菌剂组合物。一种防治谷子白发病的杀菌剂组合物,其有效成分由香芹酚和苯醚菌酯、苯并烯氟菌唑或双炔酰菌胺二元复配而成。本发明的有效成分进行复配后,2种有效成分协同作用,具有显著的增效作用,可以提高对谷子白发病的防治效果,基于此可以减少农药的施用剂量,减少农药残留和降低环境压力。本发明将香芹酚和苯醚菌酯、苯并烯氟菌唑或双炔酰菌胺进行二元复配,使用时可以降低产生抗药性的风险,从而有效解决单一施用上述任一有效成分存在的缺陷。(The invention relates to the technical field of pesticides, and particularly relates to a bactericide composition for preventing and treating the white disease of foxtail millet. The active ingredients of the bactericide composition for preventing and treating the white disease of the foxtail millet are formed by binary compounding of carvacrol, kresoxim-methyl, benzovindiflupyr or mandipropamid. After the effective components are compounded, 2 effective components have synergistic effect, so that the synergistic effect is remarkable, the prevention and treatment effect on the white disease of millet can be improved, the application dosage of pesticide can be reduced, the pesticide residue can be reduced, and the environmental pressure can be reduced. According to the invention, carvacrol and kresoxim-methyl, benzovindiflupyr or mandipropamid are subjected to binary compounding, so that the risk of drug resistance can be reduced when the compound is used, and the defect of single application of any effective component can be effectively overcome.)

1. The bactericide composition for preventing and treating the disease of the millet white is characterized in that the active ingredients of the bactericide composition are prepared by binary compounding of carvacrol, kresoxim-methyl, benzovindiflupyr or mandipropamid.

2. The bactericide composition for preventing and treating the onset of the foxtail millet according to claim 1, wherein the mass ratio of carvacrol to kresoxim-methyl is 1-20: 40-1.

3. The bactericide composition for preventing and treating the onset of foxtail millet according to claim 1, wherein the mass ratio of carvacrol to benzovindiflupyr is 1-60: 40-1.

4. The bactericide composition for preventing and treating the onset of the foxtail millet according to claim 1, wherein the mass ratio of carvacrol to mandipropamid is 1-20: 20-1.

Technical Field

The invention relates to the technical field of pesticides, and particularly relates to a bactericide composition for preventing and treating the white disease of foxtail millet.

Background

For farmers who plant foxtail millet, the disease of foxtail millet is a common disease, and the pathogenic bacteria of the disease is foxtail millet. Pathogenic bacteria of the millet whitefly disease are mixed in manure and soil as oospores or adhered to the surface of seeds for overwintering, wherein soil-borne bacteria are main overwintering bacteria sources and can survive in the soil for 2-3 years generally. The later stage tissue of the diseased plant is cracked, the oospore overwinter on the soil, the diseased residual body and the manure seed, and the infection cycle is completed. The onset of the white millet disease shows different symptoms at different stages of the millet growth, which seriously affects the survival of millet seedlings and the heading of the millet, and further affects the yield of the millet.

Carvacrol is a pure botanical fungicide which is extracted and processed from a yellow flower bud, has a strong antibacterial effect, and can effectively inhibit the growth of pathogenic bacteria spores and hyphae; CN201510084511.0 discloses a bactericidal composition containing penflufen and carvacrol and application thereof, in particular to a bactericidal composition which has good control effect on powdery mildew or rust disease of wheat, fruit trees, flowers and vegetables after the penflufen and the carvacrol are compounded. A large number of indoor biological activity tests of the inventor show that after carvacrol and kresoxim-methyl, benzovindiflupyr or mandipropamid are compounded according to a certain proportion, the biological activity of the compound for the disease of the millet white shows a synergistic effect, and no related report about the compounding of carvacrol and kresoxim-methyl, benzovindiflupyr or mandipropamid exists at present.

The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.

Disclosure of Invention

The invention aims to provide a bactericide composition for preventing and treating the white disease of foxtail millet, which solves the problem caused by single application of one chemical agent.

In order to achieve the purpose, the invention provides the following technical scheme:

the active ingredients of the bactericide composition for preventing and treating the white disease of the foxtail millet are formed by binary compounding of carvacrol, kresoxim-methyl, benzovindiflupyr or mandipropamid.

Preferably, the mass ratio of carvacrol to kresoxim-methyl is 1-20: 40-1.

Preferably, the mass ratio of carvacrol to benzovindiflupyr is 1-60: 40-1.

Preferably, the mass ratio of carvacrol to mandipropamid is 1-20: 20-1.

Compared with the prior art, the invention has the following beneficial effects:

(1) after the active ingredients are compounded, 2 active ingredients have a synergistic effect, so that the synergistic effect is remarkable, the prevention and treatment effect on the white disease of millet can be improved, the application dosage of pesticide can be reduced, the pesticide residue can be reduced, and the environmental pressure can be reduced.

(2) According to the invention, carvacrol and kresoxim-methyl, benzovindiflupyr or mandipropamid are subjected to binary compounding, so that the risk of drug resistance can be reduced when the compound is used, and the defect of single application of any effective component can be effectively overcome.

Detailed Description

The technical solutions of the present invention are described in detail below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Examples: indoor bioactivity test after carvacrol compounding

Reagent to be tested: 83% of carvacrol technical, 96% of kresoxim-methyl technical, 96% of benzovindiflupyr technical and 92% of mandipropamid technical (Xiongda Nantong crop protection Co., Ltd.);

test subjects: mycelia collected from field foxtail millet white hair germ (Sclerospora graminicola (Sacc.) Schroeter) were isolated and purified in a laboratory, and cultured with a medium for use;

the test method comprises the following steps: (refer to NY/T1156.2-2006 "laboratory bioassay Standard for agricultural chemicals Fungicide part 2: inhibition of pathogenic fungi hypha growth test plate method")

1. Preparing a medicament: dissolving the raw materials with methanol, diluting with 0.1% Tween-80 solution, and setting the series mass concentration;

2. medicament treatment: under aseptic conditions, 10mL of the sterilized PDA culture medium which is melted in advance is added into an aseptic conical flask, 1mL of liquid medicine is quantitatively absorbed from low concentration to high concentration in sequence, and the liquid medicine is respectively added into the conical flasks and fully shaken. Then, the mixture was poured into 4 dishes having a diameter of 10cm in equal amounts to prepare drug-containing plates, and blank controls containing methanol and 0.1% Tween-80 were added to the plates, and 3 treatments were repeated for each treatment.

3. Inoculating and culturing: cutting a bacterial cake with the diameter of 5mm from the edge of a bacterial colony by using a sterilization puncher with the diameter of 5mm under the aseptic condition; inoculating the fungus cake in the center of the medicated plate with the hypha facing upwards, covering with a dish, and culturing at 25 deg.C in an incubator.

4. Investigation and calculation: when the diameter of the blank control colony is more than 2/3 of the diameter of the culture dish, measuring the diameter (unit: mm) of the colony by a caliper, vertically measuring the diameter of each colony by a cross method once, taking the average value, calculating the hypha growth inhibition rate of each treatment, performing regression analysis according to the concentration logarithm value of each medicament and the corresponding hypha growth inhibition rate value, calculating the EC50 of the medicament, and calculating the cotoxicity coefficient (CTC value) of the mixture according to the Tanbei method.

The calculation method comprises the following steps:

colony growth diameter-colony diameter-cake diameter (in this example, cake diameter is 5 mm);

the hypha growth inhibition ratio (%) [ (blank control colony growth diameter-medicament-treated colony growth diameter) ÷ blank control colony growth diameter ] × 100;

measured virulence index (ATI) ═ (standard agent EC50 ÷ test agent EC50) × 100;

theoretical virulence index (TTI) ═ a agent virulence index × percentage of a in the mixture + B agent virulence index × percentage of B in the mixture;

co-toxicity coefficient (CTC) × 100 [ actually measured toxicity index (ATI) ÷ theoretical toxicity index of the mixture (TTI) ].

Criteria are divided according to joint action:

the co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; 80< co-toxicity coefficient (CTC) <120 showed additive effect.

The results are shown in tables 1-3.

TABLE 1 determination of the indoor biological Activity of the Compound Cochinospermum bigeminum of carvacrol and Phenylkresoxim-methyl

Name and proportion of the medicament	EC50(mg/L)	ATI	TTI	CTC
					Carvacrol	1.64	100.00	——	——
Benzene kresoxim-methyl	5.86	27.99	——	——
					Carvacrol 1: kresoxim-methyl 40	3.58	45.81	29.74	154.02
Carvacrol 1: benzene kresoxim-methyl30	2.66	61.65	30.31	203.42
					Carvacrol 1: kresoxim-methyl 20	1.13	145.13	31.42	461.98
Carvacrol 1: benzene kresoxim-methyl 15	2.08	78.85	32.49	242.70
					Carvacrol 1: kresoxim-methyl 10	3.57	45.94	34.53	133.03
Carvacrol 1: benzene kresoxim-methyl 5	1.41	116.31	39.99	290.86
					Carvacrol 1: kresoxim-methyl benzene 1	1.20	136.67	63.99	213.56
Carvacrol 3: kresoxim-methyl benzene 1	0.72	227.78	82.00	277.79
					Carvacrol 5: kresoxim-methyl benzene 1	0.55	298.18	88.00	338.85
Carvacrol 7: kresoxim-methyl benzene 1	0.69	237.68	91.00	261.19
					Carvacrol 9: kresoxim-methyl benzene 1	0.84	195.24	92.80	210.39
Carvacrol 10: kresoxim-methyl benzene 1	0.93	176.34	93.45	188.70
					Carvacrol 20: kresoxim-methyl benzene 1	1.08	151.85	96.57	157.24

As can be seen from Table 1, the mass ratio of carvacrol to kresoxim-methyl is 1-20: the cotoxicity coefficients within the range of 40-1 are all larger than 120, namely the biological activity of the carvacrol and the kresoxim-methyl after being compounded to the disease of the millet white appears as a synergistic effect.

TABLE 2 indoor bioactivity assay of carvacrol and benzovindiflupyr combination on foxtail millet fungus

As can be seen from Table 2, the mass ratio of carvacrol to benzovindiflupyr is 1-60: the cotoxicity coefficients within the range of 40-1 are all larger than 120, namely the biological activity of the carvacrol and the benzovindiflupyr after being compounded shows synergistic effect on the white disease of the millet.

TABLE 3 determination of the indoor biological Activity of Cochinchinensis Scutellaria Evonoides and Biacetylenicamide

As can be seen from Table 3, the mass ratio of carvacrol to mandipropamid is 1-20: the cotoxicity coefficients within the range of 20-1 are all larger than 120, namely the biological activity of the compound of the carvacrol and the mandipropamid to the disease of the millet white appears as a synergistic effect.

In conclusion, when the carvacrol, the kresoxim-methyl benzoate, the benzovindiflupyr or the mandipropamid are compounded in a certain proportion, the biological activity of the compound preparation on the disease of the millet white is shown as a synergistic effect, the control effect of the disease of the millet white can be improved, and the application dosage of the pesticide can be reduced.

The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.