阅读说明：本技术 一种外源诱导组合物、外源诱导剂及提高茶叶中类胡萝卜素的方法 (Exogenous induction composition, exogenous inducer and method for improving carotenoid in tea ) 是由 施江 林�智 彭群华 王佳童 吕海鹏 于 2020-08-07 设计创作，主要内容包括：本发明涉及茶叶领域,特别是涉及一种外源诱导组合物、外源诱导剂及提高茶叶中类胡萝卜素的方法。本发明提供了一种外源诱导组合物,其特征在于,所述外源诱导组合物的有效成分包括茉莉酸甲酯、顺-3-己烯酯、顺-3-己烯醛、顺-3-己烯醇和反-2-己烯醛其中的一种或几种。采用本发明提供的外源诱导组合物能够显著提高茶叶中的类胡萝卜素总量,其中茶叶中类胡萝卜素中叶黄素含量在处理后的茶叶中含量提高幅度高达53％。(The invention relates to the field of tea, in particular to an exogenous induction composition, an exogenous inducer and a method for improving carotenoid in tea. The invention provides an exogenous induction composition, which is characterized in that the effective components of the exogenous induction composition comprise one or more of methyl jasmonate, cis-3-hexenyl ester, cis-3-hexenal, cis-3-hexenol and trans-2-hexenal. The exogenous induction composition provided by the invention can obviously improve the total amount of the carotenoid in the tea, wherein the content of the lutein in the carotenoid in the tea is improved by 53 percent in the processed tea.)

1. An exogenous induction composition, which is characterized in that the effective components of the exogenous induction composition comprise one or more of methyl jasmonate, cis-3-hexenyl ester, cis-3-hexenal, cis-3-hexenol and trans-2-hexenal.

2. An exogenous induction agent comprising ethanol and the exogenous induction composition of claim 1.

3. A method for increasing the carotenoid content in tea is characterized by comprising the following steps:

mixing the exogenous inducer of claim 2 with water to obtain a diluted exogenous inducer;

and spraying the diluted exogenous inducer on the leaves of the tea tree.

4. The method according to claim 3, wherein the spraying time of the exogenous inducer is 10-24 h before tea leaves are picked.

5. The method according to claim 3, wherein the volume concentration of the effective component in the diluted exogenous inducer is 0.1-0.2%.

6. The method as claimed in any one of claims 3 to 5, wherein the spraying amount of the diluted exogenous inducer is 6 to 22L/mu.

7. The method according to any one of claims 3 to 5, wherein the method further comprises the following steps after the spraying on the tea leaves: processing the fresh leaves after picking; the processed product comprises one or more of black tea, green tea and oolong tea.

Technical Field

The invention relates to the field of tea, in particular to an exogenous induction composition, an exogenous inducer and a method for improving carotenoid in tea.

Background

Carotenoids (carotenoids) in plants are the main substance basis for the bright yellow, orange and red colors of many horticultural crops, and in addition act as antenna pigments in photosynthesis transferring captured light energy to chlorophyll and have the functions of photoprotection and scavenging free radicals, protecting plant tissues from strong light. Moreover, research shows that the carotenoid is a synthetic precursor of phytohormones such as abscisic acid, strigolactone, carrot lactone and the like, and plays an important role in the growth and development of plants. The carotenoid is a main source of vitamin A in vivo, has the effects of resisting oxidation, regulating immunity, resisting cancer, delaying aging and the like, cannot be synthesized by a human body, and must be taken in through the outside.

Tea is one of three major beverages in the world, and more than 20 hundred million people consume tea leaves, which become a worldwide beverage. China is the home country of tea and is also a big country for tea production, consumption and export in the world. In the consumption and production of tea, the color of tea plays a very important role. The color and luster of the tea affect the quality such as color, aroma, taste and the like of the tea soup, and are the most intuitive factors for judging the quality of the tea. The content of carotenoid (carotenoids) in tea leaves accounts for about 0.1% of dry matter, and the carotenoid is an important component forming the appearance and the color of the bottom of the tea leaves and is a precursor of important aroma substances of the tea leaves. In addition, the highest content of lutein in tea has significant eye protection efficacy. Because the carotenoid mainly containing lutein is greatly degraded by heating in the tea processing process, the content of the carotenoid in the finished tea can only be about 25-35% of that in fresh tea at most, so that the carotenoid content in the tea sold in the market at present is generally low. The carotenoids mostly belong to water-insoluble substances, and the carotenoids cannot be completely absorbed and utilized by a common tea making way, but nowadays, the carotenoids are accepted to be eaten all over the world in a way of being gradually eaten from traditional tea drinking to the sun-flower tea, and the way can better absorb and utilize the functional components in the tea. Therefore, the improvement of the carotenoid content in the tea is one of the key qualities of the tea.

Disclosure of Invention

In view of the above, the invention provides an exogenous inducing composition, an exogenous inducer and a method for increasing carotenoid in tea leaves. The exogenous induction composition provided by the invention can obviously improve the carotenoid in the tea, wherein the content of lutein is improved by at least 33%.

In order to solve the technical problems, the invention provides the following technical scheme:

the invention provides an exogenous induction composition, wherein the effective components of the exogenous induction composition comprise one or more of methyl jasmonate, cis-3-hexenyl ester, cis-3-hexenal, cis-3-hexenol and trans-2-hexenal.

The invention provides an exogenous inducer, which comprises ethanol and the exogenous inducing composition of the technical scheme.

The invention provides a method for improving carotenoid in tea, which comprises the following steps:

mixing an exogenous inducer with water to obtain a diluted exogenous inducer;

and spraying the diluted exogenous inducer on the leaves of the tea tree.

Preferably, the spraying time of the exogenous inducer is 10-24 hours before tea leaves are picked.

Preferably, the volume concentration of the effective components in the diluted exogenous inducer is 0.1-0.2%.

Preferably, the spraying on the tea leaves further comprises: processing the fresh leaves after picking; the processed product comprises one or more of black tea, green tea and oolong tea.

Preferably, the spraying amount of the diluted exogenous inducer is 6-22L/mu.

The invention provides an exogenous induction composition, wherein the effective components of the exogenous induction composition comprise one or more of methyl jasmonate, cis-3-hexenyl ester, cis-3-hexenal, cis-3-hexenol and trans-2-hexenal. The exogenous induction composition provided by the invention adopts specific volatile matters containing carbonyl groups, can participate in the process of plant secondary metabolism, regulates and controls the synthesis of secondary metabolites of tea trees, can obviously improve the content of carotenoid in tea leaves, wherein the content of lutein is improved by 75%, and finally achieves the technical effect of improving the quality of the tea leaves.

In addition, the invention provides a use method of the exogenous inducer, the concentration of the effective components in the induction inhibitor used in the method is only 0.1-0.2%, the effect of improving the industrial quality can be achieved, and the method is simple and feasible, and is suitable for large-area tea leaf spraying.

Detailed Description

The invention provides an exogenous induction composition, wherein the effective components of the exogenous induction composition comprise one or more of methyl jasmonate, cis-3-hexenyl ester, cis-3-hexenal, cis-3-hexenol and trans-2-hexenal.

In the invention, the specific volatile matter containing hydroxyl can participate in the secondary metabolic process of plants, the synthesis of the secondary metabolites of tea trees is regulated and controlled, and the carotenoid content in tea leaves can be obviously improved.

In the present invention, when the active ingredient of the exogenous induction composition is a mixture of methyl jasmonate and cis-3-hexenyl ester, the mass ratio of methyl jasmonate to cis-3-hexenyl ester is preferably 1 to 7: 1-3, more preferably 1: 1.

in the invention, when the effective component of the exogenous induction composition is a mixture of cis-3-hexenol and cis-3-hexenal, the mass ratio of the cis-3-hexenol to the cis-3-hexenal is preferably 1-7: 1-3, more preferably 1: 1.

in the invention, when the effective component of the external assistance induction composition is a mixture of trans-2-hexenal and methyl jasmonate, the mass ratio of the trans-2-hexenal to the methyl jasmonate is preferably 1-7: 1-3, more preferably 1: 1.

in the invention, when the active ingredient of the exogenous induction composition is a mixture of methyl jasmonate, cis-3-hexenyl ester and cis-3-hexenol, the mass ratio of the methyl jasmonate to the cis-3-hexenyl ester to the cis-3-hexenol is preferably 1-2: 1-2: 1-2, more preferably 1.5: 1-1.1: 1 to 1.2.

In the invention, when the active ingredient of the exogenous induction composition is a mixture of methyl jasmonate, cis-3-hexenyl ester, cis-3-hexenal and cis-3-hexenol, the mass ratio of the methyl jasmonate to the cis-3-hexenyl ester to the cis-3-hexenal to the cis-3-hexenol is preferably 1-5: 1: 1: 1, more preferably 3: 1: 1: 1.

in the invention, when the active ingredient of the exogenous induction composition is a mixture of methyl jasmonate, cis-3-hexenyl ester, cis-3-hexenal, cis-3-hexenol and trans-2-hexenal, the mass ratio of the methyl jasmonate, the cis-3-hexenyl ester, the cis-3-hexenal, the cis-3-hexenol and the trans-2-hexenal is preferably 1-6: 1: 1: 1: 1, more preferably 4: 1: 1: 1: 1.

the invention provides an exogenous inducer, which comprises ethanol and the exogenous inducing composition of the technical scheme. The invention adopts ethanol as solvent, can fully dissolve effective components, and can form uniform oily liquid drops in aqueous solution, thereby ensuring the spraying effect. In the invention, the inducing composition is firstly dissolved in the ethanol, so that the condition that the substance is insoluble in water is avoided, and the inducing composition can be uniformly distributed and dissolved in the water in the subsequent use, thereby being beneficial to spraying.

The invention provides a method for improving carotenoid in tea, which comprises the following steps:

mixing the exogenous inducer in the technical scheme with water to obtain a diluted exogenous inducer;

and spraying the diluted exogenous inducer on the leaves of the tea tree.

The invention mixes the exogenous inducer with water to obtain the diluted exogenous inducer. In the present invention, the volume concentration of the active ingredient (exogenous induction composition) in the diluted exogenous inducer is preferably 0.1% to 0.2%, and more preferably 0.11% to 0.18%.

The diluted exogenous inducer is sprayed on the tea leaves. In the invention, the spraying time of the exogenous inducer is preferably 10-24 h before tea leaf picking, and more preferably 11-23 h. In the invention, the spraying amount of the diluted exogenous inducer is preferably 6-22L/mu, more preferably 6.25-22L/mu, and even more preferably 6.25-21L/mu.

In the present invention, after being sprayed on the tea leaves, the method preferably further comprises: processing the fresh leaves after picking; the product of the processing preferably comprises one or more of black tea, green tea and oolong tea.

The processing method of the present invention is not limited at all, and any processing method of black tea, green tea or oolong tea known in the art may be used.

To further illustrate the present invention, the exogenous inducing composition, exogenous inducing agent and method for increasing carotenoid in tea leaves provided by the present invention are described in detail below with reference to the examples, but they should not be construed as a precedent for the scope of protection of the present invention.