阅读说明：本技术 一种防止腹泻的益生菌配方 (Probiotic formula for preventing diarrhea ) 是由 苏友谊 于 2020-07-22 设计创作，主要内容包括：本发明公开了一种防止腹泻的益生菌配方,包括麦芽糊精、低聚半乳糖、银耳多糖、乳酸菌粉和葡萄糖酸锌,所述益生菌配方中各组分所占的百分比为：麦芽糊精10～20％、低聚半乳糖5～10％、银耳多糖5～10％、乳酸菌粉4～40％、葡萄糖酸锌0.1～1％和余量的蒸馏水,所述乳酸菌粉包括乳双歧杆菌1～10％、短双歧杆菌1～10％、罗伊氏乳杆菌1～10％和两歧双歧杆菌1～10％。该防止腹泻的益生菌配方,过合理的菌种的筛选,原料的搭配,可以在短时间内解决腹泻的问题,无副作用、止泻的效果理想、治疗时间短,成本低廉、所有原料都是食物来源,安全可靠,且配方中不包括乳基,也不包含乳粉、葡萄糖等过敏原,可以有效的避免乳糖不耐受和过敏的发生。(The invention discloses a probiotic formula for preventing diarrhea, which comprises maltodextrin, galacto-oligosaccharide, tremella polysaccharide, lactic acid bacteria powder and zinc gluconate, wherein the probiotic formula comprises the following components in percentage: 10-20% of maltodextrin, 5-10% of galacto-oligosaccharide, 5-10% of tremella polysaccharide, 4-40% of lactic acid bacteria powder, 0.1-1% of zinc gluconate and the balance of distilled water, wherein the lactic acid bacteria powder comprises 1-10% of bifidobacterium lactis, 1-10% of bifidobacterium breve, 1-10% of lactobacillus reuteri and 1-10% of bifidobacterium bifidum. The probiotic formula for preventing diarrhea can solve the problem of diarrhea in a short time by reasonably screening strains and matching raw materials, has no side effect, ideal antidiarrheal effect, short treatment time and low cost, all the raw materials are food sources, and is safe and reliable, and the formula does not contain milk base or allergens such as milk powder and glucose, so that lactose intolerance and allergy can be effectively avoided.)

1. A probiotic formula for preventing diarrhea comprises maltodextrin, galacto-oligosaccharide, tremella polysaccharide, lactic acid bacteria powder and zinc gluconate, and is characterized in that: the probiotic formula comprises the following components in percentage by weight: 10-20% of maltodextrin, 5-10% of galacto-oligosaccharide, 5-10% of tremella polysaccharide, 4-40% of lactic acid bacteria powder, 0.1-1% of zinc gluconate and the balance of distilled water.

2. The probiotic formulation for preventing diarrhea according to claim 1, wherein: the lactobacillus powder comprises 1-10% of bifidobacterium lactis, 1-10% of bifidobacterium breve, 1-10% of lactobacillus reuteri and 1-10% of bifidobacterium bifidum.

3. The probiotic formulation for preventing diarrhea according to claim 1, wherein: the preparation method of the tremella polysaccharide comprises the following steps:

s1, drying tremella in a dryer, crushing and filtering the dried tremella, and soaking the tremella in distilled water;

s2, extracting the tremella subjected to the S1 treatment under a high-temperature alkaline condition, and then filtering and collecting filtrate;

s3, carrying out fractional centrifugation and purification on the filtrate by adopting ethanol, and taking out supernatant after each centrifugation to obtain a centrifugal precipitation solution;

and S4, evaporating and vacuum-concentrating the solution after centrifugal precipitation, decoloring by a hydrogen peroxide method, dialyzing overnight, and freeze-drying to obtain the tremella polysaccharide.

4. The probiotic formulation for preventing diarrhea according to claim 3, wherein: in S3, when centrifugal purification is performed, the solution is first ultrasonically shaken and centrifuged at least for 20 minutes and at least three times each time.

5. The probiotic formulation for preventing diarrhea according to claim 1, wherein: the preparation steps of the galacto-oligosaccharide are as follows:

a, mixing a galactosidase solution with a lactose solution, uniformly stirring, and then adding the mixture into saccharomyces cerevisiae for fermentation to obtain a galactooligosaccharide solution;

b, adding the galactooligosaccharide solution into a nanofiltration membrane for filtering, and removing saccharomyces cerevisiae and protein contained in the solution to obtain galactooligosaccharide clear solution;

and C, finally, evaporating and concentrating the galactooligosaccharide clear solution to obtain galactooligosaccharide syrup.

6. The probiotic formulation for preventing diarrhea according to claim 5, wherein: in the step A, the mixing temperature of the galactosidase solution and the lactose solution is 35-40 ℃, the mixing time is 24-36 hours, and in the step B, the membrane aperture of the nanofiltration membrane is 200 Da-1000 Da.

7. The probiotic formulation for preventing diarrhea according to claim 1, wherein: the distilled water activity of the raw materials in the formula is 0.1-0.25 aw.

Technical Field

The invention belongs to the technical field of probiotics, and particularly relates to a probiotic formula for preventing diarrhea.

Background

The harm of diarrhea to human bodies is very large, especially to the problems of malnutrition, lactose intolerance, allergy, slow development and the like caused by long-term diarrhea of children, the treatment of antibiotics has shown many side effects, and at present, hospitals generally use the montmorillonite powder to solve the problem of diarrhea, but the montmorillonite powder has a certain effect on bacterial infection and is sometimes counterproductive to the problem of other dysbacteriosis. The probiotics has good effect on preventing and treating diarrhea through experiments and has no side effect.

However, glucose and milk powder are commonly added in the existing probiotic formula, the glucose can promote the growth of probiotics and harmful bacteria, the milk powder contains protein and lactose, and people with diarrhea caused by allergy and lactose intolerance cannot take the milk powder, so that the probiotics have certain disadvantages when eating, and meanwhile, the problem 1 of treating the diarrhea by only using the probiotics and the onset time are slow; 2. the effect of checking diarrhea is not ideal; 3. the treatment time is too long.

Disclosure of Invention

The present invention aims to provide a probiotic formula for preventing diarrhea, which solves the problems in the background art.

In order to achieve the purpose, the invention provides the following technical scheme: the probiotic formula for preventing diarrhea comprises maltodextrin, galacto-oligosaccharide, tremella polysaccharide, lactic acid bacteria powder and zinc gluconate, wherein the probiotic formula comprises the following components in percentage: 10-20% of maltodextrin, 5-10% of galacto-oligosaccharide, 5-10% of tremella polysaccharide, 4-40% of lactic acid bacteria powder, 0.1-1% of zinc gluconate and the balance of distilled water.

Preferably, the lactobacillus powder comprises 1-10% of bifidobacterium lactis, 1-10% of bifidobacterium breve, 1-10% of lactobacillus reuteri and 1-10% of bifidobacterium bifidum.

Preferably, the preparation method of the tremella polysaccharide comprises the following steps:

s1, drying tremella in a dryer, crushing and filtering the dried tremella, and soaking the tremella in distilled water;

s2, extracting the tremella subjected to the S1 treatment under a high-temperature alkaline condition, and then filtering and collecting filtrate;

s3, carrying out fractional centrifugation and purification on the filtrate by adopting ethanol, and taking out supernatant after each centrifugation to obtain a centrifugal precipitation solution;

and S4, evaporating and vacuum-concentrating the solution after centrifugal precipitation, decoloring by a hydrogen peroxide method, dialyzing overnight, and freeze-drying to obtain the tremella polysaccharide.

Preferably, in S3, when centrifugal purification is performed, the solution is first sonicated and centrifuged at least three times for at least 20 minutes each time.

Preferably, the preparation steps of the galactooligosaccharide are as follows:

a, mixing a galactosidase solution with a lactose solution, uniformly stirring, and then adding the mixture into saccharomyces cerevisiae for fermentation to obtain a galactooligosaccharide solution;

b, adding the galactooligosaccharide solution into a nanofiltration membrane for filtering, and removing saccharomyces cerevisiae and protein contained in the solution to obtain galactooligosaccharide clear solution;

and C, finally, evaporating and concentrating the galactooligosaccharide clear solution to obtain galactooligosaccharide syrup.

Preferably, in the step A, the mixing temperature of the galactosidase solution and the lactose solution is 35-40 ℃, the mixing time is 24-36 hours, and in the step B, the membrane aperture of the nanofiltration membrane is 200 Da-1000 Da.

Preferably, the distilled water activity of the raw materials in the formula is 0.1-0.25 aw.

The invention has the technical effects and advantages that: the probiotic formula for preventing diarrhea can well control the excessive fermentation of food by maltodextrin in the formula, simultaneously, the lactic acid bacteria powder can quickly adjust the flora balance in intestinal tracts, restore the microecology of the intestinal tracts to play the role of resisting bacteria and viruses, and can inhibit the propagation of harmful bacteria and promote the proliferation of probiotics by adding galacto-oligosaccharides, the tremella polysaccharide can quickly repair the mucosa of the intestinal tracts, and the zinc gluconate can supplement zinc elements lost in the diarrhea to promote the repair and healing of wound surfaces, can also stimulate the growth of B cells to increase the disease resistance of organisms, simultaneously, the zinc can promote the activity of disaccharidase in the intestinal tracts, in addition, the formula does not contain milk base, does not contain allergens such as milk powder, glucose and the like, can effectively avoid lactose intolerance and allergy, and finally, the probiotic formula for preventing diarrhea, can quickly solve the problem of diarrhea, has no side effect, ideal antidiarrheal effect, short treatment time and low cost, all the raw materials are food sources, and is safe and reliable.

Detailed Description

The technical solutions in the present invention will be described clearly and completely with reference to the contents of the present invention, and it is obvious that the described contents are only a part of the contents of the present invention, and not all of the contents. All other matters which can be obtained by a person skilled in the art without making creative efforts based on the contents of the present invention belong to the protection scope of the present invention.