阅读说明：本技术 一种利用复合酶制剂定向自大豆木质素间去除大豆蛋白的方法 (Method for directionally removing soybean protein from soybean lignin by using complex enzyme preparation ) 是由 安传世 于 2020-07-23 设计创作，主要内容包括：本发明公开了一种利用复合酶制剂定向自大豆木质素间去除大豆蛋白的方法,属于大豆蛋白提取技术领域。本发明利用复合酶制剂定向自大豆木质素间去除大豆蛋白的方法,利用复合酶制剂把木质素间夹杂的大豆蛋白分子定向从木质素上裂解下来,并把裂解下来维持大豆蛋白分子第3级、第四级结构稳定的键打开,让大豆蛋白分子溶解到水里,实现定向自大豆木质素间去除大豆蛋白。本发明生产工艺简单,生产效率高,经济效益好,具有较高的推广利用价值。(The invention discloses a method for directionally removing soybean protein from lignin of soybean by using a complex enzyme preparation, belonging to the technical field of soybean protein extraction. The method for directionally removing the soybean protein from the lignin of the soybean by using the complex enzyme preparation comprises the steps of directionally cracking the soybean protein molecules mixed between the lignins from the lignin by using the complex enzyme preparation, opening bonds for maintaining the stable 3 rd and fourth structures of the soybean protein molecules after cracking, and dissolving the soybean protein molecules into water to realize the directional removal of the soybean protein from the lignin of the soybean. The invention has simple production process, high production efficiency, good economic benefit and higher popularization and utilization value.)

1. A method for directionally removing soybean protein from soybean lignin by using a complex enzyme preparation is characterized by comprising the following steps:

(1) bean dreg dilution: diluting the wet bean dregs separated by the extraction separator to 2-5% of solid content, and keeping the temperature between 40-60 ℃;

(2) adjusting the pH value: adjusting the pH value of the bean dreg diluent to be between 8 and 10;

(3) adding enzyme for enzymolysis: adding complex enzyme preparation according to the proportion of 0.01-0.05%, controlling temperature for enzymolysis, and keeping for more than 30 minutes;

(4) centrifugal separation: separating the enzymolysis liquid by a horizontal spiral separator to obtain wet bean dregs with the protein content of less than 5 percent.

2. The method for directionally removing soybean protein from between soybean lignins by using the complex enzyme preparation as claimed in claim 1, wherein the optimal addition amount of the complex enzyme preparation in the step (3) is 0.02%.

3. The method for directionally removing soybean protein from between soybean lignins by using the compound enzyme preparation as claimed in claim 1, wherein the compound enzyme preparation is one or more of cellulase, pectinase, bromelain, alkaline protease and moderate temperature amylase.

4. The method for directionally removing soybean protein from between soybean lignins by using the complex enzyme preparation as claimed in claim 1, wherein the complex enzyme preparation comprises the following components: 10-20% of cellulase, 5-10% of pectinase, 20-40% of bromelain, 20-40% of alkaline protease and 10-30% of medium temperature amylase, wherein the sum of the components is 100%.

5. The method for directionally removing soybean protein from between soybean lignins by using the complex enzyme preparation as claimed in claim 1, wherein the complex enzyme preparation comprises the following components: 12.5% of cellulase, 7.5% of pectinase, 30% of bromelain, 30% of alkaline protease and 20% of medium-temperature amylase.

Technical Field

The invention relates to a method for directionally removing soybean protein from lignin of soybeans by using a complex enzyme preparation, in particular to a method for separating soybean protein and soybean fibers by using the complex enzyme preparation, belonging to the technical field of protein separation and extraction.

Background

In the process of extracting the soybean protein, a large amount of wet bean dregs are generated, about 2.5 tons of wet bean dregs can be generated per ton of soybean protein isolate, the solid content of the wet bean dregs is between 15 and 20 percent, the wet bean dregs contain about 13 to 20 percent (dry basis) of protein, and the protein existing in the wet bean dregs reduces the extraction rate of the soybean protein, reduces the quality guarantee period of the bean dregs and increases the difficulty of preparing soybean fiber by subsequent drying processing, thereby being a technical problem in the technical field of soybean processing.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention provides a method for directionally removing soybean protein from lignin of soybeans by using a complex enzyme preparation, which is characterized in that soybean protein molecules mixed in the lignin are directionally cracked from the lignin by using the complex enzyme preparation, bonds for maintaining the stable 3 rd and fourth structures of the soybean protein molecules after cracking are opened, the soybean protein molecules are dissolved in water, and the purpose of directionally removing the soybean protein from the lignin of the soybeans is achieved.

The invention realizes the technical effects through the following technical scheme:

a method for directionally removing soybean protein from soybean lignin by using a complex enzyme preparation specifically comprises the following steps:

(1) bean dreg dilution: diluting the wet bean dregs separated by the extraction separator to 2-5% of solid content, and keeping the temperature between 40-60 ℃;

(2) adjusting the pH value: adjusting the pH value of the bean dreg diluent to be between 8 and 10;

(3) adding enzyme for enzymolysis: adding complex enzyme preparation according to the proportion of 0.01-0.05%, controlling temperature for enzymolysis, and keeping for more than 30 minutes;

(4) centrifugal separation: separating the enzymolysis liquid by a horizontal spiral separator to obtain wet bean dregs with the protein content of less than 5 percent.

Preferably, the optimal addition amount of the complex enzyme preparation in the step (3) is 0.02%.

Further, the complex enzyme preparation is a mixed enzyme preparation of one or more of cellulase, pectinase, bromelain, alkaline protease and medium-temperature amylase.

Preferably, the complex enzyme preparation comprises the following components: 10-20% of cellulase, 5-10% of pectinase, 20-40% of bromelain, 20-40% of alkaline protease and 10-30% of medium temperature amylase, wherein the sum of the components is 100%.

Optimally, the complex enzyme preparation comprises the following components: 12.5% of cellulase, 7.5% of pectinase, 30% of bromelain, 30% of alkaline protease and 20% of medium-temperature amylase.

Compared with the prior art, the method for directionally removing the soybean protein from the soybean lignin by using the complex enzyme preparation can effectively separate the soybean protein from the soybean lignin in the bean dregs through the synergistic effect of the complex enzyme preparation, open the bond which is cracked to maintain the stable 3 rd and fourth structures of the soybean protein molecule, dissolve the soybean protein molecule into water and realize the directional removal of the soybean protein from the soybean lignin. The invention has simple production process, high production efficiency, good economic benefit and higher popularization and utilization value.

At present, the technology is converted and applied in a mountain pine company, the company produces 10 million tons of soybean protein isolate all the year round, wet bean dregs of about 25 million tons can be produced, wherein the average protein content of the wet bean dregs is 15 percent (dry basis), the protein content of the wet bean dregs is reduced to be below 5 percent after the technology is adopted, the protein yield is improved by more than 1.2 percent, 1200 tons of soybean protein isolate are produced, the sale amount of the soybean protein isolate is increased by more than 1800 million yuan RMB by the same raw material investment, and remarkable economic benefit is obtained.

Detailed Description