阅读说明：本技术 一种以生物酶相协同的打浆方法 (Pulping method with biological enzyme cooperation ) 是由 许在亮 吕亮 侯发财 许燕飞 徐岩 于 2020-07-24 设计创作，主要内容包括：本发明提供了一种以生物酶相协同的打浆方法。该技术方案首先对纸浆进行浸泡和搅拌,使其疏解分散至水中,而后加入特定活力的菠萝蛋白酶、木瓜蛋白酶、木聚糖酶、纤维素酶,调节温度至36℃,在超声震荡条件下搅拌酶解8h；而后,将酶解产物转入真空反应釜中,在负压条件下高温灭酶,同时促使纸浆中的蛋白成分变性,并接入绿色木霉和肠膜明串珠菌进行混合发酵,使纤维素得到一定程度的糖化,并使糖分得到分解；最后,利用水力碎浆机和磨浆机依次处理,得到打浆产物。本发明通过酶解结合活菌发酵的方式,在一定程度上降低了纤维的交联程度,并分解一部分糖类和蛋白,使后续的机械打浆更易执行,从而有效提高了打浆效率。(The invention provides a pulping method with biological enzyme synergy. According to the technical scheme, firstly, paper pulp is soaked and stirred to be defibered and dispersed into water, then bromelain, papain, xylanase and cellulase with specific activity are added, the temperature is adjusted to 36 ℃, and stirring enzymolysis is carried out for 8 hours under the ultrasonic oscillation condition; then, transferring the enzymolysis product into a vacuum reaction kettle, inactivating enzyme at high temperature under the condition of negative pressure, simultaneously promoting the denaturation of protein components in the paper pulp, and adding Trichoderma viride and Leuconostoc mesenteroides for mixed fermentation to saccharify cellulose to a certain degree and decompose sugar; and finally, sequentially treating the pulp by using a hydrapulper and a refiner to obtain a pulping product. According to the invention, through a mode of combining enzymolysis and viable bacteria fermentation, the crosslinking degree of the fibers is reduced to a certain extent, and a part of saccharides and proteins are decomposed, so that subsequent mechanical pulping is easier to execute, and the pulping efficiency is effectively improved.)

1. A pulping method with biological enzyme synergy is characterized by comprising the following steps:

1) mixing 100 parts by weight of paper pulp with 200 parts by weight of water, soaking for 2 hours, then stirring for 10 minutes in a stirrer at a rotating speed of 50rpm, adding bromelain to a final concentration of 220U/mL, papain to a final concentration of 180U/mL, xylanase to a final concentration of 460U/mL and cellulase 250U/mL, adjusting the temperature to 36 ℃, and continuing stirring for 8 hours under the ultrasonic oscillation condition;

2) adding the product obtained in the step 1) into a vacuum reaction kettle, keeping the temperature at 85 ℃ for 20min, naturally cooling to room temperature, inoculating trichoderma viride to the product until the final concentration is 10⁶CFU/mL, inoculated with Leuconostoc mesenteroides to a final concentration of 10⁵Per mL, fermenting for 24 hours at the temperature of 32 ℃;

3) and (3) adding the product obtained in the step 2) into a hydrapulper for treatment, and then pulping by using a refiner.

2. The bio-enzyme synergistic pulping method according to claim 1, wherein in step 1), after stirring for 8 hours, the thermostable amylase is added thereto to a final concentration of 280U/mL, and stirring is required for 2 hours.

3. The bio-enzyme-synergistic pulping method according to claim 1, wherein the soaking temperature in step 1) is 60 ℃, and the ultrasonic vibration treatment is continuously performed during the soaking.

4. The bio-enzyme-based pulping method according to claim 1, wherein the pressure of the vacuum autoclave in the step 2) is 0.3 atm during the holding at 85 ℃ for 20 min.

5. The bio-enzyme synergistic beating method according to claim 1, wherein in step 2), glucose is added to the culture at a final concentration of 0.5g/L at the time of fermentation up to 12 h.

6. The bio-enzyme synergistic pulping method according to claim 1, wherein in step 3), the refiner rotates at a speed of not less than 3000 rpm.

7. The bio-enzyme synergistic pulping method according to claim 1, wherein the pulp in step 1) is sisal pulp or manila pulp.

Technical Field

The invention relates to the technical field of pulping and papermaking, in particular to a pulping method with synergy of biological enzymes.

Background

Beating, also known as beating, is an operation in which pulp fibers suspended in water are mechanically treated to give them the characteristics required for production on a paper machine and to give the desired quality of the paper produced. Because the pulp fibers are stiff and elastic, if the pulp fibers are used for papermaking without any treatment, the pulp fibers are difficult to be uniformly distributed during forming on a wire, and the hardness of the papermaking paper is low. In addition, the pulp which is not pulped still contains unseparated fiber bundles which are stiff, too long, too thick and lack the necessary cutting and separation, and if the pulp is made of the fiber bundles, the obtained product is loose, porous, rough in surface and low in strength, and cannot meet the use requirements. The paper produced by the pulping paper pulp has compact and uniform tissue and higher strength.

In the beating process, the fibers are sheared, kneaded, carded and the like by a machine part, and meanwhile, the cell walls of the fibers are subjected to displacement, deformation, cracking and other phenomena to absorb water and swell to generate fine fibers, so that the paper pulp has softness and plasticity, the hydroxyl in the cellulose molecular chain is increased to be combined with hydrogen chains, and the binding force among the fibers is improved. According to the beating action, the method can be divided into sticky beating and free beating. The free beating is mainly cutting, and the concentration of paper pulp is lower. The beating machine of the method has sharper blades and dense pitch of the blades, and the fibers are easy to cut off during beating, so that the made paper has the characteristics of small density, bulkiness, opacity, small elasticity, easy adhesion of ink and the like. However, the paper produced by this method has low surface smoothness and is prone to fluffing. The sticky beating method uses the principle of breaking and grinding. The used paper pulp has higher concentration, the blades of the beater are duller, the blade pitch is sparse, and the phenomenon of different lengths is easy to generate when fibers are cut off, so that the manufactured paper has the characteristics of stronger physical property, compact paper, good surface smoothness of the paper, higher transparency, difficult scratching and the like. However, the paper has high elasticity and poor ink absorption effect. At present, the conventional pulping method only has mechanical action on fibers, but the fiber toughness is higher, so the conventional method generally has the problem of lower pulping efficiency.

Disclosure of Invention

The invention aims to overcome the technical defects in the prior art and provides a pulping method with biological enzyme cooperation so as to solve the technical problem that the conventional pulping method in the prior art is low in efficiency.

In order to achieve the technical purpose, the invention adopts the following technical scheme:

a pulping method with biological enzyme synergy comprises the following steps:

1) mixing 100 parts by weight of paper pulp with 200 parts by weight of water, soaking for 2 hours, then stirring for 10 minutes in a stirrer at a rotating speed of 50rpm, adding bromelain to a final concentration of 220U/mL, papain to a final concentration of 180U/mL, xylanase to a final concentration of 460U/mL and cellulase 250U/mL, adjusting the temperature to 36 ℃, and continuing stirring for 8 hours under the ultrasonic oscillation condition;

2) adding the product obtained in the step 1) into a vacuum reaction kettle, keeping the temperature at 85 ℃ for 20min, naturally cooling to room temperature, inoculating trichoderma viride to the product until the final concentration is 10⁶CFU/mL, inoculated with Leuconostoc mesenteroides to a final concentration of 10⁵Per mL, fermenting for 24 hours at the temperature of 32 ℃;

3) and (3) adding the product obtained in the step 2) into a hydrapulper for treatment, and then pulping by using a refiner.

Preferably, in the step 1), after stirring for 8 hours, the high temperature resistant amylase is added to the mixture until the final concentration is 280U/mL, and stirring is needed for 2 hours.

Preferably, in the step 1), the temperature of the soaking is 60 ℃, and the ultrasonic vibration treatment is continuously carried out during the soaking.

Preferably, in step 2), the pressure in the vacuum reaction vessel is 0.3 atm during the holding at 85 ℃ for 20 min.

Preferably, in step 2), glucose is added to the culture to a final concentration of 0.5g/L at the time of fermentation up to 12 h.

Preferably, in step 3), the rotation speed of the refiner is not lower than 3000 rpm.

Preferably, the pulp in step 1) is sisal pulp or manila pulp.

The invention provides a pulping method with biological enzyme synergy. According to the technical scheme, firstly, paper pulp is soaked and stirred to be defibered and dispersed into water, then bromelain, papain, xylanase and cellulase with specific activity are added, the temperature is adjusted to 36 ℃, and stirring enzymolysis is carried out for 8 hours under the ultrasonic oscillation condition; then, transferring the enzymolysis product into a vacuum reaction kettle, inactivating enzyme at high temperature under the condition of negative pressure, simultaneously promoting the denaturation of protein components in the paper pulp, and adding Trichoderma viride and Leuconostoc mesenteroides for mixed fermentation to saccharify cellulose to a certain degree and decompose sugar; and finally, sequentially treating the pulp by using a hydrapulper and a refiner to obtain a pulping product. According to the invention, through a mode of combining enzymolysis and viable bacteria fermentation, the crosslinking degree of the fibers is reduced to a certain extent, and a part of saccharides and proteins are decomposed, so that subsequent mechanical pulping is easier to execute, and the pulping efficiency is effectively improved.

Detailed Description

Hereinafter, specific embodiments of the present invention will be described in detail. Well-known structures or functions may not be described in detail in the following embodiments in order to avoid unnecessarily obscuring the details. Approximating language, as used herein in the following examples, may be applied to identify quantitative representations that could permissibly vary in number without resulting in a change in the basic function. Unless defined otherwise, technical and scientific terms used in the following examples have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.