阅读说明：本技术 一种皮肤过敏搔痒缓解液的配制及湿巾制备 (Preparation of skin allergy pruritus relieving liquid and preparation of wet tissue ) 是由 范建新 于 2020-04-12 设计创作，主要内容包括：本发明属于日化品及医药品领域,公开了一种皮肤过敏搔痒缓解液的配制及其湿巾制备方案,是以乳酸为主,以去离子水或纯水为溶剂,或可用盐酸、乳酸钠、醋酸、醋酸钠等调配,制成18℃时pH值介于1.50～2.11之间的酸性溶液,将所述酸性溶液涂抹在除眼睛外的皮肤搔痒部位,溶液中的氢离子使过敏原蛋白质的游离氨基、羟基饱和,从而使过敏原蛋白质发生一定程度的构象改变,而减弱与体内抗体特异性结合,致敏能力下降,从而减弱过敏反应,缓解搔痒感。也可用所述酸性溶液作湿巾液,在通用湿巾生产设备上,生产成特殊湿巾,或湿巾液与湿巾基材独立搭配成组装式湿巾。(The invention belongs to the field of daily chemicals and pharmaceuticals, and discloses a preparation method of a skin allergy pruritus relieving solution and a wet tissue preparation scheme thereof. The acid solution can also be used as wet tissue liquid to produce special wet tissue on general wet tissue production equipment, or the wet tissue liquid and a wet tissue base material are independently assembled into an assembled wet tissue.)

1. A skin allergy pruritus relieving solution is prepared by dissolving hydrochloric acid, lactic acid, sodium lactate, acetic acid, sodium acetate and the like in pure water by using a general chemical principle to prepare an acidic solution with a pH value of 1.50-2.11 at 18 ℃, and applying the acidic solution to skin pruritus parts except eyes, wherein free amino and hydroxyl of allergen protein are saturated by hydrogen ions in the solution, so that the allergen protein is subjected to a certain degree of conformational change to weaken specific binding with an in-vivo antibody, and the sensitization capability is reduced, thereby preventing and weakening anaphylactic reaction and relieving pruritus.

2. A wet tissue for relieving skin allergy and pruritus is prepared by dissolving hydrochloric acid, lactic acid, sodium lactate, acetic acid, sodium acetate and the like in deionized water or pure water to prepare an acidic solution with a pH value of 1.50-2.11 at 18 ℃ as a wet tissue solution, and producing finished wet tissues on a general wet tissue production device by taking a general wet tissue base material as a carrier, or independently combining the wet tissue solution and the wet tissue base material to form an assembled wet tissue, rubbing or applying the assembled wet tissue on an itching skin part, wherein free amino and hydroxyl of allergen protein are saturated by hydrogen ions in the wet tissue solution, the allergen protein is subjected to a certain degree of conformational change to weaken specific binding with in-vivo antibodies, the sensitization capability is reduced, and thus anaphylactic reaction is prevented and weakened, and itching feeling is relieved.

3. According to claim 1 or 2, characterized in that: the acid solution and the wet tissue liquid are prepared by lactic acid, and the amount concentration is 0.2-0.5 mol.L^-1In the meantime.

4. According to claim 1 or 2, characterized in that: the acid solution and the wet tissue liquid are prepared by lactic acid, and the optimal concentration is 0.4 mol.L^-1。

5. According to claim 1 or 2, characterized in that: the acid solution and the wet tissue liquid are prepared by hydrochloric acid and lactic acid, and the concentration of the lactic acid is 0.3 mol.L^-1The pH was adjusted with hydrochloric acid to 1.80 at 18 ℃.

6. The method according to claim 1 to 5, wherein: the acidic solution and the wet tissue liquid can be flavored by natural spice, extract or essence.

Technical Field

The invention belongs to the field of daily chemicals and pharmaceuticals, and discloses a preparation method of a skin allergy itching relieving and decomposing solution and a wet tissue preparation scheme.

Background

In summer and autumn, mosquitoes abuse, when heavenly stems and earthly leaves touch branches and bamboo leaves or walk from bushes, residues of insect bodies or plant tissue fragments are often the unknown itching-causing substances of people, namely human skin allergy, people scratch and scratch the itching, and the skin is easy to break and is easily infected.

After being bitten by mosquitoes, many human skins can be swollen, itchy, painful and the like, and the effects of toilet water, essential balm and relieving itching are not ideal. If the soap or the common soap is smeared by dipping water, a certain itching relieving effect is achieved, but in the field, the soap or the soap is inconvenient to carry and use, and the alkalinity destroys the natural ecological environment of the skin and hinders the health of the skin.

The floral water contains effective components of deet or deet, according to the related regulation of clinical use of medicine containing pesticide component, children under 6 years old, especially baby, because the immune system of skin is not completely established yet, the development of mucous membrane tissue of respiratory system of baby is not perfect, and long-term contact with product containing repellent is harmful to body.

Pollen allergy in spring is also a common phenomenon, pruritus is difficult to suffer, and oral antihistamine has certain effect.

Some antipruritic drugs such as cortisone ointment can relieve itching symptoms, but belong to adrenocortical hormone drugs and have certain strong side effects.

Boric acid has a certain effect, but when repeatedly used, accumulation can be caused, resulting in chronic poisoning, manifested as anorexia, asthenia, confusion, dermatitis, alopecia and menstrual disorder.

Disclosure of Invention

The invention aims to: aiming at the problems, the itching relieving liquid or the itching relieving wet tissue which is portable, has good effect of relieving skin allergy and pruritus and can be produced in a large scale is provided.

The invention relates to a preparation of a skin allergy pruritus relieving liquid, which is realized by adopting the following scheme: hydrochloric acid, lactic acid, sodium lactate, acetic acid, sodium acetate and the like are dissolved in deionized water or pure water by utilizing a general chemical principle to prepare an acidic solution with the pH value of 1.50-2.11 at 18 ℃, the acidic solution is smeared on the skin pruritus parts except eyes, and sufficient hydrogen ions in the solution are combined with free amino and hydroxyl of allergen to change the conformation of the allergen, so that the specific combination capacity with antibodies in a human body is weakened, the sensitization capacity of the allergen is reduced, the anaphylactic reaction is prevented and weakened, and the pruritus is relieved.

The invention relates to a preparation method of a skin allergy pruritus relieving wet tissue, which is realized by adopting the following scheme: hydrochloric acid, lactic acid, sodium lactate, acetic acid, sodium acetate and the like are dissolved in deionized water or pure water by utilizing a general chemical principle to prepare an acidic solution with the pH value of 1.50-2.11 at 18 ℃ as wet tissue liquid, a general wet tissue base material is used as a carrier on general wet tissue production equipment to produce finished wet tissue, or the wet tissue liquid and the wet tissue base material are independently assembled into an assembled wet tissue to be rubbed or applied on a skin pruritus part, and sufficient hydrogen ions in the solution are combined with free amino and hydroxyl of allergen to change the conformation of the allergen, so that the specific combination capacity of the allergen and the sensitization capacity of the allergen are reduced, and the anaphylactic reaction is prevented and reduced, and the itch is relieved.

Further, the acid solution and the wet tissue liquid are preferably prepared by using lactic acid and deionized water, and the concentration of the lactic acid is 0.2-0.5 mol.L^-1In between, it is preferable that the concentration of lactic acid is 0.4 mol. L^-1。

Further, the acid solution and the wet tissue liquid are preferably prepared by using lactic acid and deionized water, the pH value is adjusted to 1.8 by hydrochloric acid, and the content of the lactic acid is 0.3 mol.L^-1The hydrochloric acid-lactic acid antipruritic liquid is preferably selected.

Further, the acidic solution and the wet tissue liquid can be flavored by natural spice, extract or essence.

The invention relates to a preparation of a skin allergy pruritus relieving and itching relieving liquid and a preparation of a wet tissue thereof, and the principle is briefly described as follows:

the anaphylaxis is a specific immune response generated by the body after the human body is stimulated by the allergen again, and the response is quick but has individual difference. When it occurs on the skin, it is often red, swollen and itchy. The allergens are mostly foreign proteins, and if the conformation of the sensitization structure is destroyed, the immunogenicity of the common foreign proteins such as proteins contained in saliva, pollen and the like of mosquitoes can be destroyed, so that the aim of relieving itching is fulfilled.

The published data shows that female mosquitoes evolve and generate a piercing-sucking mouthpart formed by 6 mouthparts for breeding offspring, a tongue mouthpart with a hollow middle part is a saliva channel, saliva is injected into skin along the channel, enzymes and other proteins for preventing blood coagulation are contained in the saliva channel, skin allergic reaction is induced, the permeability of human skin blood vessels is stimulated to increase, and conditions are created for alternate piercing and blood sucking of other mouthparts. It is beneficial to mosquitoes, but is itching and difficult to decoct for people.

When extracting proteins or enzymes from biological tissues, a pH buffer must be used, otherwise the extracted proteins are denatured and inactivated, and thus, a natural buffer environment in which the proteins and enzymes function exists in the biological tissues. But the buffering capacity of any buffer is limited. A peracid or an overbased environment will destroy the protein conformation. The chemical principle of the buffer solution is reused and discussed in the abstract.

The buffer capacity (. beta.) is also called buffer index and is either a strong base db (mol) required to increase the pH of 1L of the buffer solution by 1dpH unit or a strong acid da (mol) required to decrease the pH of 1L of the buffer solution by 1dpH unit, and its mathematical formula is defined as shown in FIG. 1.

In HB-B^-In the buffer system, the total concentration of the buffer is c (mol. L)^-1) I.e. c = [ HB ]] + [B^-]When [ H ]⁺]、[OH^-]When the buffer capacity is small, the buffer capacity is approximated by the equations shown in fig. 2 and 3.

By taking the derivative of the formula in FIG. 2 and making the derivative equal to zero, an important data is obtained, i.e. when the buffer component [ HB ] is][B^-]Is 1: at 1 hour, there is a maximum buffer capacity beta_maxAnd occurs in Ka = [ H ]⁺]When is beta_max=0.575 c. Therefore, β_maxIs in direct proportion to the material volume concentration c in the system, namely when the concentration is higher, beta is_maxThe larger the value.

Calculation of HB-B according to the formula FIG. 3^-The buffer capacity of the buffer at different pH's varied as follows:

when pKa + -0.5 or 0.5 unit (10)^±0.5) I.e., [ HB ]]：[B^-]=3.16：1 or 1: at 3.16, β = 0.420c is calculated, and the buffer capacity is only β at this time_max73% (0.420 c/0.575 c).

When pKa + -0.75 or 0.75 unit deviation (10)^±0.75) I.e., [ HB ]]：[B^-]= 5.62: 1 or 1: at 5.62, calculated β = 0.295c, the buffer capacity is only β at this time_max51% of (0.295 c/0.575 c).

When pKa + -1 or 1 unit deviation (10)^±1) I.e., [ HB ]]：[B^-]= 10: 1 or 1: at 10, calculated as β =0.190c, the buffer capacity is only β at this time_max33% of (0.190 c/0.575 c).

When pKa + -1.5 or 1.5 units (10)^±1.5) I.e., [ HB ]]：[B^-]= 31.6: 1 or 1: at 31.6, calculated β = 0.0684c, the buffer capacity is only β at this time_maxAt the time of 12% (0.0684 c/0.575 c).

When pKa + -2 or 2 units of deviation (10)^±2) I.e., [ HB ]]：[B^-]= 100: 1 or 1: at 100, calculated β =0.0225c, at which time the buffer capacity is only β_max4% (0.0225 c/0.575 c) of (1).

When pKa + -3 or 3 units (10)^±3) I.e., [ HB ]]：[B^-]= 1000: 1 or 1: at 1000, calculate β = 0.00230c, where the buffer capacity is only β_max0.4% of (0.00230 c/0.575 c).

When pKa + -4 or 4 units of deviation (10)^±4) I.e., [ HB ]]：[B^-]= 10000: 1 or 1: 10000 times, calculated as beta = 0.000230c, and buffer capacity is only beta in this case_max0.04% (0.000230 c/0.575 c).

When pKa + -5 or 5 units of deviation (10)^±5) I.e., [ HB ]]：[B^-]= 100000: 1 or 1: on 100000, β = 0.0000230c is calculated, and in this case, the buffer capacity is only β_max0.004% of time (0.0000230 c/0.575 c), almost zero.

In summary of the above results, as shown in FIG. 4 (Table 1), it can be seen that the buffer capacity of the buffer changes sharply in a bell shape when the pH changes, as shown in FIG. 5,the solid line vertex coordinates in FIG. 5 are (pKa,. beta.)_max =0.575 c), significant decay at pKa ± 1 (β =0.190 c), β = 0.0000230c at pKa ± 5.

In summary, beta_maxIs in direct proportion to the material volume concentration c in the system, namely when the concentration is higher, beta is_maxThe larger the value; the buffer solution has a sharp change in its buffering capacity when the pH changes, and has a buffering capacity of zero when it deviates from the maximum buffering pH by 5 units.

The female mosquito saliva is a buffer system whose pKa values are inversely extrapolated from its environment of action. That is, as a result of natural selection, the environment is inevitably an appropriate environment for the female mosquito to act on the human skin tissue when the female mosquito sucks human blood, and the healthy human extracellular fluid has a pH value in the range of 7.35 to 7.45 and the pKa of the extracellular fluid at the tissue is 7.35, and therefore, the pKa of the female mosquito saliva is about 7.35.

In combination with the aforementioned graph of buffer capacity analysis, it can be seen that the buffer capacity of the buffer solution decreases to only 0.004% of the maximum buffer capacity when the pH exceeds pKa. + -. 5. Clearly, female mosquito saliva enters the skin of a human body through the salivary tract in very small amounts and in very low concentrations, and therefore, the buffer capacity of female mosquito saliva is very limited. When the pH value of the invaded part is rapidly changed to be more than 12.45 or less than 2.35, the space structure or conformation of the salivary protein can be rapidly destroyed, the space structure or conformation is changed and inactivated, the conformation causing human allergy is changed, and the sensitization force is obviously reduced. The itching caused by mosquito bites can be prevented by smearing alkaline soap or soda water on people.

The lactic acid is beneficial to maintaining normal ecology and health of skin, and the dominant lactic acid bacteria exist in the vagina of the unmarried female, so that the vagina is kept in a local slightly acidic environment, and the health of the vagina of the female is maintained. The gastric juice is acidic, the pH value of the normal gastric juice is 0.9-1.8, and the gastric juice can sterilize.

The hydrogen ions in the lactic acid solution saturate the free amino groups and hydroxyl groups of the allergen protein, the allergen protein undergoes a certain degree of conformational change, the specific binding with in vivo antibodies is weakened, and the sensitization capability of the allergen protein is reduced, so that anaphylactic reaction is prevented and weakened, and the itching feeling is relieved.

Therefore, the choice of lactic acid or hydrochloric acid or the mixed formulation of the lactic acid and the hydrochloric acid is a safe and effective choice for preparing the pruritus solution for relieving unknown reasons in the field, and lactate and acetate can be added to prepare a buffer solution, so that the pH value is kept between 1.5 and 2.11 at 18 ℃, but the material quantity is kept above a certain concentration range, and the skin is not damaged.

Several different lactic acid concentrations (mol. L)^-1) The pH values measured at 18 ℃ are shown in FIG. 6 (Table 2).

Drawings

Fig. 1 is a mathematical expression of the buffer capacity.

Fig. 2 is an approximate equation 1 for buffer capacity.

Fig. 3 is a buffer capacity approximation equation 2.

FIG. 4 is a table showing the change in buffer capacity of buffer solutions at different pH.

FIG. 5 is a bell plot of the buffer capacity of the buffer solutions at different pH.

FIG. 6 shows several different concentrations (mol. L) of lactic acid^-1) pH at 18 ℃.

FIG. 7 shows that 0.4 mol. L^-1The itching relieving effect of the lactic acid solution is compared with that of deionized water.

FIG. 8 is a graph showing the open type one-way sequential inspection (0.4 mol. L)^-1Lactic acid antipruritic liquid).

FIG. 9 shows that 0.35 mol. L^-1The itching relieving effect of the lactic acid solution is compared with that of deionized water.

FIG. 10 is a graph showing open type unidirectional sequential test (hydrochloric acid-lactic acid 0.3 mol. L)^-1Antipruritic liquid).

FIG. 11 is a graph showing the open type one-way sequential test (lactic acid 0.4 mol. L)^-1Itch relieving wet wipes).

In fig. 1: the solid line has vertex coordinates of (pKa,. beta.)_max=0.575 c), pKa ± 2 β =0.0225 c.

Detailed Description

The invention relates to a preparation of a skin allergy itching relieving liquid, which is illustrated by way of example.

Example 1 lactic acid (0.4 mol. L)^-1) Relieving itchingLiquid for treating urinary tract infection

Preparing a medicine: taking 18mL of analytically pure lactic acid into a 500mL volumetric flask, and using deionized water to perform constant volume to obtain lactic acid (0.4 mol. L)^-1) The itching relieving liquid is shown in Table 3.

TABLE 30.4 mol. L^-1The lactic acid itching relieving liquid has the distribution ratio and the pH value

Antipruritic liquid	Analytically pure lactic acid (m L)	Deionized water (m L)	pH value of 18 ℃ water bath
				Lactic acid (0.4 mol. L)-1）	18.0	482.0	2.00

Subpackaging: 50 spray bottles with the specification capacity of 20mL and the maximum liquid spray amount of 0.5mL per spray are taken, and the lactic acid anti-itching solution prepared in the table 3 is subpackaged for 20 for later use, wherein each bottle is 15mL, and the bottle bottoms are marked with A1-A20 in dark.

Preparation of drug for control group: 20 pet spray bottles with the same specification are taken and filled with 15mL of deionized water respectively, and the bottle bottoms are marked with B1-B20 in dark.

Experiment for preventing mosquito bite from itching

Experimental materials and tools

The artificially cultured Aedes albopictus is named as Aedes albopictus, square gauze cage of 600mm × 600mm × 600mm, movable sleeve, absorbent cotton, stopwatch and twenty plastic tweezers.

Aedes albopictus allergic healthy volunteers: male and female 10 times each, age 17-19 years old.

Double blind experimental procedure for antipruritic effect:

(1) randomly distributing 10 bottles A and 10 bottles B to 20 volunteers;

(2) the upper arm of the volunteer enters the artificial culture square gauze cage of the aedes albopictus from the movable sleeve opening, the aedes albopictus is observed to suck blood, the abdomen becomes red, and the arm is drawn out. Spraying the detection solution twice by using a spray bottle, spraying absorbent cotton twice, and rubbing the itching part for 0.2min by using the absorbent cotton;

(3) record of itching relief. The volunteers were observed to be relieved after 5min and 20min, and the sign "+ -" indicates that the effect is effective, and the sign indicates that the effect is not significant.

Results and conclusions

As a result: see FIG. 7 (Table 4), 0.4 mol. L^-1The itching relieving effect of the lactic acid solution is compared with that of deionized water.

And (4) conclusion: from the double blind test effect, lactic acid (0.4 mol. L)^-1) The itching relieving liquid has obvious itching relieving effect compared with deionized water blank control.

And (3) analysis: rubbing to make lactic acid fully contact with saliva and enzyme of mosquito, which are part of human allergen, and when the allergen is in strong acidity, the antigenic determinant is combined with hydrogen ion to change its space structure, so that it can not be combined with specific antibody in human body.

Second, field innominate factor allergy-induced skin pruritus and itching-relieving test

Experiment time: and (5) in summer.

The experimental method comprises the following steps: open unidirectional sequential test method.

Open type one-way sequential inspection diagram: the effective rate of itching relieving is 85%, the degree of confidence is 95%, the false positive is 5% (2 a = 0.05, the degree of confidence is 1- β = 0.95, and the effective rate θ = 0.85) are designed in advance, and a design inspection chart is prepared (see page 244 of 1979, 4 th date of dermatologic disease control research communication), YA =4.2+0.39x, YB = -3.4+0.39x, see fig. 8, the design inspection chart, YA =4.2+0.39x, YB = -3.4+0.39 x.

Test inclusion criteria: individuals with skin with occasional red, swollen and itchy parts (visual inspection) in the outdoor activity process are used as test subjects, the numbers are numbered according to the test sequence of volunteers, and 14 patients are selected for testing, wherein 9 patients are female, 5 patients are male, the age is 30-70 years, the pruritus is obvious, and other medicines are not smeared.

And (3) itching relieving experimental operation: spraying the detection solution twice in a spray bottle, spraying absorbent cotton twice, and rubbing the itching part with absorbent cotton for 0.2 min.

The effective standard is as follows: the effect is that the itching feeling is obviously reduced after 5min, otherwise the effect is not effective.

As a result: drawing test lines, sequentially filling the test lines into an open type one-way sequential inspection chart when the experimenter is completed according to requirements, wherein when an effective antipruritic person is obtained, the test lines are obliquely moved upwards to the right by one grid, an ineffective person is horizontally moved to the right by one grid, when the No. 9 is filled, the test lines are crossed with the effective line YA, and the result shows that the antipruritic liquid is effective, stopping the test, and showing in a chart 8.

And (4) conclusion: 0.4 mol. L is shown by an open type unidirectional sequential test method^-1The effect of lactic acid solution on relieving pruritus caused by wild innominate allergic pruritus can reach the expected target.

Example 2 lactic acid (0.35 mol. L)^-1) Preparation of itching relieving liquid and bamboo branch and leaf itching relieving experiment

Drug preparation

Preparation: 15.8mL of analytically pure lactic acid is taken in a 500mL volumetric flask, and the volume is determined by deionized water. To obtain lactic acid (0.35 mol. L)^-1) The itching relieving liquid is shown in Table 5.

TABLE 50.35 mol. L^-1The lactic acid itching relieving liquid has the distribution ratio and the pH value

Antipruritic liquid	Analytically pure lactic acid (m L)	DeionizationWater (m L)	pH value of 18 ℃ water bath
				Lactic acid (0.35 mol. L)-1）	15.8	484.2	2.03

Subpackaging: 50 bottles with a standard capacity of 20mL and a maximum liquid injection amount of 0.5mL per spray were used to prepare lactic acid (0.35 mol. L) according to Table 5^-1) The itching relieving liquid is divided into 20 bottles for standby, each bottle is 15mL, and the bottle bottoms are marked with A1-A20 in dark.

Preparation of drug for control group: 20 pet spray bottles of the same specification were added, each filled with 15mL of deionized water, the bottoms of which were labeled B1-B20 in dark.

Experimental materials and tools

A plurality of withered bamboo leaves at the tail end or the edge, absorbent cotton, a stopwatch and twenty plastic tweezers.

Experiment time: and (5) in summer.

Selecting allergic healthy volunteers: male and female 10 times each, age 17-19 years old.

Double blind experimental procedure for antipruritic effect:

(1) randomly distributing 10 bottles A and 10 bottles B to 20 volunteers;

(2) the upper arm of the volunteer is naked, and the brush is brushed for about 5 times by using the bamboo leaves with withered ends or edges, and the pain is stopped when the eyes are red and swollen or scratched;

(3) spraying the itching skin part twice with a detection liquid spray bottle, spraying absorbent cotton twice, and rubbing the itching skin part with the absorbent cotton for 0.2 min;

(4) record of itching relief. The volunteers were observed to be relieved after 5min and 20min, and the sign "+ -" indicates that the effect is effective, and the sign indicates that the effect is not significant.

Results and conclusions

As a result: see FIG. 9 (Table 6) 0.35 mol. L^-1The itching relieving effect of the lactic acid solution is compared with that of deionized water

And (4) conclusion: from the double blind test effect, lactic acid (0.35 mol. L)^-1) The itching relieving liquid has obvious itching relieving effect compared with deionized water blank control.

And (3) analysis: bamboo shoots withered at the ends or edges often carry remnants of worms, which become allergens for some people. Rubbing to make lactic acid fully contact with allergen, which is in strong acidity, free amino and hydroxyl of antigen determining group are combined with hydrogen ion to change its space structure, so that it can not be combined with antibody in human body, and the human body is not sensitive and can relieve itching.

EXAMPLE 3 preparation of hydrochloric acid-lactic acid antipruritic liquid and test for relieving itching

Preparation: the method is carried out according to the following table 7, using analytically pure lactic acid as main raw material, adjusting pH value with hydrochloric acid, and fixing volume with deionized water to obtain lactic acid (0.3 mol. L)^-1) The pH value of the water bath at 18 ℃ is 1.80.

TABLE 7 hydrochloric acid-lactic acid 0.3 mol.L^-1The ratio of components and pH value of the itching relieving liquid

Antipruritic liquid	Analytically pure lactic acid (m L)	Constant volume of deionized water (m L)	Adjusting pH value of hydrochloric acid in 18 ℃ water bath
				Lactic acid (0.3 mol. L)-1）	13.5	500	1.8

Itching relieving and itching relieving test for field innominate factor allergy

The experimental method comprises the following steps: open unidirectional sequential test method.

Open type one-way sequential inspection diagram: the effective rate of itching relieving is 85%, the degree of confidence is 95%, the false positive is 5% (2 α = 0.05, the degree of confidence is 1- β = 0.95, and the effective rate θ = 0.85), the inspection chart is designed in advance (see, specifically, communication on dermatologic disease control research 1979, No. 4, page 244), YA =4.2+0.39x, YB = -3.4+0.39x, as shown in fig. 10.

Test inclusion criteria: individuals with skin with occasional red, swollen and itchy parts (visual inspection) in the outdoor activity process are used as test subjects, the numbers are numbered according to the test sequence of volunteers, and 9 patients are selected for testing, wherein 6 patients are female, 3 patients are male, the age is 24-66 years, the pruritus is obvious, and other medicines are not smeared.

And (3) itching relieving experimental operation: spraying the detection solution twice in a spray bottle, spraying absorbent cotton twice, and rubbing the itching part with absorbent cotton for 0.2 min.

The effective standard is as follows: the itching feeling is obviously reduced after 5min, otherwise, the effect is not good.

As a result: test lines are drawn. When the experimenter is completed according to the requirement and is sequentially filled into the open type one-way sequential inspection chart, when an effective antipruritic is obtained, the test line is obliquely moved one grid upwards to the right, an ineffective one is horizontally moved one grid forwards and rightwards, when the No. 9 is filled, the test line is crossed with the effective line YA, and the result shows that the antipruritic liquid is effective, and the test is stopped, which is shown in a chart 10.

And (4) conclusion: hydrochloric acid-lactic acid (0.3 mol. L) by open type sequential test method^-1) The antipruritic effect on pruritus caused by wild unknown factor allergy reaches the expected target, and when the pH value is 1.80, hydrogen ions are more abundant and are combined with allergen, free amino and hydroxyl more fully, so that the antipruritic effect is better than that of single lactic acid.

Example 4 preparation of a skin allergy itching relieving Wet towel

The first step is as follows: wet wipes were prepared as in example 1.

The second step is that: and performing itching relieving and itching relieving test on wild unknown factor allergy.

Materials: compared with the scheme of the example 1, the method only uses the spunlace non-woven fabric to replace absorbent cotton, and the spunlace non-woven fabric is obtained by washing commercial wet tissues with deionized water in a reverse mode, and the rest is the same.

Experiment time: and (4) spring.

The experimental method comprises the following steps: open unidirectional sequential test method.

Open type one-way sequential inspection diagram: the effective rate of itching relieving is 85%, the degree of confidence is 95%, the false positive is 5% (2 α = 0.05, the degree of confidence is 1- β = 0.95, and the effective rate θ = 0.85), design inspection charts are prepared (see, specifically, communication of research and control of skin disease, 1979, No. 4, 244), YA =4.2+0.39x, YB = -3.4+0.39x, as shown in fig. 11.

Test inclusion criteria: individuals with skin with occasional red, swollen and itchy parts (visual inspection) in the outdoor activity process are used as test subjects, the numbers are numbered according to the test sequence of volunteers, and one part of each person is selected for testing, wherein 12 cases are total, 7 cases are female, 5 cases are male, the age is 25-71 years, the pruritus is obvious, and other medicines are not smeared.

And (3) itching relieving experimental operation: rubbing the itching part with spunlace nonwoven fabric for 0.2 min.

The effective standard is as follows: the itching feeling is obviously reduced after 5min, otherwise, the effect is not good.

As a result: test lines are drawn. When the experimenter is completed according to the requirement and is sequentially filled into the open type one-way sequential inspection chart, when an effective antipruritic is obtained, the test line is obliquely moved one grid upwards to the right, an ineffective one is horizontally moved one grid forwards and rightwards, when the No. 12 test line is filled, the test line is crossed with the effective line YA, and the result shows that the antipruritic liquid is effective, and the test is stopped, which is shown in a chart 11.

And (4) conclusion: lactic acid (0.4 mol. L) by open type unidirectional sequential test method^-1) The wet tissue achieves the expected antipruritic effect on pruritus caused by field unknown factor allergy and has better antipruritic effect.

And (3) analysis: the allergen binds to a specific antibody in vivo to cause an allergic reaction, and the binding is specific, so that when the allergen structure or the antibody structure is changed and cannot be bound, no allergic reaction occurs. Spring skin itchingMost pollen allergens are proteins in pollen, and free amino and hydroxyl contained in the proteins are combined with hydrogen ions of lactic acid to form 0.4 mol.L^-1The hydrogen lactate ions are enough to saturate free amino groups of pollen proteins, so that a certain degree of conformational change occurs, the sensitization capability is reduced, the anaphylactic reaction is prevented and weakened, and the itching feeling is relieved.

In conclusion, the preparation of the skin allergy pruritus relieving liquid and the preparation of the wet tissue thereof have clear principle and obvious actual effect, are suitable for large-scale production and are convenient for people to use; the skin allergy pruritus relieving and relieving liquid is used as a wet tissue liquid to be matched and assembled with a dry paper tissue, and the wet tissue is prepared by extrusion when in use, and is a good mode, and an auxiliary agent or essence and spice can be added for flavoring to improve the sense organ.

The above embodiments are merely examples of the present invention and are not intended to limit the scope of the present invention.