阅读说明：本技术 一种香菇菌株及其培育方法和应用 (Lentinus edodes strain and cultivation method and application thereof ) 是由 梁晓生 梁宏丽 柳凤玉 刘桂娟 于 2020-07-27 设计创作，主要内容包括：本发明涉及食用菌培育技术领域,具体公开一种香菇菌株及其培育方法和应用。所述香菇菌株的菌种保藏号为CGMCC No.19652,其培育方法为将所述香菇菌株接种到母种培养基上培养得到母种菌丝,将所述菌丝接种到原种培养基中扩大培养得到原种菌丝,再将所述原种菌丝接种到菌袋培养基中进行菌袋培养和出菇管理。本发明香菇菌株与传统的香菇品种相比,兼具产量高、优质菇比例高、染菌率低、生物转化率高以及单株生长的特点,适合全国大范围推广种植。(The invention relates to the technical field of edible fungus cultivation, and particularly discloses a mushroom strain and a cultivation method and application thereof. The strain preservation number of the lentinus edodes strain is CGMCC No.19652, and the cultivation method comprises the steps of inoculating the lentinus edodes strain to a mother strain culture medium to culture to obtain mother strain hypha, inoculating the hypha to a stock strain culture medium to perform enlarged culture to obtain stock strain hypha, and then inoculating the stock strain hypha to a fungus bag culture medium to perform fungus bag culture and fruiting management. Compared with the traditional mushroom variety, the mushroom strain has the characteristics of high yield, high ratio of high-quality mushrooms, low contamination rate, high biotransformation rate and single-plant growth, and is suitable for large-scale popularization and planting in China.)

1. A shiitake mushroom strain characterized by: the preservation number of the strain is CGMCC No. 19652.

2. Protoplasts produced by the shiitake strain of claim 1.

3. Spores produced by the mushroom strain of claim 1.

4. Mycelium produced by the shiitake mushroom strain of claim 1.

5. Fruiting body produced by a strain of lentinus edodes as claimed in claim 1.

6. A shiitake stick comprising the shiitake strain of claim 1.

7. Use of a shiitake strain according to claim 1 for the breeding of shiitake mushrooms.

8. Use of a shiitake strain according to claim 1 for the preparation of shiitake fruiting bodies, shiitake mycelia and/or shiitake spores.

9. A method of growing a strain of lentinus edodes according to claim 1, wherein: inoculating the preserved strain or fruiting body of the lentinus edodes strain to a mother culture medium to culture to obtain mother strain hypha, inoculating the hypha to a stock culture medium to perform enlarged culture to obtain stock strain hypha, and then inoculating the stock strain hypha to a fungus bag culture medium to perform fungus bag culture and fruiting management.

10. A cultivation method as claimed in claim 9, characterised in that: the culture temperature of the mother hyphae and the stock hyphae and the culture temperature of the fungus bags are both 20-30 ℃; and/or

The environmental humidity of the original strain hypha culture and the fungus bag culture is 60-70%; and/or

The mother culture medium comprises the following components in percentage by weight: potato at 220g/L, glucose at 15-25g/L, KH₂PO₄2-4g/L，MgSO₄·7H₂0.4-0.6g/L of O, 18-22g/L of agar and natural pH; and/or

The stock culture medium comprises the following components in parts by mass: 75-80 parts of wood chips, 15-25 parts of wheat bran, 0.5-1.5 parts of gypsum, 0.5-1.5 parts of brown sugar, 120 parts of water and 150 parts of water, and the pH is natural; and/or

The fungus bag culture medium comprises the following components in parts by mass: 78-80 parts of wood chips, 15-25 parts of wheat bran, 0.5-1.5 parts of gypsum, 120 parts of water and 150 parts of water, and the pH is natural.

Technical Field

The invention relates to the technical field of edible fungus cultivation, in particular to a mushroom strain and a cultivation method and application thereof.

Background

Lentinus edodes (Lentinuseedodes) is an edible fungus, contains rich dietary fiber, protein, polysaccharide, amino acid and other nutrients, and has the functions of improving immunity, delaying senility, preventing and resisting cancer, lowering blood pressure, reducing blood fat, reducing cholesterol, reducing blood sugar, etc.

At present, the varieties of the shiitake mushrooms are various all over the country, but the work in the aspects of breeding and oriented cultivation of excellent strains is less, and the backup varieties are deficient. 808 and 168 of main mushroom cultivars in China belong to medium-large hard high-quality mushroom varieties and are used as early as 80 years in the last century, but the 808 and 168 have long growth and development periods, and the degradation problems of yield reduction, poor stress resistance, aggravation of diseases and insect pests, increase of deformed mushrooms and the like already occur in the long-term passage process. The growth and development periods of 0912 and 868 in the early-maturing shiitake variety are short, the fruiting amount is large, but the quality of the shiitake is seriously influenced due to excessive and dense fruiting. Meanwhile, the four mushroom cultivars 808, 168, 0912 and 868 have the problem of high clustering rate, so that deformed mushrooms are easy to appear and the overall quality of the mushrooms is influenced.

Disclosure of Invention

The invention provides a shiitake strain and a cultivation method and application thereof, aiming at the problems of low yield, poor anti-bacterial capability and high clustering rate of the existing shiitake variety.

In order to achieve the purpose of the invention, the embodiment of the invention adopts the following technical scheme:

a Lentinus edodes strain with preservation number of CGMCC No.19652 is provided. The strain is obtained by selecting and breeding hybrid progeny of which the parents are shiitake mushroom 168 and shiitake mushroom 0912, belongs to shiitake mushrooms (Lentinula edodes), is named as Pingxiang No. 3, is preserved in the common microorganism center of China general microbiological culture Collection center in 04.06.2020, and the preservation address is located in the microbial research institute of China academy of sciences No. 3 of the Xilu No.1 Hopkins of the sunward area, Beijing.

Compared with the traditional mushroom variety, the mushroom strain provided by the invention has the characteristics of high yield, high ratio of high-quality mushrooms, low contamination rate, high biotransformation rate and single plant growth (low clustering rate), is a novel high-quality mushroom variety, and is suitable for large-scale popularization and planting in China.

The invention also provides protoplasts produced by the mushroom strains.

The invention also provides spores produced by the mushroom strains.

The invention also provides mycelium produced by the mushroom strain.

The invention also provides a fruiting body produced by the mushroom strain.

The invention also provides a shiitake mushroom stick containing the shiitake mushroom strain.

The invention also provides application of the mushroom strain in mushroom breeding.

The invention also provides application of the shiitake mushroom strain in preparation of shiitake mushroom fruiting bodies, shiitake mushroom mycelia and/or shiitake mushroom spores.

The invention also provides a cultivation method of the mushroom strain. The cultivation method at least comprises the following steps:

inoculating the preserved strain or fruiting body of the mushroom strain to a mother culture medium to culture to obtain mother strain hypha, inoculating the mother strain hypha to a stock culture medium to perform enlarged culture to obtain stock strain hypha, and inoculating the stock strain hypha to a fungus bag culture medium to perform fungus bag culture and fruiting management. Wherein, the fungus bag culture and fruiting management methods are conventional methods for culturing the existing mushrooms.

Preferably, the culture temperature of the mother spawn hyphae and the stock spawn hyphae and the culture temperature of the fungus bags are both 20-30 ℃.

Preferably, the environmental humidity of the culture of the stock hyphae and the culture of the fungus bags is 60-70%.

Preferably, the formula of the mother culture medium is as follows: potato at 220g/L, glucose at 15-25g/L, KH₂PO₄2-4g/L，MgSO₄·7H₂0.4-0.6g/L of O, 18-22g/L of agar and natural pH.

Preferably, the stock culture medium comprises the following components in parts by mass: 75-80 parts of wood chips, 15-25 parts of wheat bran, 0.5-1.5 parts of gypsum, 0.5-1.5 parts of brown sugar, 120 parts of water and 150 parts of natural pH.

Preferably, the fungus bag culture medium comprises the following components in parts by mass: 78-80 parts of wood chips, 15-25 parts of wheat bran, 0.5-1.5 parts of gypsum, 120 parts of water and 150 parts of water, and the pH is natural.

Drawings

FIG. 1 is a photograph of antagonistic lines of ZJ3 and 168 strains in the antagonistic test of example 2 of the present invention;

FIG. 2 is a photograph of antagonistic lines of ZJ3 and 0912 strains in the antagonism assay of example 2 of the present invention;

FIG. 3 is a diagram showing the growth state of first tide mushrooms of the strain ZJ3 in example 2 of the present invention;

FIG. 4 shows the first tide mushroom morphology of ZJ3 with parent strains 168 and 0912 in the new hybrid strain of example 2 of the invention as shown in FIG. 4.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.