阅读说明：本技术 一种细胞图像处理系统、方法、自动读片装置与存储介质 (Cell image processing system, cell image processing method, automatic film reading device and storage medium ) 是由 叶波 余珊 陈巧妮 邢圆 祁欢 叶燚 于 2018-07-13 设计创作，主要内容包括：本发明公开了一种细胞图像处理系统,包括：控制装置,配置为调整数字成像装置与涂片的相对位置；数字成像装置,包括透镜组和数字相机；其中,数字相机配置为相对于涂片的平面发生竖直方向的移动时,拍摄透镜组捕获的视野中细胞的不同对焦深度的图像；显示输出装置,配置为向显示界面输出至少一个视野的不同对焦深度的细胞图像。本发明还公开了一种细胞图像处理系统中的细胞图像处理方法、自动读片装置和存储介质。(The invention discloses a cell image processing system, comprising: a control device configured to adjust a relative position of the digital imaging device and the smear; a digital imaging device including a lens group and a digital camera; wherein the digital camera is configured to take images of different depths of focus of cells in the field of view captured by the lens group upon movement in a vertical direction relative to the plane of the smear; a display output device configured to output the cell images of the different in-focus depths of the at least one field of view to a display interface. The invention also discloses a cell image processing method, an automatic film reading device and a storage medium in the cell image processing system.)

A cell image processing system, the cell image processing system comprising:

a control device configured to adjust a relative position of the digital imaging device and the smear;

the digital imaging device comprises a lens group and a digital camera;

wherein the content of the first and second substances,

the digital camera is configured to take images of different depths of focus of cells in a field of view captured by the lens group upon vertical movement relative to the plane of the smear;

a display output device configured to output the cell images of the different in-focus depths of the at least one field of view to a display interface.

The cytological image processing system of claim 1, wherein the digital imaging device is adapted to take images of cells in different fields of view in the smear formed by the lens assembly upon movement in a parallel direction relative to the plane of the smear.

The cellular image processing system according to claim 1 or 2, wherein the cellular image processing system includes a normal mode and a depth of field mode, wherein,

when the cell image processing system is in a common mode, the display output device is configured to output one image with highest definition in images with different focusing depths of the cells to the display interface;

when the cell image processing system is in a depth of field mode, the display output device is configured to output a plurality of images of different in-focus depths of the cell to the display interface.

The cellular image processing system according to any one of claims 1 to 3,

the display output device is further configured to simultaneously output a plurality of images of different in-focus depths of cells in the at least one field of view, or,

dynamically outputting a plurality of images of different in-focus depths of cells in the at least one field of view.

The cellular image processing system according to any one of claims 1 to 4, wherein the display output device is further configured to:

when the shot cells are judged to be normal cells, outputting images with highest definition shot for the normal cells to the display interface;

and when the shot cells are judged to be abnormal cells, outputting images of different focusing depths of the abnormal cells to the display interface.

The cellular image processing system according to claim 1, wherein the control device is configured to set a range of focusing depths, or,

is configured to set a difference in focus depth of images of different focus depths of the at least one field of view, the difference in focus depth of each two adjacent images among the images of different focus depths being the same or different.

The cellular image processing system according to claim 1,

the display output device is further configured to:

when the diameter or the projection area of the shot cell exceeds a first preset threshold value, outputting images of the cell with different focusing depths;

and when the diameter or the projection area of the shot cell is smaller than a second preset threshold value, outputting the image with the highest definition shot for the cell.

The cellular image processing system according to any one of claims 1 to 7, wherein the cellular image processing system further includes:

an automatic placement device configured to receive the smear and to transport the smear to an imaging position of the lens assembly.

The cellular image processing system according to any one of claims 1 to 8, wherein the display output device is further configured to output the image with the highest resolution corresponding to the selected region when the partial region of one image of the cellular images of different in-focus depths of the at least one field of view is selected.

The cellular image processing system according to any one of claims 1 to 9,

the display output device is further configured to switch and output images of different focal depths of cells in a selected visual field, wherein the selected visual field is a visual field selected by a user in different visual fields of the smear to be shot or a visual field selected by the cell image processing system according to a preset rule.

The cellular image processing system according to claim 10,

the display output device is further configured to switch and output images with different focusing depths of the cells in the selected visual field according to human-computer interaction operation.

The cellular image processing system according to claim 10,

the display output device is also configured to automatically switch and output images with different focusing depths of cells in the selected visual field according to a preset switching speed or a preset switching time.

The cellular image processing system according to any one of claims 1 to 12,

the display output device is further configured to output a registered image of different in-focus depth images of the captured cell.

The cellular image processing system according to claim 1,

the display output device comprises a display output interface configured to output a signal corresponding to the image to an external device.

The cellular image processing system according to claim 14,

the display output device also comprises a display device which is connected with the display output interface and is configured to receive the signal output by the display output interface and correspondingly display an image.

A cell image processing method applied to an image processing system includes:

adjusting the relative position of the digital imaging device and the smear;

in response to parallel-direction movement of a digital camera relative to the plane of the smear, taking images of cells in respective fields of view through different fields of view in the smear formed by a lens group;

taking images of different depths of focus of cells in at least one of the different fields of view formed by the lens group in response to vertical movement of the digital camera relative to the plane of the smear;

outputting the different fields of view cell images to a display interface, wherein the different fields of view cell images include: a cellular image of different in-focus depths of at least one of the different fields of view.

The method of claim 16, wherein the cellular image processing system includes a normal mode and a depth of field mode, wherein,

when the cell image processing system is in a common mode, outputting an image with highest definition in images with different focusing depths of the photographed cell to the display interface;

and when the cell image processing system is in the depth of field mode, outputting a plurality of images of at least one photographed cell with different focusing depths to the display interface.

The method of any of claims 16-17, wherein said outputting to a display interface images of different in-focus depths of cells in at least one of the different fields of view comprises:

simultaneously outputting a plurality of images of cells in at least one field of view at different depths of focus, or,

a plurality of images of different in-focus depths of cells in at least one field of view are dynamically output.

The method according to any one of claims 16-18, wherein the method comprises:

identifying whether a cell in the different field of view is abnormal;

when the shot cells are recognized to be normal cells, outputting images with highest definition shot for the normal cells to the display interface;

and when the shot cells are identified to be abnormal cells, outputting images of the abnormal cells with different focusing depths to the display interface.

The method of claim 16, wherein the method further comprises:

the range of the focusing depth is set, or,

setting a difference in focus depth of images of different focus depths of the at least one field of view, the difference in focus depth of each two adjacent images among the images of different focus depths being the same or different.

The method of claim 16, wherein the method further comprises:

recognizing the morphology of the cell;

when the diameter or the projection area presented by the cell exceeds a first preset threshold value, outputting images of the cell with different focusing depths;

and when the diameter or the projection area of the cell is smaller than a second preset threshold value, outputting the image with the highest definition shot on the cell.

The method according to any one of claims 16-21, wherein the method further comprises:

and before the relative position of the digital imaging device and the smear is adjusted, conveying the smear to the imaging position of the lens group.

The method of claim 16, wherein the method further comprises:

and outputting the image with the highest definition corresponding to the selected area when the partial area of one image of the cell images with different focusing depths of the at least one field of view is selected.

The method of any of claims 16-23, wherein said outputting to a display interface images of different in-focus depths of cells in at least one of the different fields of view comprises:

when at least one selected field exists in different fields of the smear, switching to output images of different in-focus depths of cells in the selected at least one field; the at least one selected field of view is a field of view selected by a user among different fields of view in which the smear is taken or a field of view selected by the cellular image processing system according to a preset rule.

The method of claim 24, wherein said switching outputs images of different in-focus depths of cells in the selected at least one field of view, comprising:

switching and outputting images of different focusing depths of cells in the selected at least one field of view according to the man-machine interaction operation; or

And automatically switching and outputting images of different focusing depths of the cells in the selected at least one field of view according to the preset switching speed or the preset switching time.

The method of any of claims 16-25, wherein said outputting to a display interface images of different in-focus depths of cells in at least one of the different fields of view comprises:

when there is at least one selected field of view in the different fields of view of the smear, outputting a registered image of the different in-focus depth images of the at least one selected field of view.

An automatic film reading apparatus comprising:

the receiving device is configured to receive images of different focusing depths of cells in at least one visual field acquired when the digital imaging device shoots the smear;

a display device configured to output to a display interface images of different in-focus depths of cells in at least one of the different fields of view.

The automatic film reading device according to claim 27, wherein the automatic film reading device comprises a normal mode and a depth of view mode,

when the automatic film reading device is in a common mode, the display device is configured to output one image with the highest definition in images with different focusing depths of cells to the display interface;

when the automatic film reading device is in a depth of field mode, the display device is configured to output a plurality of cell images of cells with different focusing depths to the display interface.

The automatic film reading apparatus according to claim 28,

the display device further configured to simultaneously output a plurality of images of different in-focus depths of cells in at least one of the different fields of view, or,

dynamically outputting a plurality of images of different in-focus depths of cells in at least one of the different fields of view.

The automatic film reading apparatus according to claim 29,

the display device is further configured to:

when the cell in at least one of the different visual fields is judged to be a normal cell, outputting an image with the highest definition for shooting the normal cell to the display interface;

when the cell in at least one of the different visual fields is judged to be an abnormal cell, outputting images of the abnormal cell with different focusing depths to the display interface.

The automatic sheet reading apparatus according to any one of claims 27 to 30,

the display device is further configured to:

when the diameter or the projection area presented by the cell in at least one of the different visual fields is judged to exceed a first preset threshold value, outputting the images of the cell with different focusing depths;

and when the diameter or the projection area of the cell in at least one of the different visual fields is judged to be smaller than a second preset threshold value, outputting the image with the highest definition shot for the cell.

The automatic sheet reading apparatus according to any one of claims 27 to 31,

and the display device is also configured to output images of different focusing depths of cells in the selected visual field according to human-computer interaction operation or automatic switching when the selected visual field exists in different visual fields of the smear.

A cell image processing system, the cell image processing system comprising:

a memory configured to store executable instructions;

a processor configured to execute the executable instructions stored in the memory to perform the method of cell image processing according to any one of claims 16 to 26.

A storage medium storing executable instructions configured to cause a processor to perform the method of cell image processing of any one of claims 16 to 26 when the executable instructions are executed.