阅读说明：本技术 一种人胎盘干细胞提取物冻干粉及其制备方法 (Human placenta stem cell extract freeze-dried powder and preparation method thereof ) 是由 赵岩 苏波 武凯 于 2020-06-20 设计创作，主要内容包括：本发明公开了一种人胎盘干细胞提取物冻干粉及其制备方法,尤其是一种人胎盘干细胞提取物冻干粉的制备方法,包括如下步骤：S1使用PH值5.6-6.8、含血清的细胞培养基培养人胎盘间充质干细胞至70-90%融合时,加入去离子水,稳定剂、赋形剂混合溶解至透明,得到混合溶液；S2将混合溶液在PH值5.6-6.8的环境下静置,静置后通过高温消毒杀菌；S3将高温消毒杀菌后的混合溶液进行降温处理,使得温度降至2-5度；S4采用精密滤纸进行过滤。本发明的人胎盘干细胞冻干粉对老化的机体有全面调节、恢复年轻之功效；它能大幅度提高机体代谢水平,增强生理机能,提高机体免疫力,延长青春期,美化肌肤,增长寿命,延缓衰老,同时,冻干粉储存期长,不易降解,使用方便。(The invention discloses a human placenta stem cell extract freeze-dried powder and a preparation method thereof, in particular to a preparation method of a human placenta stem cell extract freeze-dried powder, which comprises the following steps: s1, culturing human placenta mesenchymal stem cells by using a cell culture medium containing serum with a pH value of 5.6-6.8 until 70-90% of the cells are fused, adding deionized water, a stabilizer and an excipient, mixing and dissolving until the mixture is transparent to obtain a mixed solution; s2, standing the mixed solution in an environment with a pH value of 5.6-6.8, and sterilizing at high temperature after standing; s3, cooling the mixed solution after high-temperature disinfection and sterilization to 2-5 ℃; s4 filtration was performed using precision filter paper. The human placental stem cell freeze-dried powder has the effects of comprehensively regulating an aged organism and recovering young; it can greatly raise metabolism level of organism, raise physiological function, raise immunity of organism, prolong puberty period, beautify skin, prolong life and delay senility, and at the same time, the freeze-dried powder is long in storage period, not easy to degrade and convenient for use.)

1. A preparation method of human placental stem cell extract freeze-dried powder is characterized by comprising the following steps:

s1, culturing human placenta mesenchymal stem cells by using a cell culture medium containing serum with a pH value of 5.6-6.8 until 70-90% of the cells are fused, adding deionized water, a stabilizer and an excipient, mixing and dissolving until the mixture is transparent to obtain a mixed solution;

s2, standing the mixed solution in an environment with a pH value of 5.6-6.8, and sterilizing at high temperature after standing;

s3, cooling the mixed solution after high-temperature disinfection and sterilization to 2-5 ℃;

s4, filtering by using precision filter paper;

s5 filling the solution after precision filtration into a bottle, covering the bottle stopper, filling into a freeze-drying device, standing for 60 minutes, defoaming, and grinding to obtain the human placenta stem cell extract freeze-dried powder.

2. The method for preparing lyophilized powder of human placental stem cell extract according to claim 1, wherein the room temperature is maintained at 20-25 degrees during the preparation.

3. The method for preparing lyophilized powder of human placental stem cell extract according to claim 1, wherein the cell culture medium is DMEM/F12 culture medium, and the culture medium contains antibacterial antibiotics.

4. The method of claim 1, wherein the precision filter paper of step S4 is a filter membrane with a pore size of 0.45 μm.

5. A lyophilized powder of human placental stem cell extract comprising the lyophilized powder obtained by the preparation method of claims 1 to 4.

Technical Field

The invention relates to the technical field of freeze-dried powder preparation, in particular to human placenta stem cell extract freeze-dried powder and a preparation method thereof.

Background

The human placenta stem cell extract is freeze dried powder prepared with human placenta stem cell cultured to secrete bioactive matters including protein, polypeptide, cell growth factor, bioactive factor and other components and through special separation and extraction process and processing. The multiple active components in the human placental stem cell extract have complex physiological effects, each component can affect a plurality of tissues and organs, and the improvement of the physiological function of each form is not the effect of a single factor but the mutual coordination effect of a plurality of factors is needed, so the human placental stem cell extract has better effects of resisting aging and activating the body functions than the single cytokine. The placenta stem cell extract has effects of caring skin, resisting aging, activating organism function, regenerating cell, repairing ultraviolet injury, and improving skin immunity.

Disclosure of Invention

Based on the technical problems in the background art, the invention provides a human placental stem cell extract freeze-dried powder and a preparation method thereof.

The invention provides a human placenta stem cell extract freeze-dried powder and a preparation method thereof, comprising the following steps:

s1, culturing human placenta mesenchymal stem cells by using a cell culture medium containing serum with a pH value of 5.6-6.8 until 70-90% of the cells are fused, adding deionized water, a stabilizer and an excipient, mixing and dissolving until the mixture is transparent to obtain a mixed solution;

s2, standing the mixed solution in an environment with a pH value of 5.6-6.8, and sterilizing at high temperature after standing;

s3, cooling the mixed solution after high-temperature disinfection and sterilization to 2-5 ℃;

s4, filtering by using precision filter paper;

s5 filling the solution after precision filtration into a bottle, covering the bottle stopper, filling into a freeze-drying device, standing for 60 minutes, defoaming, and grinding to obtain the human placenta stem cell extract freeze-dried powder.

Preferably, the room temperature is maintained at 20 to 25 degrees during the preparation.

Preferably, the cell culture medium is DMEM/F12 medium, and the nutrient medium contains antibacterial antibiotics.

Preferably, the precision filter paper of step S4 is a filter membrane with a pore size of 0.45 μm.

A freeze-dried powder of human placental stem cell extract comprises the freeze-dried powder obtained by the preparation method.

In the invention, the human placental stem cell extract freeze-dried powder contains various life active substances, has extremely high excitation effect on cell division and proliferation of an organism, and has the effects of comprehensively regulating an aged organism and recovering young; the placenta stem cell extract freeze-dried powder prepared by the special process has the advantages of long storage period, difficult degradation and convenient use.

The human placental stem cell freeze-dried powder has the effects of comprehensively regulating an aged organism and recovering young; it can greatly raise metabolism level of organism, raise physiological function, raise immunity of organism, prolong puberty period, beautify skin, prolong life and delay senility, and at the same time, the freeze-dried powder is long in storage period, not easy to degrade and convenient for use.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.