阅读说明：本技术 用于非核糖体肽合成酶的组装和修饰的系统 (System for assembly and modification of non-ribosomal peptide synthetases ) 是由 凯南·波祖尤克 安娜贝尔·林克 海尔格·波特 于 2019-01-15 设计创作，主要内容包括：本发明涉及用于非核糖体肽合成酶(NRPS)的组装和修饰的系统。该系统使用了包含缩合亚结构域的新颖的定义明确的构建基块(单元)。该策略允许有效地组合被称为交换单元(XU<Sub>2.0</Sub>)的组装单元,而无需考虑其对后续NRPS腺苷酸化结构域的天然存在的特异性。本发明的系统允许容易地组装具有选择的任何氨基酸序列的NRPS,而没有由于天然存在的NRPS单元而引起的任何限制。该系统还允许使用创造性的XU<Sub>2.0</Sub>交换天然NRPS构建基块,从而产生经修饰的肽。本发明提供了所述系统、它们的单个交换单元、编码这些单元的核酸、及其方法和用途。(The present invention relates to a system for the assembly and modification of non-ribosomal peptide synthetases (NRPS). The system uses novel well-defined building blocks (units) comprising condensation subdomains. This strategy allows to efficiently combine units called switching units (XU) 2.0 ) Without regard to its naturally occurring specificity for a subsequent NRPS adenylation domain. The system of the invention allows easy assembly of NRPS with any amino acid sequence selected without any limitations due to naturally occurring NRPS units. The system also allowsPermitting use of the inventive XU 2.0 The native NRPS building blocks were exchanged, resulting in a modified peptide. The invention provides such systems, their individual exchange units, nucleic acids encoding these units, and methods and uses thereof.)