阅读说明：本技术 一种抗菌棉麻织物的制备方法 (Preparation method of antibacterial cotton-linen fabric ) 是由 葛彦 傅海洪 汤佳鹏 赵士友 张秋娴 于 2020-06-01 设计创作，主要内容包括：本发明涉及纺织面料加工技术领域,公开了一种抗菌棉麻织物的制备方法,制备方法包括：将棉麻织物经碳酸钠溶液水煮处理,再经等离子处理后,浸渍在含有单萜醇、β-葡萄糖苷酶的反应液中反应使单萜醇接枝到纤维素上,之后水洗烘干制得抗菌棉麻织物。本发明制备得到的棉麻织物具有长效耐洗抗菌作用。(The invention relates to the technical field of textile fabric processing, and discloses a preparation method of an antibacterial cotton-linen fabric, which comprises the following steps: the antibacterial cotton and linen fabric is boiled in sodium carbonate solution, is subjected to plasma treatment, is soaked in a reaction solution containing monoterpene alcohol and beta-glucosidase to react, so that the monoterpene alcohol is grafted onto cellulose, and is washed with water and dried to obtain the antibacterial cotton and linen fabric. The cotton and linen fabric prepared by the invention has long-acting wash-resistant and antibacterial effects.)

1. The preparation method of the antibacterial cotton-linen fabric is characterized by comprising the following steps:

s1, boiling cotton and linen fabrics with sodium carbonate solution, washing with water, drying, and treating with plasma;

s2, adding monoterpene alcohol and beta-glucosidase into a buffer solution, and fully stirring to obtain a reaction solution; the buffer is a 100mM phosphate citrate buffer pH5.0 containing 30% (v/v) acetone;

s3, soaking the cotton and linen fabric processed in the step S1 in the reaction liquid prepared in the step S2, reacting at 37-45 ℃ for 12-24 hours, adding a sodium carbonate solution with the concentration of 20-50g/L to stop the reaction, taking out the cotton and linen fabric, washing with water, and drying to obtain the antibacterial cotton and linen fabric; the volume ratio of the reaction liquid to the sodium carbonate solution is 1: 2-3.

2. The method for preparing an antibacterial linen-cotton fabric according to claim 1, wherein in step S1, the concentration of the sodium carbonate solution is 20-50g/L, the dosage ratio of the sodium carbonate solution to the linen-cotton fabric is (8-10) L:1kg, and the water boiling time is 20-30 min.

3. The method for preparing an antibacterial linen-cotton fabric according to claim 1, wherein in step S1, the plasma treatment conditions are as follows: the gas adopts oxygen, the treatment power is 100-300W, the pressure is 50-60Pa, and the treatment time is 20-30 min.

4. The method for preparing an antibacterial linen cotton fabric according to claim 1, wherein in step S2, the dosage ratio of monoterpene alcohol, beta-glucosidase and buffer is (0.6-1.2) g (8-10) g:100 mL.

5. The method of claim 1, wherein in step S2, the monoterpene alcohol is one of geraniol, nerol, terpineol, and citronellol.

6. The method of claim 1, wherein in step S2, the β -glucosidase is one of almond β -glucosidase, aspergillus niger β -glucosidase, trichoderma reesei β -glucosidase, and candida morganii β -glucosidase.

7. The method for preparing an antibacterial linen cotton fabric according to claim 1, wherein in step S2, the specific activity of the beta-glucosidase is 45-80U/mg.

8. The method for preparing an antibacterial linen-cotton fabric according to claim 1, wherein in step S3, the bath ratio of the linen-cotton fabric in the reaction solution is 1: 30-50.

Technical Field

The invention relates to the technical field of textile fabric processing, in particular to a preparation method of an antibacterial cotton-linen fabric.

Background

Monoterpene alcohol is an alcohol derivative of monoterpene, has hydroxyl group and stronger hydrophilicity than terpenes, and has biological activity similar to terpenes. Among monoterpene alcohols, geraniol, nerol, terpineol, and citronellol are representative substances having antibacterial activity. The minimum inhibitory concentration of geraniol on Trichophyton mentagrophytes and Microsporum audum is 0.39 mg/mL; nerol has the best antibacterial effect on phytophthora capsici, and EC₅₀32.7668 mg/L; the minimum inhibitory concentration and the minimum bactericidal concentration of the terpineol to the escherichia coli are both 0.78 mu l/mL; citronellol has the activity of inhibiting staphylococcus aureus and typhoid bacillus.

Generally, terpenoids are hydrocarbons and have no active groups, so that the terpenoids cannot be normally grafted onto fiber fabrics and can only be adsorbed on the surfaces of the fabrics through small-particle-size materials such as microcapsules, and the adsorption state is unstable, so that the washability of the fabrics is poor, and the antibacterial effect of the fabrics is not ideal. Therefore, there is a need to develop a method for firmly grafting terpenoids on fabrics and fully exerting the powerful antibacterial ability thereof so as to prepare fabrics with long-lasting wash-resistant antibacterial effect.

Disclosure of Invention

In view of the above, the invention aims to provide a preparation method of an antibacterial cotton-linen fabric, and the cotton-linen fabric obtained by the preparation method has a long-acting wash-resistant antibacterial effect.

In order to solve the technical problem, the invention provides a preparation method of an antibacterial cotton-linen fabric, which comprises the following steps:

s1, boiling cotton and linen fabrics with sodium carbonate solution, washing with water, drying, and treating with plasma;

s2, preparing a reaction solution: adding monoterpene alcohol and beta-glucosidase into the buffer solution, and fully stirring to obtain a reaction solution; the buffer is a 100mM phosphate citrate buffer pH5.0 containing 30% (v/v) acetone;

s3, soaking the cotton and linen fabric processed in the step S1 in the reaction liquid prepared in the step S2, reacting at 37-45 ℃ for 12-24 hours, adding a sodium carbonate solution with the concentration of 20-50g/L to stop the reaction, taking out the cotton and linen fabric, washing with water, and drying to obtain the antibacterial cotton and linen fabric; in the step, the volume ratio of the reaction liquid to the sodium carbonate solution is 1: 2-3.

Preferably, in step S1, the concentration of the sodium carbonate solution is 20-50g/L, the dosage ratio of the sodium carbonate solution to the cotton-flax fabrics is (8-10) L:1kg, and the water boiling time is 20-30 min.

Preferably, in step S1, the plasma processing conditions are: the gas adopts oxygen, the treatment power is 100-300W, the pressure is 50-60Pa, and the treatment time is 20-30 min.

Preferably, in step S2, the ratio of the amount of monoterpene alcohol to the amount of β -glucosidase to the amount of buffer is (0.6-1.2) g (8-10) g:100 mL.

Preferably, in step S2, the monoterpene alcohol is one of geraniol, nerol, terpineol, and citronellol.

Preferably, in step S2, the β -glucosidase is one of amygdala β -glucosidase, aspergillus niger β -glucosidase, trichoderma reesei β -glucosidase and candida morganii β -glucosidase.

Preferably, in step S2, the specific activity of the beta-glucosidase is 45-80U/mg.

Preferably, in step S3, the bath ratio of the cotton and linen fabric treated in step S1 in the reaction solution is 1: 30-50.

Compared with the prior art, the invention has the following beneficial effects:

the invention soaks the cotton-flax fabric after preconditioning in the reaction liquid prepared to prepare the antibacterial cotton-flax fabric, beta-glucosidase in the reaction liquid has activity of synthetic glycosidic bond, so can be under the condition without activating glycosyl, the monoterpene alcohol is grafted to the cellulose molecule of the cotton-flax fabric through the synthetic glycosidic bond directly, and then the antibacterial cotton-flax fabric prepared can utilize hydrophobic end of its grafted monoterpene alcohol to destroy the cell membrane of the microorganism, achieve the goal of denaturating protein and contact microbicide, the acetone in the reaction liquid has effects similar to surfactant, can bring hydrophobic monoterpene alcohol into water by utilizing acetone, can avoid leading into the surfactant and leading to lead to beta-glucosidase hydration layer to destroy and inactivate, make the cotton-flax fabric have long-term washing-resistant antibacterial action. The preparation method has simple process and strong operability.

Detailed Description

The invention will be better understood from the following examples. However, those skilled in the art will readily appreciate that the description of the embodiments is only for illustrating the present invention and should not be taken as limiting the invention as detailed in the claims.

And (3) specific activity determination of beta-glucosidase: the measurement was carried out by using a beta-glucosidase measurement kit and a BCA protein content measurement kit of Solebao corporation in accordance with the procedures of the specification.

Determining the content of monoterpene alcohol: measuring the content of the monoterpene alcohol component by a GC-MS method, wherein the sample injection amount is as follows: 1.0 μ L, no split injection; the temperature of a sample inlet is 250 ℃; carrier gas: high-purity nitrogen (more than or equal to 99.999%), flow: 1.0 mL/min; HP-5MS capillary chromatography column (30m × 0.32mm, 0.25 μm); the column temperature program was as follows: the initial temperature is 50 deg.C, held for 2min, increased to 90 deg.C at 10 deg.C/min, increased to 130 deg.C at 5 deg.C/min, increased to 240 deg.C at 20 deg.C/min, and held for 1 min. Mass spectrum conditions: EI ion source, electron energy 70 eV; ion source temperature: 230 ℃; scanning range: 50-500 amu. SIM mode, monitoring ions including: m/z121, 123, 136 and 138, wherein the characteristic ions of nerol, geraniol and terpineol are m/z121 and 136, and the characteristic ions of citronellol are m/z123 and 138.