阅读说明：本技术 涉及突变体难辨梭菌毒素的组合物及其方法 (Compositions and methods relating to mutant clostridium difficile toxins ) 是由 M·K·希胡 A·S·安德森 R·G·K·唐纳德 K·U·扬森 N·K·卡利安 T·L·米 于 2012-04-20 设计创作，主要内容包括：在一方面,本发明涉及免疫原性组合物,其包含突变体难辨梭菌毒素A和/或突变体难辨梭菌毒素B。相对于相应的野生型难辨梭菌毒素,每种突变体毒素包括具有至少一个突变的葡萄糖基转移酶结构域和具有至少一个突变的半胱氨酸蛋白酶结构域。所述突变体毒素还可包含至少一个经化学交联的氨基酸。在另一方面,本发明涉及结合所述免疫原性组合物的抗体或其结合片段。在另外的方面,本发明涉及编码任意前述物质的分离的核苷酸序列,以及使用任意前述组合物的方法。(In one aspect, the invention relates to an immunogenic composition comprising a mutant clostridium difficile toxin a and/or a mutant clostridium difficile toxin B. Each mutant toxin includes a glucosyltransferase domain having at least one mutation and a cysteine protease domain having at least one mutation relative to the corresponding wild-type clostridium difficile toxin. The mutant toxin may also comprise at least one chemically cross-linked amino acid. In another aspect, the invention relates to antibodies or binding fragments thereof that bind to the immunogenic composition. In further aspects, the invention relates to isolated nucleotide sequences encoding any of the foregoing, as well as methods of using any of the foregoing compositions.)