阅读说明：本技术 一种含糖超支化聚合物及其制备方法和应用 (Sugar-containing hyperbranched polymer and preparation method and application thereof ) 是由 朱韵 李果 廖明佳 于 2020-06-09 设计创作，主要内容包括：本发明公开了一种含糖超支化聚合物的制备方法,步骤为：将1-(2-氨基乙基)哌嗪、聚乙二醇丙烯酸酯和氨基葡萄糖盐酸盐在水中完全溶解,在惰性气体氛围下,加入三乙胺,避光,60℃～70℃下,反应3～5天,在丙酮中沉淀,烘干,得到含糖超支化聚合物,所述的1-(2-氨基乙基)哌嗪和聚乙二醇丙烯酸酯的摩尔比为2:3。该含糖超支化聚合物以叔胺为支化单元,具有自发荧光性。含糖超支化聚合物的这种荧光性质将省去后续细菌测试的荧光标记程序。含糖超支化聚合物能通过表面的葡萄糖靶向识别并粘附大肠杆菌,对大肠杆菌具有靶向选择性。(The invention discloses a preparation method of a sugar-containing hyperbranched polymer, which comprises the following steps: completely dissolving 1- (2-aminoethyl) piperazine, polyethylene glycol acrylate and glucosamine hydrochloride in water, adding triethylamine in an inert gas atmosphere, keeping out of the sun, reacting for 3-5 days at 60-70 ℃, precipitating in acetone, and drying to obtain the sugar-containing hyperbranched polymer, wherein the molar ratio of the 1- (2-aminoethyl) piperazine to the polyethylene glycol acrylate is 2: 3. The sugar-containing hyperbranched polymer takes tertiary amine as a branching unit and has autofluorescence. This fluorescent property of the sugar-containing hyperbranched polymer will eliminate the fluorescent labeling procedure for subsequent bacterial testing. The sugar-containing hyperbranched polymer can be identified and adhered to escherichia coli in a targeted manner through glucose on the surface, and has targeted selectivity on escherichia coli.)

1. A preparation method of a sugar-containing hyperbranched polymer is characterized by comprising the following steps: completely dissolving 1- (2-aminoethyl) piperazine, polyethylene glycol acrylate and glucosamine hydrochloride in water, adding triethylamine in an inert gas atmosphere, keeping out of the sun, reacting for 3-5 days at 60-70 ℃, precipitating in acetone, and drying to obtain the sugar-containing hyperbranched polymer, wherein the molar ratio of the 1- (2-aminoethyl) piperazine to the polyethylene glycol acrylate is 2: 3.

2. The method for preparing a hyperbranched sugar-containing polymer according to claim 1, wherein the number of moles of glucosamine hydrochloride is greater than 2 times the number of moles of polyethylene glycol acrylate.

3. The sugar-containing hyperbranched polymer obtained by the method for preparing the sugar-containing hyperbranched polymer according to claim 1.

4. Use of a hyperbranched sugar-containing polymer according to claim 3 for fluorescent labeling of bacteria.

5. The use of a hyperbranched sugar-containing polymer according to claim 4 for fluorescence labeling of bacteria, wherein said bacteria is Escherichia coli.

Technical Field

The invention belongs to the field of fluorescent labeling materials, and relates to a sugar-containing hyperbranched polymer, and a preparation method and application thereof.

Background

The degree of branching of the hyperbranched polymer may reflect the flexibility of the branched portion of the structure as well as the intrinsic viscosity of the polymer. The higher the degree of branching, the lower the viscosity of the hyperbranched polymer, which in turn also affects the relative solubility of the polymer in various media. Compared with linear macromolecules with similar molecular weight, the solubility of the hyperbranched polymer is also greatly improved. In addition, since a great amount of modifiable terminal functional groups exist on the surface of the polymer, if the reactive groups are reserved, the hyperbranched polymer also has high chemical reactivity, and various functions can be endowed by modifying the terminal functional groups. Besides the three characteristics of low viscosity, high solubility and multiple functionality, the hyperbranched polymer also has good fluidity and is easy to form a film, and a nonpolar inner layer structure can be combined with another polar outer layer structure. Hyperbranched polymers have gained attention over the last 30 years due to their unique chemical-physical properties and their potential applications in coatings, additives, drug delivery, macromolecular building blocks, nanotechnology and supramolecular science.

How the tertiary amine in the material maintains its high fluorescence efficiency is critical to successful fluorescence labeling using this chromophore. It has been shown that diamond emits intense fluorescence around 700nm at an excitation wavelength of 514nm due to the presence of nitrogen vacancy defects. Like the nitrogen vacancies in diamond, the hyperbranched polymer of aliphatic tertiary amine immobilizes the tertiary amine groups in the polymer network, reducing the collision relaxation rate and improving the fluorescence quantum yield. Therefore, the tertiary amine can show stronger fluorescence as a branching unit of the hyperbranched polymer, and the fluorescence efficiency is continuously improved with the increase of the molecular weight.

Therefore, the preparation of the sugar-containing hyperbranched polymer taking the tertiary amine as the branching unit has the property of autofluorescence, and the fluorescence labeling procedure can be omitted when the bacterial activity is detected.

Disclosure of Invention

In view of the above, the present invention aims to provide a sugar-containing hyperbranched polymer, and a preparation method and applications thereof. In order to achieve the purpose, the invention provides the following technical scheme:

1. a preparation method of sugar-containing hyperbranched polymer comprises the following steps: completely dissolving 1- (2-aminoethyl) piperazine, polyethylene glycol acrylate and glucosamine hydrochloride in water, adding triethylamine in an inert gas atmosphere, keeping out of the sun, reacting for 3-5 days at 60-70 ℃, precipitating in acetone, and drying to obtain the sugar-containing hyperbranched polymer, wherein the molar ratio of the 1- (2-aminoethyl) piperazine to the polyethylene glycol acrylate is 2: 3.

Further, the mole number of the glucosamine hydrochloride is more than 2 times of that of the polyethylene glycol acrylate.

2. The sugar-containing hyperbranched polymer is obtained by the preparation method of the sugar-containing hyperbranched polymer.

3. The application of the sugar-containing hyperbranched polymer in fluorescence labeling of bacteria is provided.

Further, the bacterium is escherichia coli.

The invention has the beneficial effects that: the invention successfully synthesizes the sugar-containing hyperbranched polymer through one-pot reaction of three monomers, namely polyethylene glycol acrylate, 1- (2-aminoethyl) piperazine and glucosamine. The sugar-containing hyperbranched polymer takes tertiary amine as a branching unit and has autofluorescence. This fluorescent property of the sugar-containing hyperbranched polymer will eliminate the fluorescent labeling procedure for subsequent bacterial testing. The sugar-containing hyperbranched polymer can be identified and adhered to escherichia coli in a targeted manner through glucose on the surface, and has targeted selectivity on escherichia coli.

Drawings

In order to make the object, technical scheme and beneficial effect of the invention more clear, the invention provides the following drawings for explanation:

FIG. 1 shows a synthetic route of sugar-containing hyperbranched polymer.

FIG. 2 is a confocal image of a sugar-containing hyperbranched polymer co-cultured with E.coli.

Detailed Description

Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.