阅读说明：本技术 (s)-3-硫代吗啉甲酸促进脐带间充质干细胞增殖的用途、脐带间充质干细胞培养基 (Application of (S) -3-thiomorpholine formic acid in promoting proliferation of umbilical cord mesenchymal stem cells and umbilical cord mesenchymal stem cell culture medium ) 是由 于海涛 杨晓旭 李响 于 2020-05-05 设计创作，主要内容包括：本发明公开了(S)-3-硫代吗啉甲酸促进脐带间充质干细胞增殖的用途、脐带间充质干细胞培养基。本发明发现,(S)-3-硫代吗啉甲酸具有促进UC-MSCs增殖的作用,在培养基中添加适量(S)-3-硫代吗啉甲酸可以显著提高UC-MSCs的增殖率,且培养增殖后的UC-MSCs依然具有高纯度,不会被诱导分化。(S)-3-硫代吗啉甲酸促进UC-MSCs增殖的作用可能与Notch1通路有关。(S)-3-硫代吗啉甲酸具有开发成脐带间充质干细胞体外增殖培养基的前景。(The invention discloses application of (S) -3-thiomorpholine formic acid in promoting proliferation of umbilical cord mesenchymal stem cells and an umbilical cord mesenchymal stem cell culture medium. The invention discovers that (S) -3-thiomorpholine formic acid has the effect of promoting the proliferation of UC-MSCs, the proliferation rate of the UC-MSCs can be obviously improved by adding a proper amount of (S) -3-thiomorpholine formic acid into a culture medium, and the UC-MSCs after being cultured and proliferated still have high purity and cannot be induced to differentiate. The effect of (S) -3-thiomorpholinoic acid on promoting proliferation of UC-MSCs may be related to the Notch1 pathway. The (S) -3-thiomorpholine formic acid has the prospect of being developed into an in-vitro proliferation culture medium of umbilical cord mesenchymal stem cells.)

1. Use of (S) -3-thiomorpholine carboxylic acid for promoting proliferation of umbilical cord mesenchymal stem cells in vitro.

2. An application of (S) -3-thiomorpholine formic acid in preparing the in-vitro proliferation culture medium of umbilical cord mesenchymal stem cells.

3. An in vitro proliferation culture medium of umbilical cord mesenchymal stem cells, which is characterized in that: contains (S) -3-thiomorpholine carboxylic acid.

4. An in vitro proliferation medium according to claim 3, characterized in that: and fetal bovine serum.

Technical Field

The invention relates to the field of stem cells, in particular to application of (S) -3-thiomorpholine formic acid in promoting proliferation of umbilical cord mesenchymal stem cells in vitro and an umbilical cord mesenchymal stem cell culture medium.

Background

Mesenchymal Stem Cells (MSCs) are currently the most promising seed cells. MSCs, a class of undifferentiated cells with self-renewal and pluripotency, have been shown to have a strong ability to repair regenerated damaged tissues due to their self-renewal, replication and pluripotency. The main mechanisms of action of MSCs include: migrating and planting in the damaged part, differentiating and regenerating damaged tissue cells, secreting cell growth factor, nourishing local tissue, stimulating the activation of endogenous stem cells, secreting immunoregulation and inflammation inhibiting factor, reducing local inflammatory reaction, protecting tissue cells and the like.

Among the various MSCs, umbilical cord mesenchymal stem cells (UC-MSCs) are again a more desirable choice: compared with the mesenchymal stem cells of the marrow, the mesenchymal stem cells of the umbilical cord have simple material taking and quick self-renewal; compared with the embryonic stem cells, the source of the umbilical cord mesenchymal stem cells is not ethically controversial and does not relate to ethical problems.

An important aspect for promoting the application of the umbilical cord mesenchymal stem cells is how to promote the rapid expansion of the umbilical cord mesenchymal stem cells in vitro.

The Notch signaling pathway consists of a Notch receptor, a Notch ligand (DSL protein), transcription factor CSL (a class of DNA binding proteins) and downstream effectors. The existing research shows that the Notch signaling pathway has an important regulation function in promoting cell proliferation, and the Notch1 has a function of promoting the cell proliferation of UC-MSCs after the expression is up-regulated.

Disclosure of Invention

The invention aims to provide application of (S) -3-thiomorpholine formic acid in promoting proliferation of umbilical cord mesenchymal stem cells in vitro and a culture medium for promoting the proliferation of the umbilical cord mesenchymal stem cells in vitro.

The technical scheme for realizing the purpose is as follows:

use of (S) -3-thiomorpholine carboxylic acid for promoting proliferation of umbilical cord mesenchymal stem cells in vitro.

An application of (S) -3-thiomorpholine formic acid in preparing the in-vitro proliferation culture medium of umbilical cord mesenchymal stem cells.

An in vitro proliferation culture medium for umbilical cord mesenchymal stem cells contains (S) -3-thiomorpholinoic acid.

Further, it contains fetal bovine serum and the like.

Has the advantages that:

the invention discovers that (S) -3-thiomorpholine formic acid has the effect of promoting the proliferation of UC-MSCs, the proliferation rate of the UC-MSCs can be obviously improved by adding a proper amount of (S) -3-thiomorpholine formic acid into a culture medium, and the UC-MSCs after being cultured and proliferated still have high purity and cannot be induced to differentiate. The effect of (S) -3-thiomorpholinoic acid on promoting proliferation of UC-MSCs may be related to the Notch1 pathway. The (S) -3-thiomorpholine formic acid has the prospect of being developed into an in-vitro proliferation culture medium of umbilical cord mesenchymal stem cells.

Drawings

FIG. 1 shows the flow-type identification results of UC-MSCs of generation 5;

FIG. 2 shows the in vitro proliferation rate of UC-MSCs in each group;

FIG. 3 shows the result of Western Blot assay.

Detailed Description