阅读说明：本技术 一种热处理辅助酶法制备玉米肽的制备方法 (Preparation method for preparing corn peptide by heat treatment-assisted enzyme method ) 是由 刘峰 袁学宾 姚康 涂强 王娜娜 梁淑淑 刘亚琦 于 2020-05-19 设计创作，主要内容包括：一种热处理辅助酶法制备玉米肽的制备方法,包括如下步骤：步骤一：将玉米蛋白粉粗品粉碎细化,将粉碎后的玉米蛋白粉加入蒸馏水充分均匀混合得玉米蛋白粉溶液；步骤二：将步骤一所得的玉米蛋白粉溶液加热至90-120℃,保温至20-40min；步骤三：步骤二加热完成后降温至50-60℃,加入蛋白酶,在搅拌条件下酶解反应4-8h；步骤四：将步骤三中经过酶解反应后的的混合液在125-150℃的温度下干燥2-3h,既得玉米肽产品。本发明所得的玉米肽蒸馏水分含量≤5％,粗蛋白含量≥55％,分子量1000Da以下的小肽含量≥90％。在提高了玉米肽收率的同时还能确保玉米肽的品质。(A preparation method for preparing corn peptide by heat treatment assisted enzyme method comprises the following steps: the method comprises the following steps: pulverizing and refining the crude corn protein powder, adding distilled water into the pulverized corn protein powder, and fully and uniformly mixing to obtain a corn protein powder solution; step two: heating the corn protein powder solution obtained in the step one to 90-120 ℃, and keeping the temperature for 20-40 min; step three: after the heating in the second step is finished, cooling to 50-60 ℃, adding protease, and carrying out enzymolysis reaction for 4-8h under the stirring condition; step four: and (3) drying the mixed solution subjected to the enzymolysis reaction in the third step at the temperature of 125-150 ℃ for 2-3h to obtain the corn peptide product. The corn peptide obtained by the invention has the distilled water content of less than or equal to 5 percent, the crude protein content of more than or equal to 55 percent and the small peptide content of less than 1000Da of molecular weight of more than or equal to 90 percent. The corn peptide yield is improved, and the quality of the corn peptide can be ensured.)

1. A preparation method for preparing corn peptide by a heat treatment-assisted enzyme method is characterized by comprising the following steps: the method comprises the following steps:

the method comprises the following steps: pulverizing and refining the crude corn protein powder, adding distilled water into the pulverized corn protein powder, and fully and uniformly mixing to obtain a corn protein powder solution;

step two: heating the corn protein powder solution obtained in the step one to 90-120 ℃, and keeping the temperature for 20-40 min;

step three: after the heating in the second step is finished, cooling to 50-60 ℃, adding protease, and carrying out enzymolysis reaction for 4-8h under the stirring condition;

step four: and (3) drying the mixed solution subjected to the enzymolysis reaction in the third step at the temperature of 125-150 ℃ for 2-3h to obtain the corn peptide product.

2. The method for preparing corn peptide by heat treatment assisted enzyme method according to claim 1, wherein the method comprises the following steps: and in the step one, the crude corn protein powder is crushed and refined and then passes through a 40-mesh sieve for later use, and the particles with the particle size larger than 40-mesh sieve are continuously crushed until all the particles pass through the 40-mesh sieve.

3. The method for preparing corn peptide by heat treatment assisted enzyme method according to claim 1, wherein the method comprises the following steps: the corn protein powder solid content of the corn protein powder solution obtained in the step one is 8-20%.

4. The method for preparing corn peptide by heat treatment assisted enzyme method according to claim 1, wherein the method comprises the following steps: the protease in the third step is a mixture obtained by mixing any one or more than two of alkaline protease, neutral protease and flavourzyme in any proportion.

5. The method for preparing corn peptide by heat treatment assisted enzyme method according to claim 1, wherein the method comprises the following steps: the addition amount of the protease in the third step is 0.3-3.0% of the mass of the corn protein powder.

Technical Field

The invention belongs to the field of preparation of corn peptides, and particularly relates to a preparation method for preparing corn peptides by using a heat treatment-assisted enzyme method and a preparation method thereof.

Background

The corn protein powder is commonly called as corn gluten meal, the protein content of the corn gluten meal is about 70 percent, and the corn peptide with small molecular weight can be obtained after the corn gluten meal is distilled and hydrolyzed by protease. The corn peptide has the functions of providing essential amino acid and nutrition for organisms, improving immunity, reducing blood pressure, improving digestion and absorption rate of the organisms and the like, and is easy to absorb. These functions and advantages make maize peptides a research hotspot.

Because the yield of the current method for producing the corn peptide is low and the content of the corn peptide protein is low, the corn protein powder is pretreated to improve the enzymolysis efficiency and the yield of the corn peptide. At present, common pretreatment modes include ultrasonic, microwave, high pressure and the like, but practice, use and research show that the conventional pretreatment modes have various problems, the obtained products have different quality, the obtained corn peptide has larger molecular weight, and a method which can improve the yield of the corn peptide and ensure the quality of the corn peptide products is lacked at present.

Disclosure of Invention

The invention provides a preparation method for preparing corn peptide by a heat treatment-assisted enzyme method, which is used for overcoming the defects in the prior art.

The invention is realized by the following technical scheme:

a preparation method for preparing corn peptide by heat treatment assisted enzyme method comprises the following steps:

the method comprises the following steps: pulverizing and refining the crude corn protein powder, adding distilled water into the pulverized corn protein powder, and fully and uniformly mixing to obtain a corn protein powder solution;

step two: heating the corn protein powder solution obtained in the step one to 90-120 ℃, and keeping the temperature for 20-40 min;

step three: after the heating in the second step is finished, cooling to 50-60 ℃, adding protease, and carrying out enzymolysis reaction for 4-8h under the stirring condition;

step four: and (3) drying the mixed solution subjected to the enzymolysis reaction in the third step at the temperature of 125-150 ℃ for 2-3h to obtain the corn peptide product.

The preparation method for preparing the corn peptide by the heat treatment-assisted enzyme method comprises the following steps of crushing and refining the crude corn protein powder, sieving the crushed crude corn protein powder with a 40-mesh sieve for later use, and continuously crushing the particles with the particle size larger than the 40-mesh sieve until all the particles pass through the 40-mesh sieve.

In the preparation method for preparing the corn peptide by the heat treatment-assisted enzyme method, the corn protein powder solid content of the corn protein powder solution obtained in the first step is 8-20%.

In the method for preparing corn peptide by using the heat treatment-assisted enzyme method, the protease in the third step is a mixture obtained by mixing any one or more of alkaline protease, neutral protease and flavourzyme in any proportion.

In the preparation method for preparing the corn peptide by the heat treatment-assisted enzyme method, the addition amount of the protease in the third step is 0.3-3.0% of the mass of the corn protein powder.

The invention has the advantages that: the corn peptide obtained by the invention has the distilled water content of less than or equal to 5 percent, the crude protein content of more than or equal to 55 percent and the small peptide content of less than 1000Da of molecular weight of more than or equal to 90 percent. The corn peptide yield is improved, and the quality of the corn peptide can be ensured; the corn protein powder is treated by heat treatment, and the heat treatment is green and environment-friendly, has no pollution and damage to the environment, and has the advantages of simple operation, economy, energy conservation and the like; meanwhile, the heat-treated zein can denature proteins through high temperature, and the secondary structure of the proteins, particularly disulfide bonds and hydrophobic and distilled water bonds, is changed, so that the combination of protease and the bottom is increased to a certain extent, and the effects of improving the yield of the zein and ensuring the quality are achieved.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.