阅读说明：本技术 一种用于制备头孢菌素c的发酵培养基 (Fermentation medium for preparing cephalosporin C ) 是由 周如国 宋更申 高彬洁 顾洹 于 2019-12-09 设计创作，主要内容包括：本发明涉及一种用于制备头孢菌素C的发酵培养基,包括如下组成：以去生活饮用水为介质,花生饼粉1.62g/L、秸秆糖0.082g/L、葡萄糖0.083g/L、玉米浆5.05g/L、糊精3.25g/L、豆油5.15g/L、硫酸铵0.645g/L、磷酸二氢钾0.235g/L、七水硫酸镁0.185g/L、轻质碳酸钙0.385g/L、五水硫酸铜0.0021g/L、七水硫酸锌0.0020g/L、一水硫酸锰0.0021g/L、七水硫酸亚铁0.0061g/L、硫酸钙0.685g/L、DL-蛋氨酸0.465g/L、玉米粉1.135g/L、谷朊粉1.915g/L、消沫剂0.035g/L。本发明的发酵培养基中通过加入秸秆糖,大大降低了葡萄糖的使用量,在保证发酵质量的前提下,降低了生产成本(秸秆糖的价格远远低于葡萄糖的价格)。(The invention relates to a fermentation medium for preparing cephalosporin C, which comprises the following components: 1.62g/L of peanut cake powder, 0.082g/L of straw sugar, 0.083g/L of glucose, 5.05g/L of corn steep liquor, 3.25g/L of dextrin, 5.15g/L of soybean oil, 0.645g/L of ammonium sulfate, 0.235g/L of monopotassium phosphate, 0.185g/L of magnesium sulfate heptahydrate, 0.385g/L of light calcium carbonate, 0.0021g/L of copper sulfate pentahydrate, 0.0020g/L of zinc sulfate heptahydrate, 0.0021g/L of manganese sulfate monohydrate, 0.0061g/L of ferrous sulfate heptahydrate, 0.685g/L, DL-methionine, 1.135g/L of corn flour, 1.915g/L of gluten powder and 0.035g/L of defoaming agent. The fermentation medium of the invention is added with the straw sugar, thereby greatly reducing the use amount of the glucose and reducing the production cost (the price of the straw sugar is far lower than that of the glucose) on the premise of ensuring the fermentation quality.)

1. A fermentation medium for the preparation of cephalosporin C, characterized in that: comprises the following components: 1.5-1.7g/L of peanut cake powder, 0.08-0.09g/L of straw sugar, 0.08-0.09g/L of glucose, 4.9-5.1g/L of corn steep liquor, 3.1-3.3g/L of dextrin, 5.0-5.2g/L of soybean oil, 0.63-0.65g/L of ammonium sulfate, 0.22-0.24g/L of potassium dihydrogen phosphate, 0.18-0.20g/L of magnesium sulfate heptahydrate, 0.37-0.39g/L of light calcium carbonate, 0.0019-0.0021g/L of copper sulfate pentahydrate, 0.0019-0.0021g/L of zinc sulfate heptahydrate, 0.0019-0.0021g/L of manganese sulfate monohydrate, 0.0059-0.0061g/L of ferrous sulfate heptahydrate, 0.67-0.69g/L of corn flour, 0. L, DL-0.45-0.47 g/L of methionine, and 14.47-1 g/L of methionine, Gluten powder 1.91-1.93g/L, and defoaming agent 0.02-0.04 g/L.

2. A fermentation medium for the preparation of cephalosporin C according to claim 1, characterized in that: comprises the following components: 1.62g/L of peanut cake powder, 0.082g/L of straw sugar, 0.083g/L of glucose, 5.05g/L of corn steep liquor, 3.25g/L of dextrin, 5.15g/L of soybean oil, 0.645g/L of ammonium sulfate, 0.235g/L of monopotassium phosphate, 0.185g/L of magnesium sulfate heptahydrate, 0.385g/L of light calcium carbonate, 0.0021g/L of copper sulfate pentahydrate, 0.0020g/L of zinc sulfate heptahydrate, 0.0021g/L of manganese sulfate monohydrate, 0.0061g/L of ferrous sulfate heptahydrate, 0.685g/L, DL-methionine, 1.135g/L of corn flour, 1.915g/L of gluten powder and 0.035g/L of defoaming agent.

3. A fermentation medium for the preparation of cephalosporin C according to claim 1, characterized in that: comprises the following components: 1.65g/L of peanut cake powder, 0.085g/L of straw sugar, 0.085g/L of glucose, 5g/L of corn steep liquor, 3.2g/L of dextrin, 5.1g/L of soybean oil, 0.64g/L of ammonium sulfate, 0.23g/L of monopotassium phosphate, 0.19g/L of magnesium sulfate heptahydrate, 0.38g/L of light calcium carbonate, 0.0020g/L of copper sulfate pentahydrate, 0.0020g/L of zinc sulfate heptahydrate, 0.0020g/L of manganese sulfate monohydrate, 0.0060g/L of ferrous sulfate heptahydrate, 0.68g/L, DL-methionine, 1.13g/L of corn flour, 1.92g/L of gluten powder and 0.03g/L of defoaming agent by taking life-free drinking water as a medium.

4. A process for the preparation of a fermentation medium for the preparation of cephalosporin C as claimed in any of claims 1 to 3, characterized in that: the method comprises the following specific steps:

the raw materials are put into a fermentation tank according to the weight ratio, after the pH value is adjusted to 6.5-7.5, the pressure is maintained for 25-35 minutes under the conditions of 0.10-0.13MPa and 120-125 ℃, then the temperature is reduced to 26-30 ℃, and the pH value is adjusted to 7.0-7.3 to wait for inoculation.

Technical Field

The invention belongs to the technical field of biological pharmacy, and particularly relates to a fermentation medium for preparing cephalosporin C.

Background

Cephalosporin C (CPC) is β -lactam antibiotic, has the characteristics of wide antibacterial spectrum, high activity, good curative effect and low toxicity, is an antibiotic variety with the widest application at present, and a fermentation production strain of the cephalosporin C is Cephalosporium acremonium (CPC) which is a secondary metabolite of Cephalosporium acremonium.

The cephalosporin C is used as a semi-synthetic precursor of the cephalosporin antibiotics, the production level of the cephalosporin C determines the market supply amount of the cephalosporin antibiotics, and the cephalosporin C has important social and economic significance for the deep research and process optimization of a fermentation method for preparing the cephalosporin C. It is well known that fermentation medium is an important factor affecting fermentation, and therefore, it is important how to provide a suitable fermentation medium for the preparation of cephalosporin C.

Disclosure of Invention

The invention aims to: a fermentation medium for the preparation of cephalosporin C is provided.

In order to achieve the above purpose, the invention provides the following technical scheme:

a fermentation medium for the preparation of cephalosporin C comprising the following composition: 1.5-1.7g/L of peanut cake powder, 0.08-0.09g/L of straw sugar, 0.08-0.09g/L of glucose, 4.9-5.1g/L of corn steep liquor, 3.1-3.3g/L of dextrin, 5.0-5.2g/L of soybean oil, 0.63-0.65g/L of ammonium sulfate, 0.22-0.24g/L of potassium dihydrogen phosphate, 0.18-0.20g/L of magnesium sulfate heptahydrate, 0.37-0.39g/L of light calcium carbonate, 0.0019-0.0021g/L of copper sulfate pentahydrate, 0.0019-0.0021g/L of zinc sulfate heptahydrate, 0.0019-0.0021g/L of manganese sulfate monohydrate, 0.0059-0.0061g/L of ferrous sulfate heptahydrate, 0.67-0.69g/L of corn flour, 0. L, DL-0.45-0.47 g/L of methionine, and 14.47-1 g/L of methionine, Gluten powder 1.91-1.93g/L, and defoaming agent 0.02-0.04 g/L.

Preferably, a fermentation medium for the preparation of cephalosporin C, comprising the following composition: 1.62g/L of peanut cake powder, 0.082g/L of straw sugar, 0.083g/L of glucose, 5.05g/L of corn steep liquor, 3.25g/L of dextrin, 5.15g/L of soybean oil, 0.645g/L of ammonium sulfate, 0.235g/L of monopotassium phosphate, 0.185g/L of magnesium sulfate heptahydrate, 0.385g/L of light calcium carbonate, 0.0021g/L of copper sulfate pentahydrate, 0.0020g/L of zinc sulfate heptahydrate, 0.0021g/L of manganese sulfate monohydrate, 0.0061g/L of ferrous sulfate heptahydrate, 0.685g/L, DL-methionine, 1.135g/L of corn flour, 1.915g/L of gluten powder and 0.035g/L of defoaming agent.

Preferably, a fermentation medium for the preparation of cephalosporin C, comprising the following composition: 1.65g/L of peanut cake powder, 0.085g/L of straw sugar, 0.085g/L of glucose, 5g/L of corn steep liquor, 3.2g/L of dextrin, 5.1g/L of soybean oil, 0.64g/L of ammonium sulfate, 0.23g/L of monopotassium phosphate, 0.19g/L of magnesium sulfate heptahydrate, 0.38g/L of light calcium carbonate, 0.0020g/L of copper sulfate pentahydrate, 0.0020g/L of zinc sulfate heptahydrate, 0.0020g/L of manganese sulfate monohydrate, 0.0060g/L of ferrous sulfate heptahydrate, 0.68g/L, DL-methionine, 1.13g/L of corn flour, 1.92g/L of gluten powder and 0.03g/L of defoaming agent by taking life-free drinking water as a medium.

A preparation method of a fermentation medium for preparing cephalosporin C comprises the following specific steps:

the raw materials are put into a fermentation tank according to the weight ratio, after the pH value is adjusted to 6.5-7.5, the pressure is maintained for 25-35 minutes under the conditions of 0.10-0.13MPa and 120-125 ℃, then the temperature is reduced to 26-30 ℃, and the pH value is adjusted to 7.0-7.3 to wait for inoculation.

The invention has the beneficial effects that:

the fermentation medium of the invention is added with the straw sugar, thereby greatly reducing the use amount of the glucose and reducing the production cost (the price of the straw sugar is far lower than that of the glucose) on the premise of ensuring the fermentation quality.

DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION