阅读说明：本技术 在至少两个波长范围之间进行辨别的高分辨率扫描显微术 (High resolution scanning microscopy for discriminating between at least two wavelength ranges ) 是由 I.克莱普 R.内茨 于 2018-06-21 设计创作，主要内容包括：出于高分辨率扫描显微术的目的,样品(2)由照明辐射(5)激发以发射荧光辐射,使得将照明辐射聚焦在样品(2)中或样品(2)上的点处,以形成衍射受限的照明光斑(14)。以衍射受限的方式将点成像在空间分辨二维检测器(19)上的衍射图像(23)中,其中二维检测器(19)具有对衍射图像(23)的结构进行分辨的空间分辨率。以小于照明光斑(14)的一半直径的增量,通过不同的扫描位置来扫描样品(2)。从二维检测器(19)的数据以及从向这些数据分配的扫描位置生成样品(2)的图像,该图像的分辨率增加以超过成像的分辨率极限。出于在样品(2)的荧光辐射中在至少两个预先确定的波长范围之间进行辨别的目的,对应于至少两个预先确定的波长范围的一定数目的衍射结构(30-37)生成在二维检测器(19)上,所述衍射结构区别于彼此但是具有共同的对称中心(40)。当生成样品(2)的图像时评估衍射结构(30-37)。(For the purpose of high resolution scanning microscopy, the sample (2) is excited by the illumination radiation (5) to emit fluorescence radiation such that the illumination radiation is focused at a point in or on the sample (2) to form a diffraction limited illumination spot (14). The spots are imaged in a diffraction-limited manner in a diffraction image (23) on a spatially resolved two-dimensional detector (19), wherein the two-dimensional detector (19) has a spatial resolution which resolves the structure of the diffraction image (23). The sample (2) is scanned by different scanning positions in increments smaller than half the diameter of the illumination spot (14). An image of the sample (2) is generated from the data of the two-dimensional detector (19) and from the scanning positions assigned to these data, the resolution of the image being increased beyond the resolution limit of the imaging. For the purpose of discriminating between at least two predetermined wavelength ranges in the fluorescence radiation of the sample (2), a number of diffractive structures (30-37) corresponding to the at least two predetermined wavelength ranges are generated on a two-dimensional detector (19), said diffractive structures being distinct from each other but having a common center of symmetry (40). The diffractive structures (30-37) are evaluated when generating an image of the sample (2).)