阅读说明：本技术 一种植物生根壮苗培养基 (Plant rooting and seedling strengthening culture medium ) 是由 高洁 谭志毅 王平 贾俊高 高行英 佘波 张晓玲 王煜慧 于 2019-12-19 设计创作，主要内容包括：本发明公开了一种植物生根壮苗培养基,该培养基的配方为：MS基本培养基+0.8～1.5mg/L 6-BA +0.75～0.95mg/L鼠李糖脂+20～30mg/L密罗木提取物+2～4mg/L极细链格孢激活蛋白+4～6mg/L糖醇螯合复合肥+0.05～0.1g/L聚谷氨酸+2～4g/L氨基寡糖素+6～10g/L琼脂粉+20～30g/L蔗糖。本发明的培养基既能为植物组织的生长提供需要的营养成分,又能提高植物组织对吸收养分的能力,从而可以有效缩短诱导生根时间,根系发育良好,30天生根率达100%,平均每株生根7～8根,植株健壮；移栽成活率高,可达到95%。(The invention discloses a plant rooting and seedling strengthening culture medium, which comprises the following components in part by weight: MS basic culture medium, 0.8-1.5 mg/L6-BA, 0.75-0.95 mg/L rhamnolipid, 20-30 mg/L millettia extract, 2-4 mg/L alternaria tenuissima activator protein, 4-6 mg/L sugar alcohol chelate compound fertilizer, 0.05-0.1 g/L polyglutamic acid, 2-4 g/L amino-oligosaccharin, 6-10 g/L agar powder and 20-30 g/L sucrose. The culture medium can provide required nutrient components for the growth of plant tissues and improve the capability of the plant tissues to absorb nutrients, so that the induced rooting time can be effectively shortened, the root system is well developed, the rooting rate reaches 100% in 30 days, each plant takes roots 7-8 on average, and the plant is robust; the transplanting survival rate is high and can reach 95 percent.)

1. A plant rooting and seedling strengthening culture medium is characterized in that the formula of the culture medium is as follows:

MS basic culture medium, 0.8-1.5 mg/L6-BA, 0.75-0.95 mg/L rhamnolipid, 20-30 mg/L millettia extract, 2-4 mg/L alternaria tenuissima activator protein, 4-6 mg/L sugar alcohol chelate compound fertilizer, 0.05-0.1 g/L polyglutamic acid, 2-4 g/L amino-oligosaccharin, 6-10 g/L agar powder and 20-30 g/L sucrose.

2. The plant rooting and seedling strengthening culture medium of claim 1, wherein the formula of the culture medium is as follows:

MS minimal medium +0.8 mg/L6-BA +0.75 mg/L rhamnolipid +20mg/L millettia extract +2mg/L alternaria tenuissima activator protein +4mg/L sugar alcohol chelate compound fertilizer +0.05g/L polyglutamic acid +2g/L amino-oligosaccharin +6g/L agar powder +20g/L sucrose.

3. The plant rooting and seedling strengthening culture medium of claim 1, wherein the formula of the culture medium is as follows:

MS minimal medium +1.5 mg/L6-BA +0.95mg/L rhamnolipid +30mg/L millettia extract +4mg/L alternaria tenuissima activator protein +6mg/L sugar alcohol chelate compound fertilizer +0.1g/L polyglutamic acid +4g/L amino-oligosaccharin +10g/L agar powder +30g/L sucrose.

4. The plant rooting and seedling strengthening culture medium of claim 1, wherein the formula of the culture medium is as follows:

MS minimal medium +1.65 mg/L6-BA +1.7mg/L rhamnolipid +25mg/L millettia extract +3mg/L alternaria tenuissima activator protein +5mg/L sugar alcohol chelate compound fertilizer +0.075g/L polyglutamic acid +3g/L amino-oligosaccharin +8g/L agar powder +25g/L sucrose.

5. The plant rooting and seedling strengthening culture medium of claim 1, wherein the formula of the culture medium is as follows:

MS minimal medium +0.8 mg/L6-BA +0.7 mg/L rhamnolipid +22mg/L millettia extract +2mg/L alternaria tenuissima activator protein +4mg/L sugar alcohol chelate compound fertilizer +0.05g/L polyglutamic acid +2g/L amino-oligosaccharin +6g/L agar powder +20g/L sucrose.

6. The plant rooting and seedling strengthening culture medium of claim 1, wherein the formula of the culture medium is as follows:

MS minimal medium +0.8 mg/L6-BA +0.8 mg/L rhamnolipid +28mg/L millettia extract +3mg/L alternaria tenuissima activator protein +4mg/L sugar alcohol chelate compound fertilizer +0.08g/L polyglutamic acid +2g/L amino-oligosaccharin +6g/L agar powder +20g/L sucrose.

Technical Field

The invention relates to the field of culture media, in particular to a plant rooting and seedling strengthening culture medium.

Background

Plant tissue culture is a biotechnology that uses a part of a plant body to perform asexual propagation to produce a large number of homologous maternal genetic seedlings. The tissue culture technology has the advantages of controlling the growth and differentiation of cells by utilizing various artificial culture conditions, and the like, effectively promotes the cross development of plant physiology, pathology, pharmacy, genetics, breeding, biochemistry and other subjects, is widely applied to various industries such as agriculture, forestry, gardening, industry, pharmaceutical industry and the like, and generates great economic benefit and social benefit.

Dedifferentiation and differentiation are important links of plant tissue culture, and play a decisive influence on the tissue culture efficiency. In addition, the test-tube plantlets obtained by tissue culture and gene transformation are often weak in growth vigor, undeveloped in root system and low in survival rate after transplantation, and the culture efficiency and further application effect of the test-tube plantlets are seriously influenced. Therefore, it is very important to establish an efficient plant rooting and seedling strengthening system.

Disclosure of Invention

The invention aims to provide a plant rooting and seedling strengthening culture medium, which effectively shortens the time of inducing rooting, and has the advantages of good root system development, robust plants and high transplanting survival rate.

In order to achieve the purpose, the invention adopts the technical scheme that:

a plant rooting and seedling strengthening culture medium comprises the following components:

MS basic culture medium, 0.8-1.5 mg/L6-BA, 0.75-0.95 mg/L rhamnolipid, 20-30 mg/L millettia extract, 2-4 mg/L alternaria tenuissima activator protein, 4-6 mg/L sugar alcohol chelate compound fertilizer, 0.05-0.1 g/L polyglutamic acid, 2-4 g/L amino-oligosaccharin, 6-10 g/L agar powder and 20-30 g/L sucrose.

Preferably, the formula of the culture medium is as follows:

MS minimal medium +0.8 mg/L6-BA +0.75 mg/L rhamnolipid +20mg/L millettia extract +2mg/L alternaria tenuissima activator protein +4mg/L sugar alcohol chelate compound fertilizer +0.05g/L polyglutamic acid +2g/L amino-oligosaccharin +6g/L agar powder +20g/L sucrose.

Preferably, the formula of the culture medium is as follows:

MS minimal medium +1.5 mg/L6-BA +0.95mg/L rhamnolipid +30mg/L millettia extract +4mg/L alternaria tenuissima activator protein +6mg/L sugar alcohol chelate compound fertilizer +0.1g/L polyglutamic acid +4g/L amino-oligosaccharin +10g/L agar powder +30g/L sucrose.

Preferably, the formula of the culture medium is as follows:

MS minimal medium +1.65 mg/L6-BA +1.7mg/L rhamnolipid +25mg/L millettia extract +3mg/L alternaria tenuissima activator protein +5mg/L sugar alcohol chelate compound fertilizer +0.075g/L polyglutamic acid +3g/L amino-oligosaccharin +8g/L agar powder +25g/L sucrose.

Preferably, the formula of the culture medium is as follows:

MS minimal medium +0.8 mg/L6-BA +0.7 mg/L rhamnolipid +22mg/L millettia extract +2mg/L alternaria tenuissima activator protein +4mg/L sugar alcohol chelate compound fertilizer +0.05g/L polyglutamic acid +2g/L amino-oligosaccharin +6g/L agar powder +20g/L sucrose.

Preferably, the formula of the culture medium is as follows:

MS minimal medium +0.8 mg/L6-BA +0.8 mg/L rhamnolipid +28mg/L millettia extract +3mg/L alternaria tenuissima activator protein +4mg/L sugar alcohol chelate compound fertilizer +0.08g/L polyglutamic acid +2g/L amino-oligosaccharin +6g/L agar powder +20g/L sucrose.

The invention has the following beneficial effects:

the culture medium can provide required nutrient components for the growth of plant tissues and improve the capability of the plant tissues to absorb nutrients, so that the induced rooting time can be effectively shortened, the root system is well developed, the rooting rate reaches 100% in 30 days, each plant takes roots 7-8 on average, and the plant is robust; the transplanting survival rate is high and can reach 95 percent.

Detailed Description

In order that the objects and advantages of the invention will be more clearly understood, the invention is further described in detail below with reference to examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.