阅读说明：本技术 一种红雨伞的无性繁殖的方法 (Asexual propagation method of umbrellas roses ) 是由 不公告发明人 于 2019-12-06 设计创作，主要内容包括：本发明涉及一种红雨伞的无性繁殖方法,包括：(1)外植体预处理；(2)将预处理后的外植体切割成若干个小外植体,每个小外植体带有1-3个茎节,将小外植体接种到诱导培养基上培养至丛生芽形成；(3)分离诱导培养基中的丛生芽,使丛生芽长度控制在2-3cm；将分离后的丛生芽接种至增殖培养基中培养,待新生芽生长至2-3cm,分离新生芽并将其插于增殖培养基中重复继代培养3-4次,获得大量扩繁后的丛生芽；(4)将扩繁后的丛生芽中长度3-5cm的丛生芽从基部自芽块上拆分下来,扦插并定植。该方法不受季节、温度、地域影响就能获得大量的新植株,从而快速建立红雨伞无菌繁殖体系,为保持红雨伞优良的种性提供技术支持,为广大的爱好者提供源源不断地无菌种苗。(The invention relates to a vegetative propagation method of umbrellas rosepellis, which comprises the following steps: (1) pretreating explants; (2) cutting the pretreated explant into a plurality of small explants, wherein each small explant is provided with 1-3 stem nodes, and inoculating the small explants onto an induction culture medium to be cultured until cluster buds are formed; (3) separating cluster buds in the induction culture medium, and controlling the length of the cluster buds to be 2-3 cm; inoculating the separated cluster buds into a multiplication culture medium for culture, separating the new buds when the new buds grow to 2-3cm, and inserting the new buds into the multiplication culture medium for repeated subculture for 3-4 times to obtain a large number of expanded cluster buds; (4) splitting the cluster buds with the length of 3-5cm from the base self-bud blocks after propagation, cutting and planting. The method can obtain a large amount of new plants without being influenced by seasons, temperature and regions, thereby quickly establishing a red umbrella sterile propagation system, providing technical support for keeping excellent seed properties of the red umbrella, and providing continuous sterile seedlings for vast enthusiasts.)

1. A vegetative propagation method of a red umbrella is characterized by comprising the following steps:

(1) pretreatment: selecting a robust and non-defective red umbrella plant, and performing sterilization treatment and cleaning to obtain a sterilized explant;

(2) and (3) induction culture: cutting the sterilized explant into a plurality of small explants in a sterile culture dish, wherein each small explant is provided with 1-3 stem nodes, and inoculating the small explants onto an induction culture medium for induction culture for 7-15 days until cluster buds are formed;

(3) subculturing: separating cluster buds in the induction culture medium, and controlling the length of the cluster buds to be 2-3 cm; inoculating the separated cluster buds into a multiplication culture medium for subculture, separating the new buds when the new buds grow to 2-3cm, obliquely inserting or horizontally placing the new buds into the multiplication culture medium, and repeating the subculture for 3-4 times to obtain a large number of propagated cluster buds;

(4) rooting and field planting: and (4) separating the cluster buds propagated in the step (3), cutting the cluster buds into an aquarium for rooting and planting.

2. The vegetative propagation method of umbrellas as claimed in claim 1, wherein the explant sterilization treatment and washing in step (1) are specifically performed by the following steps: rapidly washing with tap water for 20 minutes, removing needle-shaped leaves of the red umbrella, and protecting the epidermis of the stem; then the water flow is adjusted to be just a line and the washing is continued for 40 minutes; after washing, the explants are placed in hydrogen peroxide for disinfection for 15-20 minutes and then rinsed 3-5 times with sterile water.

3. The method for the vegetative propagation of umbrellas as set forth in claim 1, wherein the induction medium is: MS culture medium +1-2 mg/L6-BA +6-8g/L agar +30g/L sucrose +4-8g/L active carbon +1ml/L plant tissue antibacterial agent.

4. The method for the vegetative propagation of umbrellas as set forth in claim 1, wherein the propagation medium is: MS culture medium +1-5 mg/L6-BA +0.5-1mg/L indoleacetic acid +6-8g/L agar +30g/L sucrose.

5. The method for the vegetative propagation of umbrellas as set forth in claim 1, wherein the inducing culture conditions are: the illumination period is 14h/d, the temperature is 25 ℃, and the illumination is 3000-.

6. The method for the vegetative propagation of umbrellas as set forth in claim 1, wherein the subculture conditions are: the illumination period is 14h/d, the temperature is 25 ℃, and the illumination is 3000-.

7. The method for vegetative propagation of umbrellas as set forth in claim 1, wherein the method for separating the cluster buds after propagation in step (4) comprises: splitting the cluster buds with the length of 3-5cm from the base part of the propagated cluster buds, cleaning and transplanting.

Technical Field

The invention relates to the technical field of plant tissue culture, in particular to a vegetative propagation method of umbrellas rosepella.

Background

Umbrellas, dicotyledons, Ericaceae, emergent aquatic weeds. Aquatic weeds are different from aquatic weeds. The aquatic weeds have needles shaped alternate leaves with emerald green leaves and sawteeth at the edges of the leaves. Many different variations occur when cultivated in water. Including leaf types that can be converted from lanceolate to ovoid, irregular lobes, to feathered lobes, and the like. The color can be changed from green to orange-red to deep red. The shape of the plant is varied depending on the cultivation conditions. Favour strong light and lower water temperature, and grow slowly in water. The red umbrella is rare and expensive, so the red umbrella is often regarded as the classic in the red grass by the float grass, and the red umbrella can change different colors along with different cultivation conditions, so the red umbrella is more attractive. The umbrellas belong to stem type aquatic weeds, and belong to the blank in the field of domestic umbrellas tissue culture.

Disclosure of Invention

Aiming at the technical problems in the prior art, the invention provides an asexual propagation method of a red umbrella. The method is easy to implement, convenient to operate and high in yield, and solves the problems that the aquatic animals are difficult to breed and the breeding speed is low in the aquarium.

In order to realize the purpose, the invention is realized by the following technical scheme:

a vegetative propagation method of umbrellas roseumbrellas comprises the following steps:

(1) pretreatment: selecting a robust and non-defective red umbrella plant, and performing sterilization treatment and cleaning to obtain a sterilized explant;

(2) and (3) induction culture: cutting the sterilized explant into a plurality of small explants in a sterile culture dish, wherein each small explant is provided with 1-3 stem nodes, and inoculating the small explants onto an induction culture medium for induction culture for 7-15 days until cluster buds are formed;

(3) subculturing: separating cluster buds in the induction culture medium, and controlling the length of the cluster buds to be 2-3 cm; inoculating the separated cluster buds into a multiplication culture medium for subculture, separating the new buds when the new buds grow to 2-3cm, obliquely inserting or horizontally placing the new buds into the multiplication culture medium, and repeating the subculture for 3-4 times to obtain a large number of propagated cluster buds;

(4) rooting and field planting: and (4) separating the cluster buds propagated in the step (3), cutting the cluster buds into an aquarium for rooting and planting.

Further, the specific method for sterilizing and cleaning the explant in the step (1) comprises the following steps: rapidly washing with tap water for 20 minutes, removing needle-shaped leaves of the red umbrella, and protecting the epidermis of the stem; then the water flow is adjusted to be just a line and the washing is continued for 40 minutes; after washing, the explants are placed in hydrogen peroxide for disinfection for 15-20 minutes and then rinsed 3-5 times with sterile water.

Further, the induction medium is: MS culture medium +1-2 mg/L6-BA +6-8g/L agar +30g/L sucrose +4-8g/L active carbon +1ml/L plant tissue antibacterial agent.

Further, the proliferation medium is: MS culture medium +1-5 mg/L6-BA +0.5-1mg/L indoleacetic acid +6-8g/L agar +30g/L sucrose.

Further, the induction culture conditions are as follows: the illumination period is 14h/d, the temperature is 25 ℃, and the illumination is 3000-.

Further, the subculture conditions are: the illumination period is 14h/d, the temperature is 25 ℃, and the illumination is 3000-.

Further, the method for separating the cluster buds after propagation in the step (4) comprises the following steps: splitting the cluster buds with the length of 3-5cm from the base part of the propagated cluster buds, cleaning and transplanting.

Therefore, compared with the prior art, the invention has the following beneficial technical effects:

(1) the vegetative propagation method of the red umbrella provided by the invention is characterized in that under the aseptic condition, a proper culture environment is adopted, fixed illumination is given, the growth of the buds of the red umbrella is directly induced, the influence of seasons, temperature and regions can be avoided, a large number of new plants can be obtained, thus a red umbrella aseptic propagation system is quickly established, technical support is provided for keeping the excellent seed nature of the red umbrella, and continuous aseptic seedlings are provided for vast enthusiasts. Experiments prove that through one-month culture, the tissue culture method can enable one explant to quickly obtain 20 +/-5 new progeny plants.

(2) The tissue culture formulas adopted in the tissue culture method are cooperated, the tissue propagation period is short, the propagation rate is high, the rooting rate of the red umbrella seedlings aseptically cultured by the tissue culture method in natural water is up to 90%, the plant survival rate is up to more than 95%, the effective and rapid asexual propagation of the red umbrella is realized, and the method plays an important role in promoting the production of ornamental aquatic weeds, cultivating new species and accelerating the rapid development of aquatic ornamental aquatic weed industry.

(3) The tissue culture method of the aseptic seedlings of the umbrellas seedlings is simple and easy to implement, convenient to operate and high in yield, and solves the problems that the propagation is difficult and the propagation speed is slow in an aquarium.

Detailed Description

The following examples are presented to illustrate certain embodiments of the invention in particular and should not be construed as limiting the scope of the invention. The present disclosure may be modified from materials, methods, and reaction conditions at the same time, and all such modifications are intended to be within the spirit and scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art. The reagents and biomaterials, if not specifically indicated, are commercially available.