阅读说明：本技术 一种用于显微镜上拍摄大面积样品的设备及其使用方法 (Equipment for shooting large-area sample on microscope and using method thereof ) 是由 汪涛 胡文平 张小涛 任晓辰 耿博文 张静 席悦 牟麒 于 2019-10-31 设计创作，主要内容包括：本发明公开了一种用于显微镜上拍摄大面积样品的设备及其使用方法,在显微镜的底座上设置两个正交叠放安装在一起的电动平移台,位于上部的电动平移台的滑块作为样品放置台；显微镜的顶部具有图像采集传感器,用于对样品进行拍照采集其图像；显微镜的图像采集传感器与上位机连接,由上位机控制图像采集传感器进行拍照,图像采集传感器拍照采集的图像传回给上位机进行后续图像的拼接处理；电动平移台与上位机连接,由上位机控制两个电动平移台运动,进而控制样品的拍摄位置。本发明可充分利用光学偏振光显微镜的成像优势,对比数码相机照片,上百倍增加拍照的分辨率,对现有显微镜成像范围带来突破。(The invention discloses a device for shooting a large-area sample on a microscope and a using method thereof.A base of the microscope is provided with two electric translation tables which are orthogonally stacked and installed together, and a slide block of the electric translation table positioned at the upper part is used as a sample placing table; the top of the microscope is provided with an image acquisition sensor which is used for photographing a sample and acquiring an image of the sample; an image acquisition sensor of the microscope is connected with an upper computer, the upper computer controls the image acquisition sensor to take a picture, and an image acquired by the image acquisition sensor by taking the picture is transmitted back to the upper computer to carry out subsequent image splicing processing; the electric translation platforms are connected with an upper computer, and the upper computer controls the two electric translation platforms to move so as to control the shooting position of the sample. The invention can fully utilize the imaging advantages of the optical polarized light microscope, increase the photographing resolution by hundreds of times compared with the photos of the digital camera, and bring breakthrough to the imaging range of the existing microscope.)

1. An apparatus for taking large area samples on a microscope, characterized by: the microscope comprises a microscope and an upper computer, wherein two electric translation stages which are orthogonally stacked and installed together are arranged on a base of the microscope, and a sliding block of the electric translation stage positioned at the upper part is used as a sample placing stage;

the top of the microscope is provided with an image acquisition sensor which is used for photographing a sample and acquiring an image of the sample; an image acquisition sensor of the microscope is connected with an upper computer, the upper computer controls the image acquisition sensor to take a picture, and an image acquired by the image acquisition sensor by taking the picture is transmitted back to the upper computer to carry out subsequent image splicing processing;

the electric translation stages are connected with an upper computer, and the upper computer controls the two electric translation stages to move so as to control the shooting position of the sample.

2. The apparatus of claim 1 for photographing large area samples on a microscope, characterized in that: the microscope is a polarized light microscope.

3. The apparatus of claim 1 for photographing large area samples on a microscope, characterized in that: the image acquisition sensor is a CCD image acquisition sensor.

4. The apparatus of claim 1 for photographing large area samples on a microscope, characterized in that: the electric translation stage adopts a roller bearing electric translation stage driven by a stepping motor.

5. The apparatus of claim 1 for photographing large area samples on a microscope, characterized in that: the upper computer end utilizes LabVIEW software to control the moving step length, the moving direction and the waiting time of the electric translation stage.

6. Use of an apparatus for taking large area samples on a microscope according to claim 1 characterised in that:

the method comprises the following steps: placing a sample to be shot on a slide block of an electric translation table positioned at the upper part;

step two: selecting the objective lens multiple of the microscope, finishing focusing, determining a reasonable imaging range, and setting the photographing moving step length, the moving direction, the waiting time, the photographing interval time and the number of photos of the sample at the upper computer end;

step three: according to the control parameters determined in the second step, the upper computer controls the electric translation stage and the CCD image acquisition sensor to complete image acquisition of the sample;

step four: and finally, carrying out size calibration in the horizontal and vertical directions on the shot photo set, inputting the calibrated offset into software, reading and positioning the shooting coordinates through LabVIEW, and completing splicing of the shot images.

Technical Field

The invention belongs to the technical field of microscopic image acquisition, and particularly relates to equipment for shooting a large-area sample on a microscope.

Background

The image field of the lowest-multiple lens of the current commercial optical polarized light microscope objective is about 1 multiplied by 1cm2, which cannot meet the requirement of directly shooting a sample with a size larger than a centimeter level, and limits the optical characterization of a large-size sample. For large-size samples, particularly crystal samples, although shooting can be completed by using an independent polarizing film together with a commercial digital camera lens, the contrast and resolution of photos cannot meet the requirements of scientific research, and the microstructure of the samples cannot be captured.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide a device for shooting a large-area sample on a microscope.

The invention is realized by the following technical scheme:

the device for shooting a large-area sample on a microscope comprises a microscope and an upper computer, wherein two electric translation stages which are orthogonally stacked and installed together are arranged on a base of the microscope, and a sliding block of the electric translation stage positioned at the upper part is used as a sample placing stage;

the top of the microscope is provided with an image acquisition sensor which is used for photographing a sample and acquiring an image of the sample; an image acquisition sensor of the microscope is connected with an upper computer, the upper computer controls the image acquisition sensor to take a picture, and an image acquired by the image acquisition sensor by taking the picture is transmitted back to the upper computer to carry out subsequent image splicing processing;

the electric translation stages are connected with an upper computer, and the upper computer controls the two electric translation stages to move so as to control the shooting position of the sample.

In the above technical solution, the microscope is a polarized light microscope.

In the above technical solution, the image capturing sensor is a CCD image capturing sensor.

In the above technical solution, the electric translation stage adopts a roller bearing electric translation stage driven by a stepping motor.

In the technical scheme, the upper computer end utilizes LabVIEW software to control the moving step length, the moving direction and the waiting time of the electric translation stage.

The use method of the device for shooting the large-area sample on the microscope is as follows:

the method comprises the following steps: placing a sample to be shot on a slide block of an electric translation table positioned at the upper part;

step two: selecting the objective lens multiple of the microscope, finishing focusing, determining a reasonable imaging range, and setting the photographing moving step length, the moving direction, the waiting time, the photographing interval time and the number of photos of the sample at the upper computer end;

step three: according to the control parameters determined in the second step, the upper computer controls the electric translation stage and the CCD image acquisition sensor to complete image acquisition of the sample;

step four: and finally, carrying out size calibration in the horizontal and vertical directions on the shot photo set, inputting the calibrated offset into software, reading and positioning the shooting coordinates through LabVIEW, and completing splicing of the shot images.

The invention has the advantages and beneficial effects that:

the invention adds a two-axis electric control translation stage on the microscope, controls the translation distance and the photographing through LabVIEW software, and finally performs picture splicing on the photographed pictures of all parts through the software to obtain a large-area sample microscope picture. The method can fully utilize the imaging advantages of the optical polarized light microscope, increase the photographing resolution by hundreds of times compared with the photos of the digital camera, bring breakthrough to the imaging range of the existing microscope, and provide a novel characterization means for scientific research and other fields.

Drawings

Fig. 1 is a schematic structural view of an apparatus for photographing a large-area sample on a microscope according to the present invention.

Fig. 2 is a schematic diagram of a photo stitching method.

Wherein:

1: first motorized translation stage, 2: second motorized translation stage, 3: and a CCD image acquisition sensor.

For a person skilled in the art, other relevant figures can be obtained from the above figures without inventive effort.

Detailed Description

In order to make the technical solution of the present invention better understood, the technical solution of the present invention is further described below with reference to specific examples.