阅读说明：本技术 一种辣木叶有效成分分段提取方法及含有该辣木叶有效成分的饮品和饮品的制备方法 (Segmented extraction method of moringa oleifera leaf effective components, and beverage preparation method containing moringa oleifera leaf effective components ) 是由 杨秀宝 吕国营 于 2019-11-07 设计创作，主要内容包括：本发明涉及辣木提取物及生产方法领域,具体涉及一种辣木叶有效成分分段提取方法及含有该辣木叶有效成分的饮品和饮品的制备方法,包括如下步骤：将原料辣木叶送入热泵干燥机进行热泵干燥；将干燥辣木叶破碎成粉末,进行热风干燥；将粉末和去离子水按混合搅拌并匀质,混合液进行连续双灌提取,水浸提液进行精滤和超滤,喷雾干燥形成水提取物粉末,离心分离所得的固体和乙醇混合,进行超声提取,得到乙醇浸提液；低压蒸发乙醇浸提液中的乙醇,干燥得到乙醇提取物粉末。本发明提供的辣木叶有效成分分段提取方法,能够提取多种有效营养物质,提取率高,对辣木叶中热敏性和光敏性的营养物质破坏少,蛋白质的提取率高。(The invention relates to the field of moringa oleifera extract and a production method thereof, in particular to a segmented extraction method of active ingredients of moringa oleifera leaves and a beverage containing the active ingredients of the moringa oleifera leaves and a preparation method of the beverage, comprising the following steps: feeding the raw material moringa leaves into a heat pump dryer for heat pump drying; crushing the dried moringa leaves into powder, and drying by hot air; mixing the powder and deionized water, stirring, homogenizing, continuously extracting the mixed solution with two tanks, finely filtering and ultrafiltering the water extract, spray drying to obtain water extract powder, centrifuging to obtain solid, mixing with ethanol, and ultrasonically extracting to obtain ethanol extract; evaporating ethanol in the ethanol leaching solution under low pressure, and drying to obtain ethanol extract powder. The method for extracting the active ingredients of the moringa leaves by sections can extract various effective nutrient substances, has high extraction rate, little damage to heat-sensitive and light-sensitive nutrient substances in the moringa leaves and high extraction rate of protein.)

1. A segmented extraction method for active ingredients of moringa leaves is characterized by comprising the following steps:

step 1, feeding the raw material moringa leaves into a heat pump dryer for heat pump drying, wherein the drying temperature is 30-35 ℃, and the drying time is 3-4 hours;

step 2, crushing the dried moringa leaves obtained in the step 1 into powder;

step 3, carrying out hot air drying on the powder obtained in the step 2, wherein the drying temperature is 40-45 ℃, and the drying time is 1-2 hours;

and 4, mixing the powder obtained in the step 3 and deionized water according to the weight ratio of 1: mixing and stirring at the weight ratio of 100-120 and homogenizing;

and 5, regulating the pH value of the homogenized mixed solution to 4-4.5, and carrying out continuous double-irrigation extraction, wherein in the first extraction tank, the ratio of cellulase to the mixed solution 1: adding cellulase in a weight ratio of 100-: adding 100-110 weight percent of composite protease, centrifuging the mixed solution output by the second extraction tank to separate the solid and the liquid to obtain water extract;

step 6, carrying out fine filtration and ultrafiltration on the water extract, and carrying out spray drying to form water extract powder;

and 7, carrying out centrifugal separation on the solid obtained in the step 5 and ethanol according to the weight ratio of 1: 20-30, performing ultrasonic extraction, and centrifuging to separate solid from liquid to obtain ethanol leaching solution;

and 8, evaporating the ethanol in the ethanol leaching liquor at low pressure, and drying to obtain ethanol extract powder.

2. The segmented extraction method of the active ingredients of the moringa leaves according to claim 1, which is characterized in that: and 3, drying the powder in the step 3 by hot air, and storing in a thermostat at 25 ℃.

3. The segmented extraction method of the active ingredients of the moringa leaves according to claim 1, which is characterized in that: the stirring in the step 4 is carried out in a constant-temperature magnetic stirrer, the stirring time is 1-1.5 hours, and the mixture is homogenized under the condition of 300kg/cm2 by a homogenizer.

4. The segmented extraction method of the active ingredients of the moringa leaves according to claim 1, which is characterized in that: in the step 5, the set temperature of the first extraction tank is 45 ℃, the extraction time is 35min, the stirring speed during extraction is 70r/min, the enzyme is immediately inactivated by a boiling water bath for 15min after the time is up, the set temperature of the second extraction tank is 50 ℃, the extraction time is 35min, the enzyme is not stirred during extraction, and the enzyme is immediately inactivated by the boiling water bath for 15min after the time is up.

5. The segmented extraction method of the active ingredients of the moringa leaves according to claim 1, which is characterized in that: the ultrafiltration in the step 6 is ceramic membrane ultrafiltration.

6. The segmented extraction method of the active ingredients of the moringa leaves according to claim 1, which is characterized in that: the power of ultrasonic extraction in the step 7 is 300W, the extraction temperature is 50 ℃, and the extraction time is 45 min.

7. A beverage containing the active ingredients of the moringa leaves is characterized in that water extract powder and ethanol extract powder extracted by the segmented extraction method of the active ingredients of the moringa leaves of any one of claims 1 to 6 are used for preparation, and the beverage comprises the following components in parts by weight:

10-20 parts of water extract powder, 5-10 parts of ethanol extract powder, 10-20 parts of medlar powder, 5-7 parts of sweet osmanthus powder, 8-10 parts of honeysuckle powder and 3-5 parts of liquorice powder.

8. The beverage containing the moringa oleifera active ingredient according to claim 7, wherein: the paint comprises the following components in parts by weight: 15 parts of water extract powder, 7 parts of ethanol extract powder, 15 parts of medlar powder, 6 parts of sweet osmanthus powder, 9 parts of honeysuckle powder and 4 parts of liquorice powder.

9. A method for preparing the beverage containing the active ingredients of the moringa oleifera leaves in claim 7 or 8, wherein the method comprises the following steps: the method comprises the following steps:

firstly, drying medlar, sweet osmanthus, honeysuckle and liquorice by hot air at 45 ℃ respectively, and crushing into powder;

mixing the water extract powder, the ethanol extract powder, the medlar powder, the sweet osmanthus powder, the honeysuckle powder and the liquorice powder in proportion, and uniformly stirring;

and thirdly, packaging the mixed powder according to 10g per package.

Technical Field

The invention relates to the field of moringa oleifera extracts and production methods thereof, and particularly relates to a segmented extraction method of active ingredients of moringa oleifera leaves and a preparation method of drinks and drinks containing the active ingredients of the moringa oleifera leaves.

Background

The moringa oleifera as vegetable and food has the functions of improving nutrition and dietotherapy health care, and meanwhile, the moringa oleifera is widely applied to the aspects of medicine, health care and the like and is known as a living tree. The moringa oleifera contains rich nutrients, is widely applied to improving various diseases, has obvious effect, and is regarded as a saving star of modern medicine by scientists. All parts of moringa oleifera, such as leaves, roots, seeds, bark, fruits, flowers and immature pods, can be used as a medicine. The moringa leaves have good adjuvant treatment effect on hypertension, hyperlipidemia, diabetes and the like. Has effects in relaxing bowels, removing toxic substances, strengthening body constitution, caring skin; increasing the calcium content of bone, enhancing the ability of reluctant excitation, preventing diseases, improving sleep, enhancing memory and delaying aging; the moringa leaf powder contains various amino acids, mineral substances and vitamins, can repair tissue and organism injuries and correct cell functions, and is very beneficial to the growth of children. The moringa aqueous extract contains rich proteins, amino acids and other nutritional ingredients, can supplement proteins and amino acids required by a human body, can reduce the increase of blood sugar caused by streptomicin, reduce the percentage of hemoglobin HBA1C and reduce the lipid peroxidation level, and flavonoids and polyphenol in the ethanol extract in the moringa leaves have the effect of reducing the blood sugar and have high antioxidant activity.

The traditional moringa production and processing is also in simple rough processing, the moringa leaves are usually dried and crushed into powder, and the effective components in the moringa leaves are not effectively extracted and are difficult to be effectively absorbed by a human body; the existing fine processing extraction process has the following defects: only single nutrient substances (such as protein, polysaccharide, fat and the like) can be extracted, the synergistic effect of various nutrient substances cannot be achieved, and the nutrient substances are not extracted completely, so that waste is caused; the existing drying process usually adopts treatment methods such as hot air drying, sun drying or microblog drying, and obviously damages heat-sensitive and photosensitive nutrient substances in moringa leaves; the existing protein extraction mostly adopts a microwave extraction or composite protease extraction process, the extraction rate of microwave extraction is low, usually about thirty percent, while the extraction rate of the composite protease extraction process is slightly high, but the extraction efficiency is still not very high because the cellulose of cell walls is not decomposed and destroyed.

Disclosure of Invention

The technical problem to be solved by the invention is to provide a segmented extraction method of the active ingredients of the moringa leaves, a beverage containing the active ingredients of the moringa leaves and a preparation method of the beverage, wherein the beverage can be used for extracting various effective nutrient substances, has high extraction rate, little damage to heat-sensitive and light-sensitive nutrient substances in the moringa leaves and high extraction rate of protein, and has various effects.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows: a segmented extraction method for active ingredients of moringa leaves is characterized by comprising the following steps:

step 1, feeding the raw material moringa leaves into a heat pump dryer for heat pump drying, wherein the drying temperature is 30-35 ℃, and the drying time is 3-4 hours;

step 2, crushing the dried moringa leaves obtained in the step 1 into powder;

step 3, carrying out hot air drying on the powder obtained in the step 2, wherein the drying temperature is 40-45 ℃, and the drying time is 1-2 hours;

and 4, mixing the powder obtained in the step 3 and deionized water according to the weight ratio of 1: mixing and stirring at the weight ratio of 100-120 and homogenizing;

and 5, regulating the pH value of the homogenized mixed solution to 4-4.5, and carrying out continuous double-irrigation extraction, wherein in the first extraction tank, the ratio of cellulase to the mixed solution 1: adding cellulase in a weight ratio of 100-: adding 100-110 weight percent of composite protease, centrifuging the mixed solution output by the second extraction tank to separate the solid and the liquid to obtain water extract;

step 6, carrying out fine filtration and ultrafiltration on the water extract, and carrying out spray drying to form water extract powder;

and 7, carrying out centrifugal separation on the solid obtained in the step 5 and ethanol according to the weight ratio of 1: 20-30, performing ultrasonic extraction, and centrifuging to separate solid from liquid to obtain ethanol leaching solution;

and 8, evaporating the ethanol in the ethanol leaching liquor at low pressure, and drying to obtain ethanol extract powder.

Preferably, the powder in the step 3 is dried by hot air and then stored in a thermostat at 25 ℃.

Preferably, the stirring in step 4 is carried out in a constant temperature magnetic stirrer, the stirring time is 1-1.5 hours, and the mixture is homogenized at 300kg/cm2 in a homogenizer.

Preferably, in the step 5, the set temperature of the first extraction tank is 45 ℃, the extraction time is 35min, the stirring speed during extraction is 70r/min, the enzyme is immediately deactivated by using a boiling water bath after the time is up, the set temperature of the second extraction tank is 50 ℃, the extraction time is 35min, the stirring is not performed during extraction, and the enzyme is immediately deactivated by using a boiling water bath after the time is up for 15 min.

Preferably, the ultrafiltration in step 6 is ceramic membrane ultrafiltration.

Preferably, the power of the ultrasonic extraction in the step 7 is 300W, the extraction temperature is 50 ℃, and the extraction time is 45 min.

A beverage containing the active ingredients of the moringa leaves is characterized in that water extract powder and ethanol extract powder extracted by the segmented extraction method of the active ingredients of the moringa leaves of any one of claims 1 to 6 are used for preparation, and the beverage comprises the following components in parts by weight:

10-20 parts of water extract powder, 5-10 parts of ethanol extract powder, 10-20 parts of medlar powder, 5-7 parts of sweet osmanthus powder, 8-10 parts of honeysuckle powder and 3-5 parts of liquorice powder.

Preferably, the composition comprises the following components in parts by weight: 15 parts of water extract powder, 7 parts of ethanol extract powder, 15 parts of medlar powder, 6 parts of sweet osmanthus powder, 9 parts of honeysuckle powder and 4 parts of liquorice powder.

A method for preparing the beverage containing the active ingredients of the moringa oleifera leaves in claim 7 or 8, wherein the method comprises the following steps: the method comprises the following steps:

firstly, drying medlar, sweet osmanthus, honeysuckle and liquorice by hot air at 45 ℃ respectively, and crushing into powder;

mixing the water extract powder, the ethanol extract powder, the medlar powder, the sweet osmanthus powder, the honeysuckle powder and the liquorice powder in proportion, and uniformly stirring;

and thirdly, packaging the mixed powder according to 10g per package.

From the above description, the segmented extraction method of the moringa oleifera leaf effective components and the preparation method of the beverage and the beverage containing the moringa oleifera leaf effective components provided by the invention have the following beneficial effects:

the water extraction method and the organic matter extraction method are adopted for extracting the nutrient substances in a segmented manner, so that various effective components such as protein, polysaccharide, amino acid, flavone, polyphenol and the like in the moringa leaves can be extracted, the nutrient substances are completely extracted, the utilization rate is high, and the drink prepared from various nutrient substances has various synergistic effects; the hot pump drying is adopted before crushing, the heat pump drying temperature is low, the damage to heat-sensitive and photosensitive nutrient substances in the moringa leaves is small, the contents of fat and polysaccharide in the moringa leaves are well kept, and the hot air drying is further adopted for a short time after crushing, so that the water content is further reduced, and the extraction rate of effective components in the subsequent steps is improved; the beverage prepared by the method has multiple composite effects, can supplement protein required by a human body, reduce blood sugar increase caused by streptomicin, and reduce lipid peroxidation level, and flavonoid substances and polyphenol in ethanol extract in moringa oleifera leaves have the effect of reducing blood sugar and have high antioxidant activity.

Detailed Description

The invention is further described below by means of specific embodiments.

A segmented extraction method of active ingredients of moringa leaves comprises the following steps:

step 1, feeding the raw material moringa leaves into a heat pump dryer for heat pump drying, wherein the drying temperature is 30-35 ℃, and the drying time is 3-4 hours;

step 2, crushing the dried moringa leaves obtained in the step 1 into powder;

step 3, carrying out hot air drying on the powder obtained in the step 2, wherein the drying temperature is 40-45 ℃, and the drying time is 1-2 hours;

and 4, mixing the powder obtained in the step 3 and deionized water according to the weight ratio of 1: mixing and stirring at the weight ratio of 100-120 and homogenizing;

and 5, regulating the pH value of the homogenized mixed solution to 4-4.5, and carrying out continuous double-irrigation extraction, wherein in the first extraction tank, the ratio of cellulase to the mixed solution 1: adding cellulase in a weight ratio of 100-: adding 100-110 weight percent of composite protease, centrifuging the mixed solution output by the second extraction tank to separate the solid and the liquid to obtain water extract;

step 6, carrying out fine filtration and ultrafiltration on the water extract, and carrying out spray drying to form water extract powder;

and 7, carrying out centrifugal separation on the solid obtained in the step 5 and ethanol according to the weight ratio of 1: 20-30, performing ultrasonic extraction, and centrifuging to separate solid from liquid to obtain ethanol leaching solution;

and 8, evaporating the ethanol in the ethanol leaching liquor at low pressure, and drying to obtain ethanol extract powder.