阅读说明：本技术 可循环使用的血型自动分析装置及血型分析方法 (Recyclable blood type automatic analysis device and blood type analysis method ) 是由 王胜岚 陈楚炎 吴伟铭 于 2019-10-30 设计创作，主要内容包括：本申请涉及医学检测技术领域,尤其涉及可循环使用的血型自动分析装置及血液分析方法,装置包括基座,所述基座上设有多个环形通道、与多个环形通道一一对应连通的溶液进入端以及与每个溶液进入端相连通的样本进入端,所述溶液进入端上设有用于添加反应溶液的溶液入口,所述样本进入端上设有用于添加待测样本的样本入口；所述环形通道在远离所述溶液进入端的一端设有用于识别待测样本与各个环形通道的溶液的反应结果的血型分析装置；所述方法应用该装置。本申请提供的可循环使用的血型自动分析装置及血液分析方法,所述血型自动分析装置可循环使用,操作简单,对操作者的专业程度要求低,且降低了操作者的工作量以及提高血液分析的效率。(The utility model relates to the technical field of medical detection, in particular to a recyclable blood type automatic analysis device and a blood analysis method, the device comprises a base, a plurality of annular channels, a solution inlet end which is communicated with the annular channels in a one-to-one correspondence manner and a sample inlet end which is communicated with each solution inlet end are arranged on the base, a solution inlet for adding reaction solution is arranged on the solution inlet end, and a sample inlet for adding a sample to be detected is arranged on the sample inlet end; one end of the annular channel far away from the solution inlet end is provided with a blood type analysis device for identifying the reaction result of the sample to be detected and the solution of each annular channel; the method applies the device. The application provides a blood type automatic analysis device and blood analysis method of circulated use, but blood type automatic analysis device circulated use, easy operation requires lowly to operator's professional degree, and has reduced operator's work load and has improved blood analysis's efficiency.)

1. The recyclable blood type automatic analysis device is characterized by comprising a base (10), wherein the base (10) is provided with a plurality of annular channels (1), solution inlet ends (2) which are communicated with the annular channels (1) in a one-to-one correspondence manner, and a sample inlet end (3) which is communicated with each solution inlet end (2), the solution inlet ends (2) are provided with solution inlets (21) for adding reaction solutions, and the sample inlet ends (3) are provided with sample inlets (31) for adding samples to be detected; one end of the annular channel (1) far away from the solution inlet end (2) is provided with a blood type analysis device (4) for identifying the reaction result of the sample to be detected and the solution of each annular channel (1).

2. The automatic blood group analysis device according to claim 1, characterised in that each annular channel (1) is provided with a drive pump for driving the liquid flow in the annular channel (1).

3. The automatic blood type analysis device capable of being recycled according to claim 1, further comprising a cleaning mechanism (5), wherein the cleaning mechanism (5) comprises a liquid supply unit (51) and a waste liquid recovery unit (52), the liquid supply unit (51) is communicated with the sample inlet end (3) to inject a cleaning liquid into the annular channel (1), the waste liquid recovery unit (52) is communicated with one end of the annular channel (1) far away from the solution inlet end (2) to collect the cleaned solution, and the waste liquid recovery unit (52) is communicated with the annular channel (1) through a three-way reversing valve.

4. The automatic blood group analysis device capable of being recycled according to claim 3, wherein the liquid supply unit (51) comprises a liquid storage chamber for storing a cleaning liquid, a liquid inlet pump for providing output power for the cleaning liquid, and a first electromagnetic valve arranged between the sample inlet end (3) and the liquid inlet pump.

5. The automatic blood group analysis device according to claim 1, wherein a second solenoid valve is provided in the solution inlet end (2) and a third solenoid valve is provided in the sample inlet end (3).

6. The automatic blood group analysis device according to claim 1, wherein the annular channel (1) is provided with heating means for heating the liquid in the channel.

7. The automatic blood type analysis device capable of being recycled according to claim 1, wherein the heating mechanism is an electric heating patch attached to the outer wall of the annular channel (1), and heating wires capable of being electrically heated are distributed on the electric heating patch.

8. The automatic blood group analysis device capable of being recycled according to any one of claims 1 to 7, wherein the sample inlet (31) is in a frustum shape which is gradually reduced from top to bottom, and the bottom of the sample inlet (31) is provided with a plurality of parallel square inlets which are communicated with the solution inlets (21) in a one-to-one correspondence manner.

9. A blood analysis method, comprising:

preheating: the heating mechanism (6) respectively heats the four annular channels (1) to preset temperatures;

addition of the reaction solution: respectively introducing four reaction solutions, namely a first solution, a second solution, a third solution and a fourth solution, into the four annular channels (1) from the solution inlet end (2);

adding a sample to be tested: dividing a sample to be detected into four parts from a sample inlet end (3) and respectively introducing the four parts into the four annular channels (1);

mixing and checking: when the sample to be tested and four solutions of the first solution, the second solution, the third solution and the fourth solution respectively circulate in the four annular channels (1) to be fully mixed, the blood type analysis device (4) detects the coagulation condition of the solutions in the annular channels (1) and the sample to be tested and obtains a blood analysis result;

waste liquid discharge: respectively discharging the solution detected by the four annular channels (1) and the sample to be detected to a waste liquid recovery unit (52) through a reversing valve;

cleaning: and introducing cleaning liquid into the four annular channels (1) for multiple times from the sample inlet end (3) to clean the annular channels (1), and discharging the liquid in the annular channels (1) to a waste liquid recovery unit (52) after each cleaning.

10. The method for blood analysis according to claim 9, wherein the flow rates of the four solutions during the passing through the solution entrance ends (2) are controlled to be 0.5 to 1 μ l/s, respectively;

controlling the flow rate of the sample to be detected and the four solutions to be detected to be 1-1.5 mu l/s when the samples to be detected and the four solutions are mixed in the four annular channels (1);

when the blood group analysis device (4) starts to detect, controlling the flow rate of the solution in the annular channel (1) to be 1.5-2 mu l/s;

during the cleaning process, the flow rate of the solution in the annular channel (1) is controlled to be 5-6 mu l/s.

[ technical field ] A method for producing a semiconductor device

The application relates to the technical field of blood type detection, in particular to a recyclable blood type automatic analysis device and a blood type analysis method.

[ background of the invention ]

Blood analysis is one of the conventional medical testing items, including detection experiments such as positive typing, negative typing, anti-human globulin (direct method), anti-human globulin (indirect method) and the like.

At present, the process of preparing a sample in blood analysis is complex, the detection time is long, the requirement on the operation capability of personnel is high, and the sample reagent is easily polluted in the detection process.

[ summary of the invention ]

The utility model provides a blood type automatic analysis device and blood type analysis method of circulated use, but blood type automatic analysis device circulated use, easy operation requires lowly to operator's professional degree, and has reduced operator's work load and has improved blood analysis's efficiency.

The application is realized by the following technical scheme:

the recyclable blood type automatic analysis device comprises a base, wherein the base is provided with a plurality of annular channels, solution inlet ends which are communicated with the annular channels in a one-to-one correspondence mode, and a sample inlet end which is communicated with each solution inlet end; and one end of the annular channel far away from the solution inlet end is provided with a blood type analysis device for identifying the reaction result of the sample to be detected and the solution of each annular channel.

According to the recyclable blood type automatic analysis device, each annular channel is provided with the driving pump for driving the liquid in the annular channel to flow.

The recyclable blood type automatic analysis device further comprises a cleaning mechanism, wherein the cleaning mechanism comprises a liquid supply unit and a waste liquid recovery unit, the liquid supply unit is communicated with the sample inlet end to fill the annular channel with the cleaning liquid, the waste liquid recovery unit is communicated with one end, far away from the solution inlet end, of the annular channel to collect the cleaned solution, and the waste liquid recovery unit is communicated with the annular channel through a three-way reversing valve.

According to the blood type automatic analysis device capable of being recycled, the liquid supply unit comprises a liquid storage cavity for storing the cleaning liquid, a liquid inlet pump for providing output power for the cleaning liquid and a first electromagnetic valve arranged between the sample inlet end and the liquid inlet pump.

According to the automatic blood type analysis device capable of being recycled, the second electromagnetic valve is arranged in the solution inlet end, and the third electromagnetic valve is arranged in the sample inlet end.

According to the blood type automatic analysis device capable of being recycled, the annular channel is provided with a heating mechanism for heating liquid in the channel.

According to the blood type automatic analysis device capable of being recycled, the heating mechanism is an electric heating paste pasted on the outer wall of the annular channel, and electric heating wires capable of being electrified and heated are distributed on the electric heating paste.

According to the recyclable blood type automatic analysis device, the sample inlet is in a frustum shape gradually becoming small from top to bottom, the bottom of the sample inlet is provided with a plurality of parallel square inlets, and the square inlets are communicated with the solution inlet in a one-to-one correspondence mode.

The present application also provides a method of blood type analysis comprising:

preheating: the heating mechanism respectively heats the four annular channels to a preset temperature;

addition of the reaction solution: respectively introducing four reaction solutions, namely a first solution, a second solution, a third solution and a fourth solution, into the four annular channels from solution inlet ends;

adding a sample to be tested: dividing a sample to be detected into four parts from a sample inlet end and respectively introducing the four parts into the four annular channels;

mixing and checking: after the sample to be tested and four reaction solutions of the first solution, the second solution, the third solution and the fourth solution respectively circulate in the four annular channels to be fully mixed, the blood type analysis device detects the coagulation condition of the solution in each annular channel and the sample to be tested and obtains a blood analysis result;

waste liquid discharge: respectively discharging the solution detected by the four annular channels and the sample to be detected to a waste liquid recovery unit through a reversing valve;

cleaning: and introducing cleaning liquid into the four annular channels for multiple times from the sample inlet end to clean the annular channels, and discharging the liquid in the annular channels to the waste liquid recovery unit after each cleaning.

In the blood type analysis method, the flow rate of the four solutions in the process of respectively introducing the four solutions from the solution inlet end is controlled to be 0.5-1 mu l/s;

controlling the flow rate of the sample to be detected and the four solutions to be 1-1.5 mul/s when the samples to be detected and the four solutions are mixed in the four annular channels respectively;

when the blood type analysis device starts to detect, controlling the flow rate of the solution in the annular channel to be 1.5-2 mul/s;

during the cleaning process, the flow rate of the solution in the annular channel is controlled to be 5-6 mul/s.

Compared with the prior art, the method has the following advantages:

but blood type automatic analysis device recycles, easy operation, requires lowly to operator's professional degree, and has reduced operator's work load and improved blood analysis's efficiency.

The blood type analysis method uses the blood type automatic analysis device, because the sample to be measured and the reaction solution are in the diameter minimum the circular pipeline can circulate intensive mixing, the required sample volume is very little and need not dilute, and sensitivity is high moreover, and the specificity is strong, but heating mechanism automatic incubation, the testing process need not the centrifugation, and is simple and convenient.

[ description of the drawings ]

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

Fig. 1 is a schematic perspective view of an automatic blood type analysis device capable of being recycled according to an embodiment of the present application.

Fig. 2 is a front view of an embodiment of the present application, in which a dotted line portion is a schematic distribution diagram of each channel.

Fig. 3 is a sectional view a-a of fig. 2.

[ detailed description ] embodiments

In order to make the technical problems, technical solutions and advantageous effects solved by the present application more clear and obvious, the present application is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.

As shown in fig. 1 to 3, an embodiment of the present application provides a recyclable blood type automatic analysis device, including a base 10, where the base 10 is provided with a plurality of annular channels 1, solution inlet ends 2 communicated with the plurality of annular channels 1 in a one-to-one correspondence manner, and a sample inlet end 3 communicated with each solution inlet end 2, the solution inlet end 2 is provided with a solution inlet 21 for adding a reaction solution, and the sample inlet end 3 is provided with a sample inlet 31 for adding a sample to be detected; and one end of the annular channel 1, which is far away from the solution inlet end 2, is provided with a blood type analysis device 4 for identifying the reaction result of the sample to be detected and the solution of each annular channel 1. Wherein the diameter of the annular channel 1 is 1-2mm, preferably 1.5 mm.

But automatic analytical equipment of blood group of circulated use can carry out detection experiments such as normal design blood group detection, anti-human globulin (direct method), anti-human globulin (indirect method), is particularly useful for normal design blood group detection, anti-design blood group detection, judges the blood group through the agglutination condition of analysis sample to be measured and reaction solution, optionally, thereby blood group analytical equipment is through whether the sample that analysis awaits measuring takes place the agglutination with different reaction solutions and reachs the analysis result, for example can use the machine vision interpretation method and the device that is used for blood group analysis that patent publication number is CN102446353B to carry out the identification analysis, can also judge through the magnetic bead of identification mark antibody that there is cell agglutination to carry out the analysis to the sample that awaits measuring. In another embodiment, the blood group analysis device can be replaced by a manual device, the annular channel is arranged as a transparent pipeline, and the user observes the agglutination condition in the annular channel so as to perform interpretation to obtain the analysis result.

But blood type automatic analysis device recycles, easy operation, requires lowly to operator's professional degree, and has reduced operator's work load and improved blood analysis's efficiency.

The recyclable blood type automatic analysis device further comprises a cleaning mechanism 5, wherein the cleaning mechanism 5 comprises a liquid supply unit 51 and a waste liquid recovery unit 52, the liquid supply unit 51 is communicated with the sample inlet end 3 to inject cleaning liquid into the annular channel 1, the waste liquid recovery unit 52 is communicated with one end, away from the solution inlet end 2, of the annular channel 1 to collect cleaned solution, and the waste liquid recovery unit 52 is communicated with the annular channel 1 through a three-way reversing valve. Wherein the cleaning liquid is purified water. The liquid supply unit 51 comprises a liquid storage cavity for storing cleaning liquid, a liquid inlet pump for providing output power for the cleaning liquid, and a first electromagnetic valve arranged between the sample inlet end 3 and the liquid inlet pump. The first electromagnetic valve is used for controlling the on and off of the cleaning liquid in the liquid supply unit 51.

More specifically, the waste liquid recovery unit 52 includes a collection container and output pipes formed to extend toward the lower portion of each annular channel 1, and each output pipe is communicated with the collection container, wherein the diameter of the output pipe is 0.5 to 1.5mm, preferably 1 mm; the length is 5-15mm, preferably 10 mm. In one embodiment, the junction of the output conduit is threaded and the collection container is threaded into the junction of the output conduit to form a removable structure to facilitate cleaning of waste fluids. Furthermore, the output pipeline and the annular channel 1 form a T-shaped channel, and the annular channel 1 can selectively form a closed-loop channel or an open-loop channel for outputting to the waste liquid recovery unit 52 through a three-way reversing valve.

And a heating mechanism for heating liquid in the channel is arranged on the annular channel 1. Specifically, the heating mechanism is an electric heating patch attached to the outer wall of the annular channel 1, and electric heating wires capable of being electrified and heated are distributed on the electric heating patch. Of course, the heating mechanism further comprises a power supply and a heating circuit for controlling the heating temperature of the heating wire. The heating mechanism may heat the annular channel 1 to 20-40 c, preferably 25 c. Through heating mechanism can carry out the incubation to the sample that awaits measuring, through the analysis experiment of different grade type of different temperature switch adaptation to reach more excellent experimental effect.

In this embodiment, each annular channel 1 is provided with a driving pump for driving the liquid in the annular channel 1 to flow, and the driving pump is a micro pump and can be used for controlling the flow rate of the solution in the channel.

A second electromagnetic valve is arranged in the solution inlet end 2, and a third electromagnetic valve is arranged in the sample inlet end 3. When the second electromagnetic valve is in an open state, the reaction solution enters the solution inlet end 2, the second electromagnetic valve is closed after the reaction solution enters, when the third electromagnetic valve is in an open state, the sample to be detected enters the solution inlet end 2, and the third electromagnetic valve is closed after the reaction solution enters. The second electromagnetic valve and the third electromagnetic valve can ensure that the annular channel is in a sealed state after the reaction solution and the sample to be detected enter the annular channel 1, can effectively prevent impurities from entering the annular channel 1 to pollute the solution, and further ensures the accuracy of detection and analysis.

Preferably, the sample inlet 31 is in a truncated pyramid shape gradually decreasing from top to bottom, and the bottom of the sample inlet 31 is provided with a plurality of parallel square inlets, and the square inlets are in one-to-one correspondence communication with the solution inlets 21. The plurality of parallel square inlets are close to each other, when a sample to be detected is added, the sample to be detected can be evenly distributed into the square inlets, so that the purpose of adding the sample to be detected into each annular channel 1 is achieved, and the method has the advantages that the sample to be detected can be automatically distributed only by adding the sample once.

The embodiment also provides a blood type analysis method, which applies the recyclable blood type automatic analysis device, and comprises the following steps:

preheating: the heating mechanism 6 respectively heats the four annular channels 1 to a preset temperature, specifically to 20-40 ℃, and preferably to 25 ℃;

addition of the reaction solution: respectively introducing four reaction solutions, namely a first solution, a second solution, a third solution and a fourth solution, into the four annular channels 1 from the solution inlet end 2, and closing the solution inlet end 2;

adding a sample to be tested: dividing a sample to be detected into four parts from a sample inlet end 3, respectively introducing the four parts into the four annular channels 1, and closing the sample inlet end 3;

mixing and checking: after the sample to be tested and four reaction solutions of the first solution, the second solution, the third solution and the fourth solution respectively circulate in the four annular channels 1 to be fully mixed, the blood type analysis device 4 detects the coagulation condition of the solution in each annular channel 1 and the sample to be tested and obtains a blood analysis result;

waste liquid discharge: respectively discharging the solution detected by the four annular channels 1 and the sample to be detected to a waste liquid recovery unit 52 through a reversing valve;

cleaning: cleaning liquid (such as pure water) is introduced into the four annular channels 1 from the sample inlet end 3 for a plurality of times to clean the annular channels 1, and the liquid in the annular channels 1 is discharged to the waste liquid recovery unit 52 after each cleaning.

Preferably, in the process of implementing the blood type analysis method, the flow rate of the four reaction solutions which are respectively introduced from the solution inlet end 2 is controlled to be 0.5-1 mul/s, so that the reaction solutions can enter more smoothly, and a sample to be detected can be driven to enter during sample introduction; controlling the flow rate of the sample to be detected and the four solutions to be 1-1.5 mul/s when the sample to be detected and the four solutions are mixed in the four annular channels 1 respectively, and increasing the flow rate to fully mix the sample to be detected and the reaction solution so as to promote the reaction between the sample to be detected and the reaction solution; when the blood type analysis device 4 starts to perform detection analysis, the flow rate of the solution in the annular channel 1 is controlled to be 1.5-2 μ l/s, so that the blood type analysis device 4 can perform sampling analysis more quickly and obtain a detection result; in the cleaning process, the flow rate of the solution in the annular channel 1 is controlled to be 5-6 mul/s, which is beneficial to flushing the pipeline more quickly and effectively. The rupture of cells in the solution can be effectively prevented by controlling the flow rate, wherein a micro pump or a solenoid valve can be selected to control the flow rate.

In the use process, the blood type automatic analysis device capable of being recycled is switched at different speeds, so that the detection process is always in a high-efficiency and reasonable state. In addition, different temperatures of the four annular channels are configured through different detection experiments, so that a sample to be detected and a reaction solution are at the optimal reaction temperature, the bonding strength of the sample to be detected and the reaction solution is promoted, and the reaction result is more stable and obvious.

As mentioned above, the blood type analyzer can determine whether there is agglutination of cells by using magnetic beads for identifying labeled antibodies, and the specific implementation process is as follows:

(1) the 4 circular channels through the blood typing device 4 are each a channel with 3 layers of microfiltration membranes, the pore size of the channel is 10-25 μm, and single red blood cells and free antibodies can be allowed to pass through the microfiltration membranes at a flow rate of 1.5-2 μ l/s, and agglutinated red blood cells are excluded. The effect of microfiltration membrane is with agglutinate and the red blood cell separation of non-agglutination, make blood group analysis device 4 can detect agglutinate red blood cell more sensitively, wherein, this part is equipped with the passageway of microfiltration membrane and is detachable construction (if set this part be equipped with the passageway of microfiltration membrane into with the passageway of the different apertures of coupling part, in order to form pluggable bushing structure), the filtration effect can reduce after the microfiltration membrane uses many times, can regularly change the new passageway that has the microfiltration membrane through detachable construction, guarantee the stability of device.

(2) The agglutination of the red blood cells is bridged through an antibody, the agglutinated red blood cells contain the antibody marked with magnetic beads, and the blood type analysis device detects whether a magnetic field exists at the position of the micro-filtration membrane or not so as to judge whether agglutination red blood cells exist or not and further judge whether reaction occurs or not, and then converts a magnetic field signal into an electric signal output result.

Wherein, the washing is carried out after the blood group analytical equipment 4 detects the analysis and finishes, and in the cleaning process, can make the red blood cell of exclusion at the microfiltration membrane expand brokenly and be washed clean when the pipeline is washed to pure water, and the microfiltration membrane does not have the residue after the washing, avoids influencing the analysis of blood group analytical equipment 4 to the experiment next time.

In this embodiment, the blood type analysis method can be applied to detection experiments of positive typing, negative typing, anti-human globulin (direct method), and anti-human globulin (indirect method). The following is specifically described based on the above blood type analysis method, in which the experimental result is judged by the blood type analysis device 4:

first, positive sizing experiment

a. The heating mechanism 6 heats the four annular channels 1 to 25 +/-1 ℃, and the first solution, the second solution, the third solution and the fourth solution are sequentially an A antibody, a B antibody, a RhD antibody and normal saline.

b. The sample to be detected is whole blood.

c. The titer of the A antibody is 150-200, preferably 160; the titer of the B antibody is 150-200, preferably 180; the titer of the RhD antibody is 150-200, preferably 170.

d. The results of the experiment were interpreted as follows:

solution for reacting with sample to be tested	Results of the experiment
		Antibody reaction	Blood group A
B antibody reaction	Blood of type B
		Both antibody A and antibody B react	AB type blood
The A antibody and the B antibody are not reacted	Blood of type O

Wherein, if the RhD antibody reacts, the RhD is positive, and if the RhD antibody does not react, the RhD is negative; the reaction of the normal saline is invalid, and the reaction of the normal saline is not valid.

Second, reverse sizing experiment

a. The heating mechanism 6 heats the four annular channels 1 to 25 +/-1 ℃, and the first solution, the second solution, the third solution and the fourth solution are sequentially an A cell reagent, a B cell reagent, an O cell reagent and normal saline.

b. The sample to be detected is plasma.

c. The concentration of the A cell reagent is 2-4%; the concentration of the B cell reagent is 2-4%; the concentration of the O cell reagent is 2-4%.

d. The results of the experiment were interpreted as follows:

solution for reacting with sample to be tested	Results of the experiment
		A cell reagent reaction	Blood of type B
B cell reagent reaction	Blood group A
		Both A cell reagent and B cell reagent react	Blood of type O
The A cell reagent and the B cell reagent do not react	AB type blood

Wherein, the O cell reagent reaction needs further anti-human globulin experiment, and the O cell reagent is normal without reaction; the physiological saline reacts immediately and the experiment is invalid, and the physiological saline does not react immediately and the experiment is valid.

Anti-human globulin test (indirect method)

a. Heating mechanism6, respectively heating the four annular channels 1 to 37 +/-1 ℃, wherein the first solution, the second solution, the third solution and the fourth solution are sequentially O^ⅠErythrocyte reagent, O^ⅡErythrocyte reagent, O^ⅢErythrocyte reagent, and physiological saline.

b. The sample to be detected is plasma.

c、O^ⅠThe concentration of the erythrocyte reagent is 2-4%; o is^ⅡThe concentration of the erythrocyte reagent is 2-4%; o is^ⅢThe concentration of the erythrocyte reagent is 2-4%.

d. The results of the experiment were interpreted as follows:

the experiment is used for checking whether free incomplete antibodies exist in serum, the experiment is invalid when the physiological saline reacts, and the experiment is valid when the physiological saline does not react.

Anti-human globulin test (direct method)

a. The heating mechanism 6 heats the four annular channels 1 to 37 +/-1 ℃, and the first solution, the second solution, the third solution and the fourth solution are sequentially anti-IgG antibody, anti-C3 d antibody, anti-IgG + C3d antibody and normal saline.

b. The sample to be detected is whole blood.

c. The titer of anti-IgG antibodies is 5-10, preferably 8; the titer of anti-C3 d antibody is 4-8, preferably 6; the titer of anti-IgG + C3d antibody was 8+ 6.

d. The results of the experiment were interpreted as follows:

the experiment is used for detecting the incomplete antibody on the surface of the erythrocyte, the physiological saline reacts immediately and the experiment is invalid, and the physiological saline does not react immediately and the experiment is valid.

But blood type automatic analysis device recycles, easy operation, requires lowly to operator's professional degree, and has reduced operator's work load and improved blood analysis's efficiency. The blood type analysis method uses the blood type automatic analysis device, because the sample to be measured and the reaction solution are in the diameter minimum the circular pipeline can circulate intensive mixing, the required sample volume is very little and need not dilute, and sensitivity is high moreover, and the specificity is strong, but heating mechanism automatic incubation, the testing process need not the centrifugation, and is simple and convenient.

The foregoing is illustrative of one or more embodiments provided in connection with the detailed description and is not intended to limit the disclosure to the particular forms disclosed. Similar or identical methods, structures, etc. as used herein, or several technical inferences or substitutions made on the concept of the present application should be considered as the scope of the present application.