阅读说明：本技术 一种降低烟气自由基含量的卷烟及其制备方法 (Cigarette capable of reducing free radical content in smoke and preparation method thereof ) 是由 李凌 卢志菁 杨叶 梁冠新 于 2019-11-08 设计创作，主要内容包括：本发明属于卷烟技术领域,具体涉及一种降低烟气自由基含量的卷烟及其制备方法。该卷烟将玉竹醇提、酶解、水提后得到的提取物与羧甲基壳寡糖复配加入烟丝和/或滤嘴中,可以显著提高玉竹提取物的稳定性,达到显著提高自由基清除率,提高卷烟的抽吸品质的效果；同时该卷烟制备方法步骤简单,适用于工业化大规模生产。(The invention belongs to the technical field of cigarettes, and particularly relates to a cigarette capable of reducing the content of free radicals in smoke and a preparation method thereof. According to the cigarette, the extract obtained by alcohol extraction, enzymolysis and water extraction of the polygonatum odoratum is compounded with the carboxymethyl chitosan oligosaccharide and then added into tobacco shreds and/or filter tips, so that the stability of the polygonatum odoratum extract can be remarkably improved, and the effects of remarkably improving the clearance rate of free radicals and improving the smoking quality of the cigarette are achieved; meanwhile, the cigarette preparation method has simple steps and is suitable for industrial large-scale production.)

1. A cigarette for reducing the content of free radicals in smoke is characterized in that a cigarette additive consisting of a polygonatum extract and carboxymethyl chitosan oligosaccharide in a ratio of 1 (0.2-2) is added into cigarette tobacco shreds and/or a filter tip;

wherein the preparation method of the polygonatum odoratum extract comprises the following steps:

s1, drying and crushing polygonatum odoratum into fine powder, adding an ethanol solution with the concentration of 80-100% (v/v), carrying out ultrasonic extraction for 15-60 min, filtering to obtain filter residues, concentrating the filtrate, and drying to obtain an alcohol extract of the polygonatum odoratum;

s2, adding the filter residue obtained in the step S1 into water, adding a compound enzyme, and carrying out enzymolysis for 15-60 min at the temperature of 25-30 ℃ and under the condition that the pH value is 5-7 to obtain an enzymolysis liquid;

s3, carrying out reflux extraction on the enzymolysis liquid obtained in the step S2 for 30-90 min, filtering, adding absolute ethyl alcohol into filtrate, shaking and mixing, precipitating at 1-4 ℃ for 8-16 h, centrifuging and drying to obtain a polygonatum aqueous extract;

and S4, mixing the alcohol extract of the polygonatum obtained in the step S1 with the water extract of the polygonatum obtained in the step S3 to obtain the polygonatum.

2. The cigarette as claimed in claim 1, wherein the cigarette additive comprises polygonatum odoratum extract and carboxymethyl chitosan oligosaccharide in a ratio of 1 (0.2-1).

3. The cigarette according to claim 2, wherein the cigarette additive consists of polygonatum odoratum extract and carboxymethyl chitosan oligosaccharide in a ratio of 1: 0.5.

4. The cigarette according to claim 1, wherein in step S2, the complex enzyme is composed of papain and cellulase in a weight ratio of 1 (1-5).

5. The cigarette according to claim 1, wherein in step S2, the amount of the complex enzyme is 4-8 g/L.

6. The cigarette according to claim 1, wherein in the step S1, the power of the ultrasonic extraction is 100-500W.

7. The cigarette according to claim 1, wherein in step S3, the temperature of the reflux extraction is 70-85 ℃.

8. The cigarette according to claim 1, wherein the additive is added in the tobacco shreds in an amount of 0.02-1% by weight of the tobacco shreds.

9. The cigarette according to claim 1, wherein the additive amount of the cigarette additive in the filter is 1-10% of the weight of the filter.

10. A method of manufacturing a cigarette according to any one of claims 1 to 9, comprising the steps of:

preparing a polygonatum extract, adding the obtained polygonatum extract and carboxymethyl chitosan oligosaccharide into water, dissolving to prepare an aqueous solution containing 0.1-1% of a cigarette additive, adding the aqueous solution into cigarette tobacco shreds and/or filter tips, drying, balancing, and making cigarettes.

Technical Field

The invention belongs to the technical field of cigarettes. More particularly, relates to a cigarette for reducing the content of free radicals in smoke and a preparation method thereof.

Background

The cigarette smoke contains thousands of compounds, free radicals are one of the compounds, most of the free radicals are harmful to human bodies, can directly damage cell membranes and biological macromolecules, can consume antioxidant enzymes in human bodies, causes the disturbance of antioxidant enzyme systems and the reduction of enzyme activity in the human bodies, and is a main reason for the increase of the incidence of diseases such as respiratory diseases, cardiovascular diseases, oral diseases, tumors and the like.

At present, particulate free radicals with larger molecular weight and higher stability in smoke are removed mainly by methods of improving the interception and adsorption efficiency of a filter tip, increasing the air permeability of cigarette paper and the like; gas-phase free radicals with small weight and poor stability in smoke are removed mainly by adding a free radical inhibitor, a free radical scavenger, a natural antioxidant, a traditional Chinese medicine extract, trace elements, natural minerals and the like. For example, Chinese patent application CN102423125A discloses a tobacco product containing vitamin C compounds and its application, the tobacco product has more vitamin C in the main stream smoke by adding a certain amount of vitamin C compounds, thereby achieving the purpose of reducing free radicals in the smoke, and simultaneously reducing the pungent taste of the cigarette, but the vitamin C antioxidants have poor stability, are very easy to be naturally oxidized, and the ability of removing free radicals after long-time storage can be obviously reduced.

Therefore, the cigarette which has strong stability and can obviously reduce the free radical content of the smoke and the preparation method thereof are urgently needed.

Disclosure of Invention

The invention aims to solve the technical problem of overcoming the defect and the defect of poor stability of adding an antioxidant into cigarettes in the prior art and providing the cigarettes with strong stability and capability of obviously reducing the content of free radicals in smoke.

The invention also aims to provide a preparation method of the cigarette.

The above purpose of the invention is realized by the following technical scheme:

a cigarette for reducing free radical content in smoke is prepared by adding cigarette additive comprising rhizoma Polygonati Odorati extract and carboxymethyl chitosan oligosaccharide in an amount of 1 (0.2-2) into tobacco shred and/or filter tip of cigarette;

wherein the preparation method of the polygonatum odoratum extract comprises the following steps:

s1, drying and crushing polygonatum odoratum into fine powder, adding an ethanol solution with the concentration of 80-100% (v/v), carrying out ultrasonic extraction for 15-60 min, filtering to obtain filter residues, concentrating the filtrate, and drying to obtain an alcohol extract of the polygonatum odoratum;

s2, adding the filter residue obtained in the step S1 into water, adding a compound enzyme, and carrying out enzymolysis for 15-60 min at the temperature of 25-30 ℃ and under the condition that the pH value is 5-7 to obtain an enzymolysis liquid;

s3, carrying out reflux extraction on the enzymolysis liquid obtained in the step S2 for 30-90 min, filtering, adding absolute ethyl alcohol into filtrate, shaking and mixing, precipitating at 1-4 ℃ for 8-16 h, centrifuging and drying to obtain a polygonatum aqueous extract;

and S4, mixing the alcohol extract of the polygonatum obtained in the step S1 with the water extract of the polygonatum obtained in the step S3 to obtain the polygonatum.

According to the invention, the extract of polygonatum odoratum after alcohol extraction, enzymolysis and water extraction is added into tobacco shreds and/or filter tips, the polygonatum odoratum extract contains active ingredients such as flavonoids and polysaccharides, so that the content of free radicals in smoke can be remarkably reduced, and volatile fragrant substances can improve the smoking quality of cigarettes. However, the active ingredients in the polygonatum odoratum extract are unstable and are very easily affected by the external environment during the standing process, and after a period of time, the clearance rate of free radicals is remarkably reduced, and the stability is low. Aiming at the problems, the inventor surprisingly discovers that the polygonatum odoratum extract and the carboxymethyl chitosan oligosaccharide are compounded to be used as a cigarette additive to be added into cigarette tobacco shreds and/or filter tips, so that the stability of the polygonatum odoratum extract can be remarkably improved, and the free radical clearance rate can reach about 20% after the cigarette is placed for 12 months. The inventor believes that the mechanism of the method is that the carboxymethyl chitosan oligosaccharide forms a layer of protective film outside the active ingredients of the polygonatum extract and is combined with the active ingredients through hydrogen bonds to achieve the effect of stabilizing the active ingredients of the polygonatum extract.

Preferably, the cigarette additive consists of a polygonatum extract and carboxymethyl chitosan oligosaccharide in a ratio of 1 (0.2-1).

More preferably, the cigarette additive consists of the polygonatum extract and the carboxymethyl chitosan oligosaccharide in a ratio of 1: 0.5.

Further, in the step S2, the complex enzyme is composed of papain and cellulase in a weight ratio of 1 (1-5).

Preferably, in the step S2, the complex enzyme is composed of papain and cellulase in a weight ratio of 1 (1-3).

More preferably, in the step S2, the complex enzyme consists of papain and cellulase in a weight ratio of 1: 3.

Furthermore, in the step S2, the addition amount of the complex enzyme is 4-8 g/L.

Preferably, in the step S2, the addition amount of the complex enzyme is 4-6 g/L.

More preferably, in the step S2, the addition amount of the complex enzyme is 5 g/L.

When water extraction is carried out on polygonatum, due to the existence of certain viscous components in polygonatum, filter paper is easy to block during filtration, so that the problems of slow filtration and low polysaccharide extraction efficiency are caused. The inventor finds that the filter residue after alcohol extraction of the polygonatum odoratum is subjected to enzymolysis by using the complex enzyme and then subjected to water extraction, so that the problems of filter blockage and slowness can be avoided, the cell wall of the polygonatum odoratum is damaged, the release of active ingredients is promoted, and the extraction rate of the active ingredients of the polygonatum odoratum is remarkably improved.

Further, in the step S1, the power of ultrasonic extraction is 100-500W.

Preferably, in the step S1, the power of the ultrasonic extraction is 200-500W.

More preferably, in step s1, the power of the ultrasonic extraction is 300W.

Furthermore, in the step S3, the temperature of the reflux extraction is 70-85 ℃.

Preferably, in the step S3, the temperature of the reflux extraction is 70-80 ℃.

More preferably, in step s3, the temperature of the reflux extraction is 80 ℃.

Further, in the step s1, the addition amount of the ethanol solution is 5 to 20 times (weight to volume ratio) of the weight of the polygonatum fine powder.

Furthermore, in the step S2, the addition amount of the water is 5-20 times (weight to volume ratio) of the weight of the filter residue.

Further, in the step S3, the addition amount of the absolute ethyl alcohol is 4-6 times (volume ratio) of the volume of the filtrate.

Furthermore, the adding amount of the cigarette additive in the cut tobacco is 0.02-1% of the weight of the cut tobacco.

Preferably, the addition amount of the cigarette additive in the cut tobacco is 0.02-0.5% of the weight of the cut tobacco.

More preferably, the addition amount of the cigarette additive in the cut tobacco is 0.2% of the weight of the cut tobacco.

Further, the adding amount of the cigarette additive in the filter tip is 1-10% of the weight of the filter tip.

Preferably, the adding amount of the cigarette additive in the filter tip is 1-5% of the weight of the filter tip.

More preferably, the cigarette additive is added into the filter in an amount of 2% by weight of the filter.

In addition, the invention also provides a preparation method of the cigarette, which comprises the following steps:

preparing a polygonatum extract, adding the obtained polygonatum extract and carboxymethyl chitosan oligosaccharide into water, dissolving to prepare an aqueous solution containing 0.1-1% of a cigarette additive, adding the aqueous solution into cigarette tobacco shreds and/or filter tips, drying, balancing, and making cigarettes.

The invention has the following beneficial effects:

(1) according to the cigarette for reducing the content of free radicals in smoke, the extract obtained by alcohol extraction, enzymolysis and water extraction of polygonatum odoratum is compounded with carboxymethyl chitosan oligosaccharide and added into tobacco shreds and/or filter tips, so that the stability of the polygonatum odoratum extract can be remarkably improved, and the effects of remarkably improving the clearance rate of the free radicals and improving the smoking quality of the cigarette are achieved.

(2) The preparation method of the cigarette for reducing the free radical content in the smoke has simple steps and can be applied to industrial large-scale production.

Detailed Description

The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.

Unless otherwise indicated, reagents and materials used in the following examples are commercially available.