Glass slide structure suitable for confocal microscope
阅读说明：本技术 适用于共聚焦显微镜的玻片结构 (Glass slide structure suitable for confocal microscope ) 是由 李蒙 李媛媛 李树营 陶林 曲梅 喻敏 李学文 于 2019-09-27 设计创作，主要内容包括：本发明公开了一种适用于共聚焦显微镜的玻片结构,包括：中间玻片,所述中间玻片上设有若干第一通孔；盖玻片和载玻片,所述盖玻片、所述中间玻片和所述载玻片依次层叠设置；弹性材料制成的密封圈,设于所述第一通孔内且厚度略大于所述中间玻片的厚度,使得所述盖玻片、所述中间玻片和所述载玻片依次贴合时所述密封圈的上、下侧分别与所述盖玻片、所述载玻片抵接。通过设置密封圈,使用时先把中间玻片放在载玻片上,然后把密封圈放在第一通孔内,然后把观察物置于密封圈内,然后盖上盖玻片,再使用外置的夹紧件夹紧盖玻片、中间玻片和载玻片,由于密封圈的上、下侧分别与所述盖玻片、所述载玻片抵接,透明剂和荧光染料不容易溢出。(The invention discloses a glass slide structure suitable for a confocal microscope, which comprises: the middle glass slide is provided with a plurality of first through holes; the cover glass, the intermediate glass and the glass slide are sequentially stacked; and the sealing ring is made of elastic material, is arranged in the first through hole, and has a thickness slightly larger than that of the intermediate glass slide, so that the upper side and the lower side of the sealing ring are respectively abutted against the cover glass and the glass slide when the cover glass, the intermediate glass slide and the glass slide are sequentially attached. Through setting up the sealing washer, put middle slide on the glass slide earlier during the use, then put the sealing washer in first through-hole, then arrange the observation thing in the sealing washer, then cover the glass slide, use external clamping piece clamp cover glass, middle slide and glass slide again, because the upper and lower side of sealing washer respectively with the cover glass slide butt, the sanitizer and fluorochrome are difficult to spill over.)
1. A glass slide structure suitable for a confocal microscope is characterized in that: the method comprises the following steps:
the middle glass sheet is provided with a plurality of first through holes;
the cover glass, the intermediate glass and the glass slide are sequentially stacked;
and the sealing ring is made of elastic material, is arranged in the first through hole, and has a thickness slightly larger than that of the intermediate glass slide, so that the upper side and the lower side of the sealing ring are respectively abutted against the cover glass and the glass slide when the cover glass, the intermediate glass slide and the glass slide are sequentially attached.
2. A slide structure suitable for use in a confocal microscope according to claim 1, wherein: a first gap is arranged between the inner side wall of the first through hole and the sealing ring.
3. A slide structure suitable for use in a confocal microscope according to claim 1, wherein: the color of the sealing ring is black.
4. A slide structure suitable for use in a confocal microscope according to claim 1, wherein: the number of the first through holes is two.
5. A slide structure suitable for use in a confocal microscope according to claim 1, wherein: the clamping piece is provided with an upper clamping part and a lower clamping part, the cover glass, the middle glass sheet and the glass slide are arranged between the upper clamping part and the lower clamping part, the upper clamping part is abutted against the cover glass, and the lower clamping part is abutted against the glass slide, so that the cover glass, the middle glass sheet and the glass slide are sequentially attached to one another.
6. The slide structure for confocal microscopy according to claim 5, wherein: the cover glass is in a transversely arranged rectangular plate shape, the number of the clamping pieces is two, and the two clamping pieces are respectively arranged at the left end and the right end of the cover glass.
7. The slide structure for confocal microscopy according to claim 5, wherein: the clamping piece is a clip.
Technical Field
The invention relates to the field of experimental equipment, in particular to a glass slide structure suitable for a confocal microscope.
Background
The laser confocal microscope used in many laboratories in China at present is of an inverted type, and generally, a mixture of an observed object, a transparent agent and a fluorescent dye is placed above an objective lens in two ways; one of the modes is to use a confocal special culture dish, a glass sheet with the thickness of about 0.17mm is embedded at the bottom of a plastic culture dish body, the glass sheet is bonded with the bottom of the culture dish body through a special transparent biological adhesive, and the confocal special culture dish is directly placed above an objective lens, but the confocal special culture dish is difficult to clean and sterilize and can only be used once, so that the experiment cost is high; the other mode is that a conventional combined structure of a cover glass and a glass slide is used, an observed object is placed on the glass slide, a transparent agent and a fluorescent dye are dripped, then the glass slide is covered, then the whole combined structure of the cover glass and the glass slide is turned over and then placed above an objective lens, due to the surface tension effect of liquid, the cover glass and the glass slide can be kept attached and cannot fall off, but the space between the glass slide and the glass slide is small, the transparent agent and the fluorescent dye are easy to overflow, most of the transparent agent (such as wintergreen oil) and the fluorescent Dye (DAPI) used in an experiment have pungent smell and toxicity, and the common contact can cause harm to a human body.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art. Therefore, the invention provides a glass slide structure suitable for a confocal microscope, and a transparent agent and a fluorescent dye are not easy to overflow when the glass slide structure is used.
A slide structure suitable for a confocal microscope according to an embodiment of the present invention includes: the middle glass slide is provided with a plurality of first through holes; the cover glass, the intermediate glass and the glass slide are sequentially stacked; and the sealing ring is made of elastic material, is arranged in the first through hole, and has a thickness slightly larger than that of the intermediate glass slide, so that the upper side and the lower side of the sealing ring are respectively abutted against the cover glass and the glass slide when the cover glass, the intermediate glass slide and the glass slide are sequentially attached.
The slide structure suitable for the confocal microscope according to the embodiment of the invention has at least the following technical effects: through setting up the sealing washer, put middle slide on the glass slide earlier during the use, then put the sealing washer in first through-hole, then arrange the observation thing in the sealing washer, then cover the glass slide, use external clamping piece clamp cover glass, middle slide and glass slide again, because the upper and lower side of sealing washer respectively with the cover glass slide butt, the sanitizer and fluorochrome are difficult to spill over.
According to some embodiments of the invention, a first gap is provided between an inner side wall of the first through hole and the seal ring.
According to some embodiments of the invention, the seal ring is black in color.
According to some embodiments of the invention, the first through hole is two.
According to some embodiments of the invention, the cover glass, the intermediate glass and the glass slide are arranged between the upper clamping part and the lower clamping part, the upper clamping part is abutted with the cover glass, and the lower clamping part is abutted with the glass slide, so that the cover glass, the intermediate glass and the glass slide are sequentially attached.
According to some embodiments of the invention, the cover glass is in a shape of a transversely arranged rectangular plate, and the number of the clamping members is two, and the two clamping members are respectively arranged at the left end and the right end of the cover glass.
According to some embodiments of the invention, the clamp is a clip.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 is a front elevational view of a slide assembly in accordance with an embodiment of the present invention;
FIG. 2 is a top view of a configuration of a slide assembly of an embodiment of the invention;
figure 3 is a schematic view of the assembled slide assembly and clamping member shown in figure 2.
Reference numerals:
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. The terms "a" or "an" mean one or more, the terms "a" or "an" mean two or more, the terms larger than, smaller than, exceeding, and the like are understood to include the number, and the terms "larger than, smaller than, within, and the like are understood to include the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.