Wireless ultramicro microscopic imaging system based on light stimulation
阅读说明：本技术 基于光刺激的无线超微型显微成像系统 (Wireless ultramicro microscopic imaging system based on light stimulation ) 是由 李卫东 吴正平 魏欢 杨翔宇 于 2019-10-14 设计创作，主要内容包括：本发明公开了一种基于光刺激的无线超微型显微成像系统,涉及显微成像技术领域,解决了现有成像系统成本高、体积大的技术问题,其技术方案要点是该显微成像系统包括无线供电系统、CMOS图像传感器和镜体,无线供电系统省去了有线方式中较为昂贵的换向装置,同时用于传输光刺激命令；CMOS图像传感器采用高集成度的模数转换芯片进行信号采集,电路采用多层板设计,使得设备整体设计体积减小；镜体包括刺激光源,可以实现光刺激。本发明的系统体积小,成本低,使用便捷,能够实时记录荧光图像,实现高精准采集,且传输速度快且稳定,还可以任意光刺激模式。(The invention discloses a wireless ultra-miniature microscopic imaging system based on light stimulation, which relates to the technical field of microscopic imaging and solves the technical problems of high cost and large volume of the existing imaging system; the CMOS image sensor adopts an analog-to-digital conversion chip with high integration level to acquire signals, and a circuit adopts a multilayer board design, so that the overall design volume of the equipment is reduced; the lens body comprises a stimulation light source, and light stimulation can be realized. The system has the advantages of small volume, low cost, convenient use, high transmission speed and stability, can record fluorescence images in real time, realizes high-precision acquisition and can realize any light stimulation mode.)
1. A wireless ultra-miniature microscopic imaging system based on light stimulation is characterized by comprising a wireless power supply system, a CMOS image sensor and a mirror body (100), wherein the CMOS image sensor is connected with the top of the mirror body (100), the mirror body (100) comprises a vertical rod (15) and a horizontal rod (16) which are vertically arranged, an achromatic lens (3) is arranged on the vertical rod (15), focal length adjusting modules (2) are symmetrically arranged on two sides of the achromatic lens (3), an emission filter (5), a first spectroscope (6) and a self-focusing lens (11) are arranged below the achromatic lens (3) from top to bottom, an excitation filter (10), a second spectroscope (9), a hemispherical lens (8) and an excitation light source (7) are arranged on the horizontal rod (16) on the left side of the first spectroscope (6) from left to right, a stimulation light source (4) is arranged above the second spectroscope (9), the self-focusing lens (11) is arranged at the bottom of the lens body (100).
2. The photostimulation-based wireless subminiature microscopy imaging system according to claim 1, wherein the emission filter (5) and the excitation filter (10) are arranged vertically.
3. The photostimulation-based wireless subminiature microscopy system according to claim 2, wherein the first beam splitter (6) and the second beam splitter (9) are disposed at an acute angle to the x-axis.
4. The photostimulation-based wireless subminiature microscopy imaging system according to any one of claims 1-3, wherein the imaging system further comprises a data processing module and a data analysis module, the data processing module is connected to the CMOS image sensor, and the data analysis module is connected to the data processing module.
5. The wireless ultra-miniature microscopic imaging system based on light stimulation according to claim 4, wherein said data processing module is ATMEL SAM V71 microprocessor, and said data processing module is wirelessly connected with said CMOS image sensor.
6. The photostimulation-based wireless subminiature microscopy imaging system according to claim 5, further comprising a scope applicator disposed above the scope, a head fixation plate disposed below the scope, and a scope protector disposed below the head fixation plate.
7. The wireless ultra-miniature microscopic imaging system based on light stimulation according to claim 6, wherein said wireless power supply system comprises a wireless power supply module, a wireless receiving module, a light lithium battery, a power supply voltage stabilizer and a fuel gauge.
Technical Field
The present disclosure relates to a microscopic imaging system, and more particularly, to a wireless ultra-miniature microscopic imaging system based on optical stimulation.
Background
The traditional optical imaging system has a complex structure, a large volume and high price, and is difficult to realize the research on brain nerve activity imaging and the like during free activity. Microscopic imaging techniques are widely used in cell biology, neuroscience and other research, and are important means for neurobiology and medical research.
The fluorescence imaging system structure in the existing scientific research is generally composed of an optical path and a circuit, wherein the optical path generally comprises an ocular lens, an objective lens, an optical fiber, an optical filter, an achromatic lens, a dichroic mirror, a condensing lens and a focal length adjusting structure unit, and the circuit comprises a power management unit, an excitation light unit, a CMOS imaging power supply and a data conversion and transmission unit. The structures are various in composition, and are difficult to miniaturize, so that the structure is suitable for various scientific researches under the condition of free movement.
When large-scale equipment such as functional nuclear magnetism, PET, CT and the like is used for imaging cerebral neurons of moving animals, fixation and constraint are also needed, under the condition, the cerebral nerve activity cannot be completely and really reflected, and a lot of behavioral studies cannot be researched. Therefore, with the rapid development of fluorescent molecular labeling technology, the research of light and freely movable microscopy technology in the aspects of researching neural circuits, cell activities and the like is urgent.
In addition, the existing imaging equipment does not have the function of simultaneously performing optical stimulation, and the optical stimulation equipment needs to be separately provided in the research process, so that a lot of constraints and inconvenience are brought to experiments and researches.
Disclosure of Invention
The technical purpose of the disclosure is to overcome the defects of the prior art, provide a wireless ultramicro microscopic imaging system based on light stimulation with low cost and small volume, provide a new device installation, data recording and light stimulation mode, and solve the problem that the solution of wired transmission in the prior device limits the free movement of a tested object.
The technical purpose of the present disclosure is achieved by the following technical solutions:
the utility model provides a wireless ultramicro microscopic imaging system based on light stimulation, includes power supply system, CMOS image sensor and the mirror body, CMOS image sensor with the mirror body top is connected, the mirror body is including vertical pole and the horizon bar of perpendicular setting, be equipped with achromatic lens on the vertical pole, achromatic lens bilateral symmetry is equipped with focus adjusting module, achromatic lens below top-down is equipped with emission filter, first spectroscope and self-focusing lens, first spectroscope is left be equipped with excitation filter, second spectroscope, hemisphere lens and excitation light source from left to right on the horizon bar, second spectroscope top is equipped with amazing light source, self-focusing lens are established the bottom of mirror body.
Further, the emission filter and the excitation filter are vertically disposed.
Further, the first beam splitter and the second beam splitter are disposed at an acute angle to the x-axis.
Furthermore, the imaging system further comprises a data processing module and a data analysis module, wherein the data processing module is connected with the CMOS image sensor, and the data analysis module is connected with the data processing module.
Further, the data processing module is an ATMEL SAM V71 microprocessor, and the data processing module is connected with the CMOS image sensor in a wireless manner.
Further, imaging system still includes the mirror body adds holder, head fixed plate and mirror body protector, the mirror body adds the holder and establishes mirror body top, the head fixed plate is established mirror body below, the mirror body protector is established head fixed plate below.
Furthermore, the power supply system is a wireless power supply system, and the wireless power supply system comprises a wireless power supply module, a wireless receiving module, a light lithium battery, a power supply voltage stabilizer and a fuel gauge.
The beneficial effect of this disclosure lies in: the system disclosed by the invention has the advantages of small volume, low cost, convenience in use, capability of recording fluorescence images in real time, high-precision acquisition, high and stable transmission speed and capability of realizing any light stimulation mode;
the CMOS image sensor adopts an analog-to-digital conversion chip with high integration level to acquire signals, and a circuit adopts a multilayer board design, so that the overall design volume of the equipment is reduced, and the weight is reduced by about 3 g;
the wireless power supply mode can save the need of using a reversing device to unscrew the signal wire in a wired mode, the reversing device is expensive, the wireless mode can save the expenditure of the part, and the wireless power supply mode is used for transmitting the optical stimulation command;
the device is provided with a mechanical focal length adjusting module, and can realize focal length adjustment within a certain range.
Drawings
FIG. 1 is a schematic view of a mirror structure according to the present disclosure;
FIG. 2 is a schematic view of the mirror mount;
FIG. 3 is a schematic diagram of a system synchronization application;
in the figure: 100-a mirror body; 1-a CMOS image sensor; 2-a focal length adjustment module; 3-an achromatic lens; 4-a stimulus light source; 5-an emission filter; 6-a first beam splitter; 7-an excitation light source; 8-hemispherical lens; 9-a second beam splitter; 10-an excitation filter; 11-a self-focusing lens; 12-a lens holder; 13-head fixation plate; 14-a scope protector; 15-vertical rod; 16-horizontal bar.
Detailed Description
The technical scheme of the disclosure will be described in detail with reference to the accompanying drawings.
In the description of the present disclosure, it is to be understood that the terms "first", "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit indicating the number of technical features indicated, whereby the features defined "first", "second" may explicitly or implicitly include at least one such feature. In addition, unless expressly stated or limited otherwise, the term "coupled" is intended to be inclusive, e.g., that may be fixedly coupled, removably coupled, or integrally coupled; can be mechanically or electrically connected; the connection may be direct or indirect via an intermediate medium, and may be a communication between the two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those of ordinary skill in the art.
Likewise, the terms "top," "bottom," "above," "below," "vertical," "horizontal," "two sides," "left side," "top-down," "left-to-right," and the like, refer to an orientation or positional relationship illustrated in the drawings for convenience in describing and simplifying the disclosure, and do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the disclosure.
The wireless ultramicro microscopic imaging system based on optical stimulation comprises a wireless power supply system, a CMOS image sensor 1 and a
The wireless power supply system comprises a wireless power supply module, a wireless receiving module, a light lithium battery, a power supply voltage stabilizer and a fuel gauge. The wireless power supply system is realized by using an electromagnetic induction technology, wherein a power transmission coil of a wireless power supply module generates a changing magnetic field for an alternating current signal with a certain frequency, and a power receiving coil in a wireless receiving module generates current voltage stabilization after being converted by the magnetic field to charge a battery or supply power to the system. The power supply voltage stabilizer selects a TPS62170 chip of the TI, outputs 3.3V serving as working voltage to be supplied to each part; the electricity meter selects an LT LTC2941 chip to monitor the battery electricity quantity in real time and can give an early warning when the electricity quantity is too low. The wireless power supply system is connected with the mirror body and the CMOS image sensor in a wireless mode.
The working process of the
The stimulating light source 4 is reflected by the second spectroscope 9, clutter is filtered by the exciting light filter 10, the stimulating light source 4 is reflected to the self-focusing lens 11 by the first spectroscope 6 and enters the brain of the tested animal, and the stimulating light source 4 is responsible for adjusting the light environment in the brain and can not cause calcium ion transition and imaging different from the exciting light source 7.
The imaging system further comprises a data processing module, a data analysis module, a
Preferably, the CMOS image sensor 1 is a CMOS image sensor MT9V032 of an ON Semiconductor, the pixel is 752(H) × 480(V), the frame rate can reach 60fps, and the data transmission can be performed by using a 10-bit parallel data port or 8-bit or 10-bit LVDS serial transmission.
Preferably, the data processing module selects ATMEL SAM V71 microprocessor, the dominant frequency is up to 300MHz, possess multiple serial interfaces, take charge of communicating with each part, the microprocessor integrates and has a floating point arithmetic unit, can carry on some simple processing algorithms; the CMOS image sensor has a COMS camera parallel interface, and can perform data interaction with the CMOS image sensor; the storage device is provided with an SDIO interface, data can be conveniently and rapidly stored in the storage device, a storage part adopts a TF memory card with high speed, high capacity and small volume, the reading and writing speed is Class10, the storage space is 64GB, and the data storage of a whole day is guaranteed. The synchronous signal processing part also adopts a wireless scheme for communication, and a user records the synchronous signals into a signal sequence acquired in real time in a wireless mode, synchronizes the state of data and can also realize low-frame-rate preview of the signals.
Preferably, the NORDIC nRF24 chip is selected in a wireless mode, the NORDIC nRF24 chip works in a 2.4GHz frequency band with ultra-low power consumption, the networking expansion function is achieved, and wireless communication comprises a storage control scheme, an image acquisition parameter setting command protocol and a light stimulation scheme communication protocol.
Preferably, the data analysis module may perform data Image reading, Image Registration (Image Registration), Baseline calculation (Image), automatic classification (automatic classification), Δ F/F Trace Extraction (Δ F/F Trace Extraction), Deconvolution processing (Deconvolution), and the like, and may also perform short-time online preview of images.
As a specific embodiment, the weight of the whole system obtained by the method is about 3 g, and when the system is wirelessly powered, the system is installed on the head of a tested animal or a position to be researched, as shown in figure 3, and is used for image acquisition and storage; when the power is supplied by a battery, the wireless power supply system can be installed in two parts, the mirror body part is fixed on the head of an animal or a position to be researched, the wireless power supply system is installed on the back of a tested animal, and the volume of the wireless power supply system part is mainly determined according to the type of the tested animal, for example, the wireless power supply system installed on a mouse is about 8 grams. After the animal is installed on the animal body, the images collected by the CMOS image sensor can be synchronously transmitted with other equipment in a wireless mode.
In conclusion, the system disclosed by the invention has the advantages of small volume, low cost and convenience in use, can record fluorescence images in real time, has the channel sampling rate of 60fps and the sampling precision of 1 mu m/pixel, realizes high-precision acquisition, is high and stable in transmission speed, and can change the wavelength range of the stimulation light source to realize any light stimulation mode.
The CMOS image sensor and the data processing module select an analog-to-digital conversion chip with high integration level for signal acquisition, and the circuit adopts a multi-layer board design, so that the overall design volume of the equipment is reduced, and the weight is reduced by about 3 g.
By adopting the wireless power supply system, a reversing device in a wired mode can be omitted to unscrew the signal wire. The reversing device is expensive, the expenditure of the reversing device can be saved in a wireless mode, and the reversing device is used for transmitting the light stimulation command.
The mechanical focal length adjusting module is provided, so that the focal length adjustment within a certain range is realized.
The system is arranged on the body of the tested animal,mirror bodyThe weight of the battery power supply part in the system is 5 g at least, and the battery power supply part is arranged on the back of the tested animal, so that the free movement of the tested animal can be allowed to the maximum extent.
The foregoing is an exemplary embodiment of the present disclosure, and the scope of the present disclosure is defined by the claims and their equivalents.
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