阅读说明：本技术 一种农用杀菌组合物 (Agricultural bactericidal composition ) 是由 朱刚 于 2019-10-24 设计创作，主要内容包括：本发明涉及一种农用杀菌组合物,有效成分由噻菌灵、戊唑醇、肟菌酯组成,噻菌灵、戊唑醇、肟菌酯在杀菌组合物中的重量比例分别为1-10：1-10：1-10；本发明杀菌组合物用于水稻种子处理,可有效提高播种后种子的出苗率,对苗期病虫害具有很好的预防作用。(The invention relates to an agricultural sterilization composition, the effective components of which comprise probenazole, tebuconazole and trifloxystrobin, the weight proportions of the probenazole, the tebuconazole and the trifloxystrobin in the sterilization composition are respectively 1-10: 1-10: 1-10; the bactericidal composition is used for treating rice seeds, can effectively improve the emergence rate of the sowed seeds, and has a good prevention effect on plant diseases and insect pests in the seedling stage.)

1. An agricultural bactericidal composition, which is characterized in that: the effective components comprise probenazole, tebuconazole and trifloxystrobin, and the weight ratio of the probenazole, the tebuconazole and the trifloxystrobin the bactericidal composition is 1-10: 1-10: 1-10.

2. The agricultural bactericidal composition as set forth in claim 1, wherein: the weight ratio of probenazole, tebuconazole and trifloxystrobin in the bactericidal composition is 5:1: 3.

3. The agricultural bactericidal composition as set forth in claim 1, wherein: the weight ratio of probenazole, tebuconazole and trifloxystrobin in the bactericidal composition is 1:1: 10.

4. The agricultural bactericidal composition as set forth in claim 1, wherein: the weight percentage of the probenazole in the composition is 1-20 percent; the weight percentage of the tebuconazole in the composition is 1-20 percent; the weight percentage of the trifloxystrobin in the composition is 1-20%.

5. The agricultural bactericidal composition as set forth in claim 1, wherein: the weight percentage of the probenazole in the composition is 8 percent; the weight percentage of the tebuconazole in the composition is 2 percent; the weight percentage of the trifloxystrobin in the composition is 2%.

6. An agricultural sterilization composition is characterized by comprising 8% of thiabendazole, 2% of tebuconazole, 2% of trifloxystrobin, 2% of dodecylbenzene sulfonate, 0.4% of PVA, 2% of brilliant red, 1% of glycol, 0.2% of preservative, 2% of film-forming agent, 0.1% of defoaming agent and the balance of distilled water.

7. The use of the agricultural bactericidal composition of claim 1 for rice seed treatment to control rice seedling diseases.

Technical Field

The invention relates to the field of pesticide bactericides, and the active ingredients of the bactericide comprise probenazole, tebuconazole and trifloxystrobin and are used for treating rice seeds to prevent and control rice seedling diseases.

Background

In modern agricultural production, the seed is treated with seed coating agent, and this is one of the economic and simple methods for preventing and controlling pests in seedling stage and soil-borne and seed-borne diseases of crops. Seed coating is a formulation of agricultural chemicals with film-forming properties for the treatment of crop or other plant seeds, which cures on the surface of the seed to form a film called a seed coating. The seed coating agent absorbs water and swells in soil without being dissolved, allows water and air required by normal germination of seeds to pass through, slowly releases substances such as pesticides and seed fertilizers, can kill pests in seedling stage and pests in seedling stage, and has the effects of preventing and treating diseases and systemic diseases in seedling stage, improving the germination rate of seeds, promoting healthy growth of seedlings and the like.

The suspended seed coating agent is one kind of seed coating agent and is one kind of flowable stable homogeneous suspension prepared with pesticide, filming agent, wetting agent, dispersant, thickener, colorant, stuffing and water and through wet crushing. Because of its convenience and safety in use, it is favored among a wide variety of seed coating agents.

Thiabendazole belongs to benzimidazole germicide, has an action mechanism of inhibiting the formation of tubulin in the mitotic process of fungi, has the property of internal absorption to apical conduction, but cannot conduct to basal group. The long-lasting effect is long, and the pesticide has cross resistance with benzimidazole fungicides. Has inhibitory activity on ascomycetes, basidiomycetes and adelomycetes, and can be used for preventing and treating various crop fungal diseases and preventing and preserving fruits and vegetables. Is a high-efficiency, broad-spectrum and internationally universal bactericide.

Tebuconazole, a triazole bactericidal pesticide with high efficiency, broad spectrum and systemic property, has three functions of protection, treatment and eradication, and has wide bactericidal spectrum and long lasting period. Like all triazole fungicides, tebuconazole is able to inhibit the biosynthesis of fungal ergosterol. Tebuconazole is used as a seed treatment agent and foliar spray all over the world, has a wide bactericidal spectrum, and not only has high activity but also has a long lasting period. Tebuconazole is mainly used for preventing and treating various fungal diseases of crops such as wheat, rice, peanuts, vegetables, bananas, apples, pears, corn sorghum and the like, and is registered and widely applied to more than 60 crops in more than 50 countries all over the world. The bactericidal composition is used for preventing and treating sclerotinia rot of colza, has good prevention effect, has the characteristics of lodging resistance, obvious yield increase effect and the like, and has an action mechanism on pathogenic bacteria, namely, the demethylation of ergosterol on cell membranes of the pathogenic bacteria is inhibited, so that the pathogenic bacteria cannot form the cell membranes, and the pathogenic bacteria are killed.

The trifloxystrobin has the properties of broad spectrum, penetration, rapid distribution and the like, is quickly absorbed by crops, has upward systemic property, and thus has good rain erosion resistance and long duration, so the trifloxystrobin is considered as a second-generation methoxy acrylate bactericide, has excellent protective activity and certain therapeutic activity, and the activity is not influenced by the environment.

The invention provides a deep research on the application of probenazole, tebuconazole and trifloxystrobin to seed treatment, and finds that the combination of probenazole, tebuconazole and trifloxystrobin is used for treating rice seeds on the basis of a large number of tests, so that the disease and insect resistance of the seeds is obviously improved, and the healthy growth of plant seeds is improved. The present invention has been completed by further studies.

Disclosure of Invention

The invention aims to solve the problem of rice seedling diseases and provides a bactericidal composition which comprises active ingredients of probenazole, tebuconazole and trifloxystrobin and is used for treating rice seeds to prevent and treat the rice seedling diseases.

In order to realize the purpose of the invention, the invention adopts the following technical scheme: the agricultural bactericidal composition comprises the effective components of probenazole, tebuconazole and trifloxystrobin, wherein the weight ratio of the probenazole, the tebuconazole and the trifloxystrobin in the bactericidal composition is respectively 1-10: 1-10: 1-10.

Further, the weight percentage of the probenazole in the composition is 1-20%; the weight percentage of the tebuconazole in the composition is 1-20 percent; the weight percentage of the trifloxystrobin in the composition is 1-20%.

The bactericidal composition also contains inactive additives, and mainly comprises common pesticide preparation additives such as wetting powder, a film forming agent, a coloring agent, a thickening agent, an antifreezing agent, a preservative, a defoaming agent and the like.

In order to promote wetting dispersion among active component particles and prevent mutual coagulation, the wetting dispersant selected by the invention is preferably one or more than two of fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene, polyoxyethylene fatty acid, alkylaryl polyglycol ether, alkyl sulfonate, aryl sulfonate, fatty acid polyglycol, dodecyl benzene sulfonate, polyoxyethylene phenol formaldehyde condensate, polyoxyethylene polyoxypropylene ether block copolymer, naphthalene or alkyl naphthalene formaldehyde condensate sulfonate, fatty alcohol polyoxyethylene ether sulfonate, alkylphenol polyoxyethylene ether sulfonate, lignin and derivative sulfonate, fatty acid ethane adduct phosphate, alkylphenol polyoxyethylene ether phosphate and alkylphenol polyoxyethylene ether formaldehyde condensate sulfate.

In order to ensure that limited pesticide components act on seeds and roots in a concentrated manner, the pesticide components are not easy to fall off and fly away, and the diffusion of the pesticide in the environment and the contact of the pesticide with non-targets are reduced, the selected film forming agent ensures that the prepared film is good in air permeability and water permeability and the seeds normally germinate and grow, and also ensures that the prepared film is difficult to dissolve in water, active components in the film can be slowly released, and the effect duration is long. The film forming agent is preferably one or more of acacia, animal glue, pectin, xanthan gum, methyl cellulose, ethyl cellulose, sodium carboxymethyl cellulose, hydroxypropyl cellulose, sodium alginate, polyvinyl alcohol, polyethylene glycol, polyvinylpyrrolidone, polyacrylamide and sodium polyacrylate.

The coloring agent is also called as policeman agent, which can improve the use safety of the suspended seed coating agent, wherein the coloring agent selected by the invention is preferably one or more than two of alkaline rose essence, aqueous rose bengal and acid scarlet.

The inactive additive of the present invention further includes a thickener to increase the viscosity of the dispersion medium and reduce the settling rate of the particles, thereby improving the stability of the formulation. Preferably, the tackifier is one or more than two of xanthan gum, Arabic gum, methylcellulose, carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, sodium acrylate, sodium alginate, polyvinyl alcohol, polyvinylpyrrolidone, polyacrylamide, sodium polyacrylate, magnesium aluminum silicate and bentonite.

In order to improve the low-temperature storage stability of the seed coating agent, the invention also adds an antifreezing agent which is one or more than two of ethylene glycol, propylene glycol, glycerol, hexanediol, urea, ammonium sulfate, sodium chloride and calcium chloride.

The defoaming agent is organic silicone and C_8-10Fatty alcohol of (2), C_10-20One or more of the saturated fatty acids and esters thereof; the preservative is benzoic acid, benzaldehyde, sodium benzoate, potassium benzoate, sorbic acid, sodium benzoate, potassium sorbate, sodium benzoate, potassium benzoate,One or more than two of sodium salicylate, 2-hydroxy biphenyl and p-hydroxybenzaldehyde 1, 2-benzothiazoline-3-ketone.

The bactericidal composition can be processed into a suspended seed coating agent in practical application, and the preparation method comprises the following steps: airflow crushing probenazole, tebuconazole and trifloxystrobin, wetting dispersant, film forming agent, coloring agent, thickening agent, antifreezing agent, preservative, defoaming agent and distilled water, and grinding for 2 times in a sand mill according to a proportion until the particle fineness of the suspension reaches D₅₀Is 2-3 μm, D₉₀Less than 8 μm to obtain the product. In the actual application process, the invention can determine the appropriate dosage of the suspended seed coating according to the occurrence condition of local control objects, and the application dosage is that the mass ratio of the seed coating to seeds is 1: 200-600.

The suspension seed coating agent prepared by the invention is bright red flowable suspension, the low-temperature and heat storage stability is qualified, the heat storage decomposition rate is less than 5 percent, the suspension rate is more than 90 percent, 98 percent of the wet sieve test passes through a standard sieve with the particle size of 45 mu m, and the particle fineness D is₅₀Is 2-3 μm.

The combination of active ingredients of different seed coating agents is usually expressed as synergy, additive action or antagonism due to the difference of composition, structure and physical and chemical properties of the active ingredients. The synergistic effect is the mutual cooperation between different substances, and the generated effect is larger than the sum of the effects of the components; and additive action is that it produces an effect equal to the sum of the effects of the individual components; antagonism is a phenomenon in which when two substances act on a living organism, one substance interferes with the effect of the other substance or interferes with each other, so that the overall effect is reduced.

The detection and analysis method of the indoor bactericidal activity comprises the following steps:

adopting a medicine-containing culture medium method: 6mL of the liquid medicine with the series concentration of single agent and mixed agent is taken and added into 54mL of PDA culture medium cooled to 45 ℃ to prepare a medicine-containing culture medium plate with the required final concentration. Then, 6mm diameter hypha blocks were prepared from the edges of the colonies of the rice bakanae disease bacteria cultured for 7 days, and transferred to each series of drug-containing culture media with the hypha facing downward, and each treatment was repeated 4 times. After the treatment, the mixture is placed in a constant temperature biochemical incubator at 25 +/-1 ℃ for culture.

The data analysis method adopts the following steps: the synergy of the mixed medicament is evaluated according to a co-toxicity coefficient method (CTC) of Sun & Johnson (1960), namely that the CTC is less than or equal to 80 and is antagonistic, the CTC is more than 80 and is less than 120 and is additive, and the CTC is more than or equal to 120 and is synergistic.

Table 1 shows the toxicity test results of the blending of probenazole, tebuconazole and trifloxystrobin on bakanae disease germs of rice.

	EC50(μg/mL)	Actual ATI	Theoretical ATI	Co-toxicity coefficient
					Thiabendazole	6.38	100	--	--
Tebuconazole	1.21	527.27	--	--
					Trifloxystrobin	2.19	291.32	--	--
Thiabendazole: tebuconazole trifloxystrobin (1:10:1)	1.01	631.68	472.00	133.83
					Thiabendazole: tebuconazole trifloxystrobin (1:5:3)	1.17	545.30	401.15	135.93
Thiabendazole: tebuconazole trifloxystrobin (1:3:5)	1.26	506.35	348.72	145.20
					Thiabendazole: tebuconazole trifloxystrobin (1:1:10)	1.31	487.02	295.04	165.07
Thiabendazole: tebuconazole trifloxystrobin (3:1:5)	1.65	386.67	253.77	152.37
					Thiabendazole: tebuconazole trifloxystrobin (5:1:3)	1.82	350.55	211.25	165.94
Thiabendazole: tebuconazole trifloxystrobin (10:1:1)	2.98	214.09	151.55	141.27

According to the technical scheme, the probenazole, the tebuconazole and the trifloxystrobin are compounded to serve as effective components, and the probenazole, the tebuconazole and the trifloxystrobin are mixed according to the ratio of 1-10: 1-10:1-10 has the common toxicity coefficient higher than 120 for the rice bakanae disease bacteria, and has obvious synergistic effect.

Detailed Description

The embodiment of the invention discloses a suspended seed coating agent. Those skilled in the art can modify the process parameters appropriately to achieve the desired results with reference to the disclosure herein. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the invention has been described in terms of embodiments, it will be apparent to those skilled in the art that the technology can be practiced and applied by modifying or appropriately combining the embodiments described herein without departing from the spirit and scope of the invention.

For further understanding of the present invention, a suspension seed coating agent provided by the present invention will be described in detail with reference to the following examples.