阅读说明：本技术 一种微型组装式层状生物传感器结构 (Miniature assembled layered biosensor structure ) 是由 李冠华 董青龙 ***华 颜丹 于 2019-10-16 设计创作，主要内容包括：本发明提供了一种微型组装式层状生物传感器结构,包括第一柔性电路板和第二柔性电路板,第一柔性电路板和第二柔性电路板折弯成弓形,第一柔性电路板和第二柔性电路板层状分布；第一柔性电路板位于第二柔性电路板的内侧；第一柔性电路板的第一折弯臂向外伸出第一电极,第二柔性电路板的第一折弯臂向内伸出第二电极；第一电极和第二电极一个为工作电极,另一个为对电极；第一电极和第二电极构成一个传感器单元；将工作电极WE、对电极CE/参比电极RE分离,可以分别实现批量加工；同时在工作电极涂覆酶和固定酶的时候,因为工作电极WE和其他电极离的很远,可以很方便的进行精细化操作；提高了生产效率和设计精度。(The invention provides a miniature assembled layered biosensor structure, which comprises a first flexible circuit board and a second flexible circuit board, wherein the first flexible circuit board and the second flexible circuit board are bent into an arc shape and are distributed in a layered manner; the first flexible circuit board is positioned at the inner side of the second flexible circuit board; the first bending arm of the first flexible circuit board extends outwards to form a first electrode, and the first bending arm of the second flexible circuit board extends inwards to form a second electrode; one of the first electrode and the second electrode is a working electrode, and the other electrode is a counter electrode; the first electrode and the second electrode constitute a sensor unit; the working electrode WE and the counter electrode CE/reference electrode RE are separated, so that batch processing can be realized respectively; meanwhile, when the working electrode is coated with enzyme and fixed with enzyme, because the working electrode WE is far away from other electrodes, the fine operation can be conveniently carried out; the production efficiency and the design precision are improved.)

1. The structure of the miniature assembled layered biosensor is characterized by comprising a first flexible circuit board (1) and a second flexible circuit board (2), wherein the first flexible circuit board (1) and the second flexible circuit board (2) are bent into a bow shape, and the first flexible circuit board (1) and the second flexible circuit board (2) are distributed in a layered manner; after being bent into an arc shape, the first flexible circuit board (1) is positioned at the inner side of the second flexible circuit board (2);

the first bending arm of the first flexible circuit board (1) extends outwards to form a first electrode (11), and the first bending arm of the second flexible circuit board (2) extends inwards to form a second electrode (21);

one of the first electrode (11) and the second electrode (21) is a working electrode, and the other one is a counter electrode; the first electrode (11) and the second electrode (21) form a sensor unit (3).

2. The micropackaged layered biosensor structure of claim 1, wherein the top surface of the first electrode (11) is opposite to the top surface of the second electrode (21), and the reaction membranes of the first electrode (11) and the second electrode (21) are located on the top surfaces; a first micro flow channel (41) is formed between the top surfaces of the first electrode (11) and the second electrode (21).

3. The structure of the micro-assembled layered biosensor according to claim 2, wherein the number of the first electrodes (11) is N, N first electrodes (11) form a column, and adjacent first electrodes (11) are connected by a first wall pillar (51);

the number of the second electrodes (21) is N, the N second electrodes (21) form a row, and the adjacent second electrodes (21) are connected through second wall columns (52);

the first electrode (11), the first wall column (51), the second electrode (21) and the second wall column (52) jointly form the first micro-channel (41);

n is a natural number, and N is more than or equal to 2.

4. The micro-fabricated layered biosensor structure according to claim 3, further comprising a housing (6), wherein the housing (6) is internally provided with a first upper side card slot (61) and a first lower side card slot (62);

the first upper side card slot (61) is divided into a first left side upper card slot (611) and a first right side upper card slot (612), and a position between the first left side upper card slot (611) and the first right side upper card slot (612) is vacant;

the first lower clamping groove (62) is divided into a first left lower clamping groove (621) and a first right lower clamping groove (622), and a position between the first left lower clamping groove (621) and the first right lower clamping groove (622) is vacant;

the first electrode (11) is located in the middle of the first flexible circuit board (1) in the width direction, and the edges of the first flexible circuit board (1) on the two sides of the first electrode (11) are respectively clamped into the first left upper clamping groove (611) and the first right upper clamping groove (612);

the edge parts of two sides of the second bending arm of the first flexible circuit board (1) are respectively clamped into the first left lower clamping groove (621) and the first right lower clamping groove (622);

the inner side wall of the shell (6), the first electrode (11), the first wall column (51), the second electrode (21) and the second wall column (52) jointly form the first micro-channel (41).

5. The micro-fabricated layered biosensor structure according to claim 4, wherein the housing (6) is internally provided with a second upper side card slot (63) and a second lower side card slot (64);

the second upper side clamping groove (63) is divided into a second left side upper clamping groove (631) and a second right side upper clamping groove (632), and a gap is reserved between the second left side upper clamping groove (631) and the second right side upper clamping groove (632);

the second lower clamping groove (64) is divided into a second left lower clamping groove (641) and a second right lower clamping groove (642), and a gap is reserved between the second left lower clamping groove (641) and the second right lower clamping groove (642);

the second electrode (21) is located in the middle of the second flexible circuit board (2) in the width direction, and the side portions of the second flexible circuit board (2) on the two sides of the second electrode (21) are respectively clamped into the second left upper clamping groove (631) and the second right upper clamping groove (632);

and the edge parts of two sides of a second bending arm of the second flexible circuit board (2) are respectively clamped into the second left lower clamping groove (641) and the second right lower clamping groove (642).

6. The micropackaged layered biosensor structure of claim 5, wherein the housing (6) has a rear end that extends beyond the support arm (8);

the first bending arm and the second bending arm of the first flexible circuit board (1) are connected through a bending part (9), the first bending arm and the second bending arm of the second flexible circuit board (2) are connected through the bending part (9), and the bending part comprises more than 4 lines;

the bending part (9) is attached to the tail end of the supporting arm (8), and two surfaces of the inner side of the bending part of the first flexible circuit board (1) are respectively attached to the upper wall surface and the lower wall surface of the supporting arm (8).

7. The structure of the micro-assembled layered biosensor according to claim 5, further comprising a third flexible circuit board (7), wherein the third flexible circuit board (7) is bent into an arc shape, and the third flexible circuit board (7) and the second flexible circuit board (2) are layered; the third flexible circuit board (7) is positioned at the outer side of the second flexible circuit board (2);

the first bending arm of the third flexible circuit board (7) extends inwards to form a third electrode (71), and the first bending arm of the second flexible circuit board (2) extends outwards to form a fourth electrode (22);

one of the third electrode (71) and the fourth electrode (22) is a working electrode, and the other one is a counter electrode; the third electrode (71) and the fourth electrode (22) form a further sensor unit (3).

8. The micropackaged layered biosensor structure of claim 1, wherein the first electrode (11) has a side facing the second electrode (21) and the reactive films of the first (11) and second (21) electrodes are located on the sides; a second microchannel (42) is formed between the first electrode (11) and the side surface of the second electrode (21).

9. The structure of the micro-assembled layered biosensor according to claim 8, wherein the number of the first electrodes (11) is N, N first electrodes (11) form a column, and adjacent first electrodes (11) are connected by a third wall pillar (53);

the number of the second electrodes (21) is N, the N second electrodes (21) form a row, and the adjacent second electrodes (21) are connected through a fourth wall column (54);

the first electrode (11), the third wall column (53), the second electrode (21) and the fourth wall column (54) jointly form the second micro-channel (42);

n is a natural number, and N is more than or equal to 2.

10. The structure of the micro-assembled layered biosensor according to claim 9, wherein a first clamping head (01) is disposed at an end of the first electrode (11), a first clamping blind hole (24) is disposed at an inner side of the second flexible circuit board (2), and the first clamping head (01) is clamped into the first clamping blind hole (24);

the tip of second electrode (21) is provided with second dop (02), the outside of first flexible circuit board (1) sets up second joint blind hole (14), second dop (02) card is gone into second joint blind hole (14).

11. The micropackaged layered biosensor structure of any one of claims 1 to 10, wherein the first flexible circuit board (1) and/or the second flexible circuit board (2) is replaced by a rigid-flex circuit board.

Technical Field

The invention relates to the technical field of biochemical parameter acquisition, in particular to a miniature assembled layered biosensor structure.

Background

Due to the requirement of the working principle, the working electrode and the counter electrode of the biosensor need to be arranged at positions relatively close to each other. In the prior art, in the production process, the process is complex and the manufacturing difficulty is high due to the influence of large material difference between a working electrode and a counter electrode; the manufacturing process requires a large distance between the working electrode and the counter electrode; the contradiction between the manufacturing process and the structural principle limits the development of biosensors.

Disclosure of Invention

In view of the above, the present invention provides a micro-assembly type layered biosensor structure, in which the working electrode and the counter electrode are far apart and are close to each other only after assembly.

A miniature assembled layered biosensor structure comprises a first flexible circuit board and a second flexible circuit board, wherein the first flexible circuit board and the second flexible circuit board are bent into an arch shape and are distributed in a layered manner; the first flexible circuit board is positioned at the inner side of the second flexible circuit board;

the first bending arm of the first flexible circuit board extends outwards to form a first electrode, and the first bending arm of the second flexible circuit board extends inwards to form a second electrode;

one of the first electrode and the second electrode is a working electrode, and the other electrode is a counter electrode; the first electrode and the second electrode constitute one sensor unit.

Preferably, the top surface of the first electrode faces the top surface of the second electrode, and the reaction films of the first electrode and the second electrode are both positioned on the top surfaces; a first microchannel is formed between the top surfaces of the first and second electrodes.

Preferably, the number of the first electrodes is N, the N first electrodes form a row, and adjacent first electrodes are connected through a first wall column;

the number of the second electrodes is N, the N second electrodes form a row, and adjacent second electrodes are connected through a second wall column;

the first electrode, the first wall column, the second electrode and the second wall column jointly form a first micro-channel;

n is a natural number, and N is more than or equal to 2.

Preferably, the device also comprises a shell, wherein a first upper side clamping groove and a first lower side clamping groove are arranged in the shell;

the first upper side clamping groove is divided into a first left side upper clamping groove and a first right side upper clamping groove, and a gap is reserved between the first left side upper clamping groove and the first right side upper clamping groove;

the first lower clamping groove is divided into a first left lower clamping groove and a first right lower clamping groove, and a gap is reserved between the first left lower clamping groove and the first right lower clamping groove;

the first electrode is positioned in the middle of the first flexible circuit board in the width direction, and the edges of the first flexible circuit board on two sides of the first electrode are respectively clamped into the first left upper clamping groove and the first right upper clamping groove;

the side parts of two sides of the second bending arm of the first flexible circuit board are respectively clamped into the first left lower clamping groove and the first right lower clamping groove;

the inner side wall of the shell, the first electrode, the first wall column, the second electrode and the second wall column jointly form a first micro-channel.

Preferably, a second upper side clamping groove and a second lower side clamping groove are formed in the shell;

the second upper side clamping groove is divided into a second left side upper clamping groove and a second right side upper clamping groove, and a gap is reserved between the second left side upper clamping groove and the second right side upper clamping groove;

the second lower side clamping groove is divided into a second left side lower clamping groove and a second right side lower clamping groove, and a gap is reserved between the second left side lower clamping groove and the second right side lower clamping groove;

the second electrode is positioned in the middle of the second flexible circuit board in the width direction, and the edges of the second flexible circuit board on two sides of the second electrode are respectively clamped into the second left upper clamping groove and the second right upper clamping groove;

the edge parts of two sides of a second bending arm of the second flexible circuit board are respectively clamped into a second left lower clamping groove and a second right lower clamping groove.

Preferably, the rear end of the shell extends out of the supporting arm;

the first bending arm and the second bending arm of the first flexible circuit board are connected through a bending part, the first bending arm and the second bending arm of the second flexible circuit board are connected through a bending part, and the bending part comprises more than 4 lines;

the bending part is attached to the tail end of the supporting arm, and two surfaces of the inner side of the bending part of the first flexible circuit board are respectively attached to the upper wall surface and the lower wall surface of the supporting arm.

Preferably, the flexible printed circuit board further comprises a third flexible printed circuit board, wherein the third flexible printed circuit board is bent into an arc shape, and the third flexible printed circuit board and the second flexible printed circuit board are distributed in a layered manner; the third flexible circuit board is positioned at the outer side of the second flexible circuit board;

the first bending arm of the third flexible circuit board extends inwards to form a third electrode, and the first bending arm of the second flexible circuit board extends outwards to form a fourth electrode;

one of the third electrode and the fourth electrode is a working electrode, and the other electrode is a counter electrode; the third electrode and the fourth electrode constitute another sensor cell.

Drawings

The multifunctional adapter housing of the present invention is further described below with reference to the accompanying drawings.

FIG. 1 is a schematic structural diagram of a first flexible circuit board, a second flexible circuit board and a third flexible circuit board according to a first structural embodiment of a micro-assembly layered biosensor in accordance with the present invention.

FIG. 2 is a schematic structural diagram of a first flexible circuit board and a second flexible circuit board of a second embodiment of a micro-assembly layered biosensor structure according to the invention.

FIG. 3 is a schematic structural diagram of a housing and a supporting arm of a second embodiment of a micro-assembly layered biosensor structure according to the present invention.

FIG. 4 is a schematic structural diagram of a first flexible circuit board and a second flexible circuit board of a third embodiment of a micro-assembly layered biosensor structure according to the invention.

FIG. 5 is a schematic structural diagram of a first flexible circuit board and a second flexible circuit board of a fourth embodiment of a micro-assembly layered biosensor structure according to the invention.

FIG. 6 is a schematic view of a partial structure of a supporting screen of a second embodiment of a micro-assembly layered biosensor structure according to the present invention.

In the figure:

1-a first flexible circuit board; 11-a first electrode; 14-a second clamping blind hole; 16-a signal processing module; 2-a second flexible circuit board; 21-a second electrode; 22-a fourth electrode; 24-a first clamping blind hole; 3-a sensor unit; 41-a first microchannel; 42-a second microchannel; 51-a first wall stud; 52-a second wall stud; 53-third wall studs; 54-a fourth wall stud; 55-clamping notches; 6-a shell; 61-a first upper side card slot; 611-a first left upper card slot; 612-first right upper card slot; 62-a first lower card slot; 621-a first left lower card slot; 622-first right lower card slot; 63-a second upper side card slot; 631-a second left upper card slot; 632-second right upper card slot; 64-a second lower side card slot; 641-a second left lower card slot; 642-a second right lower card slot; 65-via holes; 7-a third flexible circuit board; 71-a third electrode; 8-a support arm; 85-clamping notches; 9-bending part; 01-a first chuck; 02-a second chuck; 03-micro-pore canal; 04-supporting the filter screen; 05-transition corner cut.

Example two

Preferably, the side of the first electrode faces the side of the second electrode, and the reaction films of the first electrode and the second electrode are located on the sides; and a second micro-channel is formed between the side surfaces of the first electrode and the second electrode.

Preferably, the number of the first electrodes is N, the N first electrodes form a row, and adjacent first electrodes are connected through a third wall column;

the number of the second electrodes is N, the N second electrodes form a row, and adjacent second electrodes are connected through a fourth wall column;

the first electrode, the third wall column, the second electrode and the fourth wall column jointly form a second micro-channel;

n is a natural number, and N is more than or equal to 2.

Preferably, a first clamping head is arranged at the end part of the first electrode, a first clamping blind hole is arranged on the inner side of the second flexible circuit board, and the first clamping head is clamped into the first clamping blind hole;

the end part of the second electrode is provided with a second clamping head, the outer side of the first flexible circuit board is provided with a second clamping blind hole, and the second clamping head is clamped into the second clamping blind hole.

Preferably, the first flexible circuit board and/or the second flexible circuit board is replaced by a rigid-flex circuit board.

Preferably, the supporting arm extends backwards from the position between the first upper clamping groove and the first lower clamping groove of the inner side wall of the shell;

the supporting arm is provided with a clamping notch, the first flexible circuit board is provided with a signal processing module, and the signal processing module is clamped into the clamping notch.

Preferably, the side wall of the housing is provided with a via hole, and the via hole is located at an inlet of the first micro channel or the second micro channel.

Preferably, the first microchannel and the second microchannel are provided with outlets; and flow guide materials are arranged in the first micro-channel and the second micro-channel.

The invention has the beneficial effects that: the invention provides a miniature assembled layered biosensor structure, which comprises a first flexible circuit board and a second flexible circuit board, wherein the first flexible circuit board and the second flexible circuit board are bent into an arc shape and are distributed in a layered manner; the first flexible circuit board is positioned at the inner side of the second flexible circuit board; the first bending arm of the first flexible circuit board extends outwards to form a first electrode, and the first bending arm of the second flexible circuit board extends inwards to form a second electrode; one of the first electrode and the second electrode is a working electrode, and the other electrode is a counter electrode; the first electrode and the second electrode constitute a sensor unit; the working electrode WE and the counter electrode CE/reference electrode RE are separated, so that batch processing can be realized respectively; meanwhile, when the working electrode is coated with enzyme and fixed with enzyme, because the working electrode WE is far away from other electrodes, the fine operation can be conveniently carried out; the production efficiency and the design precision are improved.