阅读说明：本技术 一种酒泥中白藜芦醇与原花青素的提取方法 (Method for extracting resveratrol and procyanidine from lees ) 是由 纳鹏军 张存智 杨金会 王富强 于 2021-08-03 设计创作，主要内容包括：本发明公开了一种酒泥中白藜芦醇与原花青素的提取方法,其特征在于,包括以下步骤：S1、配制浓度为8-12％的硫酸铵溶液；S2、将乙醇、乙酸乙酯、和S1中制备所得的硫酸铵溶液按照1:2:1比例混合后形成一种提取溶液；S3、将酒泥和S2中制备所得的提取溶液按照料液比1:10(g/ml)的比例加入；S4、将S3制得的溶液在38-42℃下超声混合25-35min后过滤,收集滤液；S5、将S4制得的滤液静置8-12h后,分层,收集上层清液；S6、将S5制得的上层清液用旋转蒸发仪减压蒸馏蒸去溶剂,得到白芦藜醇与原花青素的混合物。本发明的优点在于：萃取率高、操作步骤少、可对白藜芦醇与原花青素同时提取。(The invention discloses a method for extracting resveratrol and procyanidine from lees, which is characterized by comprising the following steps: s1, preparing an ammonium sulfate solution with the concentration of 8-12%; s2, mixing the ethanol, the ethyl acetate and the ammonium sulfate solution prepared in the S1 according to the proportion of 1:2:1 to form an extraction solution; s3, adding the lees and the extraction solution prepared in the S2 according to the ratio of the material to the liquid of 1:10 (g/ml); s4, ultrasonically mixing the solution prepared in the S3 at the temperature of 38-42 ℃ for 25-35min, filtering, and collecting filtrate; s5, standing the filtrate prepared in the S4 for 8-12h, layering, and collecting supernatant; s6, distilling the supernatant liquor obtained in the step S5 by a rotary evaporator under reduced pressure to remove the solvent, and obtaining the mixture of the chenopodium album linn alcohol and the procyanidine. The invention has the advantages that: high extraction rate, few operation steps, and can simultaneously extract resveratrol and procyanidin.)

1. A method for extracting resveratrol and procyanidine from lees is characterized by comprising the following steps:

s1, preparing an ammonium sulfate solution with the concentration of 8-12%;

s2, mixing the ethanol, the ethyl acetate and the ammonium sulfate solution prepared in the S1 according to the proportion of 1:2:1 to form an extraction solution;

s3, adding the lees and the extraction solution prepared in the S2 according to the ratio of the material to the liquid of 1:10 (g/ml);

s4, ultrasonically mixing the solution prepared in the S3 at the temperature of 38-42 ℃ for 25-35min, filtering, and collecting filtrate;

s5, standing the filtrate prepared in the S4 for 8-12h, layering, and collecting supernatant;

s6, distilling the supernatant liquor obtained in the step S5 by a rotary evaporator under reduced pressure to remove the solvent, and obtaining the mixture of the chenopodium album linn alcohol and the procyanidine.

2. The extraction of resveratrol and procyanidins from lees as claimed in claim 1, wherein: the ammonium sulfate solution in S1 was 10%.

3. The extraction of resveratrol and procyanidins from lees as claimed in claim 1, wherein: the ultrasonic mixing time in the S4 is 30min, and the mixing temperature is 40 ℃.

4. The extraction of resveratrol and procyanidins from lees as claimed in claim 1, wherein: the filtrate in S5 was left to stand for 10 h.

Technical Field

The invention relates to the technical field of active substance extraction, in particular to a method for extracting resveratrol and procyanidine from lees.

Background

The lees are precipitate generated in wine production, and contain abundant bioactive substances, such as procyanidin, resveratrol, etc. Procyanidins and resveratrol are of great interest because of their antioxidant, free radical scavenging and cancer cell expansion inhibiting effects. The existing methods all utilize different solvents to extract procyanidine and resveratrol step by step, and have the problems of complicated extraction steps, high toxicity, low yield and the like.

Disclosure of Invention

The invention aims to solve the technical problem of providing a method for extracting resveratrol and procyanidine from wine lees, which has high extraction rate and few operation steps and can simultaneously extract resveratrol and procyanidine.

In order to solve the technical problems, the technical scheme provided by the invention is as follows: a method for extracting resveratrol and procyanidin from lees comprises the following steps:

s1, preparing an ammonium sulfate solution with the concentration of 8-12%;

s2, mixing the ethanol, the ethyl acetate and the ammonium sulfate solution prepared in the S1 according to the proportion of 1:2:1 to form an extraction solution;

s3, adding the lees and the extraction solution prepared in the S2 according to the ratio of the material to the liquid of 1:10 (g/ml);

s4, ultrasonically mixing the solution prepared in the S3 at the temperature of 38-42 ℃ for 25-35min, filtering, and collecting filtrate;

s5, standing the filtrate prepared in the S4 for 8-12h, layering, and collecting supernatant;

s6, distilling the supernatant liquor obtained in the step S5 by a rotary evaporator under reduced pressure to remove the solvent, and obtaining the mixture of the chenopodium album linn alcohol and the procyanidine.

Compared with the prior art, the invention has the advantages that: aiming at the procyanidin and the resveratrol in the lees, a mixed solvent consisting of three solvents, namely ethanol, ethyl acetate and ammonium sulfate solution, is used as an extraction solvent, the two functional components, namely the procyanidin and the resveratrol, are extracted simultaneously at one time, the three solvents, namely the ethanol, the ethyl acetate and the ammonium sulfate solution, are green and environment-friendly solvents, the extraction rate is high, the operation steps are few, and the method is an efficient extraction method.

Further, the ammonium sulfate solution in S1 was 10%.

Further, the ultrasonic mixing time in S4 is 30min, and the temperature during mixing is 40 ℃.

Further, the filtrate in S5 was left standing for 10 h.

Detailed Description

For the purpose of better explaining the present invention, the present invention will be described in detail by way of specific embodiments for easy understanding.

Example one

A method for extracting resveratrol and procyanidin from lees comprises the following steps:

s1, preparing an ammonium sulfate solution with the concentration of 9%;

s2, mixing the ethanol, the ethyl acetate and the ammonium sulfate solution prepared in the S1 according to the proportion of 1:2:1 to form an extraction solution;

s3, adding the lees and the extraction solution prepared in the S2 according to the ratio of the material to the liquid of 1:10 (g/ml);

s4, ultrasonically mixing the solution prepared in the S3 at 42 ℃ for 28min, and then filtering to collect filtrate;

s5, standing the filtrate prepared in the S4 for 10 hours, layering, and collecting supernatant;

s6, distilling the supernatant liquor obtained in the step S5 by a rotary evaporator under reduced pressure to remove the solvent, and obtaining the mixture of the chenopodium album linn alcohol and the procyanidine.

Example two

A method for extracting resveratrol and procyanidin from lees comprises the following steps:

s1, preparing an ammonium sulfate solution with the concentration of 12%;

s2, mixing the ethanol, the ethyl acetate and the ammonium sulfate solution prepared in the S1 according to the proportion of 1:2:1 to form an extraction solution;

s3, adding the lees and the extraction solution prepared in the S2 according to the ratio of the material to the liquid of 1:10 (g/ml);

s4, ultrasonically mixing the solution prepared in the S3 at 42 ℃ for 25-35min, filtering, and collecting filtrate;

s5, standing the filtrate prepared in the S4 for 11 hours, layering, and collecting supernatant;

s6, distilling the supernatant liquor obtained in the step S5 by a rotary evaporator under reduced pressure to remove the solvent, and obtaining the mixture of the chenopodium album linn alcohol and the procyanidine.

EXAMPLE III

A method for extracting resveratrol and procyanidin from lees comprises the following steps:

s1, preparing an ammonium sulfate solution with the concentration of 10%;

s2, mixing the ethanol, the ethyl acetate and the ammonium sulfate solution prepared in the S1 according to the proportion of 1:2:1 to form an extraction solution;

s3, adding the lees and the extraction solution prepared in the S2 according to the ratio of the material to the liquid of 1:10 (g/ml);

s4, ultrasonically mixing the solution prepared in the S3 at 40 ℃ for 30min, and then filtering to collect filtrate;

s5, standing the filtrate prepared in the S4 for 10 hours, layering, and collecting supernatant;

s6, distilling the supernatant liquor obtained in the step S5 by a rotary evaporator under reduced pressure to remove the solvent, and obtaining the mixture of the chenopodium album linn alcohol and the procyanidine.

Although the present invention has been described in detail with reference to the above embodiments, it should be understood by those skilled in the art that: modifications and equivalents may be made thereto without departing from the spirit and scope of the invention and it is intended to cover in the claims the invention as defined in the appended claims.