阅读说明：本技术 一种环保真菌保存液及其制备方法 (Environment-friendly fungus preservation solution and preparation method thereof ) 是由 不公告发明人 于 2019-10-24 设计创作，主要内容包括：本发明提出了一种环保真菌保存液,由以下原料制备而成：中药抗菌水提物、维生素C、稳定剂、固定剂、丙三醇、乙酸和去离子水；所述环保真菌保存液的pH在3.8-4.2之间。本发明通过添加中药抗菌水提物,该组合抗菌中药材得到的水提物具有较好的杀死细菌的效果,具有广谱高效的效果,但对真菌的威力不大,适宜作为真菌的保存液添加物。(The invention provides an environment-friendly fungus preservation solution which is prepared from the following raw materials: traditional Chinese medicine antibacterial water extract, vitamin C, stabilizer, fixative, glycerol, acetic acid and deionized water; the pH value of the environment-friendly fungus preservation solution is between 3.8 and 4.2. According to the invention, by adding the traditional Chinese medicine antibacterial aqueous extract, the aqueous extract obtained by combining the antibacterial traditional Chinese medicines has a good effect of killing bacteria, has a broad-spectrum and efficient effect, has little power on fungi, and is suitable for being used as a preservation solution additive of the fungi.)

1. An environment-friendly fungus preservation solution is characterized by being prepared from the following raw materials: traditional Chinese medicine antibacterial water extract, vitamin C, stabilizer, fixative, glycerol, acetic acid and deionized water; the pH value of the environment-friendly fungus preservation solution is between 3.8 and 4.2.

2. The environment-friendly fungus preserving fluid as claimed in claim 1, which is prepared from the following raw materials in parts by weight: 2-5 parts of traditional Chinese medicine antibacterial water extract, 2-7 parts of vitamin C, 1-3 parts of stabilizer, 1-2 parts of fixative, 10-20 parts of glycerol, a proper amount of acetic acid and 100 parts of deionized water, wherein the acetic acid is used for adjusting the pH value of the preservation solution.

3. The environment-friendly fungus preserving fluid as claimed in claim 2, which is prepared from the following raw materials in parts by weight: 3-4 parts of traditional Chinese medicine antibacterial water extract, 3-6 parts of vitamin C, 2-3 parts of stabilizer, 1.2-1.7 parts of fixative, 12-18 parts of glycerol, a proper amount of acetic acid and 130 parts of deionized water, wherein the acetic acid is used for adjusting the pH value of the preservation solution.

4. The environment-friendly fungus preserving fluid as claimed in claim 3, which is prepared from the following raw materials in parts by weight: 3.2 parts of traditional Chinese medicine antibacterial aqueous extract, 5 parts of vitamin C, 2.4 parts of stabilizing agent, 1.5 parts of fixing agent, 16 parts of glycerol, a proper amount of acetic acid and 125 parts of deionized water, wherein the acetic acid is used for adjusting the pH value of the preservation solution.

5. The environment-friendly fungus preservation solution according to claim 1, wherein the traditional Chinese medicine antibacterial aqueous extract is prepared from the following raw materials: galla chinensis, radix Isatidis, herba Taraxaci, bupleuri radix, hispid Fig, fructus forsythiae, Bulbus Fritillariae Cirrhosae, rhizoma Atractylodis, herba Lagotis and radix Aconiti Kusnezoffii.

6. The environment-friendly fungus preservation solution according to claim 5, wherein the traditional Chinese medicine antibacterial aqueous extract is prepared from the following raw materials in parts by weight: 5-10 parts of gallnut, 10-15 parts of isatis root, 5-15 parts of dandelion, 5-10 parts of radix bupleuri, 10-15 parts of hispid fig, 10-15 parts of fructus forsythiae, 5-10 parts of fritillaria, 10-15 parts of rhizoma atractylodis, 5-10 parts of lagotis and 5-15 parts of aconitum kusnezoffii.

7. The environment-friendly fungus preservation solution according to claim 5 or 6, wherein the traditional Chinese medicine antibacterial aqueous extract is prepared by the following method: weighing the raw materials according to the proportion, cleaning, drying, crushing, uniformly mixing, soaking in deionized water according to the solid-to-liquid ratio of 1:10, extracting for 1-2h by microwave heating at 500-700W, filtering, and concentrating the liquid by reduced pressure distillation until the density is 1.2-1.5g/cm³To obtain the traditional Chinese medicine antibacterial water extract.

8. The preservation solution for environmental friendly fungi as claimed in claim 1, wherein the stabilizer is selected from MgSO₄、NaCl、MgCl₂One or more of mannitol, sorbitol or sucrose.

9. The preservation solution for environmental protection fungi according to claim 1, wherein the fixing agent is one or more selected from glutaraldehyde, acrolein, tannic acid or potassium permanganate.

10. A method for preparing an environmentally friendly fungal preservation solution according to any one of claims 1 to 9, comprising the steps of: dissolving the traditional Chinese medicine antibacterial water extract and vitamin C in deionized water, adding a stabilizer, a fixing agent and glycerol, ultrasonically dispersing uniformly, and then dropwise adding acetic acid to adjust the pH value of the solution to 3.8-4.2.

Technical Field

The invention relates to the technical field of fungus preservation, in particular to an environment-friendly fungus preserving fluid and a preparation method thereof.

Background

The natural fungi have various types, and many types can form sporocarp, the shape of the sporocarp is complex, the shape of the sporocarp is different due to different fungi, and the sporocarp has an umbrella shape, a pen shape, a head shape, an ear shape, a tongue shape, a ball shape, a flower shape, a tree shape and the like, wherein most of the natural fungi belong to the types of external umbrella-shaped fungi, and are often called as agaricus, such as Ceriporiopsidae, amanitaceae, Agaricus campestris and the like; a small number of non-umbrella-shaped porous fungi and ascomycetes belong to Auricularia, Lasiosphaera, Morchella, Clavicipitaceae, etc.

The preservation method of the fungus fruiting body specimen mainly comprises two methods, one method is a traditional dry specimen preservation method, after the specimen is dried, the shape change of the fruiting body is large, the shape and the color change of the fruiting body are obvious, and the specimen is easy to be damaged by worms or mildewed, so that the specimen preservation or the subsequent research is not facilitated; in another method, preservation is carried out by adopting preservation solution, mainly formaldehyde solution, the shape of the fungus fruiting body is basically not changed, and the specimen is preserved better. The solution for soaking the fungus sporocarp into the specimen is mainly formaldehyde solution, but formaldehyde is volatile and can be oxidized and polymerized to precipitate, so that the concentration is reduced, the specimen is not favorably stored, the important factor is that the solution has a large toxic effect on a human body, and the preparation of the specimen preservation solution which can preserve the fungus sporocarp specimen for a long time and is safe and nontoxic is particularly important.

Disclosure of Invention

The invention provides an environment-friendly fungus preservation solution and a preparation method thereof, and aims to provide the environment-friendly fungus preservation solution.

The invention provides an environment-friendly fungus preservation solution which is prepared from the following raw materials: traditional Chinese medicine antibacterial water extract, vitamin C, stabilizer, fixative, glycerol, acetic acid and deionized water; the pH value of the environment-friendly fungus preservation solution is between 3.8 and 4.2.

As a further improvement of the invention, the health-care food is prepared from the following raw materials in parts by weight: 2-5 parts of traditional Chinese medicine antibacterial water extract, 2-7 parts of vitamin C, 1-3 parts of stabilizer, 1-2 parts of fixative, 10-20 parts of glycerol, a proper amount of acetic acid and 100 parts of deionized water, wherein the acetic acid is used for adjusting the pH value of the preservation solution.

As a further improvement of the invention, the health-care food is prepared from the following raw materials in parts by weight: 3-4 parts of traditional Chinese medicine antibacterial water extract, 3-6 parts of vitamin C, 2-3 parts of stabilizer, 1.2-1.7 parts of fixative, 12-18 parts of glycerol, a proper amount of acetic acid and 130 parts of deionized water, wherein the acetic acid is used for adjusting the pH value of the preservation solution.

As a further improvement of the invention, the health-care food is prepared from the following raw materials in parts by weight: 3.2 parts of traditional Chinese medicine antibacterial aqueous extract, 5 parts of vitamin C, 2.4 parts of stabilizing agent, 1.5 parts of fixing agent, 16 parts of glycerol, a proper amount of acetic acid and 125 parts of deionized water, wherein the acetic acid is used for adjusting the pH value of the preservation solution.

As a further improvement of the invention, the traditional Chinese medicine antibacterial aqueous extract is prepared from the following raw materials: galla chinensis, radix Isatidis, herba Taraxaci, bupleuri radix, hispid Fig, fructus forsythiae, Bulbus Fritillariae Cirrhosae, rhizoma Atractylodis, herba Lagotis and radix Aconiti Kusnezoffii.

As a further improvement of the invention, the traditional Chinese medicine antibacterial aqueous extract is prepared from the following raw materials in parts by weight: 5-10 parts of gallnut, 10-15 parts of isatis root, 5-15 parts of dandelion, 5-10 parts of radix bupleuri, 10-15 parts of hispid fig, 10-15 parts of fructus forsythiae, 5-10 parts of fritillaria, 10-15 parts of rhizoma atractylodis, 5-10 parts of lagotis and 5-15 parts of aconitum kusnezoffii.

As a further improvement of the invention, the traditional Chinese medicine antibacterial aqueous extract is prepared by the following method: weighing the raw materials according to the proportion, cleaning, drying, crushing, uniformly mixing, soaking in deionized water according to the solid-to-liquid ratio of 1:10, extracting for 1-2h by microwave heating at 500-700W, filtering, and concentrating the liquid by reduced pressure distillation until the density is 1.2-1.5g/cm³To obtain the traditional Chinese medicine antibacterial water extract.

As a further improvement of the invention, the stabilizer is selected from MgSO₄、NaCl、MgCl₂One or more of mannitol, sorbitol or sucrose.

As a further improvement of the invention, the fixing agent is selected from one or more of glutaraldehyde, acrolein, tannic acid or potassium permanganate.

The invention further provides a method for preparing the environment-friendly fungus preservation solution, which comprises the following steps: dissolving the traditional Chinese medicine antibacterial water extract and vitamin C in deionized water, adding a stabilizer, a fixing agent and glycerol, ultrasonically dispersing uniformly, and then dropwise adding acetic acid to adjust the pH value of the solution to 3.8-4.2.

The invention has the following beneficial effects: according to the invention, by adding the traditional Chinese medicine antibacterial aqueous extract, the aqueous extract obtained by the combined antibacterial traditional Chinese medicine has a good effect of killing bacteria, has a broad-spectrum and efficient effect, but has little power on fungi, and is suitable for being used as a preservation solution additive of the fungi;

according to the invention, by adding the stabilizer and the fixing agent, the form of the fungi is effectively kept unchanged, the later-stage research work is facilitated, meanwhile, no toxic volatile substance is added into the preservation solution, environment-friendly water is used as a solvent, the use is safe, no toxic or side effect is caused, the preservation and the storage are convenient, and the application prospect is wide.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.