阅读说明：本技术 制备pH依赖性抗体的方法 (Method for producing pH-dependent antibodies ) 是由 克里斯托弗·布朗什托 埃里克·霍夫曼 乔纳斯·德哈德 雅各布斯·科内利斯·拉塞尔 于 2018-05-10 设计创作，主要内容包括：公开了用于制备表现出改善的pH依赖性抗原结合的工程化抗体的方法。所述方法基于在抗体CDR内的限定的氨基酸位置的子集处引入组氨酸残基。用于组氨酸替换的所选氨基酸位置的组来源于来自天然抗体库之功能性抗体的CDR内的组氨酸存在的热图。所述方法提供了鉴定pH依赖性抗体变体的更简单且耗时更少的方法。(Methods for making engineered antibodies that exhibit improved pH-dependent antigen binding are disclosed. The method is based on the introduction of histidine residues at a subset of defined amino acid positions within the CDRs of the antibody. The set of selected amino acid positions for histidine substitutions was derived from a heatmap of histidine presence within the CDRs of functional antibodies from the natural antibody repertoire. The method provides a simpler and less time-consuming method of identifying pH-dependent antibody variants.)