阅读说明：本技术 制备水性透明质酸凝胶的方法 (Method for preparing aqueous hyaluronic acid gel ) 是由 F·贝尔泰恩 A·盖里 C·塞卡尔迪 于 2018-05-17 设计创作，主要内容包括：根据第一方面,本发明涉及制备水性透明质酸凝胶的方法,其包括以下步骤：a)制备交联水性透明质酸凝胶,b)通过压延均质化步骤(a)中形成的所述水性凝胶,c)中和步骤(b)中均质化的所述水性凝胶。根据第二方面,本发明还涉及能够通过这种方法获得的水性透明质酸凝胶。根据第三方面,本发明进一步涉及这种水性凝胶在修复或重建组织,特别是用于填充皱纹和细纹中的美容用途,或所述水性凝胶用于组织修复或重建的医疗用途。(According to a first aspect, the present invention relates to a process for preparing an aqueous hyaluronic acid gel, comprising the steps of: a) preparing a cross-linked aqueous hyaluronic acid gel, b) homogenizing the aqueous gel formed in step (a) by calendering, c) neutralizing the aqueous gel homogenized in step (b). According to a second aspect, the invention also relates to an aqueous hyaluronic acid gel obtainable by such a method. According to a third aspect, the invention further relates to the cosmetic use of such an aqueous gel in the repair or reconstruction of tissue, in particular for filling wrinkles and fine lines, or the medical use of said aqueous gel for tissue repair or reconstruction.)

1. A method of preparing an aqueous hyaluronic acid gel comprising the steps of:

a) preparing the water-based cross-linked hyaluronic acid gel,

b) homogenizing the aqueous gel formed in step (a) by calendering,

c) neutralizing the aqueous gel homogenized in step (b).

2. The method according to claim 1, wherein said calendering comprises a continuous compression between at least two counter-rotating rollers, preferably between three counter-rotating rollers.

3. A method according to any one of claims 1 and 2, characterized in that the spacing between the counter-rotating rollers is 20 μm-1mm, preferably 20 μm-100 μm.

4. The method according to any one of claims 1 to 3, characterized in that step a) of preparing the aqueous cross-linked hyaluronic acid gel comprises at least cross-linking the hyaluronic acid in an acid or alkaline medium in the presence of at least one cross-linking agent.

5. The method according to claim 4, characterized in that said cross-linking of the hyaluronic acid in an alkaline medium comprises at least the following steps:

-dissolving at least one hyaluronic acid and/or at least one salt thereof in an alkaline solution having a pH greater than 7.5, preferably greater than or equal to 10, more preferably between 10 and 14,

-crosslinking the hyaluronic acid in an alkaline solution in the presence of at least one crosslinking agent.

6. The method according to claim 4, wherein said cross-linking of said hyaluronic acid in an acid medium comprises at least the following steps:

-dissolving at least one hyaluronic acid and/or at least one salt thereof in an acid solution having a pH of less than 6.5, preferably less than or equal to 5, more preferably between 4.5 and 2,

-crosslinking the hyaluronic acid in an acid solution in the presence of at least one crosslinking agent.

7. The method according to any one of claims 4 to 6, wherein the step of cross-linking the hyaluronic acid comprises at least cross-linking the hyaluronic acid in an alkaline medium and cross-linking the hyaluronic acid in an acid medium, preferably by first cross-linking the hyaluronic acid in an alkaline medium and subsequently cross-linking the hyaluronic acid in an acid medium.

8. The method according to any one of claims 1 to 7, characterized in that step c) of neutralizing the hyaluronic acid solution is carried out by adjusting the pH to a pH of 6.5-7.5.

9. The process according to any one of claims 1 to 8, characterized in that the molar mass of the hyaluronic acid used for preparing the aqueous gel in step a) is from 1000000Da to 5000000Da, preferably from 1500000Da to 3500000 Da.

10. The method according to any one of claims 1 to 9, wherein the amount of hyaluronic acid in the aqueous hyaluronic acid gel obtained in step a) is between 1mg/ml and 300mg/ml, preferably between 75mg and 200mg/ml, more preferably between 100 and 175 mg/ml.

11. The method according to any one of claims 4 to 10, characterized in that the crosslinking agent is selected from difunctional epoxides, multifunctional epoxides, difunctional or multifunctional esters, divinyl sulphone, carbodiimides, formaldehyde, dialdehydes and mixtures thereof, preferably the crosslinking agent is 1, 4-butanediol diglycidyl glycerol ether (BDDE).

12. The method according to any one of claims 4 to 11, wherein in step i, the amount of crosslinking agent added is from 10mg to 250mg per gram of linear hyaluronic acid added.

13. The method according to any one of claims 5 to 12, wherein the salt of hyaluronic acid is selected from the group consisting of sodium, calcium, zinc and potassium salts, preferably sodium salt.

14. The process according to any one of claims 1 to 13, characterized in that the aqueous gel is purified, preferably by dialysis, before or after step b) of homogenization by calendering.

15. The process according to any one of claims 1 to 14, characterized in that linear hyaluronic acid is added before or after the above homogenization by calendering step b), neutralization (dilution) step c) or purification step.

16. The method of any one of claims 1-15, wherein the aqueous gel prepared is injectable.

17. An aqueous hyaluronic acid gel obtainable by the method according to any of claims 1-16.

18. Medical use of the aqueous gel according to claim 15 for repairing or reconstructing tissue.

19. Cosmetic use of the aqueous gel according to claim 17 for repairing or reconstructing tissue, in particular for filling wrinkles and fine lines.

Technical Field

The present invention relates to a process for preparing homogeneous aqueous hyaluronic acid gels, the aqueous gels thus obtained and their uses, in particular for filling wrinkles and fine lines.

Background

Collagen has long been the filling product of choice for facial applications, particularly for filling wrinkles and fine lines, or for reshaping the lips. However, hyaluronic acid is increasingly used with the sale of hyaluronic acid. In fact, in addition to the biodegradability of collagen, which is considered too fast, there are also safety issues related to its animal (bovine or porcine) origin.

Injection of hyaluronic acid has two advantages: immediate mechanical filling action and absence of inflammatory phenomena due to its biocompatibility. When administered in a linear (non-crosslinked) form, hyaluronic acid has excellent biocompatibility, but is degraded by the body very quickly (within about one week). By using cross-linked hyaluronic acid, the lifetime of hyaluronic acid-based injection products can be significantly extended to about 12 months. Indeed, the cross-linked hyaluronic acid is in the form of a cohesive gel with viscoelastic properties, which is particularly advantageously used in wrinkle-filling products.

However, during crosslinking, "hard domain" particles can form within the hyaluronic acid gel, rendering it heterogeneous. These hard areas affect the injectability of the product and are prone to cause tolerability problems for the patient. Thus, these hard zones must be eliminated from the cross-linked hyaluronic acid gel in order to obtain a completely homogeneous product for its administration. Conventionally, cross-linked hyaluronic acid gels are homogenized by sieving or extrusion. These methods allow only partial elimination of hard regions. In addition, the shear stress exerted on the gel causes deterioration of its structure and its viscoelasticity. Thus, the sieved, filtered or extruded gel is not completely homogeneous and the viscosity is reduced. Once injected, it runs the risk of migrating into the tissue and degrading more rapidly. Therefore, the filling property thereof is degraded.

Therefore, there is a need to obtain a method capable of effectively eliminating the hard domains present in the crosslinked hyaluronic acid gel, thereby obtaining a homogeneous gel without deteriorating its viscoelasticity.

Disclosure of Invention

The present invention therefore proposes a process for the preparation of a homogeneous cross-linked hyaluronic acid gel, wherein homogenization of the gel is obtained by calendering (roll).

In particular, according to a first aspect, one subject of the present invention is a process for preparing an aqueous hyaluronic acid gel, comprising the following steps:

a) preparing the water-based cross-linked hyaluronic acid gel,

b) homogenizing the aqueous gel formed in step (a) by calendering,

c) neutralizing the aqueous gel homogenized in step (b).

According to a second aspect, another subject of the invention is an aqueous hyaluronic acid gel obtainable by this method.

According to a third aspect, another subject of the invention is the cosmetic use of such an aqueous gel in the repair or reconstruction of tissues, in particular for filling wrinkles and fine lines, or the medical use of said aqueous gel for repairing or reconstructing tissues.

Drawings

Figure 1 shows gel calendering between two rolls rotating at the same tangential speed.

Figure 2 shows gel calendering between three rolls with increasing tangential velocity to enable entrainment of gel on the surface of adjacent rolls.

Fig. 3, 4 and 5 show the ejection force of the aqueous gel compositions 3, 4 and 5 prepared in the exemplary embodiment.

Aqueous gel

The invention provides a novel method for preparing aqueous hyaluronic acid gel.

For the purposes of the present application, "gel" is understood to mean a cohesive composition which does not flow under its own weight and has viscoelastic properties which impart a certain deformability to it. If the gel is sheared, it does not reform as a viscous fluid.

Therefore, the hyaluronic acid gel according to the present invention is different from the hyaluronic acid solution. The gel/solution difference can be observed by rheological studies at strain and constant frequency at 25 ℃ to determine the viscous modulus G "and the elastic modulus G' in the linear viscoelastic region. In particular, within the meaning of the present invention, gels are characterized in particular by the fact that: its elastic modulus G 'is greater than the viscous modulus G' defined according to Winter and Chambon (1986). On the other hand, in the case of a viscous solution, the viscous modulus G "is greater than its elastic modulus G'.

Measurements were made according to continuous mode (10% strain at 25 ℃, frequency 1Hz, duration 120s) on a Discovery HR1(TA Industries) rheometer and a 40mm plate/plate geometry. About 1.2ml of sample was deposited in a 1000 μm gap.

The hyaluronic acid gel according to the invention is preferably homogeneous. For the purposes of the present invention, "homogeneous hyaluronic acid gel" is understood to mean that the crosslinked hyaluronic acid is homogeneously dispersed within the gel.

In particular, the homogeneity of the hyaluronic acid gel can be characterized by measuring the variation in the force with which the gel is ejected through a syringe, the needle of which has an internal diameter of 300 μm (27G TSK UTW). The measurement of the ejection force (or extrusion force) was carried out by means of a Shimadzu EZ-Test SX force-measuring platform equipped with 50 NCell. Extrusion at 10mm.min^-1Is performed and the sampling is set to 100 points s^-1. The test was performed using a 1ml long BD syringe fitted with an 1/2"TSK 27G needle. The acquisition was performed from 20 th to 140 th seconds of extrusion to not consider the contact stress at the start and end of extrusion. At the end of the acquisition, a series of points N ═ f (t) (extrusion force as a function of time) was linearized. The margin with respect to linearity is depicted as ± 10%. Each intersection of the curve N ═ f (t) with the linearization N ═ f (t) lines N +10 ═ f (t) and N-10 ═ f (t) corresponds to the extrusion of the heterogeneous fraction.

Thus, according to a preferred embodiment, the extrusion force of the homogeneous hyaluronic acid gel does not vary by more than ± 10% with respect to the linear extrusion force.

Hyaluronic acid

The aqueous gel according to the invention comprises at least one hyaluronic acid.

Hyaluronic acid is a linear glycosaminoglycan (GAG) composed of repeating units of D-glucuronic acid and N-acetyl-D-glucosamine joined together by alternating β -1,4 and β -1,3 glycosidic linkages.

Hyaluronic acid has the following structure:

preferably, the hyaluronic acid used in the preparation of the aqueous gel according to the invention has a molar mass of 1000000Da-5000000Da, preferably 1500000Da-3500000 Da. In particular, the molecular weight can be determined by Waters GPCV Alliance 2000 exclusion chromatography, eluent: 0.1M NaNO₃Dissolved in water and measured in tandem with three Wyatt detectors (refractometer, viscometer and light scatterometer).

Hyaluronic acid is present in the aqueous hyaluronic acid gel obtained in step a) in a content of from 1mg/ml to 300mg/ml, preferably from 75mg/ml to 200mg/ml, more preferably from 100mg/ml to 175 mg/ml.

Aqueous phase

In addition to hyaluronic acid, the aqueous gel according to the invention comprises an aqueous phase.

The gel may comprise water in an amount of 60% to 99% by weight, preferably 70% to 99% by weight, and preferably 80% to 99% by weight, relative to the total weight of the composition.

The gel may further comprise a polyol miscible with water at ambient temperature (25 ℃), in particular chosen from polyols having from 2 to 20 carbon atoms, preferably from 2 to 10 carbon atoms, more preferably from 2 to 6 carbon atoms, such as glycerol, propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, dipropylene glycol, diethylene glycol; glycol ethers (in particular having 3 to 16 carbon atoms), such as mono-, di-or tripropylene glycol (C1-C4) alkyl ethers, mono-, di-or triethylene glycol (C1-C4) alkyl ethers; and mixtures thereof.

The water-miscible polyol may be present in the gel of the invention in a content ranging from 0.1% to 20% by weight, preferably from 3% to 15% by weight, relative to the total weight of the composition.

Preparation of aqueous Cross-Linked hyaluronic acid gel

The first step a) of preparing the aqueous cross-linked hyaluronic acid gel is carried out according to the method of the invention.

This step a) preferably comprises at least cross-linking the hyaluronic acid in an acid or alkaline medium in the presence of at least one cross-linking agent.

According to a particular embodiment, the crosslinking is carried out in an alkaline medium and comprises at least the following steps:

-dissolving at least one hyaluronic acid and/or at least one salt thereof in an alkaline solution having a pH greater than 7.5, preferably greater than or equal to 10, more preferably between 10 and 14,

-crosslinking the hyaluronic acid in an alkaline solution in the presence of at least one crosslinking agent.

Crosslinking in alkaline medium favours the formation of ether linkages between hyaluronic acid and the crosslinker, which slowly degrade.

According to another particular embodiment, the crosslinking is carried out in an acid medium and comprises at least the following steps:

-dissolving at least one hyaluronic acid and/or at least one salt thereof in an acid solution having a pH of less than 6.5, preferably less than or equal to 5, more preferably between 4.5 and 2,

-crosslinking the hyaluronic acid in an acid solution in the presence of at least one crosslinking agent.

Crosslinking in an acid medium itself favors the formation of ester bonds between the hyaluronic acid and the crosslinker, which degrade faster than ether bonds.

According to a preferred embodiment, the step of cross-linking the hyaluronic acid comprises at least cross-linking the hyaluronic acid in an alkaline medium and cross-linking the hyaluronic acid in an acid medium to control the ether and ester bonds formed and the degradation rate of the cross-linked hyaluronic acid gel so formed.

More preferably, the step of cross-linking the hyaluronic acid comprises first cross-linking the hyaluronic acid in an alkaline medium and subsequently cross-linking the hyaluronic acid in an acid medium.

Prior to dissolution, the hyaluronic acid used in the method according to the invention is typically in dry form, preferably in powder or flake form.

When used in salt form, the hyaluronic acid may preferably be a sodium, calcium, zinc or potassium salt of hyaluronic acid, and preferably a sodium salt.

The amount of linear hyaluronic acid dissolved in the aqueous solution (corresponding to the amount of hyaluronic acid in step a) is between 50mg/ml and 300mg/ml, preferably 100 and 200 mg/ml.

The crosslinking of the linear hyaluronic acid dissolved in the aqueous solution is carried out in the presence of at least one crosslinking agent.

The crosslinking agent is preferably selected from the group consisting of difunctional epoxides, multifunctional epoxides, difunctional or multifunctional esters, divinyl sulfones, carbodiimides, formaldehyde, dialdehydes and mixtures thereof, preferably the crosslinking agent is 1, 4-butanediol diglycidyl triol ether (BDDE, also known as 1, 4-diglycidyl oxybutane, tetramethylene glycol diglycidyl triol ether and IUPAC name 2- [4- (oxiran-2-ylmethoxy) butoxymethyl ] oxirane).

In the crosslinking step, the amount of the crosslinking agent added is particularly 10mg to 250mg per gram of the linear hyaluronic acid added.

The crosslinking step is preferably carried out at a temperature of from 30 ℃ to 70 ℃, preferably from 45 ℃ to 55 ℃, which makes it possible to catalyze the crosslinking of the hyaluronic acid.

Homogenizing by calendering

Then, within the scope of the process of the invention, the aqueous cross-linked hyaluronic acid gel prepared in step a) is homogenized by calendering in order to eliminate the hard zones (aggregates formed during cross-linking) without reducing the mechanical and viscoelastic properties of the gel.

In particular, calendering comprises a continuous compression between at least two counter-rotating rolls, preferably three counter-rotating rolls.

For example, the feed rollers may be at 0.1m.s^-1-5m.s^-1Preferably 0.5m.s^-1-3m.s^-1Is rotated at the tangential speed of the rotor.

When calendering is performed between two rolls, they are preferably rotated at the same tangential speed and the gel is fed between the two rolls, as shown in fig. 1.

When calendering is performed between three rolls, the tangential velocity of each roll should be increased to enable entrainment of the gel onto the surface of the second roll for a second calendering between the second and third rolls. For example, as shown in fig. 2, if a first roll is rotated at a tangential velocity × 1, a second roll may be rotated at the tangential velocity × 2, and a third roll may be rotated at the tangential velocity × 3 to enable double calendering of the gel.

According to a preferred embodiment, the spacing between the counter-rotating rollers (also called gap) is between 20 μm and 1mm, preferably between 20 μm and 100 μm.

Preferably, the roller may be made of stainless steel so as to be able to be easily cleaned, and is optionally provided with a micro-porous or ceramic coating so as to be able to promote the adhesion of the gel to the roller surface.

According to a preferred embodiment, step b) of homogenization by calendering is carried out for a time ranging from 1 minute to 2 hours, preferably from 15 minutes to 45 minutes. Within the scope of the present invention, these relatively short calendering times can be used as long as step b) of homogenizing the aqueous gel is carried out before neutralization step c), which leads to swelling of said aqueous gel. In particular, the volume of the aqueous gel to be homogenized by calendering is significantly reduced before swelling compared to after swelling.

Purification of

According to a particular embodiment, in particular in the case of neutralization without dialysis, the aqueous crosslinked hyaluronic acid gel may be purified before or after step c) of neutralizing the aqueous gel, in order to eliminate traces of residual crosslinking agent.

According to a preferred embodiment, the purification is preferably carried out by dialysis under the conditions described above.

Purification by dialysis makes it possible, in addition to elimination of residual crosslinking agent, to further refine the pH obtained after neutralization and to control the osmotic pressure of the gel.

Purification may result in a new dilution of the hyaluronic acid. The content of cross-linked hyaluronic acid present in the gel after purification is between 1mg/ml and 60mg/ml, preferably between 5 and 50 mg/ml.

Neutralization

After homogenization by calendering, the aqueous hyaluronic acid gel is neutralized during step c).

Neutralization is carried out by adjusting the pH to a pH of 6.5-7.5. This neutralization can be carried out by adding an acid or a base, depending on whether the crosslinking is carried out in a base or acid medium.

Neutralization results in dilution of the hyaluronic acid. The content of the cross-linked hyaluronic acid present in the gel after neutralization is from 10mg/ml to 100mg/ml, preferably from 20 to 80 mg/ml.

For example, when crosslinking has been carried out in an acid medium, the pH can be adjusted by adding compounds such as ammonium hydroxide, sodium bicarbonate, baking soda, sodium carbonate or derivatives thereof or phosphate buffered solution (PBS "phosphate buffered saline").

When the crosslinking has been performed in an alkaline medium, pH adjustment for neutralization may be performed by adding compounds such as hydrochloric acid, acetic acid, phosphoric acid, and sodium dihydrogen phosphate or derivatives thereof.

Alternatively, neutralization may be performed by dialysis. Neutralization by dialysis enables the pH to be adjusted in a very gradual manner, which makes it possible to optimally maintain the mechanical and viscoelastic properties of the formed hyaluronic acid gel.

Dialysis is a membrane separation process used to separate molecules or ions in solution. Thus, in the context of the present application, the hyaluronic acid gel according to the invention can be dialyzed against a buffer solution having a pH equal to or close to the desired final pH (target pH) of the hyaluronic acid gel, i.e. between 6.5 and 7.5, preferably between 6.75 and 7.2.

For example, the buffer solution may be a phosphate buffered saline (PBS, PBS-lactic acid) solution, TRIS (hydroxymethyl) methylamine (TRIS) solution, TRIS salt solution (TBS), 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid (HEPES) solution, 2- { [ TRIS (hydroxymethyl) -methyl ] -amino } ethanesulfonic acid (TES) solution, 3- (N-morpholino) -propane-sulfonic acid (MOPS) solution, piperazine-N, N' -bis (2-ethanesulfonic acid) solution, MES (2- (N-morpholino) ethanesulfonic acid (PIPES) solution, and sodium chloride (NaCl) solution.

According to a preferred embodiment, the buffer solution is formed from the "acid" salt NaH₂PO₄Alkali salt Na₂HPO₄And NaCl (phosphate buffered saline) solution.

According to a particular embodiment, the buffer is physiologically acceptable, i.e. it presents no risk of intolerance or toxicity when the hyaluronic acid gel according to the invention is injected or brought into contact with the tissue.

According to a particular embodiment of the invention, dialysis can be carried out in one or several baths with a buffer solution as described above.

According to a more preferred embodiment, dialysis can be performed in a plurality of successive baths with buffer solutions having different pH values, which are increasingly closer to the desired final pH (target pH) of the hyaluronic acid solution. Thus, the pH can be increased more gradually depending on the number of buffer baths used.

According to a preferred embodiment, in order to simultaneously control the osmotic pressure of the hyaluronic acid gel, the buffer used for dialysis may be mixed with so-called neutral salts (i.e. neutral salts that do not interact with the buffer, in particular salts with a concentration of up to 280mOsmol. l^-1-380mOsmol.l^-1Sodium salt (NaCl) or potassium salt (KCl)) of tissue osmolarity.

In particular, the osmotic pressure of the buffer solution may be 250-350mOsm/l, preferably 280-330 mOsm/l.

In the context of the present invention, the neutralization step c) results in swelling of the crosslinked hyaluronic acid gel. In general, this swelling leads in particular to a 2-4 fold increase in the volume of the hyaluronic acid gel relative to the volume of the aqueous crosslinked hyaluronic acid gel obtained in step a).

It is important within the scope of the invention that, before the calendering step b), the swelling (and therefore the neutralization) does not start or starts to be small enough to enable the use of a sufficiently narrow gap (20 μm-1mm) to allow the effective elimination of the hard zones (aggregates formed during crosslinking) without reducing the mechanical and viscoelastic properties of the gel.

In particular, if calendering is carried out simultaneously (for example as described in document US 2013/0217872), or after neutralization and therefore after swelling of the gel, the spacing between the rolls of the calender must be significantly increased to allow passage of the swollen gel occupying a greater volume. Such a spacing between the rollers of more than 1mm no longer effectively eliminates the hard domains present in the gel to ensure its excellent homogeneity and injectability. In this case, document US2013/0217872 does not describe injectable hyaluronic acid gels and also does not seek to eliminate the hard zones present in gels by calendering. In this document, calendering enables mixing of the neutralized gel for a very long time of 18-24h to achieve swelling equilibrium.

In order to provide a gel obtained by the method according to the invention with good injectability, it is preferred that the gel does not contain hard zones with a diameter of more than 1mm, preferably more than 20 μm.

Linear hyaluronic acid

According to a particular embodiment, the linear hyaluronic acid may be added after step (a) of preparing the aqueous cross-linked hyaluronic acid gel, in order to reduce the viscosity of the gel and thus to adjust its mechanical properties, in particular to reduce the ejection force of the gel and to facilitate filling of the syringe.

The addition of the linear hyaluronic acid may be carried out before or after the above-mentioned homogenization by calendering step b), neutralization (dilution) step c) or purification step. According to a preferred embodiment, the addition of the linear hyaluronic acid may be carried out before or after the above-mentioned purification step.

The amount of linear hyaluronic acid added to the cross-linked hyaluronic acid gel is preferably less than or equal to the amount of cross-linked hyaluronic acid present in the gel after neutralization and optional purification, so as not to further dilute the hyaluronic acid.

In particular, the cross-linked hyaluronic acid is present in the gel after purification in an amount of 0.1mg/ml to 100mg/ml, preferably 1mg to 50 mg/ml.

Other polymers

According to a particular embodiment, the aqueous hyaluronic acid gel further comprises at least one other polymer than hyaluronic acid, such as chondroitin, cellulose, alginate, polycaprolactone, polylactic acid, polyglycolic acid, collagen, silk, PTFE and derivatives thereof.

The further polymer may be added during step a) before cross-linking the hyaluronic acid, or after step a) of preparing the aqueous cross-linked hyaluronic acid gel, in particular before step b) of homogenization by calendering, so as to co-cross-link the hyaluronic acid with the further polymer.

For example, other polymers may be added at levels of 0.1% to 5%, preferably 0.5% to 4%.

Injectable composition

According to a preferred embodiment, the aqueous gel prepared according to the process of the invention is injectable.

For the purposes of the present invention, an injectable gel is understood to mean a composition in the form of a gel which has satisfactory injectability (or injectability, i.e. easy to inject due to a more or less satisfactory flow through the needle into the syringe), in particular capable of being injected by means of a syringe having a needle with an internal diameter of approximately 300 μm. For the purposes of the present application, the injectable gel preferably has a viscosity of less than or equal to 10000pa.s and a loss factor (Tan δ) of from 0.01 to 5, from a rheological point of view.

Rheological measurements (G', G "and Tan. delta.) were made according to the continuous mode (10% strain at 25 ℃ with a frequency of 1Hz for 120s) on a Discovery HR1(TA Industries) rheometer and a 40mm plate/plate geometry. About 1.2ml of sample was deposited in a 1000 μm gap.

The maximum viscosity measurement is carried out in dynamic mode (angular frequency 0.1-100rad. s)^-1). About 1.2ml of sample was deposited in a 1000 μm gap.

Thus, the aqueous gel prepared according to the method of the present invention may be contained in a syringe to enable injection into a tissue.

Thus, according to another aspect, the subject of the invention is a syringe containing a gel prepared according to the process of the invention as described above. Such syringes are particularly intended for filling wrinkles or fine lines.

According to this embodiment, degassing may be performed prior to filling the syringe to eliminate any possible bubbles.

Use of

In a particular embodiment, the aqueous gel obtained according to the invention is intended for use in repairing or reconstructing tissue.

In particular, the aqueous gel according to the invention can be used to form or replace biological tissues, for example as an implant; or to fill biological tissue, such as by injection into osteochondral or joints; or for filling cavities of the body or face, such as wrinkles or fine lines; for creating or increasing a volume of a human body or face; or for skin healing.

According to other particular embodiments, the aqueous gel according to the invention can be used for:

surgery, in particular organ repair or cosmetic surgery or medicine,

urology, in particular for the treatment of urinary incontinence,

infectious diseases, in particular as carrier liquid for vaccines,

ophthalmology, in particular for corneal healing,

dentistry, in particular for implanting dental implants or for bone repair,

orthopedics, in particular for creating a volume in the periosteum,

cell therapy or tissue engineering within the scope of vectorization of therapeutic cells or dual-acting factors,

or vasculology.

The aqueous gel according to the invention can also be used for rheumatism.

Advantageously, the aqueous gel according to the invention can also be used as a carrier for active ingredients, in particular therapeutically active ingredients (such as cells, vaccines or hormones of the insulin or estrogen type), more generally for all active ingredients, the controlled and/or prolonged release or delivery of which is advantageous.

The invention also relates to the cosmetic use of the aqueous gel according to the invention for treating or combating skin ageing.

The following examples are intended to illustrate the invention without limiting its scope in any way.

Examples

The aqueous hyaluronic acid gel according to the invention (composition 1) was prepared according to the following method:

hyaluronic acid (HTL, France) was completely dissolved in alkaline phosphate buffer solution (300 mOsmol)^-1pH 12.9, Merck, france) to obtain 150mg^-1The final hyaluronic acid concentration of (a).

A 20 wt% BDDE (SA, france) solution was slowly added. The mixture was then heated at 50 ℃ until the texture no longer changed and the mixture was dyed yellow.

The obtained gel was then calendered using an EXAKT 50i G-Line three-roll mill (Exakt, Germany).

When the calendering was complete, acidic phosphate buffer solution (472mosmol^-1pH 1.59) to neutralize the reaction mixture and dilute the gel to 32.5mg.ml^-1The hyaluronic acid concentration of (a).

Then, the gel was treated with phosphate buffer (300mOsmol. l)^-1pH 7.4, Merck, france). When neutrality is reached, dialysis is stopped. Finally, 4% (w/w) of 25mg.ml was added^-1Hyaluronic acid solution (HTL, france). The acidic gel was then placed in a 1ml syringe (BD, 1ml long) and then sterilized by autoclaving (121 ℃ for 15 min).

A comparative aqueous hyaluronic acid gel (composition 2) was prepared according to the same method, except for the calendering step which was not performed.

The effect of calendering on gel homogeneity was confirmed by measuring the jetting force. The more stable the spray force during the extrusion of the product through the syringe and needle, the more homogeneous the gel.

The ejection force of the aqueous gel compositions 1 and 2 prepared above was measured. The results of these measurements are shown in figures 3 and 4.

For composition 1 according to the invention, an excellent stability of the ejection force was observed (fig. 3). On the other hand, for uncalendered, unscreened and unground composition 2 (comparative), a large change in jetting force was observed, which was several times out of the ± 10% margin of linearized N ═ f (t) extrusion force.

The ejection force of a commercial composition, Teosyal ultrapeel (composition 3), of an aqueous cross-linked hyaluronic acid gel was also measured, its manufacturing method using a sieving/grinding step as described in patent application US 2013/0237615. The results of this measurement are shown in fig. 5. A large variation in ejection force was observed during ejection, indicating heterogeneity of the gel.