阅读说明：本技术 一种含探针阵列的毛细管及其应用和制备方法 (Capillary tube containing probe array and application and preparation method thereof ) 是由 韩卫华 黄小娜 于 2019-08-15 设计创作，主要内容包括：本发明提供了一种含探针阵列的毛细管及其应用和制备方法,涉及免疫诊断设备技术领域,包括毛细管,毛细管内壁设有检测区,检测区沿毛细管延伸方向间隔敷设有若干生物探针层,相邻两生物探针层间的毛细管内壁敷设有封闭剂层；本发明可以根据需要固定不同种类、数量和浓度的生物探针,固定过程简单方便；可用于多种指标的联合检测；所使用的载体是价格便宜的毛细管,成本极低。(The invention provides a capillary tube containing a probe array and an application and preparation method thereof, relating to the technical field of immunodiagnosis equipment and comprising a capillary tube, wherein the inner wall of the capillary tube is provided with a detection area, a plurality of biological probe layers are laid on the detection area at intervals along the extension direction of the capillary tube, and a sealant layer is laid on the inner wall of the capillary tube between two adjacent biological probe layers; the invention can fix biological probes with different types, quantities and concentrations according to the needs, and the fixing process is simple and convenient; the method can be used for joint detection of various indexes; the carrier used is a capillary tube with low price and extremely low cost.)

1. A capillary tube comprising an array of probes, wherein: the biological probe comprises a capillary tube, wherein a detection area is arranged on the inner wall of the capillary tube, a plurality of biological probe layers are laid on the detection area at intervals along the extension direction of the capillary tube, and a sealant layer is laid on the inner wall of the capillary tube between every two adjacent biological probe layers.

2. The probe array-containing capillary tube according to claim 1, wherein: the biological probe layer and the sealant layer are closed rings.

3. The probe array-containing capillary tube according to claim 1, wherein: and sealant layers are laid at two ends of the detection area on the inner wall of the capillary tube.

4. The probe array-containing capillary tube according to claim 1, wherein: the capillary tube is made of glass, quartz, silicon, metal, organic polymer materials or biological polymer materials.

5. The probe array-containing capillary tube according to claim 1, wherein: the inner diameter of the capillary tube is 0.001 mm-10 mm.

6. The probe array-containing capillary tube according to claim 1, wherein: the biological probe of the biological probe layer is nucleic acid, polypeptide, protein, antigen, antibody, polysaccharide, ligand or receptor.

7. A microfluidic chip, characterized in that: the capillary tube array comprises a shell and a plurality of capillary tubes containing the probe array according to any one of claims 1 to 6, wherein the plurality of capillary tubes are packaged in the shell, and the plurality of capillary tubes are mutually connected in series or in parallel.

8. A method for preparing a capillary tube containing a probe array is characterized by comprising the following steps: one end of the capillary tube is a leading-in end, and the other end of the capillary tube is a cleaning end, and the preparation steps are as follows:

1) fixing the biological probe:

s1, introducing a section of biological probe solution liquid column from the introduction end of a capillary;

s2, carrying out warm bath on the capillary for 0.5-1.5 h;

s3, pumping out the section of biological probe solution, introducing a buffer solution from the cleaning end of the capillary tube for cleaning and drying;

2) fixing a sealing agent:

s4, introducing a section of sealant solution liquid column from the introduction end of the capillary, wherein the section of sealant solution liquid column is longer than the biological probe solution liquid column in the previous step;

s5, carrying out warm bath on the capillary for 0.5-1.5 h;

s6, pumping out the section of sealant solution, introducing a buffer solution from the cleaning end of the capillary tube for cleaning and drying;

3) fixing the biological probe:

s7, introducing a section of biological probe solution liquid column from the introduction end of the capillary, wherein the section of biological probe solution liquid column is longer than the sealant solution liquid column in the previous step;

s8, carrying out warm bath on the capillary for 0.5-1.5 h;

s9, pumping out the section of biological probe solution, introducing a buffer solution from the cleaning end of the capillary tube for cleaning and drying;

and S10, repeating the operations 2) and 3) until the manufacturing is finished.

9. The method for preparing a capillary tube containing a probe array according to claim 8, wherein: the method further includes step S0. of pre-treating the inner wall of the capillary tube to couple an active group capable of linking with the biological probe to the inner wall of the capillary tube.

Technical Field

The invention relates to the technical field of immunodiagnosis equipment, in particular to a capillary containing a probe array and an application and preparation method thereof.

Background

The microfluidic technology refers to a technology for realizing various reactions, separations and detections by using a micro-pipeline or a micro-structure, and has the characteristics of time saving, low sample consumption, excellent detection performance and the like. Due to the above characteristics, the microfluidic chip has attracted much attention in the field of in vitro diagnosis and the like. The traditional microfluidic chip needs to process various microchannels and microstructures on a substrate by using a micro-processing technology, so that the technical difficulty and the processing cost are high, and the development and the application of the microfluidic chip product are seriously hindered.

Compared with the traditional microfluidic chip, the capillary has the advantages of small inner diameter, simple manufacture, low cost and the like, and is widely applied to the fields of chemical analysis, immunodiagnosis and the like. However, in the current application, one capillary tube is often fixed with only one probe, and the multi-index combined detection cannot be realized. It has been proposed to use a UV-decomposable substance as an intermediate phase for immobilizing a plurality of different probes in a single capillary, but the UV used in this method causes denaturation of proteins.

Disclosure of Invention

The first object of the present invention is to provide a capillary containing a probe array, which uses the capillary as a carrier, and simultaneously fixes a plurality of biological probes on the inner wall thereof, for single-index detection or combined detection of a plurality of indexes.

The second purpose of the invention is to provide a microfluidic chip which adopts the capillary tube containing the probe array.

The third object of the present invention is to provide a method for preparing a capillary tube containing a probe array, which can rapidly and stably prepare the capillary tube containing a probe array.

The embodiment of the invention is realized by the following steps:

a capillary tube containing a probe array comprises a capillary tube, wherein a detection area is arranged on the inner wall of the capillary tube, a plurality of biological probe layers are laid on the detection area at intervals along the extension direction of the capillary tube, and a sealant layer is laid on the inner wall of the capillary tube between every two adjacent biological probe layers.

Furthermore, the biological probe layer and the sealant layer are closed rings.

Furthermore, sealant layers are laid at two ends of the detection area on the inner wall of the capillary.

Furthermore, the capillary tube is made of glass, quartz, silicon, metal, organic polymer materials or biological polymer materials.

Furthermore, the inner diameter of the capillary tube is 0.001 mm-10 mm.

Further, the biological probe of the biological probe layer is nucleic acid, polypeptide, protein, antigen, antibody, polysaccharide, ligand or receptor.

A microfluidic chip comprises a shell and a plurality of capillaries containing probe arrays, wherein the capillaries are packaged in the shell, and the capillaries are mutually connected in series or in parallel.

A method for preparing capillary tube containing probe array includes setting one end of capillary tube as leading-in end and another end as cleaning end, preparing steps as follows:

1) fixing the biological probe:

s1, introducing a section of biological probe solution liquid column from the introduction end of a capillary;

s2, carrying out warm bath on the capillary for 0.5-1.5 h;

s3, pumping out the section of the biological probe solution, introducing a buffer solution from the cleaning end of the capillary tube for cleaning and drying to form a biological probe layer;

2) fixing a sealing agent:

s4, introducing a section of sealant solution liquid column from the introduction end of the capillary, wherein the section of sealant solution liquid column is longer than the biological probe solution liquid column in the previous step;

s5, carrying out warm bath on the capillary for 0.5-1.5 h;

s6, pumping out the section of sealant solution, introducing a buffer solution from the cleaning end of the capillary tube for cleaning and drying to form a sealant layer;

3) fixing the biological probe:

s7, introducing a section of biological probe solution liquid column from the introduction end of the capillary, wherein the section of biological probe solution liquid column is longer than the sealant solution liquid column in the previous step;

s8, carrying out warm bath on the capillary for 0.5-1.5 h;

s9, pumping out the section of the biological probe solution, introducing a buffer solution from the cleaning end of the capillary tube for cleaning and drying to form a biological probe layer;

and S10, repeating the operations 2) and 3) until the manufacturing is finished.

Further, step S0. is performed to pre-treat the inner wall of the capillary tube, so that the inner wall of the capillary tube is coupled with an active group capable of being linked with the biological probe.

The invention has the beneficial effects that:

the invention can fix biological probes with different types, quantities and concentrations according to the needs, and the fixing process is simple and convenient; the method can be used for joint detection of various indexes; the carrier used is a capillary tube with low price and extremely low cost.

Compared with the traditional microfluidic chip, the microfluidic chip manufactured by the capillary tube containing the probe array does not need to process a microchannel and a microstructure by using a micro-processing method, does not need to bond the chip, and greatly reduces the processing cost and difficulty.

The method for preparing the capillary tube containing the probe array has the advantages of large freedom degree of biological probe fixation, simple method, low material requirement, low cost and easy operation, and can effectively avoid cross contamination among biological probes.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a schematic view showing the distribution of a bioprobe layer and a blocking agent layer provided in example 1 of the present invention;

FIG. 2 is a partial cross-sectional view of a capillary containing an array of probes according to example 1 of the present invention;

icon: 1-capillary, 2-biological probe layer and 3-sealant layer.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or the orientations or positional relationships that the products of the present invention are conventionally placed in use, and are only used for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.