阅读说明：本技术 一种从泔料中提取氨基酸的方法 (Method for extracting amino acid from swill ) 是由 林金新 郭小雷 黄平 于 2019-10-09 设计创作，主要内容包括：本发明涉及氨基酸技术领域,公开了一种从泔料中提取氨基酸的方法：包括以下步骤：取泔料蚕蛹,经压榨去油并粉碎后相继用汽油浸泡1~2日,回收汽油,得脱脂蚕蛹粉；对脱脂蚕蛹粉进行水解、脱酸、脱色后,通过732阳离子树脂柱粗分,然后对中性氨基酸进行进一步分组；对酸性氨基酸进行分离。本发明提供了一种从泔料中提取氨基酸的方法,成本低,减少了废物的排放。氨基酸的回收率达到20-30%。(The invention relates to the technical field of amino acid, and discloses a method for extracting amino acid from swill, which comprises the following steps of taking swill silkworm chrysalis, squeezing to remove oil, crushing, soaking in gasoline for 1 ~ 2 days in sequence, recovering gasoline to obtain degreased silkworm chrysalis powder, hydrolyzing, deacidifying and decoloring the degreased silkworm chrysalis powder, roughly separating the degreased silkworm chrysalis powder through a 732 cationic resin column, further grouping neutral amino acid, and separating acidic amino acid.)

1. A method for extracting amino acid from swill is characterized by comprising the following steps:

s10, taking the hogwash silkworm chrysalis, squeezing to remove oil, crushing, soaking in gasoline for 1 ~ 2 days, and recovering the gasoline to obtain degreased silkworm chrysalis powder;

s20, carrying out acid hydrolysis on the degreased silkworm chrysalis powder;

s30, deacidifying the hydrolyzed degreased silkworm chrysalis powder;

s40, carrying out decolorization treatment on the deacidified degreased silkworm chrysalis powder liquid;

s50, carrying out column chromatography on the decolorized liquid through 732 cation exchange resin to obtain crude acidic amino acid, neutral amino acid and lysine;

s60, adjusting the pH value of the neutral amino acid component to 8 ~ 8.5.5, and separating the neutral amino acid component into components mainly comprising Gly, Ala and Leu and Val through 717 anion exchange resin column chromatography;

s70, adjusting the pH value of the acidic amino acid component to 5 ~ 6, and passing through a 717 anion exchange resin column to further divide the acidic amino acid component into Asp and Glu;

s80, adjusting pH to 8, passing through 717 anion exchange resin column, and further dividing into Gly and Ala;

s90, adjusting the pH value of the components mainly containing Leu and Val to 3.5 ~ 4.0.0, and separating the components into Leu and Val through 732 cation exchange resin column chromatography;

s100: finally, various amino acids are refined.

2. The method of claim 1, wherein the step of coarsely separating 732 cationic resin columns comprises:

firstly, leading the deacidification decoloration solution to pass through a 732 cationic resin column at the flow rate of 100 ~ 130ml/min until the deacidification decoloration solution is saturated;

secondly, eluting with 0.1mol/L ammonia water at the flow rate of 80 ~ 90ml/min, and collecting the eluates with a container in sequence until the pH value is 11;

the third step: and (3) sequentially performing paper chromatography on the eluent bottle by bottle, wherein the developing agent is phenol and water in a volume ratio of 3:1, and respectively combining acidic amino acid, neutral amino acid and lysine components.

3. The method according to claim 1 or 2, characterized in that the neutral amino acids are further grouped by the steps of:

firstly, adding carbon to decolor neutral amino acid components separated by a 732 cation column until the neutral amino acid components are colorless and clear, slowly adjusting the pH value to 8 ~ 8.5.5 by using dilute sodium hydroxide, and feeding a No. 1 anion column to saturation at the flow rate of 90 ~ 100m 1/min;

secondly, eluting with 0.1mol/L hydrochloric acid at the flow rate of 70 ~ 80ml/min, and collecting the eluent by a container;

the third step: and qualitatively grouping multi-bottle eluents by adopting paper chromatography, wherein a developing agent is n-butyl alcohol, glacial acetic acid, absolute ethyl alcohol and water in a volume ratio of =4:1:1:2, and respectively combining components mainly comprising Gly, Ala and Leu and Val.

4. The method according to claim 1 or 2, characterized in that the separation of the acidic amino acids is carried out by:

the first step is to slowly adjust the pH of the acidic amino acid component to 5 ~ 6 with sodium hydroxide, and to saturate the acidic amino acid component by passing through a 717 anion column at a flow rate of 45 ~ 50 ml/min;

secondly, eluting with 0.1mol/L hydrochloric acid at the flow rate of 35 ~ 50ml/min, sequentially collecting eluents by using a container until the ninhydrin reaction is slightly stopped;

the third step: and (3) sequentially carrying out paper chromatography qualitative determination on each bottle of eluent, wherein the developing agent is phenol-water volume ratio =3:1, and combining components with the same Rf value as the standard Asp and Glu.

5. The method according to claim 1 or 2, wherein the method for hydrolyzing the degreased silkworm chrysalis powder comprises the steps of adding 6mol/L hydrochloric acid 15L into 3kg of the degreased silkworm chrysalis powder, uniformly stirring, expanding, slowly heating to 110 ℃ in a glass-lined tank, and carrying out heat preservation hydrolysis for 24 ~ 26 hours.

6. A process as claimed in claim 1 or 2, wherein the deacidification is carried out by cooling the hydrolysate to below 60 ℃ and filtering, washing the filter residue with 5 times of water, combining the filtrate with the washing liquid, and diluting with water to pH 1.5 ~ 2.0.0.

7. The method according to claim 1 or 2, wherein the decolorization method comprises adding 1 ~ 2wt% of activated carbon to the total amount of the deacidified solution, stirring at 90 deg.C for 2h, cooling to 4 ~ 10 deg.C, standing for 1 ~ 2 days, and vacuum filtering to obtain light orange yellow transparent liquid.