阅读说明：本技术 一种利用菲汀制备肌醇的工艺方法 (Process method for preparing inositol by utilizing phytin ) 是由 朱理平 陈科材 何冬生 于 2019-10-21 设计创作，主要内容包括：本发明涉及肌醇技术领域,尤其涉及一种利用菲汀制备肌醇的工艺方法,包括(1)菲汀打浆；(2)水解；(3)石灰乳中和及过滤浓缩；(4)结晶离心；(5)脱色；(6)精制。该工艺方法缩短了菲汀水解的时间,提高了肌醇的收率,同时还可以得到单一副产物,带来了经济效益。(The invention relates to the technical field of inositol, in particular to a process method for preparing inositol by phytin, which comprises the steps of (1) pulping phytin; (2) hydrolyzing; (3) lime milk is neutralized, filtered and concentrated; (4) crystallizing and centrifuging; (5) decoloring; (6) and (5) refining. The process method shortens the hydrolysis time of the phytin, improves the yield of the inositol, and can obtain a single byproduct, thereby bringing economic benefits.)

1. A process method for preparing inositol by utilizing phytin is characterized by comprising the following steps:

(1) pulping by using phytin: pouring the phytin and water into a pulping tank according to a certain proportion, stirring and mixing, and pulping into paste to obtain phytin paste;

(2) hydrolysis: adjusting the pH value of the phytin slurry obtained in the step (1), adding phytase and surfactant, stirring and mixing, controlling the reaction temperature and the reaction time, and obtaining hydrolysate after the reaction is finished;

(3) lime milk neutralization, filtration and concentration: stirring the hydrolysate obtained in the step (2), adding lime milk to adjust the pH value, filtering the neutralized hydrolysate obtained by the reaction through a plate frame, concentrating the collected filtrate to obtain a concentrated solution, and calcining and cooling the collected filter cake;

(4) and (3) crystallizing and centrifuging: cooling and crystallizing the concentrated solution obtained in the step (3), and centrifugally collecting solid which is a crude inositol product;

(5) and (3) decoloring: adding water into the inositol crude product obtained in the step (4) for dissolving, adding activated carbon, heating, stirring and decoloring to obtain a decolored inositol crude product;

(6) refining: crystallizing and centrifuging the inositol crude product decolorized in the step (5), and collecting the solid which is an inositol pure product.

2. The process method for preparing inositol by using phytin as claimed in claim 1, wherein the process comprises the following steps: in the step (1), the phytin and the water are added according to the weight-volume ratio of 1: 2-10.

3. The process method for preparing inositol by using phytin as claimed in claim 1, wherein the process comprises the following steps: in the step (2), the pH value is adjusted to 5.0-5.8, the addition amount of the phytase is 0.1-2% of the weight of the phenanthrene, the addition amount of the surfactant is 0.01-0.05% of the weight of the phenanthrene, the reaction temperature is 60-70 ℃, and the reaction time is 3-12 h.

4. The process method for preparing inositol by using phytin as claimed in claim 3, wherein the process comprises the following steps: the addition amount of the phytase is 0.5-1% of the weight of the phytin.

5. The process method for preparing inositol by using phytin as claimed in claim 1, wherein the process comprises the following steps: the surfactant is tween-80 or polyethylene glycol 400.

6. The process method for preparing inositol by using phytin as claimed in claim 1, wherein the process comprises the following steps: in the step (3), the pH value is adjusted to 9-10, and the calcining temperature of the collected filter cake is 800-1000 ℃.

7. The process method for preparing inositol by using phytin as claimed in claim 1, wherein the process comprises the following steps: in the step (3), the pH value is adjusted to 5-6, and the calcining temperature of the collected filter cake is 70-100 ℃.

8. The process method for preparing inositol by using phytin as claimed in claim 1, wherein the process comprises the following steps: the temperature of cooling crystallization in the step (4) is 20-35 ℃, and the crystallization time is 12-24 h.

9. The process method for preparing inositol by using phytin as claimed in claim 1, wherein the process comprises the following steps: in the step (5), the adding amount of the activated carbon is 0.1-1% of the mass of the inositol crude product, the decoloring temperature is 80-100 ℃, and the decoloring time is 20min-1 h.

Technical Field

The invention relates to the technical field of inositol, in particular to a process method for preparing inositol by phytin.

Background

Inositol, also called inositol, has the formula C₆H₁₂O₆The molecular weight is 180, the product is white crystal powder, the melting point is 224-227 ℃, the product is soluble in water, slightly soluble in ethanol and hardly soluble in diethyl ether, chloroform and anhydrous acetone. Inositol is widely distributed in organisms and is a growth factor of animals and microorganisms. Inositol belongs to vitamin B group, has a plurality of remarkable physiological and pharmacological activities, has better curative effect particularly on mental and neurological diseases, cancers and lipid metabolism diseases, and is widely applied to the fields of food and feed industries to play a good role in promoting the growth and health of people and animals.

At present, the inositol is mainly prepared by hydrolysis of a phytin raw material, and the process method for preparing the inositol by the phytin in the prior art comprises a pressure hydrolysis method, an atmospheric pressure catalytic hydrolysis method and an atmospheric pressure enzymatic reaction method, wherein the pressure hydrolysis method and the atmospheric pressure catalytic hydrolysis method have the problems of expensive production equipment and energy consumption, and the atmospheric pressure enzymatic hydrolysis method has the characteristics of low temperature and low energy consumption, so that more and more production enterprises adopt the method to prepare the inositol. However, there are problems with enzymatic hydrolysis: (1) when the phytase is used for hydrolyzing the phytin, the reaction time is long, and the phytin is not hydrolyzed completely, so that the yield of the inositol is low; (2) and hydrolyzing phytin by phytase to obtain hydrolysate of inositol and phosphoric acid, adding lime milk into the hydrolysate to adjust the pH value to be neutral, filtering by a plate frame, collecting filter residue which is a mixture of calcium phosphate and calcium hydrophosphate, and not utilizing the filter residue as waste. Calcium phosphate is commonly used as an anticaking agent, acidity regulator, nutritional supplement, stabilizer, moisture retention agent, and the like. Calcium hydrogen phosphate can be used as enhancer and swelling agent, and also can be used as feed additive to supplement phosphorus and calcium elements in livestock and poultry feed, promote feed digestion, and increase weight of poultry. The mixture of calcium phosphate and calcium hydrogen phosphate is not convenient to use, and it is not sure to increase the economic efficiency if the above-mentioned by-products of the single components can be obtained simultaneously with the preparation of inositol. However, the prior art does not study and report the side product in the preparation of inositol.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: aiming at the defects of the prior art, the process method for preparing the inositol by using the phytin shortens the hydrolysis time of the phytin, improves the yield of the inositol, and can obtain a single byproduct and bring economic benefits.

In order to solve the technical problems, the technical scheme of the invention is as follows:

a process for preparing inositol by utilizing phytin, which comprises the following steps:

(1) pulping by using phytin: pouring the phytin and water into a pulping tank according to a certain proportion, stirring and mixing, and pulping into paste to obtain phytin paste;

(2) hydrolysis: adjusting the pH value of the phytin slurry obtained in the step (1), adding phytase and surfactant, stirring and mixing, controlling the reaction temperature and the reaction time, and obtaining hydrolysate after the reaction is finished;

(3) lime milk neutralization, filtration and concentration: stirring the hydrolysate obtained in the step (2), adding lime milk to adjust the pH value, filtering the neutralized hydrolysate obtained by the reaction through a plate frame, concentrating the collected filtrate to obtain a concentrated solution, and calcining and cooling the collected filter cake;

(4) and (3) crystallizing and centrifuging: cooling and crystallizing the concentrated solution obtained in the step (3), and centrifugally collecting solid which is a crude inositol product;

(5) and (3) decoloring: adding water into the inositol crude product obtained in the step (4) for dissolving, adding activated carbon, heating, stirring and decoloring to obtain a decolored inositol crude product;

(6) refining: crystallizing and centrifuging the inositol crude product decolorized in the step (5), and collecting the solid which is an inositol pure product.

As an improved technical scheme, the phytin and the water in the step (1) are added according to the weight-volume ratio of 1: 2-10.

As an improved technical scheme, in the step (2), the pH is adjusted to 5.0-5.8, the addition amount of the phytase is 0.1-2% of the weight of the phenanthrene, the addition amount of the surfactant is 0.01-0.05% of the weight of the phenanthrene, the reaction temperature is 60-70 ℃, and the reaction time is 3-12 h.

As a preferable technical scheme, the addition amount of the phytase in the step (2) is 0.5-1% of the weight of the phytin.

As an improved technical scheme, the surfactant is Tween-80 or polyethylene glycol 400.

As an improved technical scheme, the pH value is adjusted to 9-10 in the step (3), and the calcining temperature of the collected filter cake is 800-1000 ℃.

As an improved technical scheme, in the step (3), the pH value is adjusted to 5-6, and the calcining temperature of the collected filter cake is 70-100 ℃.

As an improved technical scheme, the temperature for cooling and crystallizing in the step (4) is 20-35 ℃, and the crystallizing time is 12-24 h.

As an improved technical scheme, the adding amount of the activated carbon in the step (5) is 0.1-1% of the mass of the inositol crude product, the decoloring temperature is 80-100 ℃, and the decoloring time is 20min-1 h.

After the technical scheme is adopted, the invention has the beneficial effects that:

the invention takes the phytin as the raw material, adds water, pulps and adjusts the pH value, then adds the phytase and the surfactant, the addition of the surfactant, namely Tween-80 or polyethylene glycol 400 can promote the full contact between the phytase and the phytin, the phytin hydrolyzation is more thorough, the enzymolysis efficiency of the phytase to the phytin is greatly improved, simultaneously the pH value of a reaction system is controlled to be 5.0-5.8, the reaction temperature is 60-70 ℃, the reaction time is shortened, the yield of the inositol is greatly improved, and the yield of the inositol can reach 97.9%. Neutralizing the hydrolysate with lime milk, regulating pH to 9-10, calcining the collected filter cake at 800-1000 deg.c, and cooling to obtain calcium phosphate as side product; the pH value is adjusted to 5-6, the collected filter cake is calcined at the temperature of 70-100 ℃, and a single-component calcium hydrophosphate byproduct can be obtained after cooling, so that the economic benefit of an enterprise is greatly increased.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.