阅读说明：本技术 一种离心除菌体后色谱提取谷氨酸的工艺 (Process for chromatographic extraction of glutamic acid after centrifugal thallus removal ) 是由 卢松 田永红 梁晓娟 王文强 高雷 王斌 郄子玥 高启超 于 2018-05-31 设计创作，主要内容包括：本发明属于氨基酸分离纯化技术领域,公开了一种离心除菌体后色谱提取谷氨酸的工艺,其包括如下步骤：步骤1)过滤除菌,步骤2)色谱分离,步骤3)树脂柱吸附,步骤4)浓缩结晶。本发明工艺废水产量少,环境压力降低,谷氨酸收率提高,具备较好的工业实用价值。(The invention belongs to the technical field of amino acid separation and purification, and discloses a process for extracting glutamic acid by centrifugation and bacterial body removal and chromatography, which comprises the following steps: step 1) filtering and sterilizing, step 2) chromatographic separation, step 3) resin column adsorption, and step 4) concentrating and crystallizing. The method has the advantages of low process wastewater yield, low environmental pressure, high glutamic acid yield and good industrial practical value.)

1. a process for chromatographic extraction of glutamic acid after centrifugal thallus removal comprises the following steps: step 1) filtering and sterilizing, step 2) chromatographic separation, step 3) resin column adsorption, and step 4) concentrating and crystallizing.

2. the process according to claim 1, characterized in that it comprises the following steps:

Step 1) filtration sterilization: filtering glutamic acid fermentation liquor by using a ceramic membrane to remove mycoprotein, and collecting filtrate;

Step 2) chromatographic separation, namely injecting the filtrate into a sequential simulated moving bed for chromatographic separation at the flow rate of 3-5m ³/h, keeping the temperature of the feed liquid at 70 ℃, collecting the extract after chromatographic separation, then carrying out evaporation concentration by adopting a plate evaporator, concentrating by 2 times, then entering an isoelectric crystallization tank for crystallization, adjusting the pH value to 3.2, and collecting crystals and isoelectric supernatant;

Step 3), resin column adsorption: passing the isoelectric supernatant through a resin column filled with styrene-methyl methacrylate resin, wherein the flow rate of the isoelectric supernatant into the resin column is 2-3 BV/h; the effluent passes through a resin column filled with expandable polystyrene resin again, the flow rate of the effluent entering the column is 1-2BV/h, and the effluent solution is collected;

Step 4), concentration and crystallization: concentrating the solution obtained in the step 3) twice, then performing isoelectric crystallization, collecting crystals, and drying to obtain a glutamic acid product.

3. the process according to claim 2, wherein the ceramic membrane has a pore size of 0.05 to 0.1 μm.

4. the process as claimed in claim 2, wherein the concentration in step 2) is carried out at 70 ℃ under a vacuum of 0.05-0.06 MPa.

5. the process according to claim 2, characterized in that the simulated moving bed chromatographic separation conditions and parameters are: the eluent is pure water, the temperature is kept constant at 70 ℃, the chromatographic column of the simulated moving bed is 8 columns, the different areas of the columns are switched by a double-channel automatic valve, and the resin filled in the columns is hydrophilic resin.

6. the process as claimed in claim 2, wherein the styrene-methyl methacrylate resin has a particle size of 200-300 μm.

7. The process as claimed in claim 2, wherein the particle size of the expandable polystyrene resin is 100-150 μm.

Technical Field

The invention belongs to the technical field of amino acid separation and purification, and particularly relates to a process for extracting glutamic acid by chromatography after centrifugal thallus removal.

Background

Glutamic acid, an acidic amino acid. The molecule contains two carboxyl groups, and the chemical name of the molecule is alpha-aminoglutaric acid. Glutamic acid was found in ryxon 1856 as a colorless crystal, umami-tasting, slightly soluble in water, and soluble in hydrochloric acid solution with an isoelectric point of 3.22. It is abundant in cereal protein, and is also abundant in animal brain. Glutamate plays an important role in protein metabolism in organisms, and is involved in many important chemical reactions in animals, plants, and microorganisms. The monosodium glutamate is prepared from glutamic acid.

the prior art for extracting glutamic acid from glutamic acid fermentation liquor mainly adopts a continuous concentration isoelectric process, the temperature of the concentrated glutamic acid fermentation liquor is controlled to be 42-45 ℃, the glutamic acid fermentation liquor is fed into an isoelectric tank, the pH of the isoelectric tank is controlled to be about 3.2 by sulfuric acid, the glutamic acid is cooled in a cooling tank after crystallization is finished, a centrifugal machine is used for centrifuging the glutamic acid, the centrifuged clear liquid also contains a certain amount of glutamic acid, the content is about 1-2%, the glutamic acid is directly discharged to a sewage treatment plant, the process produces less sewage, an ion exchange process is not needed, and the yield and the quality of the glutamic acid are low. The other method is isoelectric + ion exchange extraction process, which is to cool glutamic acid fermentation liquor, adjust the pH of the fermentation liquor to 3.0-3.2 of isoelectric point of glutamic acid by sulfuric acid and ion exchange high current, standing and settling, centrifugally separate glutamic acid from bottom sediment, adjust the pH of supernatant to 1.8 by sulfuric acid, replace glutamic acid in the supernatant by strong acid cation exchange resin, and adjust the pH of the supernatant to 10 by ammonia water for elution. But the process has the defects of large sewage amount, large acid and alkali consumption and the like. How to extract and purify glutamic acid fermentation to obtain high-quality glutamic acid products is a technical problem to be solved.

Disclosure of Invention

the invention aims to overcome the defects, and provides the process for extracting the glutamic acid by chromatography after centrifugal thallus removal, wherein the process has the advantages of low wastewater yield, reduced environmental pressure, improved glutamic acid yield and better industrial practical value.

The main solution of the invention is realized as follows:

a process for chromatographic extraction of glutamic acid after centrifugal thallus removal comprises the following steps: step 1) filtering and sterilizing, step 2) chromatographic separation, step 3) resin column adsorption, and step 4) concentrating and crystallizing.

Further, the process comprises the following steps:

Step 1) filtration sterilization: filtering glutamic acid fermentation liquor by using a ceramic membrane to remove mycoprotein, and collecting filtrate;

step 2) chromatographic separation, namely injecting the filtrate into a sequential simulated moving bed for chromatographic separation at the average flow velocity of 3-5m ³/h, keeping the temperature of the feed liquid at 70 ℃, collecting the extract after chromatographic separation, then carrying out evaporation concentration by adopting a plate evaporator, concentrating by 2 times, then entering an isoelectric crystallization tank for crystallization, adjusting the pH to 3.2, and collecting crystals and isoelectric supernatant;

step 3), resin column adsorption: passing the isoelectric supernatant through a resin column filled with styrene-methyl methacrylate resin, wherein the flow rate of the isoelectric supernatant into the resin column is 2-3 BV/h; the effluent passes through a resin column filled with expandable polystyrene resin again, the flow rate of the effluent entering the column is 1-2BV/h, and the effluent solution is collected;

step 4), concentration and crystallization: concentrating the solution obtained in the step 3) twice, then performing isoelectric crystallization, collecting crystals, and drying to obtain a glutamic acid product.

Preferably, the first and second electrodes are formed of a metal,

The aperture of the ceramic membrane is 0.05-0.1 μm.

preferably, the first and second electrodes are formed of a metal,

In the step 2), the concentration temperature is 70 ℃, and the vacuum degree is 0.05-0.06 MPa.

Preferably, the first and second electrodes are formed of a metal,

The simulated moving bed chromatographic separation conditions and parameters are as follows: the eluent is pure water, the temperature is kept constant at 70 ℃, the chromatographic column of the simulated moving bed is 8 columns, the different areas of the columns are switched by a double-channel automatic valve, and the resin filled in the columns is hydrophilic resin.

preferably, the first and second electrodes are formed of a metal,

The particle size of the styrene-methyl methacrylate resin is 200-300 microns.

preferably, the first and second electrodes are formed of a metal,

the particle size of the expandable polystyrene resin is 100-150 microns.

Compared with the prior art, the technology of the invention adopts the process, and has the advantages that:

Compared with the traditional ion exchange process, the simulated moving bed chromatography process for extracting and purifying the glutamic acid does not consume sulfuric acid and liquid ammonia, greatly reduces the amount of sodium hydroxide and water, not only promotes water conservation and reduces cost, but also reduces the difficulty of subsequent sewage treatment and reduces pollution;

Because the contents of pigments, heteropolyacids and small molecular proteins in the fermentation liquor are high, the contents of pigments, heteropolyacids and small molecular proteins cannot be completely removed by a simulated moving bed chromatography process, so that the isoelectric supernatant still contains the impurities, the isoelectric supernatant obtained by isoelectric extraction of glutamic acid crystals is extracted and purified, the waste of the supernatant is avoided, the yield of the glutamic acid reaches more than 80%, and the purity reaches more than 98%;

The method adopts two times of adsorption column treatment, so that components such as pigments, saccharides and the like are removed for the first time, and components such as small molecular polypeptide protein and the like are removed for the second time, so that impurities are greatly reduced; the adsorption resin is different from ion resin, does not contain ion exchange groups, is relatively easy to regenerate, does not need to consume a large amount of acid, alkali and water resources, and can be repeatedly used;

the invention has simple and feasible operation process, less wastewater output, reduced environmental pressure and better industrial practical value.

Detailed Description

in order to make those skilled in the art better understand the technical solutions in the present application, the technical solutions in the present application will be clearly and completely described below with reference to specific embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.