阅读说明：本技术 一种抗穆勒氏管激素（amh）重组蛋白与其单克隆抗体制备用培养皿及使用方法 (Culture dish for anti-mullerian hormone (AMH) recombinant protein and monoclonal antibody thereof and using method ) 是由 肖潇 童坤 于 2019-08-28 设计创作，主要内容包括：本发明公开了一种抗穆勒氏管激素(AMH)重组蛋白与其单克隆抗体制备用培养皿及使用方法,包括底座,底座的上方设置有外壳,外壳的底部两侧与底座的顶部两侧均开设有螺纹孔,螺纹孔内螺纹连接有螺栓,外壳的顶部中心固定连接有螺纹管,螺纹管内螺纹连接有螺纹杆,螺纹杆顶端固定连接有摇把,其下方设置有升降架,升降架顶部固定连接有传动架,螺纹杆的底端贯穿传动架并固定连接有传动块,升降架的顶部两侧均固定连接有第一弹簧,升降架底部固定连接有压块,压块的正下方设置有基座,基座底部固定连接有若干根支撑杆,基座上放置有皿身,皿身上方设置有皿盖。本发明具有结构设计合理,功能多样,并且密闭性和稳定性好等特点。(The invention discloses a culture dish for preparing anti-mullerian hormone (AMH) recombinant protein and monoclonal antibody thereof and a using method thereof, the culture dish comprises a base, a shell is arranged above the base, threaded holes are respectively arranged on two sides of the bottom of the shell and two sides of the top of the base, a bolt is connected in the threaded hole, a threaded pipe is fixedly connected in the center of the top of the shell, a threaded rod is connected in the threaded pipe, a crank is fixedly connected at the top end of the threaded rod, the lifting frame is arranged below the lifting frame, the transmission frame is fixedly connected to the top of the lifting frame, the bottom end of the threaded rod penetrates through the transmission frame and the transmission block, the first springs are fixedly connected to the two sides of the top of the lifting frame, the pressing block is fixedly connected to the bottom of the lifting frame, the base is arranged below the pressing block, the support rods are fixedly connected to the bottom of the base, the dish body is placed on the base, and the dish cover is arranged above the dish body. The invention has the characteristics of reasonable structural design, multiple functions, good tightness and stability and the like.)

1. a culture dish for preparing anti-mullerian hormone (AMH) recombinant protein and monoclonal antibody thereof comprises a base (1) and is characterized in that: the lifting frame is characterized in that a shell (2) is arranged above the base (1), threaded holes (3) are formed in two sides of the bottom of the shell (2) and two sides of the top of the base (1), bolts (4) are connected with the threaded holes (3) in an internal thread manner, handles (5) are fixedly connected to the outer walls of the top ends of two sides of the shell (2), a threaded pipe (6) is fixedly connected to the center of the top of the shell, a threaded rod (7) is connected with the threaded pipe (6) in an internal thread manner, a crank (8) is fixedly connected to the top end of the threaded rod (7), a lifting frame (9) is arranged below the crank, a transmission frame (10) is fixedly connected to the top of the lifting frame (9), the bottom end of the threaded rod (7) penetrates through the transmission frame (10) and is fixedly connected with a transmission block (11), first springs (12) are fixedly connected to two sides of the, the bottom fixedly connected with briquetting (13) of crane (9), be provided with base (14) under briquetting (13), a plurality of spinal branch vaulting poles (15) of bottom fixedly connected with of base (14), the bottom fixed connection of bracing piece (15) is on base (1), dish body (16) have been placed on base (14), the top of dish body (16) is provided with dish lid (17).

2. the culture dish for preparing an anti-Mullerian hormone (AMH) recombinant protein and a monoclonal antibody thereof according to claim 1, wherein: the top lateral wall fixedly connected with a plurality of fixed block (18) of ware body (16), every the equal fixedly connected with reference column (19) in top of fixed block (18), a plurality of locating hole (20) have been seted up to the lateral wall of ware lid (17), the quantity of locating hole (20) is the same with reference column (19), and its aperture equals the diameter of reference column (19), just the center of locating hole (20) and reference column (19) is located same straight line.

3. the culture dish for preparing an anti-Mullerian hormone (AMH) recombinant protein and a monoclonal antibody thereof according to claim 1, wherein: the bottom surface fixed connection of ware body (16) has a round fixture block (21), the top surface of base (14) is seted up with fixture block (21) matched with draw-in groove (22), draw-in groove (22) are circular groove structure.

4. The culture dish for preparing an anti-Mullerian hormone (AMH) recombinant protein and a monoclonal antibody thereof according to claim 1, wherein: the utility model discloses a dish ware, including base (14), the both sides outer wall fixedly connected with mount (23) of base (14), the through-hole has been seted up on mount (23), and pull rod (24) have been inserted in the through-hole, the one end fixedly connected with pull ring (25) of pull rod (24), its other end fixedly connected with clamp splice (26), just the pole body that pull rod (24) are close to ware body (16) one end is gone up the winding and is had second spring (27), the both ends difference fixed connection of second spring (27) is on mount (23) and clamp splice (26).

5. The culture dish for preparing an anti-Mullerian hormone (AMH) recombinant protein and a monoclonal antibody thereof according to claim 4, wherein: the clamping block (26) is arc-shaped, a layer of rubber gasket is arranged on the surface of the inner wall of the clamping block, and herringbone anti-skidding lines are carved on the surface of the rubber gasket.

6. The culture dish for preparing an anti-Mullerian hormone (AMH) recombinant protein and a monoclonal antibody thereof according to claim 1, wherein: the bottom of the pressing block (13) is provided with a groove, the groove width of the pressing block is equal to the width of the dish cover (17), and a plurality of hemispherical rubber blocks are uniformly distributed on the surface of the groove in the bottom of the pressing block (13).

7. The culture dish for preparing an anti-Mullerian hormone (AMH) recombinant protein and a monoclonal antibody thereof according to claim 1, wherein: the lifting frame is characterized in that wheel frames (28) are fixedly connected to the side walls of the two sides of the lifting frame (9), guide wheels (29) are installed on the wheel frames (28), a circle of wheel teeth are uniformly distributed on the surface of the wheel wall of each guide wheel (29), and a plurality of strip teeth meshed with the wheel teeth of each guide wheel (29) are uniformly distributed on the surface of the inner wall of the two sides of the shell (2).

8. the culture dish for preparing an anti-Mullerian hormone (AMH) recombinant protein and a monoclonal antibody thereof according to claim 1, wherein: the bottom surface of the shell (2) is provided with a layer of sealing gasket (30).

9. The use of the culture dish for the preparation of recombinant protein Against Mullerian Hormone (AMH) and its monoclonal antibody according to claims 1-8, comprising the following steps:

S1: placing the dish body (16) with the AMH recombinant protein and the monoclonal antibody sample thereof on the base (14), aligning the clamping blocks (21) at the bottom of the dish body and inserting the clamping blocks into the clamping grooves (22) on the surface of the base (14), pulling the pull ring (25) to drive the pull rod (24) to move, and tightly pressing the clamping blocks (26) against the side wall surface of the dish body (16) under the resetting action of the second spring (27), thereby positioning the dish body (16) on the base (14);

S2: covering the dish cover (17) on the dish body (16), and enabling each positioning column (19) at the top of the fixed block (18) to be respectively aligned with and inserted into the corresponding positioning hole (20), so that the dish cover (17) is positioned on the dish body (16);

S3: placing the shell (2) on the base (1), screwing the bolt (4) in the threaded hole (3), and fixing the shell (2) on the base (1) by using the bolt (4);

S4: the crank handle (8) is held and rotated to drive the threaded rod (7) to move downwards along the threaded pipe (6), the transmission block (11) at the tail end of the threaded rod (7) can drive the transmission frame (10) to move downwards together with the lifting frame (9) when the threaded rod (7) moves downwards until the pressing block (13) at the bottom of the lifting frame (9) is contacted with the dish cover (17), and therefore the dish cover (17) is tightly propped against the dish body (16) by utilizing the downward pressure of the pressing block (13).

Technical Field

The invention relates to the technical field of cell culture, in particular to a culture dish for preparing an anti-mullerian hormone (AMH) recombinant protein and a monoclonal antibody thereof and a using method.

Background

Anti-mullerian hormone, also known as AMH, is a dimeric glycoprotein of 144kDa of the transforming growth factor (TGF- β) family, which includes many factors that act on growth and differentiation. AMH is a dimeric prohormone, which consists of two identical subunits linked by disulfide bonds. The AMH recombinant protein is a protein obtained by applying the technology of AMH recombinant DNA or recombinant RNA, whereas a monoclonal antibody is a highly homogeneous antibody directed only to a certain epitope produced by a single B cell clone, and the preparation of AMH recombinant protein and its monoclonal antibody can help to select the optimal strategy for a patient by specifically optimizing the steps of controlled stimulation of the ovaries while avoiding the risk of overstimulation, and also enables monitoring of changes in ovarian reserves in young girls or women already receiving gonadotropin therapy, for example in the case of cancer.

Preparation work of AMH recombinant protein and its monoclonal antibody all needs to go on in the culture dish usually, and the most simple structure of current culture dish, the function is single, the seal is poor, the air enters into the culture dish easily and contacts with inside AMH recombinant protein and monoclonal antibody among the culture process, thereby make the sample receive the pollution, and the poor stability of culture dish, take place to rock because of exogenic action easily in the preparation process, thereby influence the normal culture of AMH recombinant protein and monoclonal antibody, consequently, need to improve it urgently.

Disclosure of Invention

The invention aims to overcome the defects of simple structure, single function and poor tightness and stability of a culture dish in the prior art, and provides a culture dish for preparing an anti-mullerian hormone (AMH) recombinant protein and a monoclonal antibody thereof and a using method thereof. The culture dish for preparing the anti-mullerian hormone (AMH) recombinant protein and the monoclonal antibody thereof and the using method have the characteristics of reasonable structural design, multiple functions, good tightness and stability and the like.

In order to achieve the purpose, the invention provides the following technical scheme: a culture dish for preparing anti-mullerian hormone (AMH) recombinant protein and monoclonal antibody thereof comprises a base, wherein a shell is arranged above the base, threaded holes are formed in two sides of the bottom of the shell and two sides of the top of the base, bolts are connected with the threaded holes in a threaded manner, handles are fixedly connected to the outer walls of the top ends of the two sides of the shell, a threaded pipe is fixedly connected to the center of the top of the shell, a threaded rod is connected with the threaded pipe in a threaded manner, a crank is fixedly connected to the top end of the threaded rod, a lifting frame is arranged below the threaded pipe, a transmission frame is fixedly connected to the top of the lifting frame, a transmission block is fixedly connected to the bottom end of the threaded rod in a penetrating manner, first springs are fixedly connected to two sides of the top of the lifting frame, the top ends of the, the dish is characterized in that a base is arranged under the pressing block, a plurality of supporting rods are fixedly connected to the bottom of the base, the bottom ends of the supporting rods are fixedly connected to the base, a dish body is placed on the base, and a dish cover is arranged above the dish body.

Preferably, the top lateral wall fixedly connected with a plurality of fixed block of ware body, every the equal fixedly connected with reference column in top of fixed block, a plurality of locating hole has been seted up to the lateral wall of ware lid, the quantity of locating hole is the same with the reference column, and its aperture equals the diameter of reference column, just the locating hole is located same straight line with the center of reference column.

Preferably, the bottom surface of the dish body is fixedly connected with a circle of clamping blocks, the top surface of the base is provided with clamping grooves matched with the clamping blocks, and the clamping grooves are of circular groove structures.

Preferably, the outer wall fixedly connected with mount of both sides of base, the through-hole has been seted up on the mount, and the pull rod has been inserted in the through-hole, the one end fixedly connected with pull ring of pull rod, its other end fixedly connected with clamp splice, just the pole body that the pull rod is close to ware body one end twines has the second spring, the both ends of second spring fixed connection respectively are on mount and clamp splice.

preferably, the clamping block is arc-shaped, a layer of rubber gasket is arranged on the surface of the inner wall of the clamping block, and herringbone anti-skidding lines are carved on the surface of the rubber gasket.

Preferably, the bottom of the pressing block is provided with a groove, the groove width of the pressing block is equal to the width of the dish cover, and a plurality of hemispherical rubber blocks are uniformly distributed on the surface of the groove at the bottom of the pressing block.

Preferably, the side walls of the two sides of the lifting frame are fixedly connected with wheel frames, the wheel frames are provided with guide wheels, a circle of wheel teeth are uniformly distributed on the surface of the wheel wall of each guide wheel, and a plurality of rack teeth meshed with the wheel teeth of each guide wheel are uniformly distributed on the surface of the inner wall of the two sides of the shell.

Preferably, the bottom surface of the housing is provided with a layer of gasket.

Preferably, the method for using the culture dish for preparing the anti-mullerian hormone (AMH) recombinant protein and the monoclonal antibody thereof comprises the following steps:

S1: placing the dish body with the AMH recombinant protein and the monoclonal antibody sample thereof on a base, aligning a clamping block at the bottom of the dish body with a clamping groove on the surface of the base, pulling the pull ring to drive the pull ring to move, and tightly pressing the clamping block against the surface of the side wall of the dish body under the reset action of a second spring so as to position the dish body on the base;

S2: covering the dish cover on the dish body, and enabling the positioning columns at the top of the fixing block to be respectively aligned and inserted into the corresponding positioning holes, so that the dish cover is positioned on the dish body;

s3: placing the shell on a base, screwing the bolt in the threaded hole, and fixing the shell on the base by using the bolt;

S4: holding and rotating the crank and making it drive the threaded rod to move down along the threaded pipe, the transmission block at the tail end of the threaded rod can drive the transmission frame to move down together with the lifting frame when the threaded rod moves down until the pressing block at the bottom of the lifting frame is contacted with the dish cover, thereby utilizing the downward pressure of the pressing block to tightly support the dish cover on the dish body.

Compared with the prior art, the invention has the beneficial effects that:

The culture dish for preparing the anti-mullerian hormone (AMH) recombinant protein and the monoclonal antibody thereof has reasonable structural design and multiple functions, can fill a gap between the shell and the base by using the sealing gasket, so as to prevent air from entering the shell from the gap to cause sample pollution in the culture process, can improve the stability of the dish body by using the mutual matching between the clamping block and the clamping groove so as to prevent the dish body from shaking under the action of external force, can further improve the stability of the dish body by using the clamping block so as to prevent the dish body from displacing, can position the dish cover on the dish body by using the mutual matching between the positioning column and the positioning hole so as to prevent the dish cover from displacing, and can press the dish cover on the dish body 16 by using the pressing block so as to cover the dish cover on the dish body, so as to prevent the gap from being generated between the dish cover and the dish body.

drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is an enlarged view of FIG. 1 at A;

Fig. 3 is a schematic perspective view of the clamping block.

Reference numbers in the figures: 1. a base; 2. a housing; 3. a threaded hole; 4. a bolt; 5. a handle; 6. a threaded pipe; 7. a threaded rod; 8. a crank; 9. a lifting frame; 10. a transmission frame; 11. a transmission block; 12. a first spring; 13. briquetting; 14. a base; 15. a support bar; 16. a dish body; 17. a dish cover; 18. a fixed block; 19. a positioning column; 20. positioning holes; 21. a clamping block; 22. a card slot; 23. a fixed mount; 24. a pull rod; 25. a pull ring; 26. a clamping block; 27. a second spring; 28. a wheel carrier; 29. a guide wheel; 30. and a gasket.

Detailed Description

the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-3, the present invention provides a technical solution: a culture dish for preparing anti-Mullerian hormone (AMH) recombinant protein and monoclonal antibody thereof and a using method thereof comprise a base 1, a shell 2 is arranged above the base 1, a layer of sealing gasket 30 is arranged on the bottom surface of the shell 2, the sealing gasket 30 is made of rubber and has elasticity, and can fill a gap between the shell 2 and the base 1 so as to prevent air from entering the shell 2 from the gap to cause sample pollution in the culture process, threaded holes 3 are respectively arranged on two sides of the bottom of the shell 2 and two sides of the top of the base 1, bolts 4 are connected in the threaded holes 3, handles 5 are respectively fixedly connected on the outer walls of the top ends of two sides of the shell 2, a threaded pipe 6 is fixedly connected in the center of the top of the shell, a threaded rod 7 is connected in the threaded pipe 6, a crank 8 is fixedly connected at the top end of the threaded rod 7, a lifting frame 9 is arranged, the wheel carrier 28 is provided with a guide wheel 29, the surface of the wheel wall of the guide wheel 29 is uniformly distributed with a circle of wheel teeth, the surface of the inner wall of the two sides of the shell 2 is uniformly distributed with a plurality of strip teeth meshed with the wheel teeth of the guide wheel 29, the stability of the lifting frame 9 can be improved by utilizing the mutual meshing between the strip teeth and the wheel teeth, the lifting frame 9 is prevented from shaking and inclining when moving up and down, the top of the lifting frame 9 is fixedly connected with a transmission frame 10, the bottom end of a threaded rod 7 penetrates through the transmission frame 10 and is fixedly connected with a transmission block 11, the two sides of the top of the lifting frame 9 are both fixedly connected with a first spring 12, the top end of the first spring 12 is fixedly connected with the top of the shell 2, the bottom of the lifting frame 9 is fixedly connected with a pressing block 13, a base 14 is arranged under the pressing block 13, a dish cover 17 is arranged above the dish body 16, a groove is formed at the bottom of the pressing block 13, the groove width is equal to the width of the dish cover 17, the pressing block 13 can be used for pressing down the dish cover 17 so as to cover the dish cover 17 on the dish body 16, a plurality of hemispherical rubber blocks are uniformly distributed on the surface of the groove at the bottom of the pressing block 13, the rubber blocks have elasticity and can be elastically deformed when being extruded, so that the buffer protection effect can be achieved, the dish cover 17 is prevented from being damaged due to overlarge pressing force of the pressing block 13, a plurality of fixed blocks 18 are fixedly connected with the side wall at the top end of the dish body 16, a positioning column 19 is fixedly connected with the top of each fixed block 18, a plurality of positioning holes 20 are formed in the side wall of the dish cover 17, the number of the positioning holes 20 is the same as that of the positioning columns 19, the aperture is equal to the diameter of the positioning columns 19, the centers of the positioning holes 20 and the positioning columns 19 are located on the, in order to avoid displacement, a circle of clamping blocks 21 are fixedly connected to the bottom surface of the dish body 16, a clamping groove 22 matched with the clamping blocks 21 is formed in the top surface of the base 14, the clamping groove 22 is of a circular groove structure, the stability of the dish body 16 can be improved by utilizing the mutual matching between the clamping blocks 21 and the clamping groove 22 so as to prevent the dish body 16 from shaking under the action of external force, fixing frames 23 are fixedly connected to the outer walls of the two sides of the base 14, through holes are formed in the fixing frames 23, pull rods 24 are inserted into the through holes, pull rings 25 are fixedly connected to one ends of the pull rods 24, clamping blocks 26 are fixedly connected to the other ends of the pull rods 24, a second spring 27 is wound on a rod body close to one end of the dish body 16, the two ends of the second spring 27 are respectively and fixedly connected to the fixing frames 23 and the clamping blocks 26, the clamping blocks 26 are arc-shaped and can be attached to the side wall surface of, the friction between clamp blocks 26 and dish body 16 may be increased to further improve the stability of dish body 16 and prevent displacement thereof.

The working principle is as follows: when the invention is used, firstly, the dish body 16 containing the AMH recombinant protein and the monoclonal antibody sample thereof is placed on the base 14, the fixture blocks 21 at the bottom of the dish body are aligned with and inserted into the fixture grooves 22 on the surface of the base 14, then the pull ring 25 is pulled to drive the pull rod 24 to move, the clamp blocks 26 are tightly pressed against the side wall surface of the dish body 16 under the reset action of the second spring 27, so that the dish body 16 is positioned on the base 14, then the dish cover 17 is covered on the dish body 16, and the positioning columns 19 at the top of the fixing blocks 18 are respectively aligned with and inserted into the corresponding positioning holes 20, so that the dish cover 17 is positioned on the dish body 16, then the shell 2 is placed on the base 1, the bolts 4 in the threaded holes 3 are screwed, the shell 2 is fixed on the base 1 by using the bolts 4, then the crank 8 is held and rotated to drive the threaded rod 7 to move downwards along the threaded pipe 6, when the threaded rod 7 moves downwards, the transmission block 11 at the tail end of the threaded rod drives the transmission frame 10 to move downwards together with the lifting frame 9 until the pressing block 13 at the bottom of the lifting frame 9 is contacted with the dish cover 17, so that the dish cover 17 is tightly propped against the dish body 16 by using the downward pressure of the pressing block 13.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.