阅读说明：本技术 一种光场光学显微镜及其光场显微成像分析系统 (Light field optical microscope and light field microscopic imaging analysis system thereof ) 是由 李莉华 李荣彬 王孝忠 陈建良 于 2018-05-30 设计创作，主要内容包括：一种光场光学显微镜及其光场显微成像分析系统,包括光学显微镜,多向照明系统,安装在所述光学显微镜中,用于为光场图像的拍摄和视频记录提供多向光源和可控的光通量；光场相机,安装在所述光学显微镜的顶部,用于捕获光场图像或记录光场视频。该光场显微成像分析系统,包括：光场光学显微镜和图像分析处理系统,图像分析处理系统与所述光场相机相连,用于获取所述光场相机捕获的光场图像和视频,分析和处理所述光场图像,以生成三维立体图或重聚焦图像。本发明所提供的光场光学显微镜及其光场显微成像分析系统结构简单、成本低廉,操作方便,能够进行浅景深的小焦点区域的样品观察且能够准确地捕获光场图像,以生成三维立体图或三维视频。(A light field optical microscope and a light field microscopic imaging analysis system thereof comprise an optical microscope, a multidirectional lighting system, a light source and a light source control system, wherein the multidirectional lighting system is arranged in the optical microscope and is used for providing a multidirectional light source and controllable luminous flux for shooting and video recording of a light field image; a light field camera mounted on top of the optical microscope for capturing light field images or recording light field video. The light field microscopic imaging analysis system comprises: the system comprises a light field optical microscope and an image analysis and processing system, wherein the image analysis and processing system is connected with the light field camera and is used for acquiring light field images and videos captured by the light field camera, analyzing and processing the light field images and generating a three-dimensional stereogram or a refocusing image. The light field optical microscope and the light field microscopic imaging analysis system thereof provided by the invention have the advantages of simple structure, low cost and convenient operation, can be used for observing a sample in a small focus area with shallow depth of field and accurately capturing a light field image so as to generate a three-dimensional stereo image or a three-dimensional video.)

1. A light field optical microscope, comprising an optical microscope (110), characterized in that it further comprises: a multi-directional illumination system (120) mounted in the optical microscope (110) for providing a multi-directional light source and controllable light flux for capturing and video recording of light field images;

A light field camera (130) mounted on top of the optical microscope (110) for capturing light field images or recording light field video.

2. the light field optical microscope as defined in claim 1, wherein the light field camera (130) comprises: a digital camera (131) and a compound eye piece (132) installed in the digital camera (131), the digital camera (131) including a cover glass and an image sensor (1311) at one side of the cover glass, the compound eye piece (132) being installed at a side between the cover glass and the image sensor (1311) near the image sensor (1311).

3. the light field optical microscope as defined in claim 2, wherein the fly eye element (132) is a microlens array that is injection molded in a precision molding machine after being ultra-precision machined.

4. The light-field optical microscope as claimed in claim 1, characterized in that the optical microscope (110) comprises a holder (111), an object stage (112) mounted on the holder (111), and a sample placement for placing a sample (113) is provided on a side of the object stage (112) facing away from the holder (111);

The multi-directional illumination system (120) comprises a backlighting system (121) and an ambient illumination system (122), the backlighting system (121) being arranged on a side of the mirror mount (111) facing the object stage (112); the ambient lighting system (122) is arranged on the side of the stage (112) facing away from the lens holder (111) and around the sample placement site, and is used for providing a circumferential multi-directional light source and controllable luminous flux for shooting of light field images and recording of light field videos.

5. The light-field optical microscope as claimed in claim 4, characterized in that the ambient illumination system (122) comprises a display ring (1221), a plurality of LED lamps (1222) and a plurality of switches in one-to-one correspondence with the plurality of LED lamps (1222), the display ring (1221) being arranged on a side of the object stage (112) facing away from the lens holder (111) and being arranged around the sample placement site, the plurality of LED lamps (1222) being distributed uniformly over a ring wall of the display ring (1221), the plurality of switches being arranged on a ring wall of the display ring (1221) in one-to-one correspondence with the plurality of LED lamps (1222).

6. The light field optical microscope as claimed in claim 1, characterized in that the optical microscope (110) further comprises a tube (114), the tube (114) having opposite ends, one end being an objective lens (115) and the other end being a top of the optical microscope for mounting the optical camera (130).

7. The light-field optical microscope as claimed in claim 6, characterized in that one side of the lens tube (114) extends beyond the eyepiece tube for the arrangement of an eyepiece (117); a semi-transparent lens (116) is arranged at the communication part of the eyepiece tube and the eyepiece tube (114) and is used for changing the light transmitted from the objective lens (115).

8. The light field optical microscope as defined in claim 1, further comprising a correction lens (140) mounted between the optical microscope (110) and the light field camera (130) for matching the optical microscope (110) and the light field camera (130) and correcting the light path.

9. A light field microscopic imaging analysis system, comprising: the light field optical microscope (100) of claims 1-8;

An image analysis processing system (200) connected to the light field camera (130) for acquiring light field images and video captured by the light field camera (130), analyzing and processing the light field images to generate a three-dimensional stereogram or refocus image.

10. The light field microscopy imaging analysis system according to claim 9, characterized in that the image analysis processing system (200) comprises:

An image receiving module (210) connected to the light field camera (130) for acquiring the light field image and the video captured by the light field camera (130);

And the analysis processing module (220) is connected with the image receiving module (210), extracts the pixels of the light field image and the video, and divides the pixels into a plurality of pixel groups according to the focal depth information of the pixels.

and the image generation module (230) is connected with the analysis processing module (220) and generates a three-dimensional stereogram or a refocused image according to the pixel group.