阅读说明：本技术 一种灵芝灰树花降血糖组合物及其制备方法和用途 (Ganoderma lucidum and grifola frondosa hypoglycemic composition as well as preparation method and application thereof ) 是由 焦春伟 谢意珍 马晓伟 谭许朋 何春艳 李舜贤 梁慧嘉 陈家明 于 2019-10-11 设计创作，主要内容包括：本发明涉及一种组合物,尤其是一种具有降血糖功效的以灵芝、灰树花、苦瓜为主要原料的组合物及其制备方法和用途,组合物包括以下重量百分比的原料制备得到：灵芝20-40％、灰树花40-60％、苦瓜10-30％。本发明的有益效果：(1)具有天然、安全的特点,同时食用菌与天然降血糖物质复配,充分发挥各原料在降血糖方面的功效,具有良好的降血糖协调作用。(2)制备方法中采用的微滤和超滤截留技术具有操作简单,能耗低,生产周期短等特点,最大限度分离和保护灰树花提取液中活性成分,去除了功效性较差的成分。(3)经过动物实验研究表明,降血糖效果显著。(The invention relates to a composition, in particular to a composition which has the effect of reducing blood sugar and takes ganoderma lucidum, grifola frondosa and bitter gourd as main raw materials, a preparation method and application thereof, wherein the composition is prepared from the following raw materials in percentage by weight: 20-40% of lucid ganoderma, 40-60% of grifola frondosa and 10-30% of bitter gourd. The invention has the beneficial effects that: (1) the edible fungi and the natural hypoglycemic substances are compounded, so that the effects of the raw materials in the aspect of reducing blood sugar are fully exerted, and the edible fungi has a good coordination effect of reducing blood sugar. (2) The microfiltration and ultrafiltration interception technologies adopted in the preparation method have the characteristics of simple operation, low energy consumption, short production period and the like, and can separate and protect active ingredients in the grifola frondosa extract to the maximum extent and remove ingredients with poor efficacy. (3) Animal experiment research shows that the hypoglycemic agent has obvious hypoglycemic effect.)

1. The composition for reducing blood sugar of ganoderma lucidum and grifola frondosa is characterized by being prepared from the following raw materials in percentage by weight: 20-40% of lucid ganoderma, 40-60% of grifola frondosa and 10-30% of bitter gourd.

2. The composition as claimed in claim 1, wherein the grifola frondosa in the composition is treated by the following method: taking a grifola frondosa raw material, adding 8-20 times of water by weight, heating to 80-105 ℃, extracting for 1-3 times, wherein the single extraction time is 1-4 hours, filtering the extracting solutions, combining, concentrating until the solid content is 5% -20%, obtaining a grifola frondosa extract concentrated solution, performing microfiltration treatment on the grifola frondosa extract concentrated solution, collecting microfiltration permeating solution, performing ultrafiltration on the microfiltration permeating solution, performing interception purification on the extracted components by using an ultrafiltration membrane, wherein the pore size of the ultrafiltration membrane corresponds to the molecular weight intercepted by 1-10KDa, the operating pressure is 3-8MPa, the temperature is 25-40 ℃, retaining ultrafiltration intercepting solution which does not permeate through the membrane, obtaining grifola frondosa extract ultrafiltration intercepting solution, and drying the intercepting solution to obtain the grifola frondosa extract.

3. The composition according to claim 1, wherein the ganoderma lucidum in the composition is treated by the following method: adding 8-40 times of water into Ganoderma lucidum raw material, extracting at 80-105 deg.C for 1-3 times, each time for 2-6 hr, filtering the extractive solutions, mixing, concentrating to solid content of 5% -25% to obtain Ganoderma lucidum extract concentrate, and drying the Ganoderma lucidum extract concentrate to obtain Ganoderma lucidum extract.

4. A composition according to claim 1 wherein the goya in the composition is treated by a method comprising: taking a bitter gourd raw material, adding 8-20 times of water by weight, extracting for 1-3 times at 80-105 ℃, wherein the extraction time is 1-4 hours each time, filtering the extracting solution, combining, concentrating to the solid content of 5% -25% to obtain a bitter gourd extract concentrated solution, and drying the bitter gourd extract concentrated solution to obtain the bitter gourd extract.

5. the composition according to claim 1, wherein the ganoderma lucidum and momordica charantia in the composition are treated by the method comprising: mixing Ganoderma and fructus Momordicae Charantiae, adding 8-40 times of water, extracting at 80-105 deg.C for 1-3 times, each for 1-4 hr, filtering the extractive solutions, mixing, concentrating to solid content of 5% -25% to obtain Ganoderma and fructus Momordicae Charantiae extract concentrate, and drying to obtain Ganoderma and fructus Momordicae Charantiae extract.

6. A preparation method of a ganoderma lucidum and grifola frondosa hypoglycemic composition is characterized by comprising the following steps:

(1) Preparing a grifola frondosa extract: taking a grifola frondosa raw material, adding 8-20 times of water by weight, heating to 80-105 ℃, extracting for 1-3 times, wherein the single extraction time is 1-4 hours, filtering the extracting solutions, combining, concentrating until the solid content is 5% -20%, obtaining a grifola frondosa extract concentrated solution, performing microfiltration treatment on the grifola frondosa extract concentrated solution, collecting microfiltration permeating solution, performing ultrafiltration on the microfiltration permeating solution, performing interception purification on the extracted components by using an ultrafiltration membrane, wherein the pore size of the ultrafiltration membrane corresponds to the molecular weight intercepted by 1-10KDa, the operating pressure is 3-8MPa, the temperature is 25-40 ℃, retaining ultrafiltration intercepting solution which does not permeate through the membrane, obtaining grifola frondosa extract ultrafiltration intercepting solution, and drying the intercepting solution to obtain a grifola frondosa extract;

(2) The ganoderma lucidum and bitter gourd extract: mixing a ganoderma lucidum raw material and a bitter gourd raw material, adding 8-40 times of water by weight, extracting for 1-3 times at 80-105 ℃, wherein the extraction time is 1-4 hours each time, filtering the extracting solution, combining, concentrating until the solid content is 5% -25% to obtain a ganoderma lucidum bitter gourd extract concentrated solution, and drying the ganoderma lucidum bitter gourd extract concentrated solution to obtain a ganoderma lucidum bitter gourd extract;

(3) Mixing the extract of Ganoderma and fructus Momordicae Charantiae with the extract of Grifola frondosa to obtain composition;

Wherein the weight percentages of the prepared raw materials are as follows: 20-40% of lucid ganoderma, 40-60% of grifola frondosa and 10-30% of bitter gourd.

7. A preparation method of a ganoderma lucidum and grifola frondosa hypoglycemic composition is characterized by comprising the following steps:

(1) Preparing a ganoderma lucidum extract: adding 8-40 times of water into Ganoderma lucidum raw material, extracting at 80-105 deg.C for 1-3 times, each time for 2-6 hr, filtering the extractive solutions, mixing, concentrating to solid content of 5% -25% to obtain Ganoderma lucidum extract concentrate, and drying the Ganoderma lucidum extract concentrate to obtain Ganoderma lucidum extract;

(2) Preparing a bitter gourd extract: taking a bitter gourd raw material, adding 8-20 times of water by weight, extracting for 1-3 times at 80-105 ℃, wherein the extraction time is 1-4 hours each time, filtering the extracting solution, combining, concentrating to the solid content of 5% -25% to obtain a bitter gourd extract concentrated solution, and drying the bitter gourd extract concentrated solution to obtain a bitter gourd extract;

(3) Preparing a grifola frondosa extract: taking a grifola frondosa raw material, adding 8-20 times of water by weight, heating to 80-105 ℃, extracting for 1-3 times, wherein the single extraction time is 1-4 hours, filtering the extracting solutions, combining, concentrating until the solid content is 5% -20%, obtaining a grifola frondosa extract concentrated solution, performing microfiltration treatment on the grifola frondosa extract concentrated solution, collecting microfiltration permeating solution, performing ultrafiltration on the microfiltration permeating solution, performing interception purification on the extracted components by using an ultrafiltration membrane, wherein the pore size of the ultrafiltration membrane corresponds to the molecular weight intercepted by 1-10KDa, the operating pressure is 3-8MPa, the temperature is 25-40 ℃, retaining ultrafiltration intercepting solution which does not permeate through the membrane, obtaining grifola frondosa extract ultrafiltration intercepting solution, and drying the intercepting solution to obtain a grifola frondosa extract;

(4) Mixing Ganoderma extract, fructus Momordicae Charantiae extract and Grifola frondosa extract to obtain composition for lowering blood sugar;

wherein the weight percentages of the prepared raw materials are as follows: 20-40% of lucid ganoderma, 40-60% of grifola frondosa and 10-30% of bitter gourd.

8. The method of claim 6 or 7, wherein each of the extracts is prepared as a dry powder or as a granule; preferably, the prepared granules are prepared into granules by adding auxiliary materials and performing wet granulation or one-step granulation.

9. The method of claim 6 or 7, wherein the drying comprises one or more of spray drying, freeze drying, and vacuum drying.

10. Use of the composition of any one of claims 1 to 5 for the preparation of a health food or a pharmaceutical product for lowering or controlling blood glucose or treating diabetes; preferably, the diabetes is the treatment of type II diabetes; preferably, said lowering or controlling blood glucose is lowering or controlling blood glucose in a pre-diabetic population.

Technical Field

The invention relates to a composition, in particular to a composition which has the effect of reducing blood sugar and takes ganoderma lucidum, grifola frondosa and bitter gourd as main raw materials, a preparation method and application thereof.

Background

with the rapid development of economy, the change of life style and the acceleration of aging process, the prevalence rate of diabetes in China is in a rapid rising trend, and the diabetes becomes another chronic non-infectious disease which seriously harms the health of people after cardiovascular and cerebrovascular diseases and tumors. Diabetes is clinically mainly manifested as disorder of carbohydrate metabolism, characterized by hyperglycemia and protein glycosylation, and hyperglycemia is the main marker. The common symptoms include polydipsia, diuresis, polyphagia, emaciation and the like. The increase of blood sugar causes osmotic diuresis, polydipsia and polydipsia, and simultaneously reduces the utilization rate of the blood sugar, thereby stimulating the hunger center to cause diabetes patients to have polyphagia and hunger, and also seriously affecting the synthesis of sugar, fat and protein to cause the decomposition of the sugar, fat and protein to increase and cause emaciation. Chronic complications of diabetes, including diabetic nephropathy, diabetic microangiopathy, diabetic neuropathy, etc., which are also important causes of fatal disability of diabetes, may result from chronic non-treatment or improper treatment. Diabetes and its complications have become a worldwide public health problem seriously harming human health, and have attracted high attention from countries in the world.

Type II diabetes is the most common type of diabetes in clinic, and currently, type II diabetes reaches more than 5 hundred million patients worldwide, while china occupies about 1/3 of the patients. In addition, a large number of pre-diabetic people, namely people who have fasting plasma glucose or (and) have an increased plasma glucose after 2h of an oral glucose tolerance test but do not reach the diagnosis standard of diabetes, exist. According to the report of epidemiological investigation of adults over 18 years old in China, which is completed in 2010, the prevalence rate of diabetes in the early stage in China is 50.1 percent, namely, more than 7 hundred million of people need to perform effective intervention on blood sugar.

At present, the injection of insulin and oral drugs is the main means for treating diabetes, and the effect is also obvious, but the existing drugs, particularly western medicines, are generally accompanied by side effects, and particularly damage is caused to blood vessels, retina and nerve tissues. For the diagnosed diabetes patients, the hypoglycemic medicament should be the first choice, and the hypoglycemic medicament can be assisted by taking safe and health-care food or functional food with no side effect and definite effect. For people in early stage of diabetes mellitus, the Chinese 2-type diabetes mellitus prevention and treatment guideline recommends that patients in early stage of diabetes mellitus should reduce the risk of diabetes mellitus by diet control and exercise, and does not recommend means for preventing diabetes mellitus by using medicaments, so that the blood sugar-reducing health-care food or functional food is the first choice for the people.

Ganoderma is a large-scale fungus of Ganoderma of Ganodermataceae, and has a reputation of Mesona chinensis, and its medicinal part fruiting body has been used for more than two thousand years in China, and has effects of invigorating qi, tranquilizing mind, relieving cough and asthma, giddiness, insomnia, cardiopalmus, short breath, asthenia, cough and asthma, nourishing and strengthening body resistance, and consolidating constitution. Researches show that the indexes of blood sugar, cholesterol, triglyceride, low-density protein and the like of diabetic mice can be reduced by ganoderma lucidum, ganoderma lucidum crude polysaccharide, ganoderma lucidum aqueous extract and the like, and the ganoderma lucidum aqueous extract and the ganoderma lucidum polysaccharide have the function of reducing the blood sugar.

The fruit bodies of the grifola frondosa are soft in meat quality, are crisp like magnolia, like shredded chicken in taste, delicious in taste, unique in fragrance and rich in nutrition, are high-grade rare edible fungi, and meanwhile, the grifola frondosa is a very precious medicinal fungus and has remarkable effects of resisting tumors, reducing blood sugar, resisting hepatitis, resisting HIV viruses, improving immune system functions and the like. Researches show that both the Grifola frondosa fruiting body and Grifola frondosa polysaccharide can obviously reduce the blood sugar level of diabetic mice, and the good blood sugar reducing effect makes the Grifola frondosa fruiting body and the Grifola frondosa polysaccharide have the potential for developing blood sugar reducing medicines.

The balsam pear is sweet and bitter in fruit taste, mature pulp and aril are mainly used as vegetables for eating, and meanwhile, the balsam pear is a traditional Chinese medicine and food dual-purpose plant, is rich in a plurality of active ingredients such as balsam pear polysaccharide, saponin, polypeptide and flavonoid compounds, and has health care functions of assisting in reducing blood sugar, reducing blood fat, resisting oxidation, enhancing immunity, preventing obesity and the like. Research shows that the balsam pear extract can obviously reduce the blood sugar level of diabetic rats, improve the SOD content and the insulin level, and reduce the triglyceride and the cholesterol level. The balsam pear extract has the functions of reducing blood sugar and blood fat of hyperglycemic animals.

At present, the utilization effect of the ganoderma lucidum, the grifola frondosa and the balsam pear is not expected in the prior art, and the report of preparing the hypoglycemic health-care food or medicament by adopting the ganoderma lucidum, the grifola frondosa and the balsam pear is not available.

Disclosure of Invention

Based on the composition, the invention provides a composition with the efficacy of reducing blood sugar, and a preparation method and application thereof.

the invention achieves the above purposes through the following scheme:

In a first aspect, the invention provides a ganoderma lucidum and grifola frondosa hypoglycemic composition, which is prepared from the following raw materials in percentage by weight: 20-40% of lucid ganoderma, 40-60% of grifola frondosa and 10-30% of bitter gourd.

Preferably, the grifola frondosa in the composition is treated by the following method: taking a grifola frondosa raw material, adding 8-20 times of water by weight, heating to 80-105 ℃, extracting for 1-3 times, wherein the single extraction time is 1-4 hours, filtering the extracting solutions, combining, concentrating until the solid content is 5% -20%, obtaining a grifola frondosa extract concentrated solution, performing microfiltration treatment on the grifola frondosa extract concentrated solution, collecting microfiltration permeating solution, performing ultrafiltration on the microfiltration permeating solution, performing interception purification on the extracted components by using an ultrafiltration membrane, wherein the pore size of the ultrafiltration membrane corresponds to the molecular weight intercepted by 1-10KDa, the operating pressure is 3-8MPa, the temperature is 25-40 ℃, retaining ultrafiltration intercepting solution which does not permeate through the membrane, obtaining grifola frondosa extract ultrafiltration intercepting solution, and drying the intercepting solution to obtain the grifola frondosa extract.

Preferably, the concentration is preferably vacuum concentration.

Preferably, the Grifola frondosa raw material is Grifola frondosa fruiting body.

Preferably, the drying comprises one or more of spray drying, freeze drying and vacuum drying.

Preferably, the ganoderma lucidum in the composition is treated by the following method: adding 8-40 times of water into Ganoderma lucidum raw material, extracting at 80-105 deg.C for 1-3 times, each time for 2-6 hr, filtering the extractive solutions, mixing, concentrating to solid content of 5% -25% to obtain Ganoderma lucidum extract concentrate, and drying the Ganoderma lucidum extract concentrate to obtain Ganoderma lucidum extract.

preferably, the bitter gourds in the composition are treated by the following method: taking a bitter gourd raw material, adding 8-20 times of water by weight, extracting for 1-3 times at 80-105 ℃, wherein the extraction time is 1-4 hours each time, filtering the extracting solution, combining, concentrating to the solid content of 5% -25% to obtain a bitter gourd extract concentrated solution, and drying the bitter gourd extract concentrated solution to obtain the bitter gourd extract.

Preferably, the ganoderma lucidum and the momordica charantia in the composition are treated by the following method: mixing Ganoderma and fructus Momordicae Charantiae, adding 8-40 times of water, extracting at 80-105 deg.C for 1-3 times, each for 1-4 hr, filtering the extractive solutions, mixing, concentrating to solid content of 5% -25% to obtain Ganoderma and fructus Momordicae Charantiae extract concentrate, and drying to obtain Ganoderma and fructus Momordicae Charantiae extract.

Preferably, the above extracts can also be made into dry powder or granule.

further preferably, the prepared granules can be prepared into granules by adding auxiliary materials and performing wet granulation or one-step granulation.

Preferably, the ganoderma lucidum raw material is ganoderma lucidum coarse powder.

Preferably, the bitter gourd is a dried bitter gourd slice product.

Preferably, the concentration is a vacuum concentration.

Preferably, the drying comprises one or more of spray drying, freeze drying and vacuum drying.

according to the invention, the lucid ganoderma, the grifola frondosa and the bitter gourd in a specific ratio are compounded for use, so that compared with the single use, the effect of reducing blood sugar is obviously improved, and compared with other edible fungi or fruit and vegetable ratios, the effect of reducing blood sugar is more obvious.

In a second aspect, the invention further provides a preparation method of the ganoderma lucidum and grifola frondosa hypoglycemic composition, which comprises the following steps:

(1) Preparing a grifola frondosa extract: taking a grifola frondosa raw material, adding 8-20 times of water by weight, heating to 80-105 ℃, extracting for 1-3 times, wherein the single extraction time is 1-4 hours, filtering the extracting solutions, combining, concentrating until the solid content is 5% -20%, obtaining a grifola frondosa extract concentrated solution, performing microfiltration treatment on the grifola frondosa extract concentrated solution, collecting microfiltration permeating solution, performing ultrafiltration on the microfiltration permeating solution, performing interception purification on the extracted components by using an ultrafiltration membrane, wherein the pore size of the ultrafiltration membrane corresponds to the molecular weight intercepted by 1-10KDa, the operating pressure is 3-8MPa, the temperature is 25-40 ℃, retaining ultrafiltration intercepting solution which does not permeate through the membrane, obtaining grifola frondosa extract ultrafiltration intercepting solution, and drying the intercepting solution to obtain a grifola frondosa extract;

(2) The ganoderma lucidum and bitter gourd extract: mixing a ganoderma lucidum raw material and a bitter gourd raw material, adding 8-40 times of water by weight, extracting for 1-3 times at 80-105 ℃, wherein the extraction time is 1-4 hours each time, filtering the extracting solution, combining, concentrating until the solid content is 5% -25% to obtain a ganoderma lucidum bitter gourd extract concentrated solution, and drying the ganoderma lucidum bitter gourd extract concentrated solution to obtain a ganoderma lucidum bitter gourd extract;

(3) mixing the extract of Ganoderma and fructus Momordicae Charantiae with the extract of Grifola frondosa to obtain composition;

Wherein the weight percentages of the prepared raw materials are as follows: 20-40% of lucid ganoderma, 40-60% of grifola frondosa and 10-30% of bitter gourd.

preferably, the preparation of the ganoderma lucidum and bitter gourd extract can also be realized by respectively preparing the ganoderma lucidum extract and the bitter gourd extract, and comprises the following steps:

(1) preparing a ganoderma lucidum extract: adding 8-40 times of water into Ganoderma lucidum raw material, extracting at 80-105 deg.C for 1-3 times, each time for 2-6 hr, filtering the extractive solutions, mixing, concentrating to solid content of 5% -25% to obtain Ganoderma lucidum extract concentrate, and drying the Ganoderma lucidum extract concentrate to obtain Ganoderma lucidum extract;

(2) Preparing a bitter gourd extract: taking a bitter gourd raw material, adding 8-20 times of water by weight, extracting for 1-3 times at 80-105 ℃, wherein the extraction time is 1-4 hours each time, filtering the extracting solution, combining, concentrating to the solid content of 5% -25% to obtain a bitter gourd extract concentrated solution, and drying the bitter gourd extract concentrated solution to obtain the bitter gourd extract.

Under the condition that the ganoderma lucidum extract and the bitter gourd extract are respectively prepared, the preparation method of the ganoderma lucidum and grifola frondosa hypoglycemic composition comprises the following steps:

(1) Preparing a ganoderma lucidum extract: adding 8-40 times of water into Ganoderma lucidum raw material, extracting at 80-105 deg.C for 1-3 times, each time for 2-6 hr, filtering the extractive solutions, mixing, concentrating to solid content of 5% -25% to obtain Ganoderma lucidum extract concentrate, and drying the Ganoderma lucidum extract concentrate to obtain Ganoderma lucidum extract;

(2) Preparing a bitter gourd extract: taking a bitter gourd raw material, adding 8-20 times of water by weight, extracting for 1-3 times at 80-105 ℃, wherein the extraction time is 1-4 hours each time, filtering the extracting solution, combining, concentrating to the solid content of 5% -25% to obtain a bitter gourd extract concentrated solution, and drying the bitter gourd extract concentrated solution to obtain a bitter gourd extract;

(3) Preparing a grifola frondosa extract: taking a grifola frondosa raw material, adding 8-20 times of water by weight, heating to 80-105 ℃, extracting for 1-3 times, wherein the single extraction time is 1-4 hours, filtering the extracting solutions, combining, concentrating until the solid content is 5% -20%, obtaining a grifola frondosa extract concentrated solution, performing microfiltration treatment on the grifola frondosa extract concentrated solution, collecting microfiltration permeating solution, performing ultrafiltration on the microfiltration permeating solution, performing interception purification on the extracted components by using an ultrafiltration membrane, wherein the pore size of the ultrafiltration membrane corresponds to the molecular weight intercepted by 1-10KDa, the operating pressure is 3-8MPa, the temperature is 25-40 ℃, retaining ultrafiltration intercepting solution which does not permeate through the membrane, obtaining grifola frondosa extract ultrafiltration intercepting solution, and drying the intercepting solution to obtain a grifola frondosa extract;

(4) Mixing Ganoderma extract, fructus Momordicae Charantiae extract and Grifola frondosa extract to obtain composition for lowering blood sugar;

Wherein the weight percentages of the prepared raw materials are as follows: 20-40% of lucid ganoderma, 40-60% of grifola frondosa and 10-30% of bitter gourd

Preferably, the above extracts can also be made into dry powder or granule.

Further preferably, the prepared granules can be prepared into granules by adding auxiliary materials and performing wet granulation or one-step granulation.

Preferably, the concentration is preferably vacuum concentration.

Preferably, the drying comprises one or more of spray drying, freeze drying and vacuum drying.

preferably, the Grifola frondosa raw material is Grifola frondosa fruiting body.

Preferably, the ganoderma lucidum raw material is ganoderma lucidum coarse powder.

Preferably, the bitter gourd is a dried bitter gourd slice product.

In a third aspect, a hypoglycemic composition prepared by the preparation method is provided.

In a fourth aspect, there is provided the use of the above composition for lowering or controlling blood glucose, or treating diabetes.

Preferably, the diabetes is the treatment of type II diabetes.

Preferably, said reducing or controlling blood glucose is reducing or controlling blood glucose in a pre-diabetic population

the application of the composition is used for preparing health-care food or medicine for reducing or controlling blood sugar or treating diabetes.

Preferably, the diabetes is the treatment of type II diabetes.

Preferably, said lowering or controlling blood glucose is lowering or controlling blood glucose in a pre-diabetic population.

In a preferred embodiment, the use of the above composition for the preparation of a health food for reducing or controlling blood glucose in pre-diabetic persons.

in a preferred embodiment, the composition is used for preparing health-care food or medicine for treating type II diabetes.

on the basis of the composition, food or pharmaceutically acceptable auxiliary materials are added through the addition or preparation method of the conventional auxiliary materials of various formulations to obtain health-care food or medicaments, including but not limited to tablets, powder, granules and capsules.

compared with the prior art, the invention has the beneficial effects that:

(1) the invention utilizes edible and medicinal fungi grifola frondosa, lucid ganoderma and food balsam pear with efficacy as raw materials, has the characteristics of naturalness and safety, and simultaneously, the edible fungi and natural hypoglycemic substances are compounded, so that the efficacy of all the raw materials in the aspect of reducing blood sugar is fully exerted, and the invention has good coordination effect of reducing blood sugar.

(2) The microfiltration and ultrafiltration interception technologies adopted in the preparation method have the characteristics of simple operation, low energy consumption, short production period and the like, and can separate and protect active ingredients in the grifola frondosa extract to the maximum extent and remove ingredients with poor efficacy.

(3) Animal experiment research shows that the composition has obvious blood sugar reducing effect, can obviously improve II diabetes rat model glycolipid metabolism pathological state induced by alloxan intraperitoneal injection and high-fat feed feeding, and has obvious blood sugar reducing effect.

Detailed Description

The present invention is further illustrated by the following specific examples.