阅读说明：本技术 一种含***提取物的消炎喷雾剂及其制备方法 (Anti-inflammatory spray containing cannabis extract and preparation method thereof ) 是由 王钲霖 刘胜贵 付彬彬 李智高 孔令羽 于 2019-10-12 设计创作，主要内容包括：本发明公开了一种含大麻提取物的消炎喷雾剂及其制备方法,属于医药领域。它由如下重量份的原辅料组成：乙醇60～90份；大麻提取物2～15份；冬青油1～5份；冰片0.5～3份；樟脑0.5～3份；氮酮0.3～0.8份；香精0.1～0.5份。该喷雾剂针对皮肤伤口有极强的止痛及消炎功效,能预防伤口感染、红肿,快速实现伤口结痂愈合。同时,该喷雾对蚊虫叮咬,擦伤有一定的治疗效果。本发明工艺简单,原料易得,成本低廉,适合工业化生产。(the invention discloses an anti-inflammatory spray containing a cannabis extract and a preparation method thereof, belonging to the field of medicines. The composition comprises the following raw and auxiliary materials in parts by weight: 60-90 parts of ethanol; 2-15 parts of a hemp extract; 1-5 parts of wintergreen oil; 0.5-3 parts of borneol; 0.5-3 parts of camphor; 0.3-0.8 part of azone; 0.1-0.5 parts of essence. The spray has strong analgesic and anti-inflammatory effects on skin wound, and can prevent wound infection and red swelling, and rapidly realize wound scabbing and healing. Meanwhile, the spray has a certain treatment effect on mosquito bites and scratches. The method has the advantages of simple process, easily obtained raw materials and low cost, and is suitable for industrial production.)

1. An anti-inflammatory spray containing cannabis extract and a preparation method thereof, which comprises the following raw and auxiliary materials in parts by weight:

60-90 parts of ethanol; 2-15 parts of a hemp extract; 1-5 parts of wintergreen oil; 0.5-3 parts of borneol; 0.5-3 parts of camphor; 0.3-0.8 part of azone; 0.1-0.5 parts of essence.

2. The anti-inflammatory spray containing cannabis extract and the preparation method thereof as claimed in claim 1, wherein the preparation method comprises the following steps:

(1) Preparation of cannabis extracts

Fresh industrial hemp flower and leafDryingPulverizingDrying by bakingCO₂Supercritical extractionDewaxingDecolorization of

(2) Adding the obtained cannabis extract into ethanol with the temperature of 50 ℃ according to a proportion, and continuously stirring until the cannabis extract is dissolved into a transparent solution;

(3) Sequentially adding borneol, camphor and azone according to the proportion, and stirring until the borneol, the camphor and the azone are dissolved;

(4) Cooling to 30 deg.C, sequentially adding wintergreen oil and essence, stirring, and cooling to room temperature.

3. The anti-inflammatory spray containing cannabis extract and the preparation method thereof as claimed in claim 2, wherein in the step (1), the content of Tetrahydrocannabinol (THC) in fresh industrial cannabis flowers and leaves refers to the content of Tetrahydrocannabinol (THC) in flowers and leaves is less than 0.3%.

4. The anti-inflammatory spray containing cannabis sativa extract and the preparation method thereof as claimed in claim 2, wherein in the step (1), the drying is performed at 100-120 ℃ for 0.5-4 h for fresh industrial cannabis sativa flowers and leaves, so that the water content is 6-10%.

5. The anti-inflammatory spray containing cannabis extract and the preparation method thereof as claimed in claim 2, wherein in the step (1), the pulverization is to pulverize the dried leaves to 50-100 mesh.

6. The anti-inflammatory spray containing cannabis sativa extract and the preparation method thereof as claimed in claim 2, wherein the drying in step (1) is to dry the pulverized leaves at 100-120 ℃ for 0.5-2 h again to make the water content less than 5%.

7. The anti-inflammatory spray containing cannabis extract and the preparation method thereof as claimed in claim 2, wherein in the step (1), CO is used₂supercritical extraction refers to the utilization of CO in supercritical state₂Extracting industrial hemp leaves and leaves by using a mobile phase to obtain an extract; the pressure of the extraction kettle is 15-30 MPa, the extraction temperature is 35-60 ℃, and the extraction time is 0.5-3 hours; the temperature of the desorption kettle is 35-50 ℃, and the desorption pressure is 1-15 MPa.

8. The anti-inflammatory spray containing cannabis extract and the preparation method thereof as claimed in claim 2, wherein in the step (1), dewaxing is performed by winterization treatment, namely, supercritical extract is dissolved by 2-7 times of ethanol, the temperature is kept at-20 ℃ for 2h, and the anti-inflammatory spray is filtered and filtrate is collected to obtain the anti-inflammatory spray.

9. the anti-inflammatory spray containing cannabis extract and the preparation method thereof as claimed in claim 2, wherein in the step (1), activated carbon adsorption is adopted for decolorization; adding 3-8% of activated carbon by mass into the dewaxing filtrate, heating to 45 ℃, stirring for 30min, filtering while hot, collecting the filtrate, and concentrating to obtain the dewaxing filtrate.

Technical Field

the invention belongs to the field of medicines, and particularly relates to an anti-inflammatory spray containing a cannabis extract and a preparation method thereof.

Background

In life, people cause epidermis injuries due to collision, scratch or mosquito bites, once skin is injured, the skin is easily infected by bacteria or fungi, wounds are difficult to heal, the wounds are more painful, and other infectious diseases can be caused in serious cases. And skin diseases such as dermatophytosis dermatitis and the like caused by bad living habits, such as unworkable self-cleaning, improper clothes cleaning and the like, or skin diseases caused by careless contact with articles used by people suffering from infectious skin diseases, and the skin diseases bring great inconvenience to life and work.

The plant source antibacterial substance widely exists in the nature and is a lead source for creating the green bactericide. The antibacterial compounds in plants are mostly secondary metabolites of plants, and comprise alkaloids, flavonoids, proteins, organic acids, phenols and other compounds. The secondary metabolites of plants are over 400000. Finding bacteriostatic and bactericidal active substances from plants is one of the important ways for researching and developing green preservatives and bacteriostatic agents at present. China has abundant Chinese herbal plant resources, and most of plants used for researches on antibacterial and bacteriostatic activities are concentrated in more than 10 families of plants such as Compositae, Labiatae and Magnoliaceae. Many medicinal plants have antibacterial and bactericidal effects. However, at present, researches on bacteriostasis of Chinese herbal medicines are still not much.

The cannabis extract is a cannabinoids extracted from natural green plant industrial cannabis leaves. The main active component of the compound Cannabidiol (CBD) has super-strong antibacterial ability, has broad-spectrum bactericidal effect on various bacteria and viruses, can inhibit the growth of the bacteria, and can diminish inflammation and relieve pain on skin inflammation caused by the bacteria.

Currently, existing anti-inflammatory sprays suffer from certain deficiencies, such as; the existing anti-inflammatory spray has the advantages of fast anti-inflammatory speed, good analgesic effect, convenient use, high use safety and the like; most of the existing anti-inflammatory sprays adopt synthetic drugs such as antibiotics, and the problem of drug resistance enhancement of bacteria and viruses and the like can be caused by long-term use.

Disclosure of Invention

In order to solve the problems, the invention provides an anti-inflammatory spray containing a cannabis extract and a preparation method thereof. The spray has strong analgesic and anti-inflammatory effects on skin wound, and can prevent wound infection and red swelling, and rapidly realize wound scabbing and healing. Meanwhile, the spray has certain treatment effect on mosquito bites and scratches. The method has the advantages of simple process, easily obtained raw materials and low cost, and is suitable for industrial production.

an anti-inflammatory spray containing a cannabis extract is mainly realized by the following technical scheme, and comprises the following raw materials in parts by weight:

60-90 parts of ethanol; 2-15 parts of a hemp extract; 1-5 parts of wintergreen oil; 0.5-3 parts of borneol; 0.5-3 parts of camphor; 0.3-0.8 part of azone; 0.1-0.5 parts of essence.

The hemp extract is prepared by drying and pulverizing industrial hemp flower and leaf, and using supercritical CO₂The extract obtained by extraction method or solvent extraction method is obtained by dewaxing and decolorizing.

The main component methyl salicylate of wintergreen oil has the functions of dilating skin blood vessels, promoting local blood circulation, and reflectively affecting skin, muscle, nerve and joints at corresponding parts, thereby having the functions of diminishing inflammation, easing pain and relieving itching.

The Borneolum has refreshing feeling, and has antibacterial, antiseptic, antiinflammatory, and heat clearing effects.

The camphor is granular crystal prepared from branches, stems, leaves and roots of Cinnamomum camphora of Lauraceae by extracting. The Camphora has effects of dredging orifices, promoting qi stagnation, removing filth, killing parasites, relieving itching, and relieving swelling and pain.

An anti-inflammatory spray containing cannabis extract and a preparation method thereof, comprising the following preparation steps:

(1) Preparation of cannabis extracts

Fresh industrial hemp flower and leafdryingPulverizingDrying by bakingCO₂Supercritical extractionDewaxingDecolorization of

(2) Adding the obtained cannabis extract into ethanol with the temperature of 50 ℃ according to the proportion, and continuously stirring until the cannabis extract is dissolved into transparent solution.

(3) Adding Borneolum Syntheticum, Camphora, and azone in sequence at a certain proportion, stirring to dissolve.

(4) Cooling to 30 deg.C, sequentially adding wintergreen oil and essence, stirring, and cooling to room temperature.

In the step (1), the content of the Tetrahydrocannabinol (THC) in the fresh industrial hemp flowers and leaves refers to that the content of the THC in the flowers and leaves is less than 0.3%.

In the step (1), the drying is carried out at the temperature of 100-120 ℃ for 0.5-4 h, so that the water content is 6-10%.

In the step (1), the flower leaves dried in the previous step are crushed to 50-100 meshes.

In the step (1), the drying means drying the crushed flower leaves for 0.5-2 hours at 100-120 ℃ so that the water content is less than 5%.

In the step (1), CO₂Supercritical extraction refers to the utilization of CO in supercritical state₂extracting industrial hemp flower and leaf with mobile phase to obtain extract. The pressure of the extraction kettle is 15-30 MPa, the extraction temperature is 35-60 ℃, and the extraction time is 0.5-3 hours. The temperature of the desorption kettle is 35-50 ℃, and the desorption pressure is 1-15 MPa.

In the step (1), dewaxing is carried out by a winterization treatment method, namely dissolving the supercritical extract by using 2-7 times of ethanol, preserving heat at the temperature of-20 ℃ for 2 hours, filtering, and collecting filtrate.

In the step (1), activated carbon adsorption is adopted for decolorization. Adding 3-8% of activated carbon by mass into the dewaxing filtrate, heating to 45 ℃, stirring for 30min, filtering while hot, collecting the filtrate, and concentrating to obtain the dewaxing filtrate.

The method has the advantages and the technical effects that:

(1) The spray prepared by the invention has extremely strong pain relieving and inflammation diminishing effects on skin wounds, can prevent wound infection and red swelling, and can quickly realize wound scabbing and healing.

(2) The main active ingredient hemp extract of the spray prepared by the invention adopts supercritical CO₂The extract has no solvent residue, and no irritation to skin.

(3) The spray prepared by the invention adopts the extract of the pure natural green plant cannabis sativa, is natural and green, has no toxic or side effect, is easy to obtain raw materials, has low cost and is suitable for market popularization.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments.