阅读说明：本技术 一种病毒检测传感器、一种检测病毒浓度的装置和方法 (virus detection sensor, device and method for detecting virus concentration ) 是由 洪少力 秦莉 张泽芬 张琴韵 汤曼 张南刚 刘侃 于 2019-08-27 设计创作，主要内容包括：本发明公开了一种病毒检测传感器、一种检测病毒浓度的装置和方法,所述传感器包括支架、检测探头和毛细玻璃管,毛细玻璃管内壁上修饰有与待测病毒进行特异性结合的抗体,检测探头包括检测体和光电转化电路,光电转化电路包括光敏元件和光源,检测体固定于支架上,检测体中央设有圆形的检测通道,毛细玻璃管穿过检测通道,毛细玻璃管固定于支架上,检测通道侧壁上对称第一凹槽和第二凹槽,光源固定于第一凹槽中,光敏元件固定于第二凹槽中。该传感器结构简单,设计巧妙,将磁球的遮光性与光电转换巧妙地结合,检测方便快捷。该装置结构简单,能实现病毒地自动化检测,提高了检测的效率和精度。该方法简单,利用标准曲线法进行检测,方便快捷。(the invention discloses a virus detection sensor, a device and a method for detecting virus concentration, wherein the sensor comprises a bracket, a detection probe and a capillary glass tube, an antibody which is specifically combined with a virus to be detected is modified on the inner wall of the capillary glass tube, the detection probe comprises a detection body and a photoelectric conversion circuit, the photoelectric conversion circuit comprises a photosensitive element and a light source, the detection body is fixed on the bracket, a circular detection channel is arranged in the center of the detection body, the capillary glass tube passes through the detection channel, the capillary glass tube is fixed on the bracket, a first groove and a second groove are symmetrically arranged on the side wall of the detection channel, the light source is fixed in the first groove, and the photosensitive element is fixed in the second groove. The sensor has the advantages of simple structure, ingenious design, skillful combination of the light shielding property of the magnetic ball and the photoelectric conversion, and convenient and quick detection. The device simple structure can realize the automated inspection of virus ground, has improved the efficiency and the precision of detection. The method is simple, and convenient and rapid to detect by using a standard curve method.)

1. a virus detection sensor, characterized by: the detection probe comprises a detection body and a photoelectric conversion circuit, the photoelectric conversion circuit comprises a photosensitive element and a light source, the detection body is fixed on the support, a circular detection channel is arranged in the center of the detection body, the capillary glass tube penetrates through the detection channel, the capillary glass tube is in clearance fit with the detection channel, the capillary glass tube is fixed on the support, a first groove and a second groove are symmetrically formed in the side wall of the detection channel, the light source is fixed in the first groove, the photosensitive element is fixed in the second groove, and the light source is opposite to the photosensitive element.

2. The virus detection sensor according to claim 1, wherein: the support include backup pad and anchor clamps, anchor clamps have two, anchor clamps include the supporter and have elastic C type clamp, be equipped with semicircular third recess on the supporter, the diameter of third recess equals with the external diameter of capillary glass pipe, the supporter symmetry is fixed in the backup pad, the detection body is located between two supporter, two third recesses are arranged respectively in to the both sides of capillary glass pipe, and the both sides of capillary glass pipe are fixed in on two supporter through C type clamp respectively.

3. an apparatus for detecting a concentration of a virus, comprising: comprises the virus detection sensor, a power mechanism, a valve control mechanism, a reagent supply mechanism, a magnetic separation mechanism and a detection mechanism of claim 1;

The power mechanism comprises an air extracting pump, a first air extracting branch pipe and a first valve, one end of the first air extracting branch pipe is communicated with an outlet of the air extracting pump, and the first valve is arranged on the first air extracting branch pipe;

the reagent supply mechanism comprises N reagent bottles, and the other ends of the first air exhaust branch pipes are respectively communicated with the gas paths of the N reagent bottles;

The magnetic separation mechanism comprises a micro-fluidic chip, a sample discharge pipe and a waste discharge pipe, each reagent bottle is controlled by a valve control mechanism to be communicated with a liquid path at the inlet of the micro-fluidic chip, the micro-fluidic chip comprises a buffer liquid moving channel and a sample moving channel, the buffer liquid moving channel is communicated with the inlet of the capillary glass pipe through the sample discharge pipe, and the sample moving channel is communicated with the waste discharge pipe.

4. The apparatus for detecting a concentration of a virus according to claim 1, wherein: the valve control mechanism comprises a multi-path pneumatic electromagnetic valve, a controller and a valve control chip, wherein the controller is connected with the multi-path pneumatic electromagnetic valve, and the multi-path pneumatic electromagnetic valve is provided with an air inlet and N air outlets;

The valve control chip comprises a clamp and a glass chip, wherein the glass chip comprises a cover plate, an organic silicon film and a substrate, the glass chip is of a sandwich structure, the organic silicon film is positioned between the cover plate and the substrate, and the cover plate and the substrate are fixedly connected through the clamp;

The cover plate is respectively provided with N first liquid inlet holes, two first liquid outlet holes and N gas through grooves, the N gas through grooves are sealed through a clamp, and the organic silicon film is respectively provided with N second liquid inlet holes and two second liquid outlet holes;

The substrate is respectively provided with a first liquid channel and a second liquid channel, the first liquid channel is composed of a first main channel, a first liquid outlet channel and Q first liquid inlet branch channels, one end of each first liquid inlet branch channel is respectively communicated with the first main channel, one first liquid inlet branch channel is communicated with one end of the first main channel, one end of the first liquid outlet channel is communicated with the other end of the first main channel, the second liquid channel is composed of a second main channel, a second liquid outlet channel and P second liquid inlet branch channels, one end of each second liquid inlet branch channel is respectively communicated with the second main channel, one second liquid inlet branch channel is communicated with one end of the second main channel, one end of the second liquid outlet channel is communicated with the other end of the second main channel, and Q + P is N;

the N reagent bottles are respectively communicated with the N first liquid inlet holes, the N first liquid inlet holes are respectively communicated with the N second liquid inlet holes, Q second liquid inlet holes are respectively communicated with the other ends of the Q first liquid inlet sub-channels, and the remaining P second liquid inlet holes are respectively communicated with the other ends of the P second liquid inlet sub-channels;

the N gas outlets are respectively communicated with the N gas through grooves, and the projection of each gas through groove on the substrate separates the corresponding first liquid inlet sub-channel or second liquid inlet sub-channel;

The power mechanism also comprises a second air exhaust branch pipe and a second valve, one end of the second air exhaust branch pipe is communicated with the outlet of the air exhaust pump, the second valve is arranged on the second air exhaust branch pipe, and the other end of the second air exhaust branch pipe is communicated with the air inlet;

The micro-fluidic chip further comprises a first injection port, a second injection port, a first discharge port and a second discharge port, wherein the first injection port and the first discharge port are respectively communicated with two ends of the buffer liquid moving channel, the second injection port and the second discharge port are respectively communicated with two ends of the sample moving channel, the other ends of the first liquid outlet channel and the second liquid outlet channel are respectively communicated with two second liquid discharge holes, the two second liquid discharge holes are respectively communicated with the two first liquid discharge holes, one of the first liquid discharge holes is communicated with the first injection port, the first discharge port is communicated with one end of the sample discharge pipe, the other end of the sample discharge pipe is communicated with the capillary glass pipe, the other first liquid discharge hole is communicated with the second injection port, and the second discharge port is communicated with the waste discharge pipe.

5. The apparatus for detecting a concentration of a virus according to claim 4, wherein: anchor clamps include first splint and second splint, the glass chip is located between first splint and the second splint, first splint are laminated with the cover plate is sealed, the second splint are laminated with the substrate is sealed, first splint and second splint pass through bolt fixed connection, be connected with N feed liquor pipe on the first splint, two fluid-discharge tubes and N intake pipes, N reagent bottle is connected with the one end of N feed liquor pipe respectively, the other end of N feed liquor pipe is N first feed liquor jogged joints respectively, the one end of two fluid-discharge tubes communicates with two first fluid-discharge holes respectively, the other end of two fluid-discharge tubes communicates with first filling opening and second filling opening respectively, the one end of N intake pipe communicates with N gas outlet respectively, the other end of N intake pipe communicates with N gaseous logical groove respectively.

6. the apparatus for detecting a concentration of a virus according to claim 4, wherein: the reagent supply mechanism further comprises N liquid injection pipes and N-1 communicating pipes, one ends of the N liquid injection pipes are communicated with the N reagent bottles respectively, the other ends of the N liquid injection pipes are communicated with one ends of the N liquid inlet pipes respectively, the other end of the first air exhaust branch pipe is communicated with the bottle opening of the first reagent bottle, and the bottle openings of the two adjacent reagent bottles are communicated through the communicating pipes.

7. the apparatus for detecting a concentration of a virus according to claim 4, wherein: still include the diwen screen, virus detection sensor is connected with the controller, and the controller is connected with the diwen screen.

8. a method for detecting the concentration of a virus, comprising the steps of:

8.1, modifying the magnetic microspheres in the magnetic microsphere suspension with an antibody capable of specifically binding with the virus to be detected to obtain an antibody modified magnetic microsphere suspension;

8.2, adding the antibody modified magnetic ball suspension into the virus stock solution containing the virus to be detected for reaction, enabling the virus to be detected to be completely specifically combined with the antibody, and simultaneously diluting the virus stock solution to the virus concentration C₁、C₂……C_mvirus standard solution of (3), C₁、C₂……C_msuccessively increase, C₁≤5ng/ml，C_m≥155ng/ml；

8.3 adding blocking reagent to one pair of reagent bottles, adding deionized water and PBS buffer solution to one pair of reagent bottlesadding a volume V and a concentration C into a reagent bottle₁The virus standard solution of (2) is to contain C₁The reagent bottle of the virus standard solution is marked as a sample bottle;

8.4, controlling the valve control mechanism to enable the two reagent bottles containing the closed reagent to be communicated with the inlet of the microfluidic chip, enabling the two reagent bottles containing the closed reagent to be respectively communicated with the buffer solution moving channel and the sample moving channel, starting the air pump, respectively introducing the closed reagent into the communication between the buffer solution moving channel and the sample moving channel, reducing non-specific adsorption, and after the closing is finished, controlling the valve control mechanism to enable the two reagent bottles containing the closed reagent to be separated from the inlet of the microfluidic chip;

8.5, controlling a valve control mechanism to enable two reagent bottles containing deionized water and PBS buffer solution to be communicated with the inlet of the microfluidic chip, enabling the reagent bottle containing the PBS buffer solution to be communicated with a buffer solution moving channel, enabling the reagent bottle containing the deionized water to be communicated with a sample moving channel, introducing the PBS buffer solution into the buffer solution moving channel, introducing an ionic water sample into the moving channel, communicating a cleaning buffer solution moving channel with the sample moving channel, and after cleaning, controlling the valve control mechanism to enable the two reagent bottles containing the deionized water and the PBS buffer solution to be separated from the inlet of the microfluidic chip;

8.6, controlling a valve control mechanism to enable two reagent bottles containing the virus standard solution and the PBS buffer solution to be communicated with the inlet of the microfluidic chip, enabling the reagent bottles containing the virus standard solution to be communicated with the sample moving channel, enabling the reagent bottles containing the PBS buffer solution to be communicated with the buffer moving channel, introducing the PBS buffer solution into the buffer moving channel, introducing virus standard solution into the sample moving channel, enriching the magnetic microspheres in the virus standard solution in the buffer solution moving channel and flowing into the capillary glass tube, capturing the magnetic microspheres at the capillary glass tube, when the virus standard solution is completely introduced, the control valve control mechanism is controlled to separate the reagent bottle containing the virus standard solution from the inlet of the microfluidic chip, when the voltage at the two ends of the photosensitive element is not changed any more, the control valve control mechanism cuts off the reagent bottle containing the PBS buffer solution from the inlet of the microfluidic chip and records the voltage V at the two ends of the photosensitive element.₁；

8.7, after cleaning the sample bottle, adding V volume and C concentration into the sample bottle₂the virus standard solution of (4); or directly adding V volume and C concentration into one of the reagent bottles₂The virus standard solution of (4);

8.8, repeating step 8.4-8.6, wherein the voltage across the photosensitive element is measured to be V₂；

8.9, repeating the steps 8.7-8.8 until all the virus standard solutions are detected, and respectively measuring the voltage at two ends of the photosensitive element as V₃……V_m；

8.10, drawing according to the detected data by taking the concentration of the virus standard solution as an abscissa and taking the resistance at two ends of the photosensitive element as an ordinate, fitting to obtain a standard curve, and obtaining a functional relation y ═ k of the standard curve according to the standard curve₁x+b₁；

8.11, detecting the virus liquid to be detected by the method of the steps 8.3-8.6, measuring the voltage at two ends of the photosensitive element as V ', and substituting the V' into the functional relation to obtain the concentration of the virus in the virus liquid to be detected.

Technical Field

the invention relates to the technical field of biological detection, in particular to a virus detection sensor, a device and a method for detecting virus concentration.

background

At present, human diseases caused by viruses are various, different viruses have different pathogenic mechanisms, and at a cellular level, the main destructive effect of the viruses is to cause cell lysis and cell death. In multicellular organisms, once sufficient cells in the body have died, the health of the body is affected. Some viruses are capable of causing chronic infections and can replicate constantly in the body without being affected by the host defense system. People with chronic infections are virus carriers, since they are equivalent to storing virus that remains infectious, and when there is a high proportion of carriers in the population, the disease can develop into an epidemic. Therefore, the virus detection technology has important significance in the biomedical field and is an important ring for ensuring human health.

most of the current clinical detection methods are nucleic acid detection and protein detection, and the detection process has high technical and equipment requirements. Currently common viral technologies include: a virus detection method and device (patent CN201510144530), a virus detection device and use method thereof (CN201711486707), a virus character detection device (CN201820145057) and the like. However, the above device is complicated in implementation process, high in technical requirement, low in efficiency, high in cost, increased in inspection cost and not beneficial to popularization.

Disclosure of Invention

In order to solve the problems in the prior art, the invention provides a virus detection sensor, a device and a method for detecting virus concentration.

the device simple structure can realize the automated inspection of virus ground, has improved the efficiency and the precision of detection of detecting.

The method is simple, and convenient and rapid to detect by using a standard curve method.

the technical scheme adopted for realizing the above purpose of the invention is as follows:

The utility model provides a virus detection sensor, which comprises a bracket, test probe and capillary glass tube, the decoration has the antibody that carries out the specificity with the virus that awaits measuring to combine on the capillary glass tube inner wall, test probe includes detection body and photoelectric conversion circuit, photoelectric conversion circuit includes light sensing element and light source, the detection body is fixed in on the support, detection body central authorities are equipped with circular shape detection channel, capillary glass tube passes detection channel, capillary glass tube and detection channel clearance fit, capillary glass tube is fixed in on the support, first recess of symmetry and second recess on the detection channel lateral wall, the light source is fixed in first recess, in light sensing element is fixed in the second recess, the light source is just to light sensing element.

The support include backup pad and anchor clamps, anchor clamps have two, anchor clamps include the supporter and have elastic C type clamp, be equipped with semicircular third recess on the supporter, the diameter of third recess equals with the external diameter of capillary glass pipe, the supporter symmetry is fixed in the backup pad, the detection body is located between two supporter, two third recesses are arranged respectively in to the both sides of capillary glass pipe, and the both sides of capillary glass pipe are fixed in on two supporter through C type clamp respectively.

Comprises the virus detection sensor, a power mechanism, a valve control mechanism, a reagent supply mechanism, a magnetic separation mechanism and a detection mechanism of claim 1;

The power mechanism comprises an air extracting pump, a first air extracting branch pipe and a first valve, one end of the first air extracting branch pipe is communicated with an outlet of the air extracting pump, and the first valve is arranged on the first air extracting branch pipe;

The reagent supply mechanism comprises N reagent bottles, and the other ends of the first air exhaust branch pipes are respectively communicated with the gas paths of the N reagent bottles;

the magnetic separation mechanism comprises a micro-fluidic chip, a sample discharge pipe and a waste discharge pipe, each reagent bottle is controlled by a valve control mechanism to be communicated with a liquid path at the inlet of the micro-fluidic chip, the micro-fluidic chip comprises a buffer liquid moving channel and a sample moving channel, the buffer liquid moving channel is communicated with the inlet of the capillary glass pipe through the sample discharge pipe, and the sample moving channel is communicated with the waste discharge pipe.

The valve control mechanism comprises a multi-path pneumatic electromagnetic valve, a controller and a valve control chip, wherein the controller is connected with the multi-path pneumatic electromagnetic valve, and the multi-path pneumatic electromagnetic valve is provided with an air inlet and N air outlets;

the valve control chip comprises a clamp and a glass chip, wherein the glass chip comprises a cover plate, an organic silicon film and a substrate, the glass chip is of a sandwich structure, the organic silicon film is positioned between the cover plate and the substrate, and the cover plate and the substrate are fixedly connected through the clamp;

the cover plate is respectively provided with N first liquid inlet holes, two first liquid outlet holes and N gas through grooves, the N gas through grooves are sealed through a clamp, and the organic silicon film is respectively provided with N second liquid inlet holes and two second liquid outlet holes;

The substrate is respectively provided with a first liquid channel and a second liquid channel, the first liquid channel is composed of a first main channel, a first liquid outlet channel and Q first liquid inlet branch channels, one end of each first liquid inlet branch channel is respectively communicated with the first main channel, one first liquid inlet branch channel is communicated with one end of the first main channel, one end of the first liquid outlet channel is communicated with the other end of the first main channel, the second liquid channel is composed of a second main channel, a second liquid outlet channel and P second liquid inlet branch channels, one end of each second liquid inlet branch channel is respectively communicated with the second main channel, one second liquid inlet branch channel is communicated with one end of the second main channel, one end of the second liquid outlet channel is communicated with the other end of the second main channel, and Q + P is N;

the N reagent bottles are respectively communicated with the N first liquid inlet holes, the N first liquid inlet holes are respectively communicated with the N second liquid inlet holes, Q second liquid inlet holes are respectively communicated with the other ends of the Q first liquid inlet sub-channels, and the remaining P second liquid inlet holes are respectively communicated with the other ends of the P second liquid inlet sub-channels;

the N gas outlets are respectively communicated with the N gas through grooves, and the projection of each gas through groove on the substrate separates the corresponding first liquid inlet sub-channel or second liquid inlet sub-channel;

The power mechanism also comprises a second air exhaust branch pipe and a second valve, one end of the second air exhaust branch pipe is communicated with the outlet of the air exhaust pump, the second valve is arranged on the second air exhaust branch pipe, and the other end of the second air exhaust branch pipe is communicated with the air inlet;

the micro-fluidic chip further comprises a first injection port, a second injection port, a first discharge port and a second discharge port, wherein the first injection port and the first discharge port are respectively communicated with two ends of the buffer liquid moving channel, the second injection port and the second discharge port are respectively communicated with two ends of the sample moving channel, the other ends of the first liquid outlet channel and the second liquid outlet channel are respectively communicated with two second liquid discharge holes, the two second liquid discharge holes are respectively communicated with the two first liquid discharge holes, one of the first liquid discharge holes is communicated with the first injection port, the first discharge port is communicated with one end of the sample discharge pipe, the other end of the sample discharge pipe is communicated with the capillary glass pipe, the other first liquid discharge hole is communicated with the second injection port, and the second discharge port is communicated with the waste discharge pipe.

Anchor clamps include first splint and second splint, the glass chip is located between first splint and the second splint, first splint are laminated with the cover plate is sealed, the second splint are laminated with the substrate is sealed, first splint and second splint pass through bolt fixed connection, be connected with N feed liquor pipe on the first splint, two fluid-discharge tubes and N intake pipes, N reagent bottle is connected with the one end of N feed liquor pipe respectively, the other end of N feed liquor pipe is N first feed liquor jogged joints respectively, the one end of two fluid-discharge tubes communicates with two first fluid-discharge holes respectively, the other end of two fluid-discharge tubes communicates with first filling opening and second filling opening respectively, the one end of N intake pipe communicates with N gas outlet respectively, the other end of N intake pipe communicates with N gaseous logical groove respectively.

The reagent supply mechanism further comprises N liquid injection pipes and N-1 communicating pipes, one ends of the N liquid injection pipes are communicated with the N reagent bottles respectively, the other ends of the N liquid injection pipes are communicated with one ends of the N liquid inlet pipes respectively, the other end of the first air exhaust branch pipe is communicated with the bottle opening of the first reagent bottle, and the bottle openings of the two adjacent reagent bottles are communicated through the communicating pipes.

Still include the diwen screen, virus detection sensor is connected with the controller, and the controller is connected with the diwen screen.

A method for detecting the concentration of a virus comprising the steps of:

1. modifying the magnetic microspheres in the magnetic microsphere suspension with an antibody capable of specifically binding with the virus to be detected to obtain an antibody modified magnetic microsphere suspension;

2. adding the antibody modified magnetic ball suspension into the virus stock solution containing the virus to be detected for reaction, enabling the virus to be detected to be completely specifically combined with the antibody, and simultaneously diluting the virus stock solution to the virus concentration C₁、C₂……C_mVirus standard solution of (3), C₁、C₂……C_mSuccessively increase, C₁≤5ng/ml，C_m≥155ng/ml；

3. Adding a sealing reagent into one pair of reagent bottles, adding deionized water and PBS buffer solution into one pair of reagent bottles, and adding V and C into one reagent bottle₁the virus standard solution of (2) is to contain C₁The reagent bottle of the virus standard solution is marked as a sample bottle;

4. controlling a valve control mechanism to enable two reagent bottles containing a closed reagent to be communicated with the inlet of the microfluidic chip, enabling the two reagent bottles containing the closed reagent to be respectively communicated with the buffer solution moving channel and the sample moving channel, starting an air pump, respectively introducing the closed reagent into the buffer solution moving channel and the sample moving channel to reduce non-specific adsorption, and after the closing is finished, controlling the valve control mechanism to enable the two reagent bottles containing the closed reagent to be separated from the inlet of the microfluidic chip;

5. Controlling a valve control mechanism to enable two reagent bottles containing deionized water and PBS buffer solution to be communicated with the inlet of the microfluidic chip, enable the reagent bottle containing PBS buffer solution to be communicated with a buffer solution moving channel, enable the reagent bottle containing deionized water to be communicated with a sample moving channel, enable the PBS buffer solution to be introduced into the buffer solution moving channel, introduce an ionic water sample into the moving channel, enable a cleaning buffer solution moving channel to be communicated with the sample moving channel, and after cleaning, controlling the valve control mechanism to enable the two reagent bottles containing deionized water and PBS buffer solution to be separated from the inlet of the microfluidic chip;

6. Controlling a valve control mechanism to enable two reagent bottles containing virus standard solution and PBS buffer solution to be communicated with the inlet of the microfluidic chip, enabling the reagent bottle containing the virus standard solution to be communicated with a sample moving channel, enabling the reagent bottle containing the PBS buffer solution to be communicated with a buffer solution moving channel, introducing the PBS buffer solution into the buffer solution moving channel, introducing virus standard solution into the sample moving channel, enriching the magnetic microspheres in the virus standard solution in the buffer solution moving channel and flowing into the capillary glass tube, capturing the magnetic microspheres at the capillary glass tube, when the virus standard solution is completely introduced, the control valve control mechanism is controlled to separate the reagent bottle containing the virus standard solution from the inlet of the microfluidic chip, when the voltage at the two ends of the photosensitive element is not changed any more, the control valve control mechanism cuts off the reagent bottle containing the PBS buffer solution from the inlet of the microfluidic chip and records the voltage V at the two ends of the photosensitive element.₁；

7. Cleaning the sample bottle, adding V volume and C concentration into the sample bottle₂the virus standard solution of (4); or directly adding V volume and C concentration into one of the reagent bottles₂The virus standard solution of (4);

8. Repeating the steps 8.4-8.6 to obtain a voltage V across the photosensitive element₂；

9. repeating the steps 8.7-8.8 until all the virus standard solutions are detected, and respectively measuring the voltage V at the two ends of the photosensitive element₃……V_m；

10. Taking the concentration of the virus standard solution as an abscissa and the resistance at two ends of the photosensitive element as an ordinate, drawing according to detected data, fitting to obtain a standard curve, and obtaining a functional relation y ═ k of the standard curve according to the standard curve₁x+b₁；

11. and (3) detecting the virus liquid to be detected by using the method of the step 8.3-8.6, measuring the voltage at two ends of the photosensitive element as V ', and substituting the V' into the functional relation to obtain the concentration of the virus in the virus liquid to be detected.

Compared with the prior art, the invention has the beneficial effects and advantages that:

1. The invention realizes the automation of virus detection, can accurately inject reagents, reduces the waste of the reagents in manual operation, and can greatly reduce errors or wrong diagnosis caused by manual operation errors.

2. The invention realizes the separation of virus protein by utilizing the magnetism of the magnetic microspheres, judges the virus concentration in the solution by utilizing the black property of the magnetic spheres and combining the volt-ampere characteristic of the photoresistor, integrates the enrichment and content detection of the virus protein on the same equipment, and simplifies the complicated operation process in the traditional detection process.

3. the invention has simple realization, low cost, high efficiency and no high-end technical requirement, and effectively simplifies the complex process flow of the traditional virus detection.

4. The invention can be applied to preliminary diagnosis in the early stage, can reduce the cost of precision instrument inspection, can reduce unnecessary waste and saves the detection cost.

Drawings

Fig. 1 is a schematic structural view of a virus detection sensor.

fig. 2 is a schematic view of the structure of the detection body.

Fig. 3 is a circuit diagram of the photoelectric conversion circuit.

FIG. 4 is a schematic diagram of the structure of the apparatus for detecting the concentration of viruses.

FIG. 5 is a schematic diagram of a valve control chip.

Fig. 6 is an exploded view of the valve core plate.

fig. 7 is a schematic structural diagram of the microfluidic chip.

FIG. 8 is a graph showing a relationship between a virus concentration in a virus standard solution and a voltage across a photoresistor.

fig. 9 is a standard graph.

Wherein, 1-capillary glass tube, 2-detecting body, 3-C type clamp, 4-supporting body, 5-supporting plate, 6-first groove, 7-second groove, 8-light emitting diode, 9-photoresistor, 10-third groove, 11-air pump, 12-first air suction branch tube, 13-first valve, 14-first reagent bottle, 15-second reagent bottle, 16-third reagent bottle, 17-fourth reagent bottle, 18-fifth reagent bottle, 19-micro-fluidic chip, 20-multi-channel pneumatic electromagnetic valve, 21-controller, 22-valve control chip, 23-Diwen screen, 24-sample discharging tube, 25-waste discharging tube, 26-second air suction branch tube, 27-second valve, 28-sample discharging tube, 29-communicating pipe, 30-buffer solution moving channel, 31-sample moving channel, 32-first injection port, 33-second injection port, 34-first discharge port, 35-second discharge port, 36-first clamping plate, 37-second clamping plate, 38-liquid inlet pipe, 39-liquid outlet pipe, 40-air inlet pipe, 41-cover plate, 42-organic silicon film, 43-substrate, 44-first liquid inlet hole, 45-first liquid outlet hole, 46-gas through groove, 47-second liquid inlet hole, 48-second liquid outlet hole, 49-first main channel, 50-first liquid inlet separating channel, 51-first liquid outlet channel, 52-second main channel, 53-second liquid outlet channel, 54-second liquid inlet separating channel, 55-first waste liquid separating bottle, 56-second waste bottle.

Detailed Description

The invention is described in detail below with reference to the figures and specific embodiments.